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Stem Cell Intel
Issue 5 | 2015
Each quarter, Stem Cell Intel provides you with the latest product news, a column from one of our experts,
upcoming industry events, and easy access to technical tools such as publications, protocols, FAQs, and more.
To subscribe to Stem Cell Intel, go to lifetechnologies.com/stemcellintel
Product news
Single cell passaging for PSCs
RevitaCell™ Supplement was developed for use with Essential 8™ Medium to enable
maximum cell viability when performing single cell passaging of pluripotent stem
cells (PSCs). RevitaCell™ Supplement includes a more specific ROCK inhibitor than
traditional ROCK inhibitors and is coupled with molecules containing antioxidant
and free radical–scavenger properties. Together, these products provide a system
that minimizes the impact of stress from single cell passaging—enabling maximum
cell viability and more efficient recovery of cells while retaining normal morphology,
pluripotency, and karyotype.
Discover this single cell passaging system >>
Definitive endoderm cells in 48 hours—can your protocol do that?
The PSC Definitive Endoderm Induction Kit is a fast way to generate high-quality,
highly pure definitive endoderm cells from human pluripotent stem cells (hPSCs).
The kit consists of two animal origin–free media, each supplied as a 1X complete
medium, requiring no mixing of additional components. Unlike other methods that
require multiple components and take five or more days, the PSC Definitive Endoderm
Induction Kit can generate ≥90% CXCR4+/PDGFRα definitive endoderm cells that
express the key markers Sox17 and FoxA2. The functional cells created from this kit
are capable of differentiating into midgut/hindgut, pancreatic endoderm, or liver bud
progenitors that express relevant physiological markers.
See the data and compare your protocol >>
Maximize PSC cryopreservation and recovery
The PSC Cryopreservation Kit contains a xeno-free cryopreservation medium
and a chemically defined recovery supplement. When used in combination, these
reagents help minimize loss of cell viability, maximize post-thaw recovery, and
minimize unwanted differentiation of PSCs. The PSC Cryopreservation Kit is designed
to consistently recover 50% more viable cells post-thaw compared to traditional
methods of cryopreservation.
Order now >>
Technical highlights
PSC Cardiomyocyte Differentiation Kit—more efficient path to
contracting cardiomyocytes
The PSC Cardiomyocyte Differentiation Kit enables efficient differentiation of hPSCs to contracting
cardiomyocytes in as few as 8 days. A newly developed protocol achieves even better cardiomyocyte
differentiation efficiency: singularizing cells prior to differentiation allows for better seeding and confluency
estimates, which have been shown to be crucial in maximizing differentiation efficiency.
See details in the user bulletin under “Manuals & protocols” >>
Publication: Characterizing pluripotent stem cells using the
TaqMan® hPSC Scorecard™ Panel
We recently published a paper in Methods in Molecular Biology that featured the TaqMan® hPSC Scorecard™ Panel.
“Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC Scorecard™ Panel” demonstrates the utility
of the panel for rapid characterization of PSC lines. It has been chosen by Global Medical Discovery as a key
scientific article contributing to excellence in biomedical research and was highlighted in their February online
Read the publication abstract >>
Upcoming events
Genome Editing Applications Conference
March 18–19, 2015
Boston, MA
The IBC Life Sciences Genome Editing Applications conference will showcase the most up-to-date therapeutic
and biomedical applications emerging using CRISPRs, ZFNs, TALENs, AAV, and other genome engineering
technologies. From validation of targeted cell lines and development of transgenic animal models to
therapeutic uses in cell therapy, gene therapy, and immunotherapy, this event will also highlight applications in
drug target identification/validation and lead discovery and explore how these technologies are being used for
whole-genome and functional screening as well as basic research applications.
Stem Cell Training Workshop
April 28–30, 2015
Glasgow, UK
This workshop provides researchers with hands-on training in techniques for culturing and characterizing
human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), as well as reprogramming
techniques for the creation of iPSCs. Whether you’re new to PSC research or need a refresher course, our R&D
scientists can provide detailed training so that you can feel confident using stem cells in your research.
Find out more at lifetechnologies.com/stemcellintel
For Research Use Only. Not for use in diagnostic procedures. © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered
trademark of Roche Molecular Systems, Inc., used under permission and license. Essential 8 is a trademark of Cellular Dynamics International, Inc. CO121662 0315