Catalogue of Research Project Proposals and Potential Participants

Catalogue of
Research Project Proposals
and Potential Participants
Results of the MedResIn Web-Survey
The survey is open for further contribution.
Please visit: http://www.meduni-graz.at/medresin
Editorial
This catalogue was assembled from research profiles provided in the on-line survey of
MedResIn at http://www.meduni-graz.at/medresin/profiles.html.
It is understood that all information provided is part of the intellectual property of the
submitter.
Disclaimer
Please note that the responsibility for the content and formulation of the abstracts lies
with the submitters of the abstracts.
All content of the MedResIn survey has been compiled carefully. The Medical University of
Graz makes no guarantees of accuracy, completeness and timeliness of the information
provided by the participants. Therefore the Medical University of Graz accepts no
responsibility or liability for damages or losses resulting from the use of this website.
Contact
Dr. Carolin Auer
Medical University of Graz
http://www.meduni-graz.at/medresin
Introduction
The Specific Support Action "Medical Research Initiative South Eastern Europe MedResIn" has been set up to strengthen scientific & technological interaction and cooperation in biomedical research between the EU and South Eastern Europe, especially
the Western Balkan countries. Its aim is to facilitate future participation of Medical
Universities/Faculties and Schools from Western Balkan countries in the relevant
Priorities of the European Framework Programmes. Specifically, it is targeted towards
creating a platform for the transition to FP7 in order to provide best possible starting
conditions for biomedical research in Western Balkan countries, thus supporting their
integration into the European Research Area (ERA).
The MedResIn project endeavors to
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increase visibility & accessibility of biomedical research communities from Western
Balkan countries in Europe;
increase the access of Western Balkan countries biomedical research communities
to the FP by providing tailor-made capacity building measures following their
specific needs;
support the development of joint EU research projects including partners from
Western Balkan countries;
build up a sustainable network of institutions which will keep up co-operation;
better, faster and balanced implementation of Life Sciences and biomedical
research-related priorities of the FP in Western Balkan countries.
The MedResIn extended consortium currently consists of partners from 8 countries,
including Western Balkan participants as well as EU member states.
The initiative is managed by a core consortium of 6 partners:
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Medical University of Graz (Coordinator)
University of Maribor, Medical Faculty
University of Zagreb, School of Dental Medicine
University of Belgrade, Medical Faculty
RTD Services
University of Padova, Medical Faculty
Table of Contents
Institutional Profiles
8
Individual Profiles of Researchers and Teams
Anesthaesiology
Anatomy
Bacteriology
Biochemistry
Biology
Biophysics
Clinical Chemistry
Clinical Microbiology
Cytology
Dentistry
Dermatology
Epidemiology
Genetics
Immunology and Immunohaematology
Information Technology
Internal Medicine
Microbiology
Neurology
Obstetrics and Gynaecology
Other Allied Sciences
Pathology
Pediatrics
Pharmacology
Physiology
Psychiatry
Public Health Services
Radiology
Social Medicine
Surgery
Therapeutics
Toxicology
55
56
57
64
66
89
104
107
108
110
114
130
131
140
168
186
189
209
215
217
219
220
228
234
244
254
256
263
268
269
282
293
Institutions
Institutions
Institution/University
Name of Institution/University
Brodarski Institute
Number of Employees
170
Number of Researchers
50
Country
Croatia
Postal Adress
Av. V. Holjevca 20
10020 Zagreb
Projects
Domestic Projects
7
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 6504110
Fax
+385 1 6504280
Website
www.hrbi.hr
Research Team Objectives
Main Fields
thermal analysis of materials
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Children´s Hospital Zagreb
Number of Employees
638
Number of Researchers
Country
Croatia
Postal Adress
Klaićeva 16
10000 Zagreb
Projects
Domestic Projects
6
International Projects
4
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4600100
Fax
+385 1 4826053
Website
http://kdb.hr
Research Team Objectives
Main Fields
biomedicine, health, paediatrics
Partners/Interests
biomedicine, health, paediatrics
Institutions
Institution/University
Name of Institution/University
Clinic for Traumatology
Number of Employees
447
Number of Researchers
18
Country
Croatia
Postal Adress
Draškovićeva 19
10000 Zagreb
Projects
Domestic Projects
2
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4697000
Fax
+385 1 4610365
Website
www.trauma.hr
Research Team Objectives
Main Fields
biomedicine and health, biology, biotechnology
Partners/Interests
clinical biomedicine, biochemistry, biotechnology
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Karlovac”
Number of Employees
889
Number of Researchers
20
Country
Croatia
Postal Adress
A. Štampara 3
47000 Karlovac
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 47 608100
Fax
+385 47 431337
Website
Research Team Objectives
Main Fields
biomedicine and health
Partners/Interests
oncology, immunology, genetics, endocrinology, clinical chemistry, ophthalmology, cardiology &
vascular diseases, metabolism, dermatology, neuroscience, traumatology, urology
Institutions
Institution/University
Name of Institution/University
Clinical Hopital “Merkur”
Number of Employees
820
Number of Researchers
Country
Croatia
Postal Adress
Zajčeva 19, p.o. box 414
10000 Zagreb
Projects
Domestic Projects
6
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 2431396
Fax
+385 1 2431402
Website
www.kb-merkur.hr
Research Team Objectives
Main Fields
biomedicine and health
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Osijek”
Number of Employees
2802
Number of Researchers
114
Country
Croatia
Postal Adress
Josipa Huttlera 4
31000 Osijek
Projects
Domestic Projects
5
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 31 511106
Fax
+385 31 512235
Website
www.kbo.hr
Research Team Objectives
Main Fields
biomedicine and health
Partners/Interests
biomedicine and health
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Požega”
Number of Employees
507
Number of Researchers
19
Country
Croatia
Postal Adress
Osječka 107
34000 Požega
Projects
Domestic Projects
5
International Projects
2
Contact Person
Name
Email
[email protected]
Function
Phone
+385 34 254555
Fax
+385 34 271713
Website
Research Team Objectives
Main Fields
biomedicine and health
Partners/Interests
oncology, immunology, genetics, endocrinology, clinical chemistry, ophthalmology, cardiology &
vascular diseases, metabolism, dermatology, neuroscience
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Sisters of charity”
Number of Employees
2293
Number of Researchers
116
Country
Croatia
Postal Adress
Vinogradska cesta 29
10000 Zagreb
Projects
Domestic Projects
22
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 3787110
Fax
+385 1 3768269
Website
www.kbsm.hr
Research Team Objectives
Main Fields
biomedicine
Partners/Interests
oncology, immunology, genetics, endocrinology, clinical chemistry, ophthalmology, cardiology &
vascular diseases, metabolism, dermatology, neuroscience
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Split”
Number of Employees
3120
Number of Researchers
158
Country
Croatia
Postal Adress
Spinčećeva 1
21000 Split
Projects
Domestic Projects
7
International Projects
1
Contact Person
Name
Email
[email protected]
Function
Phone
+385 21 556111
Fax
+385 21 365738
Website
www.kbsplit.hr
Research Team Objectives
Main Fields
biomedicine, clinical medicine, public health
Partners/Interests
partners with similar research interests, multicentric clinical studies
interventional radiology, cardiology, oncology, molecular pathology, nephrology, ophthalmology,
gynecology, endoscopic surgery, hepatitis, war effect on health, glucose-6-phosphate deficiency,
asbestosis
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Sveti Duh”
Number of Employees
1311
Number of Researchers
34
Country
Croatia
Postal Adress
Sveti Duh 64
10000 Zagreb
Projects
Domestic Projects
9
International Projects
78
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 3712111
Fax
+385 1 3712308
Website
www.obsd.hr
Research Team Objectives
Main Fields
perinatology, otorinolanringology, neurology, urology gynecology, ophthalmology, surgery, internal
medicine
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Clinical Hopital Center Zagreb
Number of Employees
4431
Number of Researchers
56
Country
Croatia
Postal Adress
Šalata 2
10000 Zagreb
Projects
Domestic Projects
15
International Projects
86
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4920019
Fax
+385 1 4818457
Website
www.kbc-zagreb.hr
Research Team Objectives
Main Fields
biomedicine
Partners/Interests
oncology, immunology, genetics, endocrinology, clinical chemistry, ophthalmology, cardiology &
vascular diseases, metabolism, dermatology, neuroscience, oral medicine, denta materials,
orthodontics, periodontology
Institutions
Institution/University
Name of Institution/University
Clinical Hospital for Chronic Diseases in Children- Zagreb
Number of Employees
150
Number of Researchers
12
Country
Croatia
Postal Adress
Srebrnjak 100
10000 Zagreb
Projects
Domestic Projects
1
International Projects
2
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 2430783
Fax
+385 1 2430784
Website
www. .hr
Research Team Objectives
Main Fields
biomedicine, pharmacy, biochemistry
Partners/Interests
biomedicine, pharmacy, biochemistry
Institutions
Institution/University
Name of Institution/University
Clinical Hospital for Diabetes, Endocrinology and Metabolic Diseases
“Vuk Vrhovec”
Number of Employees
258
Number of Researchers
32
Country
Croatia
Postal Adress
Dugi Dol 4a
10000 Zagreb
Projects
Domestic Projects
6
International Projects
2
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 2353800
Fax
+385 1 2331515
Website
www.idb.hr
Research Team Objectives
Main Fields
biomedicine, diabetology, endocrinology, behavioural medicine
Partners/Interests
research areas related to diabetes and endocrinological diseases
biomedicine, diabetology, endocrinology, behavioural medicine
Institutions
Institution/University
Name of Institution/University
Clinical Hospital for Psychiatry “Sveti Ivan”
Number of Employees
320
Number of Researchers
9
Country
Croatia
Postal Adress
Jankomir 1
10090 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 3430000
Fax
+385 1 3794116
Website
www.pbsvi.hr
Research Team Objectives
Main Fields
biomedicine, psychiatry, psychosis, alcoholism
Partners/Interests
biomedicine, psychiatry, psychosis, alcoholism
Institutions
Institution/University
Name of Institution/University
Clinical Hospital for Tumors
Number of Employees
434
Number of Researchers
39
Country
Croatia
Postal Adress
Ilica 197
10000 Zagreb
Projects
Domestic Projects
3
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 3783555
Fax
+385 1 3775536
Website
www.kzt.hr
Research Team Objectives
Main Fields
oncology
Partners/Interests
oncology
Institutions
Institution/University
Name of Institution/University
Croatian Institute for Brain Research
Number of Employees
28
Number of Researchers
5
Country
Croatia
Postal Adress
Šalata 12
10000 Zagreb
Projects
Domestic Projects
41
International Projects
14
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4596902
Fax
+385 1 459642
Website
www.hiim.hr
Research Team Objectives
Main Fields
brain research
Partners/Interests
research areas related to brain research
inquiry including normal development of the human cerebral cortex, developmental brain disorders,
signalling mechanisms and molecules in the developing and adult brain, the CSF pathophysiology and
hydrocephalus, neurodegenerative diseases and schizophrenia, brain plasticity and repair after
perinatal hypoxic/ischaemic lesions in premature infants
Institutions
Institution/University
Name of Institution/University
Croatian Veterinary Institute
Number of Employees
203
Number of Researchers
45
Country
Croatia
Postal Adress
Savska cesta 143, p.o. box 883
10000 Zagreb
Projects
Domestic Projects
8
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 6190845
Fax
+385 1 6190841
Website
www.veinst.hr
Research Team Objectives
Main Fields
veterinary medicine
Partners/Interests
veterinary medicine
Institutions
Institution/University
Name of Institution/University
Faculty of Medicine of the University of Osijek
Number of Employees
113
Number of Researchers
89
Country
Croatia
Postal Adress
Josipa Huttlera 4, p.o. box 392
31000 Osijek
Projects
Domestic Projects
21
International Projects
4
Contact Person
Name
Email
[email protected]
Function
Phone
+385 31 512888
Fax
+385 31 512833
Website
www.mefos.hr
Research Team Objectives
Main Fields
biomedicine and health, biochemistry, physics, physiology and immunology, biometrics and
biomechanics, genetics and forensic medicine, pharmacology of pain, neurobiology, embryology,
nanomedicine
Partners/Interests
basic and clinical medical sciences, industry
physiology, general and cardiovascular physiology, genetics, pharmacology, immunology (kidney
transplantation), basic medical sciences, gastroenterology, cardiology, neurology, psychiatry
Institutions
Institution/University
Name of Institution/University
Faculty of Medicine of the University of Rijeka
Number of Employees
495
Number of Researchers
338
Country
Croatia
Postal Adress
Braće Branchetta 20
51000 Rijeka
Projects
Domestic Projects
52
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 51 65111
Fax
+385 51 675806
Website
www.medri.hr
Research Team Objectives
Main Fields
biomedicine and health, oral science
Partners/Interests
oral sciences
temporomandibular disorders, dental materials, biological effects of dental materials, root channel
instrumentation, apex locators, influence of restorative procedures on dental pulp, chronic orofacial
pain, esthetic fixed prostethic dentistry, periodontology, cariology, clinical antrophology,
morphometric, and craniometric analysis of malocclusion, dental traumatology, oral health in children
with (and without) disabilities, early childhood caries
Institutions
Institution/University
Name of Institution/University
Faculty of Pharmacy and Biochemistry of the University of Zagreb
Number of Employees
11,3
Number of Researchers
82
Country
Croatia
Postal Adress
A. Kovačića 1, p.o. box 156
10000 Zagreb
Projects
Domestic Projects
20
International Projects
14
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4856201
Fax
+385 1 4856201
Website
www.pharma.hr
Research Team Objectives
Main Fields
pharmacy, biochemistry, molecular biology, chemistry
Partners/Interests
pharmacy, biochemistry, molecular biology, chemistry
Institutions
Institution/University
Name of Institution/University
Faculty of Veterinary Medicine of the University of Zagreb
Number of Employees
318
Number of Researchers
162
Country
Croatia
Postal Adress
Heinzelova 55
10000 Zagreb
Projects
Domestic Projects
46
International Projects
13
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 2390111
Fax
+385 1 2441390
Website
www.vef.hr
Research Team Objectives
Main Fields
veterinary medicine
Partners/Interests
veterinary medicine
Institutions
Institution/University
Name of Institution/University
Institute for Medical Research and Occupational Health
Number of Employees
150
Number of Researchers
51
Country
Croatia
Postal Adress
Ksaverska cesta 2, p.o.box 291
10000 Zagreb
Projects
Domestic Projects
29
International Projects
12
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4673188
Fax
+385 1 4673303
Website
www.imi.hr
Research Team Objectives
Main Fields
working and living environment, hygiene, health and dissemination of knowledge on industrial
hygiene, environmental population and radiation
Partners/Interests
biomedicine, ecology, biology, hygiene
Institutions
Institution/University
Name of Institution/University
Institute of Immunology, Inc.
Number of Employees
360
Number of Researchers
75
Country
Croatia
Postal Adress
Rockefellerova 2. p.o. box 266
10000 Zagreb
Projects
Domestic Projects
6
International Projects
2
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4684500
Fax
+385 1 468433
Website
www.imz.hr
Research Team Objectives
Main Fields
molecular biomedicine, cellular immunology, radioimmunology and chemistry
Partners/Interests
immunology, biochemistry
Institutions
Institution/University
Name of Institution/University
PLIVA Research Institute Ltd.
Number of Employees
130
Number of Researchers
97
Country
Croatia
Postal Adress
Prilaz baruna Filipovica 29
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 37211889
Fax
+385 1 3721534
Website
www.pliva.hr
Research Team Objectives
Main Fields
medical chemistry, pharmacology, biology, drug design, preclinical research
Partners/Interests
medical chemistry, pharmacology, biology, drug design, preclinical research
Institutions
Institution/University
Name of Institution/University
Public Health Institute
Number of Employees
408
Number of Researchers
33
Country
Croatia
Postal Adress
Mirogojska cesta 16
10000 Zagreb
Projects
Domestic Projects
1
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4696151
Fax
+385 1 4678002
Website
www.publichealth-zagreb.hr
Research Team Objectives
Main Fields
public health, biology
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Ruđer Bošković Institute
Number of Employees
824
Number of Researchers
516
Country
Croatia
Postal Adress
Bijenička cesta 54, p.o. box 180
10000 Zagreb
Projects
Domestic Projects
125
International Projects
59
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4561111
Fax
+385 1 4680084
Website
www.irb.hr
Research Team Objectives
Main Fields
biomedicine, biology, oceanography, physics, chemistry, computing, engineering
Partners/Interests
Institutions
Institution/University
UZAG
Name of Institution/University
School of Dental Medicine, University of Zagreb
Number of Employees
256
Number of Researchers
146
Country
Croatia
Postal Adress
Gunduliceva 5, p.o. box 756
10000 Zagreb
Projects
Domestic Projects
20
International Projects
2
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4802123
Fax
+385 1 4830804
Website
www.sfzg.hr
Research Team Objectives
Main Fields
oral sciences
Partners/Interests
oral sciences
dental anthropology, oral medicine, dental materials, orthodontics, periodontology
Institutions
Institution/University
Name of Institution/University
School of Medicine University of Zagreb
Number of Employees
763
Number of Researchers
495
Country
Croatia
Postal Adress
Šalata 3
10000 Zagreb
Projects
Domestic Projects
120
International Projects
7
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4566909
Fax
+385 1 4566724
Website
www.mef.hr
Research Team Objectives
Main Fields
anatomy,
anaesthesiology,
cytology,
histology,
embryology,
dermatology,
epidemiology,
pharmacology, toxicology, physical medicine and rehabilitation, physiology, genetics, gynecology,
obstetrics, hygiene, immunology, internal medicine, public health, surgery, chemistry, biochemistry,
microbiology, biology, neurology, neuroscience, nuclear medicine, ophthalmology, oncology,
orthopedics, otorhynolaryngology, pathology, pediatrics, psychiatrics, radiology, social medicine,
forensic medicine, urology, health ecology
Partners/Interests
anatomy,
anaesthesiology,
cytology,
histology,
embryology,
dermatology,
epidemiology,
pharmacology, toxicology, physical medicine and rehabilitation, physiology, genetics, gynecology,
obstetrics, hygiene, immunology, internal medicine, public health, surgery, chemistry, biochemistry,
microbiology, biology, neurology, neuroscience, nuclear medicine, ophthalmology, oncology,
orthopedics, otorhynolaryngology, pathology, pediatrics, psychiatrics, radiology, social medicine,
forensic medicine, urology, health ecology
Institutions
Institution/University
SUVAG
Name of Institution/University
Polyclinic for the Rehabilitation of Listening and Speech
Number of Employees
264
Number of Researchers
40
Country
Croatia
Postal Adress
Ul. Kneza Ljudevita Posavskog 10, p.o. box 617
10000 Zagreb
Projects
Domestic Projects
4
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4629600
Fax
+385 1 4655166
Website
www.suvag.hr
Research Team Objectives
Main Fields
physiology of listening and speech, rehabilitation of listening an speech, cochlear implants
Partners/Interests
epidemiology, diagnostics and rehabilitation of listening and speech disorders
Institutions
Institution/University
UMAR
Name of Institution/University
Faculty of Medicine, University of Maribor
Number of Employees
28
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomškov trg 15
2000 Maribor
Projects
Domestic Projects
4
International Projects
3, EU 6-FP-IP PATHOGENCOMBAT FOOD-CT-2005-007081, EU 6FP-MEDRESIN-2005, Network for E-Learning in Medical Education eContentplus - IST
Contact Person
Name
[email protected]
[email protected]
[email protected]
Email
Function
+386 22345601
Phone
+386 22345600
Fax
www.mf.uni-mb.si
Website
Research Team Objectives
Main Fields
biomedicine
Partners/Interests
other biomedical research institutes
Function of Endocrine Cells in Disease and Compensatory Processes - ARRS (prof. Rupnik)
http://sicris.izum.si/search/rsr.aspx?lang=eng&id=7683&opt=3
Genetic Susceptibility to Gastrointestinal Complex Diseases and Pharmacogenomics - ARRS (doc.
Potočnik) http://sicris.izum.si/search/rsr.aspx?opt=3&lang=eng&id=9398
PathogenCombat – EU 6th FP (doc. Cencič) http://www.pathogencombat.com/
Center of excellence – Biotechnology with pharmacy – EU Foundation for Regional Development
(prof. Štrukelj) http://www.gov.si/euskladi/skladi/strukt_esrr_3.html
Network
for
E-Learning
in
Medical
Education
eContentplus,(dr.
Dinevski)
http://europa.eu.int/information_society/activities/econtentplus/index_en.htm
Incidence of erythropoietic protoporphyria and molecular mechanism in population of Slovenia - ARRS
(prof. Krajnc) http://sicris.izum.si/search/rsr.aspx?lang=eng&id=4431&opt=3
Institutions
Institution/University
KOPA
Name of Institution/University
University Clinic of Respiratory of Allergic Diseases
Number of Employees
454
Number of Researchers
50
Country
Slovenia
Postal Adress
Golnik 36
4204 Golnik
Projects
Domestic Projects
10
International Projects
1,5FP EU BIOMED, No. QLRT-2001-0893
Contact Person
Name
Email
[email protected]
[email protected]
Function
Phone
+ 386 4 2569 111
Fax
+ 386 4 2569 117
Website
www.klinika-golnik.si
Research Team Objectives
Main Fields
Pulmology and Allergology
Partners/Interests
Institutions
Institution/University
Name of Institution/University
General Hospital Ptuj
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Potrčeva 23-25
2250 Ptuj
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+386 2 749 14 00
Fax
+386 2 749 16 30
Website
Research Team Objectives
Main Fields
Dilatative Myocardiopathy
Partners/Interests
Institutions
Institution/University
Name of Institution/University
General Hospital Maribor
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Ljubljanska 5
2000 Maribor
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
www.sb-mb.si/index.php?id=kontakt
Function
Phone
+ 386 2 32 11 000
Fax
+ 386 2 33 12 393
Website
http://www.sb-mb.si/index.php?id=2
Research Team Objectives
Main Fields
Partners/Interests
1. Ph.D. Vlaisavljević
Human reproduction (gynecology, andrology, perinatology, ultrasound, mammography, embryology laboratory, IVF, micromanipulation,genetics, diagnostics, breast diseases, infertility, endocrinology)
http://sicris.izum.si/search/rsr.aspx?lang=eng&id=6601&opt=3
Project: Charactheristics of development, maturation and fertilization capacity of oocytes in ivf and
icsi procedures in natural cycle,
Acronym: J3-8764 (C)
Duration: 1.1.1997 - 31.12.1999,
Keywords: IVF, ICSI, natural cycle, fertilization, maturation in vitro, fertilization failure, follicleultrasound, follicle-Doppler, estradiol monitoring,
Project: Cytogenetic analysis of human oocytes and preimplantation embryos
Acronym: L3-5355 (C)
Duration: 1.1.2003 - 31.12.2005
RANGE: 0,29 FTE
Keywords: preimplantation genetic diagnosis, fluorescence in situ hybridization, oocytes, embryos
Project: Value of transvaginal hydrolaparoscopy in infertility evaluation
Acronym: L3-5246 (C)
Duration: 1.1.2003 - 31.12.2005
RANGE: 0,29 FTE
Keywords: transvaginal hydrolaparoscopy, hysterosalpingography, infertility evaluation
Institutions
Institution/University
Name of Institution/University
General Hospital Celje
Number of Employees
1613
Number of Researchers
Country
Slovenia
Postal Adress
Oblakova ulica 5
3000 Celje
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+386 3 423 3000
Fax
+386 3 423 3666
Website
www.sb-celje.si
Research Team Objectives
Main Fields
Partners/Interests
R.Komadina
Medical sciences / Neurobiology, Gerontologic trauma, major trauma
http://sicris.izum.si/search/rsr.aspx?opt=3&lang=eng&id=11437
Project: Trauma Register
Acronym: L3-2037 (C)
Duration: 1.1.2000 - 31.12.2001
Keywords: scoring systems, registry, multiple injured patients
Project: Genetic factors and hormones in metabolic diseases
Acronym: J3-3314 (C)
Duration: 1.7.2001 - 30.6.2004
RANGE: 0,63 FTE and 417 unpaid hours
Keywords: osteoporosis, arthroplasty, diabetic nephropathy, insulin resistance
Project: Telederm - Modern diagnostic of skin tumour and selected dermathoses
Acronym: L3-3426 (C)
Duration: 1.7.2001 - 30.6.2004
Keywords: Skin tumours, Dermatosis, Internet, Diagnostics
Institutions
Institution/University
Name of Institution/University
General Hospital Slovenj Gradec
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Gosposvetska 1
2380 Slovenj Gradec
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+386 2 8823400
Fax
+386 2 8823411
Website
http://www.sb-sg.si/
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Dubrava”
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Avenija Gojka Šuška 6
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 2902444
Fax
+385 1 2860259
Website
http://www.kbd.hr
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Clinical Hospital “Šibenik”
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Stjepana Radića 83
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 22 246246
Fax
+385 22 214707
Website
http://www.bolnica-sibenik.htnet.hr
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Clinical Hospital for Infectious Diseases “Dr. Fran Mihaljević”
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Mirogojska 8
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4603222
Fax
+385 1 4678235
Website
http://www.bfm.hr
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Clinical Hospital for Psychiatry “Vrape”
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Bolnička c.32
10090 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 3780666
Fax
+385 1 3483660
Website
http://www. bolnica-vrapce.hr
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Clinical Hospital for Pulmonary Diseases “Jordanovac”
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Jordanovac 104
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 2385100
Fax
+385 1 213045
Website
http://jagor.srce.hr/jordanovac
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Croatian Academy of Sciences and Arts
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Zrinski trg 11
10000 Zagreb
Projects
Domestic Projects
3
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4895111
Fax
+385 1 4819979
Website
http://www.hazu.hr
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Croatian Academy of Sciences and Arts – Cabinet for the Research
and Standardization of Immunological Substances
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Demetrova 18
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 443586
Fax
Website
http://mahazu.hazu.hr/ENG/Cab_ResSt_ImmS.html
Research Team Objectives
Main Fields
immunologically active substances in viral precancerous, cancerous, and other diseases
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Croatian Academy of Sciences and Arts – Cabinet for the Research
of the Structure and Function of the Sensory Organs
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Ante Kovačića 5
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4698246
Fax
+385 1 4856211
Website
http://mahazu.hazu.hr/ENG/Cab_ResS-F_Sen-O.html
Research Team Objectives
Main Fields
sensory neuropsychology
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Croatian Academy of Sciences and Arts – Department of Medical
Sciences
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Andrije Hebranga 1
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4895171
Fax
+385 1 4819979
Website
http://www.hazu.hr/Raz4.html
Research Team Objectives
Main Fields
professional health hazards, oncogenic and growth factors, tumors, occupational medicine and
medical ecology, animal and comparative pathology, allergology and clinical immunology, biological
substances, drugs, cardiovascular diseases, subcellular pathology, atherosclerosis, parodontal
diseases, and biomedical ethics
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Faculty of Kinesiology of the University of Zagreb
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Horvaćanski zavoj 15
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 3658666
Fax
+385 1 3634146
Website
http://www.kif.hr
Research Team Objectives
Main Fields
kinesiology
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Faculty of Medicine of the University of Split
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Šoltanska 2
21000 Split
Projects
Domestic Projects
16
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 21 557900
Fax
+385 21 557625
Website
http://www.mefst.hr
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Faculty of Science of the University of Zagreb
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Horvatovac 102A
10000 Zagreb
Projects
Domestic Projects
3
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4606010
Fax
+385 1 4606013
Website
http://www.pmf.hr
Research Team Objectives
Main Fields
biology, physics, chemistry, mathematics ,geophysics, geography, geology
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Health Polytechnic in Zagreb
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Mlinarska 38, p.o. box 901
10000 Zagreb
Projects
Domestic Projects
1
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4669750
Fax
+385 1 4668080
Website
http://www.zvu.hr
Research Team Objectives
Main Fields
Partners/Interests
Institutions
Institution/University
Name of Institution/University
Institute for Anthropological Research
Number of Employees
Number of Researchers
Country
Croatia
Postal Adress
Amruševa 8, p.o. box 290
10000 Zagreb
Projects
Domestic Projects
International Projects
Contact Person
Name
Email
[email protected]
Function
Phone
+385 1 4816903
Fax
+385 1 4813777
Website
http://www.pmf.hr
Research Team Objectives
Main Fields
anthropology, mathematical modelling and biostatistics, human ecology, population genetics,
ergonomics, anthropological biometrics, biomechanics, occupational medicine, anthropological
anatomy, epidemiology, health prevention, social medicine, forensics, archaeology, anthropogeographics, general ecology
Partners/Interests
Individual Researchers and Teams
Anaesthesiology
Research Team
Research Team Name
ICU
Individual Researcher
No
Research Team Leader Name
Prof. Bruno Barsic
Research Team Members
Team Member 0
dr Igor Klinar []
Team Member 1
dr Dragan Lepur []
Team Member 2
dr Marija Santini []
Team Member 3
dr Vladimir Krajinovic []
Team Member 4
dr Marko Kutlesa []
Contact Person
Name
Prof. Bruno Barsic
Email
[email protected]
Function
hread of the ICU
Phone
00385-1-4603404
Fax
00385-1-4603255
Website
http://tkojetko.irb.hr/znanstvenikDetalji.php?sifznan=2888
Research Team Objectives
Main Fields
Anaesthesiology
Therapeutics
Internal Medicine
infectious endocarditis - outcome, risk factors, echocardiography, treatment sepsis - pathogenesis,
appropriate treatment, blood stream infections in elderly severe pneumonia - outcome, mechanical
ventIlation, antimicrobial treatment, therapeutic response nosocomial infections - incidence, NI in
elderly ICU patients antimicrobial therapy - evaluation of response in severe patients, timing central
nervous system infections - outcome, treatment endocarditis - outcome, treatment, central nervous
system complications pneumonia - outcome, mechanical ventilation,
Partners/Interests
researcher
Anaesthesiology
Internal Medicine
intensivist, interested in antimicrobial treatment, availability to measure respiratory parameters in
mechanically ventilated patients
Outcome of mechanically ventilated patients with severe community-acquired pneumonia Evaluation
of therapeutic response
Institution/University
Name of Institution/University
Hospital for Infectious Diseases
Number of Employees
550
Number of Researchers
25
Country
Croatia
Postal Adress
mirogojska 8
10000 Zagreb
Anatomy
Research Team
Research Team Name
Center for Bone Morphology
Individual Researcher
Yes
Research Team Leader Name
Professor Ana Marusic
Research Team Members
Team Member 0
Resarch Assistant Katerina Zrinski Petrovic [[email protected]]
Team Member 1
Doctoral Fellow Ana Vujaklija [[email protected]]
Team Member 2
Research Assistant Sanja Ivcevic [[email protected]]
Team Member 3
Doctoral Fellow Anita Lukic [[email protected]]
Team Member 4
Doctoral Fellow Mihael Rudeš [[email protected]]
Team Member 5
Doctoral Fellow Daniela Salopek [[email protected]]
Team Member 6
Postdoctoral Fellow Natasa Kovacic [[email protected]]
Team Member 7
Postdoctoral Fellow Ivan Kresimir Lukic [[email protected]]
Team Member 8
Assistant Professor Vedran Katavic [[email protected]]
Team Member 9
Assistant Professor Danka Grcevic [[email protected]]
Research Team Projects
Project Name
Interactions Between Bone and the Immune System: A Role
for Fas-mediated Apoptosis
Project Leader
Professor Ana Marusic [[email protected]]
Project Funding Agency
Wellcome Trust
Project Budget
200000 €
Project Start Date
2004-04-01
Project End Date
2007-04-01
Project Partners
Professor Peter Croucher, UK, University of Sheffield, Medical
School, Bone Biology Group
Project Summary
The bone and the immune systems are developmentally and functionally related. Abnormal regulation
of these systems can lead to the development of disorders characterised by increased bone loss,
including osteoporosis. An important determinant of bone loss is the balance between osteoclastic
bone resorption and bone formation by osteoblasts. One process that can regulate this balance is
apoptosis. Although apoptosis has been established as an important regulator of normal and
pathological bone turnover, the molecular mechanisms mediating apoptosis in bone cells are not well
understood. Cells of the immune system are ideally placed to contribute to this process. They can be
found closely associated with the cells of bone and they also express molecules know to induce
apoptosis. One family of molecules that may play a critical role is the tumour necrosis factor family.
One member of this family, Fas-ligand (FasL), a trimeric type II membrane protein that binds to its
receptor, Fas, induces apoptosis in target cells. The role of this system in bone is poorly understood.
Osteoblasts may express Fas and undergo FasL mediated apoptosis in vitro, although the effect on
osteoclasts is less clear. In preliminary studies we have demonstrated that mice with a mutation in
the gene encoding FasL (gld, generalized lymphoproliferative disorder mice) have increased bone
mineral density. This is the result of an intrinsic disturbance in osteoblast apoptosis, as well as
increased production of osteoprotegerin, a TNF receptor family member responsible for inhibiting
osteoclastic bone resorption. The aim of the proposed study is to establish the role of FasL in
regulating bone cell apoptosis. These will include establishing a. whether osteoblasts and osteoclasts
express Fas and FasL, b. whether FasL can induced apoptosis of osteoblasts and osteoclasts, c.
whether immune cells can promote bone cell apoptosis and whether this is mediated by FasL, d.
whether gld mice have abnormalities in bone formation, e. whether Fas-ko mice, which have a gene
deletion for Fas, also have increased bone density, f. whether FasL regulates osteoblast and
osteoclast apoptosis in vivo. These studies will improve our understanding of the role of Fas/FasL in
bone and their role in bone cell apoptosis. This may lead to the identification of new therapeutic
targets for regulating bone mass.
Project Website
www.wellcome.ac.uk/
Anatomy
Research Team Projects
Project Name
Molecular interrelations between bone and immune system
Project Leader
Professor Ana Marusic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2002-08-01
Project End Date
2005-05-31
Project Partners
Project Summary
Close interdependence of the bone and immune system is well known, but their cellular and molecular
interactions in vivo have been poorly defined. Published reports give contradictory results on the role
of immune cells in bone metabolism in vivo, but also call for an integrative approach to both systems.
To test the hypothesis that immune cells are involved in the regulation of bone metabolism in vivo
and vice versa, the proposed research will investigate the bone phenotype of transgenic and mutant
mice with disturbances in the immune system, as well as indicators of bone metabolism in human
counterparts of experimental immunodeficiencies. The research will focus on the mice with
generalized lymphoproliferative disorder, which have a mutation in the Fas/Fas ligand apoptotic
system (gld and lpr mutation and Fas gene knockout). The bone phenotype will be assessed by bone
densitometry, microCT and classical histomorphometry, and the immunological phenotype by flow
citometry and sorting. We will assess bone metabolism in vivo in three experimental models: 1)
endochondral osteoinduction by BMP-2, 2) bone marrow ablation, and 3) ovariectomy-induced
estrogen depletion. The activity of bone cells in these models will be monitored at the tissue, cellular
and gene expression levels for cytokines and bone-specific molecules, using the tools of flow
cytometry and sorting, ex vivo culture, and quantitation of gene expression. Interdependence of bone
and immune systems in these mice will be investigated in bone marrow transfer experiments, where
we will characterize the bone and immune phenotype of the generated chimeras. Three
mutant/transgenic mouse lines will be used (gld and lpr mutation and Fas gene knockout) to validate
the observed phenomena and investigated if different components of the Fas system have different
roles in regulation of bone metabolism. In parallel, we will investigate bone metabolism in the human
counterpart of the gld/lpr disorder, Sjoegren’s syndrome. The experiments outlined in the project
proposal should contribute to the understanding of interactions between the bone and immune
system and proved a unified approach to the two systems. The results of the research would
particularly contribute to better understanding and possible therapeutic interventions in different
immunological and hematological disorders.
Project Website
www.mef.hr
Research Team Projects
Project Name
Development of a diagnostic procedure of quantitative PCR
for measuring cytokines in small samples
Project Leader
Professor Ana Marusic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2004-05-01
Project End Date
2006-04-30
Project Partners
The aim of the project is precommercial development of a diagnostic test for detecting the expression
of cytokines in small samples, using the quantitative PCR.
Project Website
www.mef.hr
Contact Person
Name
Professor Ana Marusic
Email
[email protected]
Function
Professor of Anatomy; Editor in Chief, Croatian Medical Journal
Phone
38514566846
Anatomy
Fax
38514590222
Website
www.cmj.hr
Research Team Objectives
Main Fields
Anatomy
Biology
Immunology and Immunohaematology
The research group from the Laboratory for Molecular Immunology at the Zagreb University School of
Medicine studies bone phenotype in different disturbances of the immune system. Bone and immune
system have not only a close anatomical but also very important functional relationship, sharing the
same cell precursors and regulatory cytokines. In our work we use in vivo models because the threedimensional nature of the cells interactions is only preserved in vivo. This we accomplish by studying
static and dynamic bone morphometry, thus assessing the volume of trabecular bone and the rate of
new bone apposition on the bone surfaces. Our laboratory is equipped with a light and fluorescence
microscope coupled to the Osteomeasure, specific histomorphometry software for bone morphometry
according to current standards. To study the cellular and molecular mechanism of cellular interactions
in the bone-bone marrow environment, we can isolate specific bone cell precursors and grow them ex
vivo. Cell development and function is assessed at several levels: 1) by classical histology (staining of
osteoblasts by alkaline phosphatase activity, staining of osteoclast for acid phosphatase activity and
assesing activitiy by resorption of bone mineral in vitro), 2) immunohistochemistry, 3) flow cytometry
of both bone and immune cells, 4) gene expression at the level of mRNA (quantitative PCR), 5) gene
expression at the protein level (Western blot and ELISA). As the full developmental potential of bone
cells and their interactions are possible only in vivo, we alos use in vivo experimental models which
replicate bone differentiation during embryonic and fetal development: 1) new bone induction at extra
skeletal sites by recombinant bone morphogenetic protein-2, which reproduces endochondral bone
development, and 2) osteogenic reparation after mechanical bone ablation, which reproduces
membranous bone development without a cartilaginous intermediate. As bone cells are sensitive to
radiation, classical experiments with bone marrow transplantation and creation of allogeneic chimeras
is not a good experimental model to study interactions between the bone and bone marrow cells, so
we have developed the parabiotic model in which joint circulation of blood cells may affect the bone
system.
Partners/Interests
researcher
industrial partner
Anatomy
Biology
Immunology and Immunohaematology
generation and analysis of transgenic mice, particularly the bone and immune phenotype
characterisation of bone phenotypes of transgenic mice, specifically with disturbances in the immune
system
Institution/University
Name of Institution/University
Zagreb University Sof Medicine
Number of Employees
600
Number of Researchers
400
Country
Croatia
Postal Adress
Salata 3
10000 Zagreb
Anatomy
Research Team
Research Team Name
reproductive biology group
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Davor Jezek
Research Team Members
Team Member 0
M.D. Gordan Grahovac [[email protected]]
Team Member 1
M.Sc., Ph.D. Sanja Vujisic [[email protected]]
Team Member 2
M.Sc. Feodora Stipoljev [[email protected]]
Team Member 3
Prof. Dr. Ruzica Pezerovic-Panijan [[email protected]]
Team Member 4
M.D. Berivoj Miskovic [[email protected]]
Team Member 5
Prof. Dr. Visnja Milavec-Puretic [[email protected]]
Team Member 6
Prof. Dr. Marina Kos [[email protected]]
Team Member 7
M.D., M. Sc. Duska Markov-Glavas [[email protected]]
Team Member 8
Prof. Dr. Gordana Juric-Lekic [[email protected]]
Team Member 9
Prof. Dr. Miro Kasum [[email protected]]
Team Member 10
Prof. Dr. Grbesa Djurdjica [[email protected]]
Team Member 11
M.D., M. Sc. Trpimir Goluza [[email protected]]
Team Member 12
Ph.D. Ozren Gamulin [[email protected]]
Team Member 13
M.D. Romana Dmitrovic [[email protected]]
Team Member 14
M.D., Ph.D. Ervina Bilic [[email protected]]
Team Member 15
M.D., Ph.D. Ernest Bilic [[email protected]]
Team Member 16
M.D., Ph.D. Tomislav Banek [[email protected]]
Team Member 17
Prof. Dr. Ljerka Banek [[email protected]]
Team Member 18
M. Sc. Maja Balarin [[email protected]]
Research Team Projects
Project Name
Investigations on the male sex gland
Project Leader
Prof. Dr. Davor Jezek [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2002-07-01
Project End Date
2006-07-01
Project Partners
Germany/University of Hamburg/Dept. of Andrology;
Austria/University of Innsbruck/Dept. of Pathology;
Slovenia/University of Ljubljana/Veterinary Faculty
Project Summary
The human testis is composed of seminiferous tubules and the interstitial tissue. Within the interstitial
tissue, numerous macrophages, Leydig (interstitial) cells and blood vessels are situated. Recent
investigations on the morphology and histophysiology of the rodent testis demonstrated specific
cellular junctions between macrophages and Leydig cells. Macrophages influence steroidogenesis and
testosterone production in Leydig cells via paracrine factors (i.e. lipophilic factor 25hydroxycholesterol, interleukins 1 and 6 and tumour necrosis factor alpha /TNF-alpha/). In addition,
Leydig cells affect neighbouring macrophages by secreting paracrine steroidogenic, proopiomelanocorticoid and other factors. Besides macrophages, interstitial blood vessels influence the
testosterone serum levels. These vessels demonstrate rhytmic contractions called "vasomotion", thus
changing the absorption of testosterone. AIMS & HYPOTHESIS. The emphasis of the study would be
on the development, normal histophysiology and pathology of macrophages, Leydig cells and blood
vessels. In patients with non-obstructive azoospermia, an extensive structural change of the
testicular interstitial tissue takes place. This change includes an increase in the number of
macrophages, distrubance of steroidogenesis within Leydig cells and the consequent pathology of
blood vessels. The above mentioned changes of the interstitial tissue would be compared with the
status of spermatogenesis in infertile patients. EXPECTED RESULTS. The proposed project would
Anatomy
reveal new data on the development, normal histophysiology and pathology of macrophages, Leydig
cells and blood vessels within the human testis. Moreover, the diagnostic histopathological evaluation
of the testicular biopsy would be imporved by: 1. introduction of new markers for macrophages; 2.
estimation of the steroidogenesis and testosterone production in Leydig cells; 3. electron-microscopic
analysis of the testis interstitial tissue. Parallel comparison of the pathology in the interstitium and
within seminiferous tubules (analysis of the preserved number of sperms and late spermatids) would
gain prognostic parameters for the therapy of the patients with non-obstructive azoospermia.
METHODS: 1. Immunohistochemistry; 2. Electron microscopy; 3. Morphometry (stereology).
IMPORTANCE OF THE PROJECT. New data on the testis interstitium and their application in the human
reproductive medicine.
Project Website
Research Team Projects
Project Name
Histophysiological regulation of human Leydig cells
Project Leader
Prof. Dr. Davor Jezek [[email protected]]
Project Funding Agency
Slovenian Agency for Scientific Projects
Project Budget
2000 €
Project Start Date
2003-01-01
Project End Date
2006-01-01
Project Partners
Slovenia/University of Ljubljana/Veterinary Faculty
Project Summary
Morphological and physiological analyses revealed many differences between sexes beside the
primary (sexual glands) and secondary (external sexual characteristics) sexual dimorphisms. Many
studies have shown differences between male and female brains, where most differences were
described in preoptic area, although some reports also suggest sexual dimorphisms in regions such as
hypothalamus and hippocampus. Beside brain, several studies described sex dependent differences in
salivary glands. In rodents, there are differences in composition of saliva as well as in the morphology
of several salivary glands. Glandula loewenthali was thought to be a part of parotid gland until
recently due to vicinity of both glands. However, glandula loewenthali is clearly separated from
surrounding tissue. Macroscopically, the gland is dark color and in shape of lens. In rats, sex
differences can be observed macroscopically, as gland has bigger volume in males. With aging special
cells with many lipid droplets and large amount of smooth endoplasmatic reticulum appear in this
gland. Due to their similarity to the cells from harder’s gland they are called harder’s cells.
Interestingly, these cells appear only in male rate glandula loewenthali ut not in females. Castration
causes reduction in number of this cells while ovariectomy does not affect the gland. Even bigger
differences appear in glandula loewenthali at castration of male mice. In mice, both activitiy and
amount of acinar cells is reduced after castration, and some acinar tissue is replaced with
interstitium. However, in spite of some reports of sexual dimorphisms in this gland in rodents, there
are no clear morphological description (especially at electron microscopy level) of these glands in
mice and rats, and it is not known the mechanism causing sexual dimorphism. It is not known
whether sex dependent differences are completely hormone dependent or are also influenced by
genetic background. Steroidogenic factor 1 (SF-1) is a member of nuclear receptor family, essential
for normal development and function of endocrine organs. SF-1 knockout mice are born without
gonads and adrenals and because gonads in these mice regress several days before steroidogenesis
starts, they are never exposed to endogenous steroid hormones. Therefore, SF-1 KO mice represent
an excellent and unique model to study sexual differentiation in the absence of steroid hormones.
Due to adrenal insufficiency, these mice die few days after birth, but we developed a method of
adrenal transplantation that enables us to breed these mice into adulthood. In our laboratory, studies
looking for sex differences in behaviors and brain gene expression are already underway. Under
current proposal, we would like to extend these studies by examining the influences of hormones and
genetic background on sexual dimorphism in salivary glands.
Project Website
Research Team Projects
Project Name
Reinke's crystals in healthy and infertile men
Project Leader
Prof. Dr. Davor Jezek [[email protected]]
Project Funding Agency
Austrian Ministry of Science and Reasearch
Project Budget
2000 €
Project Start Date
2006-01-01
Anatomy
Project End Date
2007-12-31
Project Partners
Austria/University of Vienna/Institute of Materials Physics
Project Summary
Infertility in men and women has been recognized as a major health problem within the European
Union (EU) as well as in the neighbouring European countries. It is estimated that infertility affects
15% of all couples trying to conceive. Epidemiological studies have pointed out that half of infertility
problems could be related to the male factor. In many cases (30-70%) the cause for male infertility is
not known or not clarified enough (Oehninger, 2001; Sharma et al. 2004). All the above-mentioned
data go for the both, Austrian and Croat population. Health systems in Europe are thus confronted
with two major problems: the rising number of infertile couples and the rising cost for their
treatment. Therefore, it is necessary to reveal factors that contribute to infertility and work out the
strategy for the prevention of such health risks. Our project focuses on the male infertility and the
role of Reinke's crystals (located in Leydig cells of the male gonad), since these structures are found
in normal and infertile adult human testis. Studies on Reinke’s crystals are rare (approx. 20
references in Medline) and their function remains unclear. Of particular interest is comparison of
Reinke's crystals in healthy and infertile men with idiopathic non-obstructive azoospermia. Infertile
men who have non-obstructive azoospermia (no spermatozoa in their ejaculate) are especially
difficult to treat and, therefore, represent population of patients that needs special care and attention
of andrologist/urologist and other medical personal (Hauser et al., 1998; Ježek et al., 1998; Amer et
al., 2000; Salihu and Aliyu, 2003).
Project Website
Contact Person
Name
Prof. Dr. Davor Jezek
Email
[email protected]
Function
Associate Professor, Assistant to Dean
Phone
+38514590251
Fax
+38514590251
Website
www.mef.hr
Research Team Objectives
Main Fields
Anatomy
Cytology
Pathology
Connective tissue is an important constituent of the male and female reproductive tract. Therefore,
the main object of investigation in this survey would be an interstitial tissue of the testis and
connective tissue (stroma) of the chorionic villi of the placenta. In addition, the influence of sex
glands on some anatomically distant organs and tissues will be investigated. Hypothesis on which the
survey is based upon is threefold: a) interstitial compartment of the testis significantly influences the
normal development, architecture and function of the whole organ (i.e. testis) as well as the
occurrence of the male infertility; b) connective tissue/stroma of the chorionic villi significantly
influences the normal development, architecture and function of the whole organ (i.e. placenta) as
well as the occurrence of infertility in females (emphasis on the intrauterine growth restriction); c)
removal of sex glands (gonadectomy) significantly affects the architecture and function of
anatomically distant organs. a) Testis. The aim of this part of the survey would be to get data on the
development of macrophages, Leydig cells and blood vessels in the human foetus. In addition, to
check if the disturbed architecture and function of these cells/structures contributes to the infertility
in man. b) Placenta. The main goal of this part of the project would be to investigate the development
and the interaction between so-called Hofbauer cells and blood vessels of the chorionic villi. The
normal placentas and placentas form intrauterine growth restriction cases would be compared. c)
Sexual dimorphism of the extraorbital lachrymal gland of the rat. This part of the project would deal
with the influence of the sex glands (i.e. their removal) on the extraorbital lachrymal gland of the rat.
Special emphasis would be given on the changes of the interstitium of the gland. Methods. The
following methods will be employed: immunohistochemistry, transmission electron microscopy,
transmission electron microscopy with immunogold particles, morphometric (stereological) analysis
and molecular biology methods. The expected results would provide valuable data on the
development of interstitial tissue of the testis and connective tissue of the chorionic villi. Moreover,
the role of macrophages, Leydig cells and blood vessels in the occurrence of infertility in males and
females would be elucidated. The assessed data could be very useful in human reproduction. In
addition, the role of sex glands in the formation of some tertiary sex characteristics would be clarified.
Anatomy
Partners/Interests
researcher
industrial partner
Pathology
Obstetrics and Gynaecology
Public health services
multiarray DNA analysis; proteomics, genital infections diagnostics
Infertility in South-East Europe
Institution/University
Name of Institution/University
Medical School University of Zagreb
Number of Employees
600
Number of Researchers
400
Country
Croatia
Postal Adress
Salata 3b
10000 Zagreb
Bacteriology
Research Team
Research Team Name
Chlamydia
Individual Researcher
No
Research Team Leader Name
Mr., PhD Radovan Hojs
Research Team Members
Team Member 0
Mr., PhD Artur Pahor []
Team Member 1
Mr., MD, PhD Ivan Krajnc []
Team Member 2
Mr. MD Aleš Goropevšek []
Team Member 3
Mrs., PhD Avrelija Cencic []
Contact Person
Name
Mr., PhD Radovan Hojs
Email
[email protected]
Function
Head of Department of Medical didactics
Phone
+ 386 2 234 56 01
Fax
+ 386 2 234 56 00
Website
www.f.uni-mb.si
Research Team Objectives
Main Fields
Bacteriology
Chlamydia
Partners/Interests
researcher
industrial partner
Suitable
Bacteriology
1. CHLAMIDIAL INFECTIONS and INFLAMMATION – EXPLOITATION OF THE HOST –EVOLVING TO
INFLAMMATORY DISEASES LIKE ATHEROSCLEROSIS, (RHEUMATOID ARTHRITIS AND ASTHMA)
Inflammation is now recognised as being pivotal in the pathogenesis of atherosclerosis. Lesions of
atherosclerosis consist of blood-borne inflammatory and immune cells that represent important part
of atheroma, the remainder being vascular endothelial and smooth muscle cells. They interact with a
series of specific cellular and molecular responses that can be described as an inflammatory disease.
Cholesterol deposits, found in the arterial wall before any fatty streak-like lesion occurred, can
activate complement and initiate cascade that produces powerful chemotactic stimuli for monocytes
to enter the forming lesion. Chlamydia(C) pneumoniae is an obligate microbe that commonly causes
respiratory infection and is able to infect human smooth muscle cells, arterial endothelial cells and
macrophages. It has a unique life cycle – on exposure to a variety of stimuli including antibiotics
microbe can convert to a long, inactive intracellular phase in the form of so called cryptic bodies. In
this phase Chlamydia is not replicating and is resistant to mode of action of most antibiotics. C
pneumoniae contains heat shock protein 60 like sub-units that coud cause inflammatory injury via
autoimmune reaction thus indirectly trigger atherogenesis To study early C. pneumoniae infection and
infection in correlation with the onset of inflammatory response, we will study the interaction between
C. pneumoniae and respiratory tract cells using an in vitro co-culture model. The integrity of a human
lung bronchial epithelial (CRL-9482) cell layer and the impact of C. pneumoniae attachment to its
status will be determined by measuring transepithelial resistance (TER) and transepithelial potential
of the cell monolayer growing on microporous membranes. The translocation of bacteria will be
monitored by detection of the microorganisms in media and in macrophages growing in the lower
chamber. Cencic group will also study whether IFN-gamma, a proinflammatory cytokine, when added
to the basolateral side, will disrupt the tight epithelium and facilitate penetration of C. pneumoniae
through the respiratory epithelium. The induction of inflammatory signals in epithelial cells and
macrophages (NF-kB, cytokines, iNOS) after C. pneumoniae infection will be determined by ELISA,
mRNA-expression arrays and/or RT-PCR. Finally, the cross-talk between C. pneumoniae, lung
epithelium and macrophages will be determined to shed light on the early events of infection when C.
pneumoniae enters the host respiratory tract. Upon infection, a chlamydial activity protects infected
cells against apoptotic stimuli and this could lead to chronic-active and persistent infections during
human chlamydial diseases. Häcker group has recently shown that specific degradation of proapoptotic host proteins (so-called BH3-only proteins) is responsible for this activity. Simultaneously,
chlamydial infection has a propensity to induce atypical death of the infected cell. At this stage, it is
Bacteriology
completely unclear which bacterial effector proteins cause the opposing biological effects (inhibition of
apoptosis and induction of cell death). However, chlamydial proteins secreted into the cell e.g. via the
chlamydial type III secretion system are the most promising candidates. To screen for inhibitors of
apoptosis, pooled chlamydial plasmid libraries will be transfected into respiratory epithelial cells.
Transfected cells will be subjected to UV-irradiation to induce apoptosis. After 24 h, surviving cells will
be harvested, plasmids will be re-isolated, amplified in bacteria and used in a second round of
transfection. The procedure will be repeated until cells transfected with individual plasmids can be
recovered. Focusing on the bacterial components identified by Hegemann and Subtil groups WP x,
expression plasmids will be transfected, and transfected cells will be compared for their UV-resistance
with vector-transfected controls. To identify death-inducing proteins, HeLa cells will be transfected
with a marker of transfection (GFP) and pools (approximately 20 plasmids) of library samples. When
a reduction of GFP-positive cells is seen by flow cytometry, the pools will further be split to identify
the active protein. For candidate secreted proteins (identified in WP x), a similar approach will be
taken. Upon identification of such chlamydial proteins, their interaction with the host cell’s
components and the precise mechanism of their interference with cell death will be defined. The
subsequent efforts will be directed at the pharmacological inhibition of these bacterial targets (WP x
drug development) and the interference with the corresponding host cell’s targets by RNAi (WP x).
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Biochemistry
Research Team
Research Team Name
Biochemistry
Individual Researcher
No
Research Team Leader Name
Mrs., PhD Avrelija Cencic
Research Team Members
Team Member 0
Mr., PhD Dimitri Komiotis []
Team Member 1
Mr., PhD Costa Antonakis []
Team Member 2
Mr. MD Aleš Goropevšek []
Contact Person
Name
Mrs., PhD Avrelija Cencic
Email
[email protected]
Function
Head of Department of Biochemistry
Phone
+ 386 2 2505 828
Fax
+ 386 2 23 45 600
Website
http://www.mf.uni-mb.si/
Research Team Objectives
Main Fields
Biochemistry
Other allied sciences
Partners/Interests
researcher
industrial partner
Suitable
Other allied sciences
1. INTERFERONS AND NEWLY SYNTHESISED AMINO-KETONUCLEOSIDES AS ANTIVIRAL AND
ANTICANCER AGENTS Interferons (IFNs) are proteins or glycoproteins belonging to a wide family of
cytokines. IFNs exert a broad spectrum of biological activities, like elicitation of an "antiviral state" in
target cells, that is a transient resistance to infection by numerous viruses, moreover interferons,
namely IFN-gamma were shown as strong inhibitors of cancerogenesis and regulator of
immunomodulatory activities. Two main types of IFNs have been described, that share no sequence
homology : type I IFNs (a, b, t, d, w), include IFNs that are produced mainly in response to a variety
of viruses. Type II IFNs include an unique member - IFN-g, produced in adult mammals by activated
T lymphocytes and NK cells, that has a multipotential role in immune response. Nucleoside analogues
display a wide range of biological activities as anti-tumor, anti-viral and chemotherapeutic agents. In
recent years a number of 2´, 3´-unsaturated nucleoside analogues, such as D-2´-3´-didehydro-2´3´-dideoxy-5-fluorocytidine (D-d4FC) and its L-enantiomer (L-Fd4C), have been identified as anti-HIV
agents. Cytosine and 5-fluorocytosine derivatives from the L series display potent HBV (EC50 = 0.002
and 0.004 ìÌ, respectively)7 as well as anti-HIV-1 activities without significant cytotoxicity. More
recently, the description of the structure-activity relationships of L-3´-fluoro-2´-3´-unsaturated
nucleosides, showed potent activity in the cytosine and 5-fluorocytosine derivatives (EC50 = 0.089
and 0.018 ìÌ, respectively). Furthermore, cytosine nucleoside analogues, such as 1-(2-deoxy-2methylene-â-D-erythro-pentofuranosyl)
cytosine
(DMDC),
2´-deoxy-2´-2´-difluorocytidine
(gemcitabine), and 1-(2-C-cyano-2-deoxy-â-D-arabino-pentofuranosyl)cytosine (CNDAC), have been
developed as potent antitumor agents, which are effective not only on leukemias and lymphomas, but
also on a wide variety of solid tumors in vitro as well as in vivo. It is the purpose of our work to study
the potential effects of interferon gamma and newly synthesized nucleoside analogues as potent
inhibitors of tumor cells and to try to elucidate the mechanism behind the action.
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Biochemistry
Research Team
Research Team Name
Clinic of Dentistry Institute of Biochemistry
Individual Researcher
No
Research Team Leader Name
Mr.sci dr.stom. Ana Pejcic
Research Team Members
Team Member 0
teaching ass Radmila Obradovic [-]
Team Member 1
Associate Prof Zoran Pesic [[email protected]]
Team Member 2
teaching ass Aleksandar Petrovic [-]
Team Member 3
associate Prof Tatjana Cvetkovic [[email protected]]
Team Member 4
Professor Ljiljana Kesic [-]
Team Member 5
Associate Prof Jelenka Nikolic [[email protected]]
Team Member 6
Professor Vesna Zivkovic [[email protected]]
Team Member 7
teaching ass Ana Pejcic [[email protected]]
Team Member 8
Professor Branislava Mirkovic [[email protected]]
Team Member 9
Professor Draginja Kojovic [-]
Contact Person
Name
Mr.sci dr.stom. Ana Pejcic
Email
[email protected]
Function
Phone
9938118512500
Fax
Website
Research Team Objectives
Main Fields
Biochemistry
Dentristry
biochemical investigation of gingival fluid, enzymes and saliva immunohistochemical investigation of
gingival inflammation with MIB-1, Ki67 antigen, p27, p21 and p53
Partners/Interests
researcher
Biochemistry
Dentristry
Other allied sciences
researchers of biochemical science researchers of pathological science researchers of periodontal
disease
Periodontal disease is a major problem for the oral health worker. It is the result of the accumulation
of dental plaque at the marginal gingivae leading to inflammation of the periodontal tissues.
Periodontal disease is prevalent in most human population and results in significant morbidity, with
premature tooth loss in severely affected individuals. Much research has been carried out into the
epidemiology, aetiology, prevention, and clinical management of perioontal disease, and this has
resulted in a significant increase in our understanding of the condition. Chronic periodontitis is defined
as plaque-induced inflammation of the periodontal tissues which has resulte in destruction of the
periodontal ligament, loss of crestal alveolar bone, and apical migration of the epithelial attachment.
Bacterial plaque is the primary aetiological actor in periodontal disease and the disease will not occur
in the absence of plaque. Most local secondary factors are mechanical plaque traps, promoting plaque
accumulation at specific sites. Periodontal disease is result of the interaction of bacteria and the
factors derived from plaque with the host tissues. The destructive and protective mechanisms which
operate in periodontal disease are normally considered to be in equilibrium, resulting in a stable or
quiescent lesion. Bursts of periodontal breakdown may occur when this balance is upet, either
because of an increase in destructive factors, or because of a decrease in the effectiveness of
protective mechanisms. The ability to identify the mechanisms likely to result in periodontal
breakdown might have important clinical implicatins. Firstly, it might be possibleto identify and target
high risk groups of patients for special care and management, and, secondly, it might be possible to
determine more accurately the efficacy of treatment procedures. The reasons for the occurrence of
periodontal destruction, the factors which may determine disease susceptibility, and the reasons for
Biochemistry
disase progression at different sites in the same patient are some of the most important questions in
periodontology today. Before discussing the processes and mechanisms involved in periodontal
disease, it is important to establish a sound undestanding of the structure of the normal
periodontium. The gingiva provides attachment between the oral mucous memrane and the dental
hard tissues and protects the underlying periodontal tissues from invasion by the bacteria present in
the oral cavity. In health this is at the amelo-cemental junction, but apical migration of the junctional
epithelium occurs in disease. Oral epithelium is stratified squamous epithelium and, like other similar
epithelia, is composed of keratinocytes and non-keratinocytes or clear cells. Cell devision normally
occurs only in the basal cell layet and the cells which arise from these divisions move
graduallythrough the epithelium towards the surface, where they are shed. These changes are
accompanied by the synthesis of a number of cell products, the most conspicuous of which is keratin,
which packs the cells in the keratinized layer and contributes to the mechanical toughness of the
superficial layers. This process of maturation is termed differentation. The non-keratinocyte
population of epithelium comprises melanocytes, lymphocytes, Langerhans cells, and Melker cells.
The keratinocytes in oral epithelium are continually shed from the surface, and in health these are
replaced by an equal number of successors arising from cell division in the basal cell layers. Epithelial
turnover is influenced by factors which affect the rate of cell division, the maturation and movement
of cells through the prickle cell layers, and the rate of desquamation. Keratinocytes in the prickle cell
layer producecd substances called cytokines. These are proteins or glycoproteins which are produced
by a variety of cell types and which regulate the growth and differentationb of other cells. Cytokines
usually act localy, although some have a systemic action. Epithelial turnover appears to be affected
by at least three cytokines: epidermal growth factor (EGF), transforming growth factor alpha (TGFÜ),
and transforming growth factor beta(TGFâ). The process of re-epitelization that leads to healing of
oral mucosal wounds results from a carefully orchestrated proliferation and migration of epithelial
cells. Cell migration occurs through a combination of basal cell migration and sliding of the epithelial
cell mass above the migrating basal cells. Cellular proliferation is a fundamental biological process
coordinating with other processes, tissues homeostasis and repair, require stringent control. The
ideas of this projects are: • To elucidate the molecular basis of the lack of cellular proliferation in the
migrating epithelial gingivae during the re-epithelization of oral mucosal wounds; • To elucidate the
expression of cell-cycle regulators critical for G1-phase progression and S-phase analysed
immunohistochemically; • To compare normal human mucosa across gingival epithelia migrating with
marker Ki67; • To study cell kinetics of gingival, sulcular and junctional epithelia in periodontitis; • To
investigate expression of p21 and possible correlation between this parameter and clinicopathologic
features, p53 accumulation and the proliferation-associated marker Ki67; • To investigate the effects
of lipopolysaharides on keratinocyte growth factor (KGF) and expression levels of KGF in normal and
inflamed gingival tissue.
Institution/University
Name of Institution/University
Medical Faculty - group for dentistry
Number of Employees
154
Number of Researchers
25
Country
Serbia and Montenegro
Postal Adress
Bld. Z. Djindjica 81
18000 Nis
Biochemistry
Research Team
Research Team Name
Department for Molecular Biology
Individual Researcher
No
Research Team Leader Name
Dr Goran Poznanovic
Research Team Members
Team Member 0
Sci. Consultant Miodrag Petrovic [[email protected]]
Team Member 1
Sci. Consultant Goran Poznanovic [[email protected]]
Team Member 2
Res. Assistant Aleksandra Uskokovic [[email protected]]
Team Member 3
Res. Scientist Melita Vidakovic [[email protected]]
Team Member 4
Res. Scientist Mirjana Mihailovic [[email protected]]
Team Member 5
Sci. Consultant Svetlana Ivanovic-Matic [[email protected]]
Team Member 6
Res. Scientist Vesna Martinovic [[email protected]]
Team Member 7
Sr. Res. Scientist Ilijana Grigorov [[email protected]]
Team Member 8
Res. Assistant Nevena Grdovic [[email protected]]
Team Member 9
Res. Scientist Svetlana Dinic [[email protected]]
Team Member 10
Sci. Consultant Desanka Bogojevic [[email protected]]
Team Member 11
Jr. Res. Assistant Jelena Arambasic
[[email protected]]
Research Team Projects
Project Name
Acute and chronic stress: homeostaic regulatory
mechanisms in the acute radiation syndrome and diabetes
Project Leader
Science Consultant Goran Poznanovic [[email protected]]
Project Funding Agency
Min. Sci. Environ. Protect. Rep. Serbia
Project Budget
97180 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
(Project realized in: Serbia / Institute for Biological Research /
Department of Molecular Biology)
Project Summary
Examiniation of the adaptive mechanisms to acute homeostatic challenges after exposure of the rat to
(i) ionizing radiation and (ii) during a sustained homeostatic disturbance in streptozotocin-induced
diabetes. The project is focused on (i) the acute-phase (AP) proteins: alpha1-acid glycoprotein (AGP),
alpha2-macroglobulin (MG) and haptoglobin (Hp), circulatory proteins whose synthesis increase
significantly during the AP response, an essential manifestation of the adaptive process, and on (ii)
the mechanisms that regulate poly(ADP-ribosyl) polymerase (PARP-1) activity in homeostatic
processes that are executed in response to oxidant stress during the acute radiation syndrome (ARS)
and prolonged inflammation during diabetes. Work on the project is expected to (i) elucidate the
control of the AP response in ARS and diabetes by characterizing the molecular signals and molecular
regulators of AP protein gene transcription, (ii) establish the potential protective roles of the
individual AP proteins during the ARS and diabetes, and to (iii) clarify the molecular mechanisms that
control PARP-1 activity as a precondition of the second phase of research that would examine the
molecular events in the cell after PARP-1 inhibition. This is of particular importance in light of the
presumed role that PARP-1 inhibitors have in therapy of the pathophysiological changes occurring in
diabetes and other types of inflammation.
Project Website
Project Name
Acute and chronic stress: homeostaic regulatory
mechanisms in the acute radiation syndrome and diabetes
Project Leader
Science Consultant Goran Poznanovic [[email protected]]
Project Funding Agency
Min. Sci. Environ. Protect. Rep. Serbia
Project Budget
97180 €
Project Start Date
2006-01-01
Biochemistry
Project End Date
2010-12-31
Project Partners
(Project realized in: Serbia / Institute for Biological Research /
Department of Molecular Biology)
Project Summary
Examiniation of the adaptive mechanisms to acute homeostatic challenges after exposure of the rat to
(i) ionizing radiation and (ii) during a sustained homeostatic disturbance in streptozotocin-induced
diabetes. The project is focused on (i) the acute-phase (AP) proteins: alpha1-acid glycoprotein (AGP),
alpha2-macroglobulin (MG) and haptoglobin (Hp), circulatory proteins whose synthesis increase
significantly during the AP response, an essential manifestation of the adaptive process, and on (ii)
the mechanisms that regulate poly(ADP-ribosyl) polymerase (PARP-1) activity in homeostatic
processes that are executed in response to oxidant stress during the acute radiation syndrome (ARS)
and prolonged inflammation during diabetes. Work on the project is expected to (i) elucidate the
control of the AP response in ARS and diabetes by characterizing the molecular signals and molecular
regulators of AP protein gene transcription, (ii) establish the potential protective roles of the
individual AP proteins during the ARS and diabetes, and to (iii) clarify the molecular mechanisms that
control PARP-1 activity as a precondition of the second phase of research that would examine the
molecular events in the cell after PARP-1 inhibition. This is of particular importance in light of the
presumed role that PARP-1 inhibitors have in therapy of the pathophysiological changes occurring in
diabetes and other types of inflammation.
Project Website
Contact Person
Name
Dr Goran Poznanovic
Email
[email protected]
Function
Science Consultant
Phone
+381.11.2078342
Fax
+381.11.2761433
Website
http://www.ibiss.bg.ac.yu/
Research Team Objectives
Main Fields
Biochemistry
Biology
Physiology
Acute and chronic stress: homeostaic regulatory mechanisms in the acute radiation syndrome and
diabetes. Examination of the adaptive mechanisms to acute homeostatic challenges after exposure of
the rat to ionizing radiation and during a sustained homeostatic disturbance in streptozotocin-induced
diabetes. The project is focused on (i) the acute-phase (AP) proteins: alpha1-acid glycoprotein (AGP),
alpha2-macroglobulin (MG) and haptoglobin (Hp), circulatory proteins whose synthesis increase
significantly during the AP response, an essential manifestation of the adaptive process, and on (ii)
the mechanisms that regulate poly(ADP-ribosyl) polymerase (PARP-1) activity in homeostatic
processes that are executed in response to oxidant stress during the acute radiation syndrome (ARS)
and prolonged inflammation during diabetes. Work on the project is expected to (i) elucidate the
control of the AP response in ARS and diabetes by characterizing the molecular signals and molecular
regulators of AP protein gene transcription, (ii) establish the potential protective roles of the
individual AP proteins during the ARS and diabetes, and to (iii) clarify the molecular mechanisms that
control PARP-1 activity as a precondition of the second phase of research that would examine the
molecular events in the cell after PARP-1 inhibition. In the first stage of research the mechanisms of
regulation of PARP-1 functioning under basal conditions, during DNA repair, apoptosis and necrosis
will be studied by examining the dynamic interactions of PARP-1 with the nuclear matrix, its potential
protein binding partners (A/C lamins, transcription factor NF-kB that plays a key role in the inducible
expression of genes mediating proinflammatory effects), and matrix association regions on DNA. The
research is expected to extend to the examination of the molecular events in the aftermath of PARP-1
inhibition.
Partners/Interests
researcher
Biology
Biochemistry
Physiology
Established primary cardiocyte cell culture, molecular biological and cytological methods of
characterization of different types of cell death. Proteomics.
Initial degradation of chromatin into high-molecular weight DNA fragments during apoptosis reflects
Biochemistry
the periodicity of chromatin organization into nuclear matrix-attached loops. In the paper: Grdović, N.
& Poznanović, G.: Characterization of an Mg2+-dependent endonucleolytic activity of the rat
hepatocyte nuclear matrix. Comp. Biochem. Physiol. Part B. (2003) 136:495-504, we put forward the
hypothesis that this pattern of DNA cleavage is also a result of the localization of an endonuclease on
the nuclear matrix. Namely, we observed an endonucleolytic activity of the isolated rat hepatocyte
nuclear matrix. It was Mg2+-dependent, with an optimal activity at pH 7.2 in the absence of either
Na+ or K+. It was fully active in the presence of Zn2+ and capable of introducing single-strand
breaks into plasmid DNA. It did not display a sequence-specific activity. A 23 kD DNA nuclease that
was principally localized on the rat hepatocyte nuclear matrix was detected. The enzyme shared the
biochemical requirements with the nuclear matrix endonucleolytic activity, thus we proposed that p23
could be responsible for the endonucleolytic activity of the nuclear matrix. In view of its properties
and preferential localization on the nuclear matrix, the endonuclease described herein could be a
possible candidate that brings about initial DNA cleavage during apoptosis. To investigate the nature
of the interaction of p23 with the nuclear matrix, the nuclear matrix was prepared using different
procedures and examined for the presence/absence of the enzyme by activity gel analysis. Treatment
of isolated nuclei with sodium tetrathionate (NaTT), a sulfhydryl-cross-linking agent, led to the
complete recovery of p23 in the nuclear matrix, whereas incubation of nuclei with dithithreitol (DTT),
a sulfhydryl-reducing agent, led to its complete solubilization and resulting absence from the nuclear
matrix. Exposure of the isolated nuclear matrix to DTT in high-ionic strength buffer, a procedure that
promotes the solubilization of the internal nuclear matrix, caused the nearly complete solubilization of
p23. It was concluded that disulfide bonds play an essential role in the association of p23 with the
nuclear matrix and that p23 is mostly localized in the nuclear matrix interior. (Grdović, N., Vidaković,
M. & Poznanović, G. Binding of a 23 kD endonuclease to the rat liver nuclear matrix. (2005) Gen.
Physiol. Biophys. 24: 99-111). Diabetes is associated with persistent oxidative stress which
eventually leads to the development of diabetic endothelial and myocardial dysfunction. The
pathomechanism envisions that the reactive oxyradicals that are persistently generated in the
diabetic state cause considerable DNA strand breakage. The subsequent activation of cellular DNA
repair processes leads to PARP-1 overactivity; the concomitant ATP and NAD+ consumption results in
a cytotoxic failure of energy metabolism that leads to cardiomyocyte death that promotes the
development of cardiomyopathy. Recently, we established the presence of significant p23 activity in
nuclear matrices prepared from cardiocyte nuclei that were isolated from control rats and from rats
during streptozotocin-induced diabetes. We wish to characterize the role of the nuclear matrixassociated endonuclease p23 in the signaling pathway(s) in oxyradical-induced cardiomyocyte death
during streptozotocin-induced diabetes.
Institution/University
Name of Institution/University
Institute for Biological Research
Number of Employees
212
Number of Researchers
179
Country
Serbia and Montenegro
Postal Adress
Despot Stephen Boulevard 142
11060 Belgrade
Biochemistry
Research Team
Research Team Name
Department of Biochemistry
Individual Researcher
No
Research Team Leader Name
PhD Anita Markotic
Research Team Members
Team Member 0
assistant professor Irena Drmic Hofman []
Team Member 1
assistant Vedrana Cikes Culic [[email protected]]
Team Member 2
assistant professor Anita Markotić [[email protected]]
Team Member 3
Professor Maja Pavela-Vrancic []
Team Member 4
BSc Sandra Dujic Bilusic []
Research Team Projects
Project Name
Optimization of factors that influnce liver regeneration
Project Leader
Assistant Professor Anita Markotic [[email protected]]
Project Funding Agency
Ministry of Science, Edication and Sports CRO
Project Budget
25000.00 €
Project Start Date
2001-12-14
Project End Date
2003-12-14
Project Partners
Germany/University of Muenster/Institute for Medical Physics and
Biophysics
Project Summary
The aim of the project was to find optimal protocol of hyperbaric oxygenation (HBO) on rat liver. Rats
were sacrificed 54 h after 15% hepatectomy, liver and body weights were measured, and serum
alanine transaminase (ALT) and aspartate transaminase (AST) activity and albumin levels were
determined. The lipid peroxide level, as indicated by malondialdehyde production in the remnant liver
was measured, and liver sections were analyzed by light microscopy. Five groups of 10 rats in each
group were studied. The preHBO and pre-hyperbaric pressure (preHB) groups were treated before
partial hepatectomy with 100% O2 and 21% O2, respectively, at 202,650 pascals, daily for 3 days
(45 min/ day). The control group was not treated before partial hepatectomy and recovered under
normal ambient conditions after the procedure. Groups postHBO and postHB were treated after
partial hepatectomy with HBO and HB, respectively, three times (45 min/day). The preHBO group
presented a significant increase in the initiation of the regeneration process of the liver 54 h
postoperatively. The liver/body weight ratio was 0.0618 ± 0.0084 in the preHBO compared to 0.0517
± 0016 g/g in the control animals (P = 0.016). In addition, the preHBO group showed significant
better liver function (evaluated by the lowest serum ALT and AST activities, P = 0.002 and P = 0.008,
respectively) and showed a significant decrease in serum albumin levels compared to control (P <
0.001). Liver lipid peroxide concentration was lowest in the preHBO group (P < 0.001 vs control and
postHBO group) and light microscopy revealed that the composition of liver lobules in the preHBO
group was the closest to normal histological features. These results suggest that HBO pretreatment
was beneficial for rat liver regeneration after partial hepatectomy.
Project Website
www.mefst.hr
Project Name
Expression of glycosphingolipids in regenerated rat liver
Project Leader
Assistant Professor Anita Markotic [[email protected]]
Project Funding Agency
Ministry of Science, Edication and Sports CRO
Project Budget
17000.00 €
Project Start Date
2002-08-22
Project End Date
2006-05-31
Project Partners
Germany/University of Muenster/Institute for Medical Physics and
Biophysics
Project Summary
Gangliosides from livers of weanling rats were analysed after 15% partial hepatectomy (PH) and
different pre- and post-operative hyberbaric oxygenation (pre- and postHBO). Neu5Ac was the
predominant ganglioside-derived sialic acid (>85%) compared to Neu5Gc. Almost identical low total
sialic acid content (Neu5Ac+Neu5Gc) of the control and operated nonHBO animals opposed a 6.4 to
Biochemistry
7.6 fold increase in pre- and postHBO animals (69.26 and 81.64 pmol per mg wet weight,
respectively). NanoESI-QTOF mass spectrometry combined with HPTLC immunostaining revealed
GM3(Neu5Ac) and GM3(Neu5Gc) as major gangliosides, correlating with the respective sialic acid
concentrations. Minor neolacto-series gangliosides were enhanced in preHBO and postHBO, but GM1core gangliosides only in preHBO rats. GM2 and GalNAc-GM1b were clearly detectable in oxygenated
rats compared to traces in the control and nonHBO animals. These results point at a functional role of
gangliosides in liver growth regulation and reconstitution after PH combined with pre- and postoperative HBO treatment.
Project Website
www.mefst.hr
Contact Person
Name
PhD Anita Markotic
Email
[email protected]
Function
assistant professor at Split University Medical School
Phone
0038521557938
Fax
0038521557625
Website
www.mefst.hr
Research Team Objectives
Main Fields
Biochemistry
Genetics
Immunology and Immunohaematology
1. Liver regeneration after partial hepatectomy. 2. Glycosphingolipid (GSL)-phenotype in relation to
genotype of different leucocyte populations (neutrophiles, monocytes and T lymphocytes) after
exercise. 3. GSL expression in relation to genotype in cerebrospinal fluid of children with neurological
disorders.
Partners/Interests
researcher
Biochemistry
Genetics
Immunology and Immunohaematology
1. Ability to work with hepatic cell culture or 2. Flow cytometry
1. Immunohistochemical analyses of glycosphingolipid (GSL) expression in regenerated rat liver. 2.
GSL-phenotype and genotype of different leucocyte populations (neutrophiles, monocytes and T
lymphocytes) after exercise. 3. GSL-phenotype and genotype of children with neurological disorders.
Institution/University
Name of Institution/University
Split University Medical School
Number of Employees
90
Number of Researchers
65
Country
Croatia
Postal Adress
Soltanska 2
21000 Split
Biochemistry
Research Team
Research Team Name
Department of Biochemistry - Stress Research Group
Individual Researcher
No
Research Team Leader Name
PhD Gordana Matic
Research Team Members
Team Member 0
BSc Tatjana Perisic [[email protected]]
Team Member 1
BSc Ivana Elakovic [[email protected]]
Team Member 2
BSc Sanja Manitasevic [[email protected]]
Team Member 3
MSc Jelena Brkljacic [[email protected]]
Team Member 4
BSc Danijela Vojnovic Milutinovic [[email protected]]
Team Member 5
PhD Jadranka Dundjerski [[email protected]]
Team Member 6
PhD Gordana Matic [[email protected]]
Research Team Projects
Project Name
Glucocorticoid receptor and heat shock proteins expression
and function in pathophysiological states and stress
Project Leader
PhD Gordana Matic [[email protected]]
Project Funding Agency
Ministry for Science of Serbia
Project Budget
70000 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Serbia / University of Belgrade / Faculty of Medicine, Institute of
Endocrinology, Diabetes and Metabolic Disease
Project Summary
Glucocorticoids have a broad array of life sustaining functions and play an important role in the
therapy of many diseases. Hence, changes in tissue sensitivity to glucocorticoids, either in the
direction of resistance or hypersensitivity, may be associated with and may influence the course and
treatment of many pathological states. One of the most important determinants of tissue
responsiveness to glucocorticoids is the level and function of the glucocorticoid receptor (GR), an
intracellular protein acting as hormone activated transcription factor. The subject of the present
research project are molecular mechanisms underlying GR structural and functional modulation. It
includes both human and animal studies, in which diverse pathological states and stressful conditions
related to changes in tissue sensitivity to glucocorticoids are exploited in order to learn more on the
mechanisms regulating GR expression and function. Special attention will be paid on the functional
significance of GR interaction with heat shock proteins closely associated with the receptor within
multiprotein heterocomplexes. The research along these lines may contribute to better understanding
of the biological principles underlying glucocorticoid hormones action and their extensive use as
therapeutic means.
Project Website
www.ibiss.bg.ac.yu/odeljenja/biohemija
Project Name
Psychobiology of posttraumatic stress disorder
Project Leader
MD, PhD Eric Vermetten [[email protected]]
Project Funding Agency
European Commission
Project Budget
1300000 €
Project Start Date
2004-10-01
Project End Date
2007-09-30
Project Partners
Netherlands / University of Utrecht / University Medical Center;
Serbia / University of Belgrade / Faculty of Medicine, Institute of
Endocrinology Diabetes and Metabolic Disease; Serbia / University
of Belgrade / Institute for Biological Research "Sinisa Stankovic";
Serbia / International Aid Network, Belgrade; Italy / University of
Bari / Specialization School of Psychiatry; Croatia / University of
Rijeka / Medical Faculty, Psychiatric Clinic; United Kingdom /
University of London / Queen Mary and Westfield College; Serbia /
Biochemistry
Military Medical Academy, Belgrade; Serbia / Vinca Institute of
Nuclear Sciences, Belgrade.
Project Summary
Post-traumatic stress disorder (PTSD) is the most common war-related psychiatric disorder occurring
among combat veterans and other people exposed to war zone stress. In addition to health problems,
this disorder causes numerous long-term socioeconomic damages. Current opinion is that the best
results in PTSD understanding, diagnosis and treatment could be achieved by integrating
psychological, biological and pharmacotherapeutical approaches. General objective of this project is to
better understand the biological basis of psychophysical profiles of PTSD patients. The study will focus
on establishing multiple correlations of different PTSD subtypes with relevant psychological,
biochemical, endocrinological, genetic, physiological and anthropometric parameters. The
psychological and biological studies in this project will be performed on five groups of subjects: a)
PTSD patients, b) subjects with PTSD in remission, c) traumatized subjects without PTSD, d) healthy
controls from Western Balkans, and e) healthy controls from Western Europe. All subjects in these
groups will be male. Additionally, the same psychological studies will be performed on five analogous
groups of female subjects. The traumatic experiences of the first three groups (of both genders) are
war-related. The main psychological instruments to be used are personality inventories and
dissociation questionnaires. Biological measurements will encompass parameters related to
hypothalamo-pituitary-adrenocortical axis (including cortisol receptor and its gene polymorphism),
anthropometry, body composition, lipid status, insulin resistance, and sleep disturbances. After an
advanced statistical analysis of the data, this study will yield new knowledge on relations between: a)
basic psychological variables and PTSD, b) biological variables and PTSD, and c) biological and basic
psychological variables in health and in PTSD. In addition, the foreseen benefits of the project
include: d) development of combined psycho-biological batteries for PTSD screening, diagnosing and
risk factors assessing, e) improvement of psychological instruments for measuring PTSD, f)
implementation of new biological markers for PTSD, g) recommendation for the improvement of
combined psycho- and pharmacotherapy of PTSD.
Project Website
www.pbptsd.org
Contact Person
Name
PhD Gordana Matic
Email
[email protected]
Function
Head of Department/Professor
Phone
+381 11 2078303; +381 63 8121713
Fax
+381 11 2761433
Website
www.ibiss.bg.ac.yu/odeljenja/biohemija
Research Team Objectives
Main Fields
Biochemistry
Genetics
Physiology
(1) Molecular mechanisms underlying cellular and organismal response to stress and stress-related
disease; (2) Stress- and disease-related alterations in glucocorticoid receptor and heat shock proteins
expression; (3) Stress- and disease-related modulation of glucocorticoid receptor and heat shock
proteins function
Partners/Interests
researcher
Biochemistry
Genetics
Physiology
Our potential partners should share similar scientific interest and should be able to conduct
complementary research.
The goal of the new project will be to gain new knowledge on the complex mechanisms regulating
biological activity of glucocortcioid receptor (GR) with the focus on the role of heat shock proteins
(Hsps) in chaperoning the receptor and modulating its basic biological functions. The results of the
research should help to assess how important are the levels of the receptor and its individual isoforms
expressed in particular cellular context for tissue sensitivity to glucocorticoids and for diversity of
tissue specific effects of these hormones. They should also help to establish correlation between GR
functional parameters and tissue responsiveness to glucocorticoids. Finally, our studies should
contribute to understanding the role of GR and Hsps in pathogenesis and clinical course of stress
related somatic and psychic disorders associated with dysfunction of these proteins. The somatic
Biochemistry
disorders of special interest are asthma, metabolic syndrome X, polycystic ovary syndrome and
diabetes, while the psychic ones are posttraumatic stress disorder and major depressive disorder.
While the molecular structure and the mechanisms of GR functioning are now well established, the
mechanisms modulating GR activity and expression, as well as those underlying diversity of tissue
specific effects of glucocorticoid hormones remain largely unknown. Among other processes, these
complex mechanisms include action of Hsps and posttranslational modifications of the receptor. They
operate at almost every step of glucocorticoids action, from binding to the receptor to
stimulation/inhibition of target genes transcription, exerting major influence on tissue sensitivity to
these hormones. In order to better understand mechanisms responsible for modulation of GR
expression and activity, in our studies we will exploit different corticosteroid related
pathophysiological and stressful conditions to follow alterations in GR expression and function. A part
of our research will be focused on human GR from peripheral blood mononuclear cells as readily
available human cells that reflect situation in immune and neural tissue. Using quantitative Western
blot technique and real time PCR, as well as standard hormone binding and tissue sensitivity assays
we will examine the levels of GR protein and mRNA, its hormone binding capacity and affinity, as well
as the expression of the two receptor isoforms in stress related diseases, including somatic and
psychic disorders mentioned above. For example, knowing that posttraumatic stress disorder and
major depressive disorder are associated with the opposite changes of hypothalamo pituitary
adrenocortical axes sensitivity to glucocorticoids, these diseases will be exploited as a suitable model
for studying modulation of GR function. At the same time, these studies will contribute to better
understanding of the role of GR in pathogenesis of the studied diseases. Human GR will also be
investigated in patients suffering from mild and severe forms of asthma, a disease almost inevitably
connected to corticosteroid treatment. Asthma is characterized by inflammation and generation of
reactive oxygen and nitrogen species in respiratory tract, and in our research will be employed for
examining posttranslational modifications of the receptor, such as oxidation of cysteine and nitration
of tyrosine residues. A part of our research concerning the effects of stressors such as hyperthermia
and heavy metals on GR structural and functional properties will be performed on rat liver and kidney
GR. Special attention will be paid on the role of heat shock proteins (Hsp90 and Hsp70) in
chaperoning the receptor and modulating its function, as well as on their modifications potentially
connected to their general role in cellular response to different stressors and in pathogenesis of
somatic diseases. For analysing functional interactions of these proteins with GR we will apply
coimmunoprecipitation and quantitative Western blot to follow stress related changes in Hsps levels in
the cell and within GR multiprotein heterocomplexes.
Institution/University
Name of Institution/University
Institute for Biological Research "Sinisa Stankovic"
Number of Employees
230
Number of Researchers
192
Country
Serbia and Montenegro
Postal Adress
Despot Stefan Boulevard 142
11000 Belgrade
Biochemistry
Research Team
Research Team Name
Institute Vinca, Laboratory for Molecular Genetics
Individual Researcher
No
Research Team Leader Name
PhD Esma Isenovic
Research Team Members
Team Member 0
Bs.Sci Jelena Velebit [[email protected]]
Team Member 1
PhD Ljiljana Markovic [[email protected]]
Team Member 2
Bs.Sci Emina Sudar [[email protected]]
Team Member 3
Mr.Sci Snezana Tepavcevic [[email protected]]
Team Member 4
Mr.Sci Mojca Vulovic [[email protected]]
Team Member 5
PhD Zorica Zakula [[email protected]]
Team Member 6
PhD Goran Koricanac [[email protected]]
Team Member 7
PhD Esma Isenovic [[email protected]]
Team Member 8
MD,PhD Biljana Putnikovic [[email protected]]
Team Member 9
PhD Milan Orlic [[email protected]]
Team Member 10
MD, MSci Zoran Gluvic [[email protected]]
Research Team Projects
Project Name
Molecular mechanisms of transduction of hormonal signals:
Biological markers of Modifications and integrations of
signaling pathways in physiological and pathophysiological
conditions.
Project Leader
PhD, Res Associate Prof Esma Isenovic [[email protected]]
Project Funding Agency
Ministry of Sciences of Republik Serbia
Project Budget
10000 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Project Summary
Diabetes mellitus is a powerful risk factor for the development of cardiovascular disease (CVD), which
is the leading cause of morbidity and mortality in persons with diabetes. Insulin (INS) and Insulin like growth factor -1 (IGF-1) induces vasorelaxation in part by enhancing nitric oxide (NO) production
and Na+, K+-ATPase (Na+pump) activation in vascular endothelial (EC) smooth muscle cells (VSMC).
Estradiol (E2) like IGF-1 and INS normally exerts vasodilatory and protective effects by increasing
vascular NO and VSMC Na+pump activity, among other mechanisms. Our preliminary studies indicate
that IGF-1 and E2 are one of the regulators of NOS and Na+ pump in vitro, and their effects are
mediated by cascade of 3 eznymes: phosphatidylinositol 3 kinase (PI3K)-cytosolic phospholipase 2
(cPLA2)-protein kinase B (Akt). As IGF-1 and E2 exert similar actions on NO and Na+pump activity in
vascular tissue, we anticipate that they act synergistically to increase NOS/ Na+pump. We
hypothesized that, when PI3K-cPLA2-Akt signaling cascade is interrupted ( i.e. INS resistance or
molecular knockout pathway) , sinergisam of the IGF-1 and E2 will be abolished. The reninangiotensin system (RAS) is important in the pathogenesis of hypertension and associated CVD.
Hypertensive patients are more likely than normotensives to develop type 2 diabetes and this
propensity may reflect resistance to INS and a reduction in the ability of INS to promote relaxation
and glucose (GLU) transport in vasculature and skeletal muscle tissue, respectively. Resistance to INS
exists in vascular as well as skeletal muscle and adipose tissue (major sites of INS-mediated GLU
disposal). Preliminary data from our laboratory, as well as others, suggest that angiotensin 2 (Ang
II), acting through its AT1 receptor (AT1R), inhibits the actions of INS in vasculature, skeletal muscle
and adipocytes via increases in RhoA activity. We seek to test the hypothesis that Ang II stimulation
of Rho A compromises vascular INS/Akt signaling and consequent NOS/Na+,K+-ATPase activation
and GLU disposal in INS resistant models of hypertension. The proposed studies will provide unique
insights into alternative pathways linking IGF-1, INS and E2 signal transduction with cardiovascular
diseases, thereby providing sites for gene and/or drug therapy.
Project Website
Biochemistry
Project Name
EU coperation Grant COST B17: “Obesity and Diabetes
mellitus in the Elderly”
Project Leader
ResAssoProfe Esma Isenovic [[email protected]]
Project Funding Agency
COST EU
Project Budget
100000 €
Project Start Date
1999-12-16
Project End Date
2005-12-15
Project Partners
Austria Professor K.h. TRAGL Austria Professor Josef PATSCH
Belgium Professor Louis HUE Cyprus Dr. Joseph KASIOS Czech
Republic Professor Jaroslav VESELY Czech Republic Dr. Bela
BENDLOVA Denmark Dr. Christine REYNET Denmark Dr. Aase
HANDBERG France Professor Emmanuel VAN OBBERGHEN Germany
Professor Juergen ECKEL Greece Dr. Effie TSILIBARI Greece
Professor George DIMITRIADIS Hungary Dr. György GYERMENDY
Ireland Dr. John NOLAN Israel Professor Shlomo SASSON Israel
Professor Nava BASHAN Italy Dr. Francesco GIORGINO Lithuania
Dr. Vaidotas URBANAVICIUS Lithuania Dr. Valentinas
MATULEVICIUS Netherlands Dr. J.a. MAASSEN Norway Professor
Arild Chr. RUSTAN Norway Dr. Jorgen JENSEN Poland Professor
Aldona DEMBINSKA-KIEC Romania Professor Dan CHETA Romania
Dr. Maya SIMIONESCU Serbia and Montenegro Dr. Esma ISENOVIC
Slovak Republic Title Elena SEBOKOVA Slovak Republic Dr. Iwar
KLIMES Slovenia Professor Robert ZOREC Slovenia Professor
Marjan KORDAS Spain Dr. Isabel VARELA-NIETO Spain Professor
Margarita LORENZO Spain Dr. Antonio ZORZANO Sweden Professor
Juleen ZIERATH Sweden Professor Jan ERIKSSON Switzerland Dr.
Markus NIESSEN Switzerland Professor Jean-Louis CARPENTIER
United Kingdom Professor Kenneth SIDDLE
Project Summary
Diabetes mellitus of aged people is a considerable health burden of western societies. At present no
widely established screening method is available to detect the persons who are predisposed to this
condition. Due to our incomplete knowledge of the molecular mechanisms leading to NIDDM we have
few means to influence the onset and development of this diverse disease. The ongoing research of
candidate genes as well as various differential screening methods are coordinated with this Action.
Various steps of insulin action are studied starting from the insulin receptor and following insulin
action until it reaches the cell nucleus. There is special emphasis on the investigation of pathological
changes of the molecular mechanism of insulin action in insulin resistance, obesity and NIDDM of the
aged people. This research activity leads to the development of new drug-candidates for the curingeasing of the consequences of the disease.
Project Website
http://www.webio.hu/workshop/cost
Project Name
European Commission Framework Programme: Equal Project
(EC4): Multi-National External Quality Assay (EQA)
Programmes in Clinical Molecular Diagnostics based on
Performance and Interpretation of PCR assay including
dissemination and training, supported by the European
Community within the FP6
Project Leader
ResAssProf particicapnt: Esma Isenovic [[email protected]]
Project Funding Agency
EU
Project Budget
739000 €
Project Start Date
2004-01-01
Project End Date
0000-00-00
Project Partners
Prof. M. Pazzagli Department of Clinical Physiopathology University
of Florence, Italy www.dfc.unifi.it Dr. R. Jansen European
Communities Confederation of Clinical Chemistry and Laboratory
Medicine (EC4) Geldrop, The Netherlands www.ec-4.org Prof. M.
Neumaier Faculty for Clinical Medicine, University Hospital
Mannheim of the University of Heidelberg, Germany www.ma.uniheidelberg.de/inst/ikc Prof. J.C. Libeer Scientific Institute of Public
Biochemistry
Health, Clinical Biology Department Brussels, Belgium
www.eqalm.org A. Martens, MD ZiekenhuisGroep Twente Almelo,
The Netherlands www.zgt.nl Dr. S. Ramsden UKNEQAS for
Molecular Genetics Manchester, UK www.ukneqas-molgen.org.uk
Dr. D. Taruscio Istituto Superiore di Sanità Rome, Italy www.iss.it
Prof. V. Palicka Institute for Clinical Biochemistry and Diagnostics
Medical Faculty and Teaching Hospital Hradec Kralove, Czech
Republic www.fnhk.cz
Project Summary
Molecular biology based technologies and Genomic studies have opened new perspectives in
diagnosis, prognosis and treatment of Clinical Medicine. Molecular Diagnostics are now available for
the Clinical Chemistry and Medical Genetic laboratories but the level of standardisation of these tests
in terms of instrumentation, reagents and procedures is, at the moment, lower than the usual
Laboratory Medicine tests. External quality assurance (EQA) programmes for molecular diagnostics
have been developed in order to safeguard quality of these assays, but up till now EQA programs
addressed to specific DNA/RNA targets remain limited to a few tests. The Partners of the Project,
Coordinators of EQA programmes of molecular diagnostics already implemented at national or
international level, propose a panel of EQA programmes on Performance and Interpretation of PCR
based assay methods. These methodological EQA programmes will be available to laboratories
performing Molecular Diagnostics on a voluntary basis for monitoring performances of technical
procedures common to most of these methods. Specifically developed questionnaires and procedures
of data analysis will be implemented for monitoring efficacy and appreciation of these EQA
programmes.Diffusion of these EQA programmes to other countries will be supported through
international organisms of the area of Laboratory Medicine, such as EC4. Finally specific training
courses will be organised by the partners for the participants of this survey in order to improve both
technical and interpretation skills required for the correct use of these molecular diagnostic tests. It is
expected that integration between EQA programmes addressed to methodological aspects and to
specific analytes, with appropriate international training activities will improve the quality of molecular
diagnostics.
Project Website
http://www.ec-4.org/
Project Name
“Insulin-like Growth Factor-1 (IGF-1) Regulation of Nitric
Oxide Synthase (NOS) and Sodium Pump in Type 1 Diabetes
“
Project Leader
ResAssoProf Esma Isenovic [[email protected]]
Project Funding Agency
MNT and EDGE
Project Budget
1750 €
Project Start Date
2006-01-01
Project End Date
3007-12-31
Project Partners
CNRS UMR, Univ P& M Curie, Paris, France ( Dr. P. Marche)
Project Summary
Insulin-like growth factor-1 (IGF-1), acts in an autocrine/paracrine fashion on vascular smooth
muscle cells (VSMC) and cardiomyocytes. IGF-1 is known to diminish vascular tone by modulating
cation and nitric oxide (NO) metabolism IGF-1 increases vascular inducible NO synthase (iNOS) and
Na+, K+-ATPase (sodium pump) expression and activity via a phoshatidylinositol 3-kinase
(PI3K)/protein kinase B (Akt) signaling in VSMC. Cytosolic phospholipase 2 (cPLA2) is known to
regulate Na+ pump activity. Vascular cPLA2 activity is increased by IGF-1 treatment. We hypothesize
that in type 1 diabetes the decreased ability of IGF-1 to stimulate iNOS and Na+ pump activity is due
to altered PI3K-cPLA2-Akt signaling cascade, which modulates iNOS and sodium pump
activity/expression.
Project Website
http://www.egide.asso.fr; http://www.mntr.sr.
Project Name
“Response to insulin on cellular level”
Project Leader
ResAssProf Esma Isenovic [[email protected]]
Project Funding Agency
Ministry of Sciences Serbia and Slovenia
Project Budget
2500 €
Project Start Date
2006-01-01
Project End Date
2007-12-31
Project Partners
University of Ljubljana, Slovenia (Prof. R. Zorec)
Biochemistry
Project Summary
Objective: INS resistance (decreased tissue INS action) is characterized by one or more postbinding
defects that mediate INS stimulation of GLUT. Reduced INS sensitivity is a characteristic feature of
various pathological conditions such as type 2 diabetes and hypertension. Hypertensive patients are
more likely than normotensives to develop type 2 diabetes and this propensity may reflect resistance
to INS and a reduction in the ability of INS to promote relaxation and GLUT in adipose tissue and
skeletal muscle cells, respectively. Recent studies suggest that these action of INS is mediated
through the activation of phosphatidylinositol-3-kinase (PI3K) and the downstream, protein kinase B
(Akt) signaling cascade. This signaling pathway increases GLUT in adipocytes and skeletal muscle
tissues. Thus, functional alterations in these INS signaling pathways are likely to play an important
role in these pathologies. In this regard, there is emerging evidence that angiotensin II (Ang II)
interferes with this signaling, resulting in a state of resistance to INS mediated GLUT; however, the
role of intermediary signaling molecules are unclear. Hypothesis: Although we intend to further
explore the actions of INS in cultured skeletal muscle cells, the major thrust of our proposal seeks to
test our primary hypothesis: INS regulates in skeletal muscle cells GLU metabolism, in part, by
increasing the mobilization of the INS sensitive- GLUT-4 to the PM by exocytosis. Specifically, we
hypothesize that Ang II attenuates the ability of INS to increase GLU permeability of skeletal muscle
cells by interfering with signaling through the PI3K/Akt in skeletal muscle cells. Description of the
project: Since, exocytosis of vesicles is associated with an increase in plasma membrane surface area
that can be monitored by electrophysiological measurements of membrane capacitance; we will
employ confocal microscopy to observe the changes in PM area after stimulation with INS and/or Ang
II. The proposed studies employing single cultured skeletal muscle cells will be used to answer the
following key questions. 1) Do INS and Ang II induce changes in PM area and mobilization of GLUT4
to the PM? 2) Whether PI3K/Akt signaling pathway is involved in mediating interactions between Ang
II and INS in regulation of GLUT and GLUT 4 mobilizations to the PM?
Project Website
www.mnt.sr.gov.yu
Contact Person
Name
PhD Esma Isenovic
Email
[email protected]
Function
Principal Investigator, Associate Res Professor
Phone
+381647029163
Fax
+381112159978
Website
www.vin.bg.ac.yu
Research Team Objectives
Main Fields
Biochemistry
Internal Medicine
Public health services
Investigations focused on characterization of the effects of different hormones such as insulin, IGF-1,
Ang II and estradiol in order to address critical questions regarding the impact of disease (i.e.,
diabetes, hypertension). Studies were carried out in primary cultures of vascular smooth muscle cells,
rat aortic endothelial cells and adult cardiomyocytes. In vivo studies were carried out in different rat
models (Zucker obese, mREN etc)
Partners/Interests
researcher
Biology
Internal Medicine
Public health services
Well established scientists with interest in the field of metabolic syndrome.
Insulin resistance (IR) is usually characterized by a) higher levels of insulin (INS) while fasting and
post-glucose (GLU) loading, and b) decreased tissue responsiveness to INS-driven clearance of GLU
from the bloodstream. INS regulates blood GLU levels primarily by stimulating uptake of GLU by
muscle and fat cells and curbing hepatic GLU output. GLU is transported via a family of GLU
transporter proteins (GLUTs), with GLUT4 being the major INS-responsive isoform in muscle and fat.
Intracellular GLUT4 is translocated to the cell surface in response to INS by a mechanism requiring
activation of phosphatidyl-inositol (PI)3-kinase (P13K) and protein kinase B (Akt). IR seems to be a
common feature and a possible contributing factor of several frequent health problems, including type
2 diabetes mellitus (DMT2), sleep-related breathing disorder (SRBD) and obesity. IR induced by a
high-fat (HF) diet and associated obesity are major risk factors for DM and cardiovascular diseases.
Epidemiologic studies have delineated strong links between obesity, dyslipidemia, IR or diabetes. But
the question remains: The significance of adipose tissue for INS sensitivity became a major focus
Biochemistry
following the finding that this tissue is an active endocrine organ, secreting several factors (e.g.,
leptin and adiponectin) that can affect whole-body INS sensitivity. Adipocytes increase adiponectin
synthesis in vitro in response to INS, which has a lesser effect in adipocytes from obese animals,
although in vivo INS appears to decrease adiponectin levels. Infusion of INS and GLU to maintain
euglycemia in humans leads to increased circulating leptin levels after 3 h. In rats with streptozotocin
(STZ)-induced diabetes, leptin levels fall but can be restored by INS in a dose-dependent fashion,
with prevention of the hyperphagia characteristic of uncontrolled diabetes. In cultured adipocytes,
INS increases leptin production, which can be blocked with 2-deoxyglucose in a dose-dependent
fashion, suggesting that GLU uptake is required for an effect on leptin.. Understanding the complex
interaction between IR, and obesity may lead to more effective, better tolerated treatments for DMT2
and IR The mechanism by which INS and adipokines such as leptin and adionectin might be
associated remains poorly understood, and will be be further addressed in my future work .
Institution/University
Name of Institution/University
Institute Vinca
Number of Employees
850
Number of Researchers
450
Country
Serbia and Montenegro
Postal Adress
Mike Alasa 15
11000 Beograd
Biochemistry
Research Team
Research Team Name
Laboratory for basic research in Urological Oncology
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Tatjana Simic
Research Team Members
Team Member 0
Dr. Dejan Dragicevic [[email protected]]
Team Member 1
Dr. Marija Opacic [[email protected]]
Team Member 2
Dr. Marija Pljesa-Ercegovac [[email protected]]
Team Member 3
Dr. Ana Savic-Radojevic [[email protected]]
Team Member 4
Prof. Dr. Jasmina Mimic-Oka [[email protected]]
Team Member 5
Prof.Dr. Tatjana Simic [[email protected]]
Research Team Projects
Project Name
Role of glutathione S-transferases in urinary tract
carcinomas
Project Leader
Prof.Dr. Tatjana Simic
[[email protected];[email protected]]
Project Funding Agency
Ministry of Science and technology of Serbia
Project Budget
36000 €
Project Start Date
2006-01-01
Project End Date
2007-12-31
Project Partners
Serbia, University of Belgrade, Institute of Biochemistry Serbia,
University Clinical Centre, Institute of Urology and nephrology
Project Summary
Urinary tract organs have an important role in excretion of endogenous and exogenous metabolites.
Therefore, kidney tissue and epithelium of renal pelvis, ureters and urinary bladder are exposed to
various xenobiotics, including carcinogens. Exposure to potential carcinogens is among etiological
factors for renal cell carcinoma and transitional cell carcinoma of urinary bladder. Renal cell
carcinoma is very resistant, while transitional cell carcinoma of urinary bladder exibit high recurrence
and multifocality. Glutathione S-transferases (GSTs) may play an important role in occurrence,
progression and resistance of these tumors. Cytosolic GSTs are a superfamily of enzymes (classes
alpha, mu, pi and theta), which protect normal cells by catalyzing conjugation reactions between
electrophylic compounds and glutathione. In tumor cells, GSTs are responsible for resistance to
antitumor drugs. Certain GSTs also have regulatory role in regulation of mitogen-activated protein
kinases (MAPK), involved in induction of apoptosis. To discern the role of GSTs in urinary tract
carcinomas, whithin this project we aimed to (1) identfy unknown and known GST isoenzymes and
clarify their role in the occurrence and resistance of these tumors (2) establish whether relationship
between GST expression at protein level and tumor progression exists and if any of GST isoenzymes
might be used as tumor marker (3) determine whether interactions between GST and MAPK exist and
clarify regulatory role of GST isoenzymes in regulation of apoptosis.
Contact Person
Name
Prof. Dr. Tatjana Simic
Email
[email protected]
Function
Associated Professor, Institute of Biochemistry
Phone
+381 11 2645750
Fax
+381 11 2645750
Website
http://www.med.bg.ac.yu
Research Team Objectives
Main Fields
Biochemistry
The laboratory for Basic research in Urological Oncology belongs to the Institute of Biochemistry,
Medical Faculty, Belgrade. Our scientific group is composed by one full Professor (Jasmina MimicOka), one associated professor (Tatjana Simic), one post Doctorate Researcher (Ana Savic Radojevic)
and two PhD Candidates (Marija Pljesa-Ercegovac and Marija Opacic). Clinical urolgist Dr Dejan
Biochemistry
Dragicevic, PhD, engaged as teaching assistant at our Faculty, is also member of the group. Our
studies have been funded by national Minsitry of Science and technology. Members of the team have
an expertise on the fields of enzymology, oxidative stress and malignant phenotype of urinary tract
carcinomas. The major aim of the team is to elucidate the role of glutathione S-transferases (GST) in
urinary tract tumors. GSTs are xenobiotic-metabolyzing enzymes which play a role in response to
exposure to carcinogenic chemicals as well as in chemoresistance and apoptosis of tumor cells.
Therefore, we aim to perform systematic functional investigation of different GST classes in
transitional cell carcinoma of urinary bladder and upper urothelial tumors, by using an array of
substrates of differential specificity and Western blot. Corresponding non-tumor tissue will also be
studied. Glutathione (GSH) level and the activities of key enzymes involved in its synthesis,
replenishment and degradation, together with the activity of antioxidant enzymes will be measured.
The scientific excellence of the group is proven by the bibliographic entries. Major equipment
•Equipment for purification and characterization of proteins (enzymes) including affinity
chromatography, chromatofocusing, isoelectric chromatofocusing, electrophoresis, Western blot,
immunoassays •Equipment for spectrophotometric measurement of the activities of glutathione Stransferases, antioxidant enzymes (GSTs, gamma-glutamyl transpeptidase, gamma-glutamyl cysteine
synthetase, glutathione reductase, glutathione peroxidase and superoxide dismutase) and
quantification of free radical activity (isoprostane analysis, carbonyl groups, malondialdehyde etc.).
Partners/Interests
researcher
Biochemistry
Toxicology
We search for partners which have an expertise on the fields of MAP kinase pathways, with specific
emphasis on JNK pathway and its role in apoptosis. Partners that have excellence on the study of
protein:protein interactions by means of confocal microscopy or other methods would be also
welcome. We also search partners engaged in investigations of malignant phenotype of transitional
cell carcinoma, especially those that study proteome and metabolome of transitional cell carcinoma
and bladder tumor cell lines. It would be of interest to find partners involved in the research of agents
that modulate GST activity/expression in in vitro conditions.
Bladder cancer has been cited to result from the neoplastic lesion with environmental and/or
occupational factors identified as causatives. Transitional cell carcinoma (TCC) is the most common
type of bladder cancer. Most of the bladder cancer patients die from the invasive, metastatic TCC that
shows resistance to chemotherapy. Glutathione S-transferase (GST) superfamily members have been
shown to be involved in chemotherapy resistance of many human tumors. Our studies on the
expression of GST in uroepithelium and transitional cell carcinoma of urinary bladder have shown
significant upregulation of GSTP1-1 in tumor cells. Recently, a new antiapoptotic role for GSTP1-1 has
been described. Namely, GSTP1-1 potently and selectively inhibits activation of c-jun protein by its
upstream kinase, c-jun NH2-terminal kinase (JNK). The JNK belongs to the family of stress kinases
and has been shown to be required for the induction of apoptosis by DNA-damaging agents. Recently,
an inhibition of JNK activity by GSTP1-1, which was reversed by polymerization induced by oxidative
stress, has been reported in mouse fibroblast cell lines. This newly identified regulatory activity of
GSTP1-1 is strongly inhibited by a group of agents (7-nitro-2, 1, 3-benzoxadiazole derivatives). The
cytotoxic mechanism of these inhibitors has been carefully investigated in leukemic CCRF-CEM and
K562 cell lines. Western blot and immunoprecipitation analyzes have shown that 6-(7-nitro-2, 1, 3benzoxadiazol-4-ylthio) hexanol promotes in both cell lines the dissociation of the GSTP1-1 from a
complex with JNK. This process triggers a reactive oxygen species (ROS)-independent activation of
the JNK-mediated pathway that results in a typical process of apoptosis. In the present study we aim
to examine whether GSTP1-1 have antiapoptotic role in the transitional cell carcinoma of urinary
bladder. Besides we will also examine whether such role is medited by GSTP1-1:c-jun NH2-terminal
kinase interaction and how it depends on cellular redox balance. Therefore it is of particular interest
to determine whether such interaction exists in tumor specimens as well as in transitional cell
carcinoma cell lines such as 5637, BFTC905, HT1197, J82, SCaBER, T24, TSGH-8301, and TCCSUP
T24. In case we prove antiapoptotic role of GSTP1-1 in bladder tumor cell lines, cytotoxic activities of
new 7-nitro-2, 1, 3-benzoxadiazole derivatives will be tested as promising anticancer agents.
Institution/University
Name of Institution/University
University of Belgrade, Medical Faculty
Number of Employees
861
Number of Researchers
861
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgrade
Biochemistry
Research Team
Research Team Name
Molecular biology of mental disorders
Individual Researcher
No
Research Team Leader Name
BSc, PhD Dorotea Mück-Seler
Research Team Members
Team Member 0
PhD student Martina Dezeljin [[email protected]]
Team Member 1
PhD student Maja Mustapic [[email protected]]
Team Member 2
PhD Nela Pivac [[email protected]]
Contact Person
Name
BSc, PhD Dorotea Mück-Seler
Email
[email protected]
Function
senior scientist
Phone
385-1-4571 207
Fax
385-1-4561 010
Website
www.irb.hr
Research Team Objectives
Main Fields
Biochemistry
Genetics
Psychiatry
The investigation of the peripheral biochemical markers in the etiology of schizophrenia, depression
and Alzheimer's disease. Genes and mental disorders. Pharmacogenomics and proteomics
Partners/Interests
researcher
Psychiatry
Pharmacology
Genetics
Experienced psychiatrist or neurologist
Biochemical and genetical markers, in the prediction of the treatment response in depression
(unipolar, bipolar), schizophrenia
Institution/University
Name of Institution/University
R.Boskovic Institute
Number of Employees
700
Number of Researchers
400
Country
Croatia
Postal Adress
Bijenicka 54
10000 Zagreb
Biochemistry
Research Team
Research Team Name
Molecular Medicine Unit, Institute of Biochemistry, Faculty
of Medicine, University of Belgrade
Individual Researcher
No
Research Team Leader Name
Dr. Ivanka Markovic
Research Team Members
Team Member 0
Dr. Tatjana Radnic [[email protected]]
Team Member 1
Dr. Sonja Misirlic Dencic [[email protected]]
Team Member 2
Dr. Olivera Vuckovic [[email protected]]
Team Member 3
Dr. Aleksandra Isakovic [[email protected]]
Contact Person
Name
Dr. Ivanka Markovic
Email
[email protected]
Function
Associate Professor
Phone
+381-11-2683-841
Fax
+381-11-2682-953
Website
www.med.bg.ac.yu
Research Team Objectives
Main Fields
Biochemistry
Clinical chemistry
Neurology
The main fileds of interest are the role of nucleosides and their analogues in idifferent cellular
processes; furthermore, we are interested in the biochemical and molecular mechanism of
neurodegenerative disorders. Existing facilities at Institute of Biochemistry offer an opportunity to
investigate the biochemical and structural bases of molecular and cellular processes in a variety of
mammalian cells, using live-cell investigation (cell culture, protein expression, cell cycle, intracellular
signalling, etc.), gene expression, use of fluorochromes for identification and quantification of
different markers involved in cellular processes, and a number of other techniques useful in studying
and understanding cellular and molecular mechanisms that regulate cells’ responses to different
stimuli.
Partners/Interests
researcher
industrial partner
Biology
Clinical chemistry
Neurology
The partner should have similar research interests (mainly but not restricted to biochemical and
molecular aspects of neurodegeneration) together with research methodology and know-how that is
compatible to ones we use. We would also appreciate if a potential partner would have experience
and expertise in molecular biology and genetic manipulations (creation of constructs, work on knockout animal models etc) or if our potential partner would be interested in testing of biological activity
and metabolic fate of different synthetic compounds.
Our research interests in the following period are mainly focused on different aspects of functioning of
the cells of the CNS (astrocytes, choroid plexus epithelium, endothelial cells and neurons) in
physiological and pathological conditions, using in vivo and in vitro models, as well as how the
interactions between the cells change upon action of different noxious influences (i.e. molecular and
biochemical aspects of degeneration and cell death). The main focus of future research will be work
on primary cell cultures (rat astrocyte and neuronal culture, choroid plexus epithelium, brain
andothelial cells) and biochemical and molecular investigation of the cells originating from the clinical
samples (function of monocyte-derived macrophages and dermal fibroblast in patients from
neurodegenerative disorders). The research activities would employ a wide range of approaches,
including molecular and cell biology, biochemistry, multi-color FACS analysis, cell imaging using
confocal microscopy etc. Furthermore, the existing research methodology and know-how at our Unit
enables us to investigate the transfer of compounds across the barriers of the brain (BBB and bloodCSF barrier) using both in vivo and in vitro models (mechanism and kinetics of transport).
Biochemistry
Institution/University
Name of Institution/University
University of Belgrade, Faculty of Medicine
Number of Employees
1400
Number of Researchers
850
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgrade
Biochemistry
Research Team
Research Team Name
Research team for biophysical chemistry and enzymology
Individual Researcher
No
Research Team Leader Name
Dr Vesna Vasic
Research Team Members
Team Member 0
Dr Danijela Krstic [[email protected]]
Team Member 1
Bsc Mirjana Colovic [[email protected]]
Team Member 2
Msc Katarina Krinulovic [[email protected]]
Team Member 3
Bsc Ana Vujacic [[email protected]]
Team Member 4
Msc Jasmina Savic [[email protected]]
Team Member 5
Msc Tatjana Momic [[email protected]]
Contact Person
Name
Dr Vesna Vasic
Email
[email protected]
Function
senior researcher
Phone
+381 11 2453 967
Fax
+381 11 444 7207
Website
vin.bg.ac.yu
Research Team Objectives
Main Fields
Biochemistry
Toxicology
Information Technology, Statistics, Documentation
The main field of the interest is the study of the enzyme inhibitors and inhibitory processes and
agonist / antagonist receptor interactions in the development of medicinal cardiotonic, antiviremic
and anti-cancer agents and an understanding of their action. The special attention is given to the
structure and molecular aspects, enzyme kinetics, inactivation and reactivation mechanism, structure
– activity relationships (QSAR, graphic tecniques), drug development studies, drug release and
control mechanisms in metabolic processes. Research covers several topics: 1. The toxicology study
of biologically active compounds with the promissing pharmacological qualities (synthetic drugs,
extracts from medical plants, antitumor, antibacterial and antiviral complexes) is oriented to the in
vitro investigation between the selected compounds and aminoacids, peptides and enzymes as the
basis for better understanding of their toxic or therapeutic properties. 2. The modulation of the
activity of therapeutically and toxicologically important enzymes (ATPases, peroxidases,
cholynesterases) induced by selected compounds has been be studied, as well the indicators of
oxidative stress (lipid peroxidation, malondialdehyde). 3. The the methodological standardization of
procedures for protein isolation from human materials (erythrocytes, cell culture), enzyme
immobilization in non-conventional media and development of methods for in vitro and in vivo
evaluation of enzyme activity. Various techniques are available (stopped flow, UV VIS, UPLC, PCR,
GSMS, MALDI-TOF MS, SEM) to elucidate the structure-activity relationships, particularly the effects
of chemically well defined compounds on selected parameters of biochemical reactions.
Partners/Interests
researcher
industrial partner
Biochemistry
Toxicology
Pharmacy
Institution/University
Name of Institution/University
Vinca Institute of Nuclear Sciences
Number of Employees
800
Number of Researchers
400
Country
Serbia and Montenegro
Postal Adress
Vinca, Mike Alasa 6
11001 Belgrade
Biochemistry
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
dr. Urška Čegovnik, univ. dipl. kem
Contact Person
Name
dr. Urška Čegovnik, univ. dipl. kem
Email
[email protected]
Function
Phone
+386 4 2569 490
Fax
+386 4 25 69 117
Website
http://www.klinika-golnik.si/
Research Team Objectives
Main Fields
Biochemistry
Molecular Biology
Partners/Interests
Biochemistry
Molecular Biology
Aspirin Hypersensitivity: Sensitivity to aspirin (acetylsalicylic acid) consists of steroid-dependent
asthma and nasal polyposis (rhinosinusitis). Aspirin hypersensitivity syndrome is thought to be
caused by a decrease in the levels of the anti-inflammatory E2 prostaglandin (PG), along with an
increase in the levels of pro-inflammatory leukotrienes (LTs). Our aim is to determine the gene
expression intensity of some key proteins implicated in the pathogenesis of aspirin induced asthma
and rhinosinusitis. Intensity of gene expression is measured by Real-time PCR based on TaqMan
technology.
Institution/University
Name of Institution/University
University Clinic for Respiratory and Allergic Diseases Golnik
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Golnik 36
4204 Golnik
Biology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Dr Ljiljana Vicovac
Research Team Members
Team Member 0
MSci Zanka Bojic-Trbojevic [[email protected]]
Team Member 1
MSci Milica Bozic [[email protected]]
Team Member 2
BS Milica Jovanovic [[email protected]]
Team Member 3
MD, PhD Ljiljana Radojcic [[email protected]]
Team Member 4
MD, PhD Nebojsa Radunovic [[email protected]]
Team Member 5
MD, PhD Milos Petronijevic [[email protected]]
Research Team Projects
Project Name
Role of local factors in the implantation of the human
embryo
Project Leader
dr Ljiljana Vicovac [[email protected]]
Project Funding Agency
Ministry of Science and Technology, Serbia
Project Budget
172000 €
Project Start Date
2002-01-01
Project End Date
2005-12-31
Project Partners
Belgrade University, Medical Faculty; Medical Military Academy;
Begrade
Project Summary
Implantation of the human embryo critically depends on a strictly controled sequence of cellular
events that include adhesiveness, proliferation and invasiveness of cell populations. Deviations of the
norm are related to serious pathological conditions and may compromise pregnacy. It has been
shown that a group of extravillous cytotrophoblast cells that are in physical contact with endometrial
stroma express a specific phenotype and gain full invasiveness.Factors that regulate differentiation of
trophoblast are not suffiiently understood. The project aimed to study expression of differentiation
markers in situ in normal and transformed trophoblast and possible effects of maternal factors in
vitro, using the accepted in vitro models.
Project Website
Project Name
Cellular interactions and molecular mechanisms in
differentiation of cells at the feto-maternal interface
Project Leader
dr Ljiljana Vicovac [[email protected]]
Project Funding Agency
Ministry of Science and Environment protectio
Project Budget
250000 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Medical faculty, University of Belgrade; Medical Military Academy,
Belgrade;
Project Summary
Many aspects of the process of embryo implantation are still insufficiently understood. Physiological
invasion of uterine tissues by human placental trophoblast is crucial for embryonic development but
molecular processes involved remain largely obscure. Both shallow invasion, which is linked to the
pathogenesis of the maternal hypertensive disorder pre-eclampsia and to fetal growth retardation, as
well as the excessive proliferation and invasion cause serious and life threatening conditions.
Recently, much progress has been made regarding the trophoblast intrinsic regulatory factors and
multitude of cytokines present localy at the time of implantation. There is, however, still need for
further ellucidation of the role of some uterine and other locally present factors on invasive
trophoblast differentiation. It is proposed here to utilize different trophoblast cell models to study the
effects of the selected cytokines, chemokines, hormones or autoantibodies, on trophoblast
differentiation, apoptosis, cell migration and invasivenes. Regulatory factors and signalling pathways
Biology
will be studied in vitro at the protein and mRNA level, based on cellular markers, and functional tests.
The project will provide insights into the role of some decidual products on the differentiation of
invasive trophoblast and their relevance for normal implantation and pregnancy.
Project Website
Contact Person
Name
Dr Ljiljana Vicovac
Email
[email protected]
Function
Principal Investigator
Phone
381-11-619-252
Fax
38111618724
Website
www.inep.co.yu
Research Team Objectives
Main Fields
Biology
Obstetrics and Gynaecology
Biochemistry
Main fields of interest of the research team include: cell biology of embryo implantation, trophoblast
cell invasiveness, placental function, pregnancy related aspects of endometrial structure/function
Partners/Interests
researcher
Biology
Obstetrics and Gynaecology
Expertise in the application of molecular tools to the field of biology of embryo implantation.
Partners interested in the same field are sought for collaborative basic or applied research.
Institution/University
Name of Institution/University
Institute for the Application of Nuclear Energy INEP, B U
Number of Employees
82
Number of Researchers
29
Country
Serbia and Montenegro
Postal Adress
Banatska 31b
11080 Zemun-Belgrade
Biology
Research Team
Research Team Name
glycobiology team
Individual Researcher
No
Research Team Leader Name
senior research fellow Miroslava Jankovic
Research Team Members
Team Member 0
MSc Bojana Tapuskovic [[email protected]]
Team Member 1
MSc Maja Kosanovic [[email protected]]
Team Member 2
MSc Snezana Golubovic [[email protected]]
Team Member 3
PhD Ljiljana Hajdukovic [[email protected]]
Team Member 4
PhD Miroslava Jankovic [[email protected]]
Research Team Projects
Project Name
Glycans as molecular markers of cell function: expression,
microheterogeneity and biosignalling properties
Project Leader
PhD Miroslava Jankovic [[email protected]]
Project Funding Agency
Ministry for Science
Project Budget
240000 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
No
Project Summary
In this project we will investigate expression, microheterogeneity and biosignalling properties of
glycans as molecular markers of cell function. The special emphasis will be set on their role in
molecular patterning in prostate, ovarium, breast, colon, and placental tissue. Glycomic profiling will
comprise the common pattern of expression of N- and O-glycans, as well as the analysis of
glycoforms of molecules with known marker potential. Parallely to glyco-phenotype, the lectin
phenotype will be also analyzed, aiming at the insight in lectins as link between glyco-signals and cell
responces. Methodological approach will be based on affinity techniques using lectins and
carbohydrate-specific antibodies. The results obtained will be analyzed from aspect concerns the gain
or loss of cell function, different growth and differentiation stages, environmental influences and
immunological status, and all of this in the context of the existence of network of reactive
components in the cell, on the cell and in extracellular matrix. It is expected that the results obtained
led to better understanding of the regulatory and adaptive potential of glycosylation and to be a base
for taking part in contemporary cources of glycobiological investigations.
Project Website
Project Name
Glycobiological aspects of physiological and
pathophysiological processes
Project Leader
PhD Miroslava Jankovic [[email protected]]
Project Funding Agency
Ministry of Science
Project Budget
500000 €
Project Start Date
2002-01-01
Project End Date
2005-12-31
Project Partners
Serbia/University of Belgrade, Medical Faculty
Project Summary
The influence of endogenous and exogenous factors on synthetic and secretoty activities of human
tissues (placenta, thyroid, liver etc.) based on the expression, structural characteristics and activity of
particular types of lectins and physiologically active glycoproteins (oncofetal antigens, mucins, IGF
system) was the subject of this research.
Project Website
Contact Person
Name
senior research fellow Miroslava Jankovic
Email
[email protected]
Biology
Function
deputy director
Phone
+381 11 199 949
Fax
+381 11 618 724
Website
inep.co.yu
Research Team Objectives
Main Fields
Biology
Pathology
Clinical chemistry
Glycobiology: isolation and structural characterization of glycoproteins Lectinology: isolation,
expression and activity of endogenous lectins (galectins and C-type lectins) Tumor marker research:
development and production of solid phase binding assays (IRMA, RIA-CT, ELISA, ELBA) for
quantitative determination of glycoprotein tumor markers; expression and structural characterization
of glycoprotein tumor markers
Partners/Interests
researcher
Biology
Clinical chemistry
Pathology
Glycobiology: analysis of glycan structure (MS, glyco- and lectin-array and glycochip technology) Cell
biology Cancer biology: tumor marker characterization
1. Glyco-profiling: investigation of the expression and microheterogeneity of glycans, aiming at
differentially expressed components and establishing the correlation of their concentration to their
structural properties in terms of introduction of the multi-biomarker pattern and the formulation of
assays for their detection 2. Analysis of glycoforms of molecules with known marker potential
(oncofetal antigens, hormones, immunoglobulines etc), using lectins and carbohydrate-specific
antibodies. 3. Lectin-profiling: investigation of the expression and biosignalling properties of
carbohydrate-binding proteins, aiming at the insight in lectins as link between glyco-signals and cell
responces
Institution/University
Name of Institution/University
Institute for the Application of Nuclear Energy, INEP
Number of Employees
84
Number of Researchers
26
Country
Serbia and Montenegro
Postal Adress
Banatska 31b
11080 Belgrade
Biology
Research Team
Research Team Name
Group for immunoparasitology
Individual Researcher
No
Research Team Leader Name
MD, PhD Ljiljana Sofronic-Milosavljevic
Research Team Members
Team Member 0
MD Marija Borovic [[email protected]]
Team Member 1
M.sc Miomir Petrovic [[email protected]]
Team Member 2
B.sc, M.sc Natasa Ilic [[email protected]]
Team Member 3
B.sc., Ph.D Alisa Gruden-Movsesijan [[email protected]]
Research Team Projects
Project Name
Components of molecular communication between host and
parasite.
Project Leader
MD, PhD, Senior Research Fellow Ljiljana Sofronic-Milosavljevic
[[email protected]]
Project Funding Agency
Ministry of Science and Technology, R.Serbia
Project Budget
140716 €
Project Start Date
2002-01-01
Project End Date
2005-12-31
Project Partners
Project Summary
Trichinellosis and toxoplasmosis rank very high among all food borne parasitic diseases in Serbia in
last decades. Our research goals were: 1. to increase knowledge in basic science regarding host
immune response to infection with macro- (Trichinella) and micro-parasites (Toxoplasma), 2. To
achieve further insight in immunoregulatory and immunomodulatory mechanisms those exist during
infection, 3. To enable the scientists in the country to continue to participate the coordinated research
work on the international level regarding world wide and production of food safe meat.
Project Website
Project Name
Cellular and molecular mechanisms of immune response and
immune-regulation in parasitized host.
Project Leader
Md, PhD, Senior Research Fellow Ljiljana Sofronic-Milosavljevic
[[email protected]]
Project Funding Agency
Min. of Sci. and Environmental Protec.,Serbia
Project Budget
215000 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Project Summary
Humans co-evolved with parasites, but the complexity and medico-biological implications of this coevolution are not yet understood. Current research results are now reviling more parasitic
components that are characteristic for all metazoans, including humans, implying that there are many
direct interactions between the parasite and the physiological and immunological processes of the
host. On that way parasite could influence the host immune response not only towards itself but to
other exogenous (concomitant infectious, allergy) or even endogenous antigens (autoimmune
diseases, tumors). Recent studies emphasized the importance of the cooperative interaction between
innate and adaptive sources of factors in the generation of hypo-responsive state during some chronic
parasite infectious. The scope of this project is to examine how parasites and their products: 1.
influence the innate response of the host immune system, 2. create immunological environment that
has impact on concurrent responses to autoimmune diseases, 3. could be further characterizedand
applied in current diagnostic tools. Among expected results are: 1. better understanding on how
different Trichinella spiralis antigens influence dendritic cell maturation, B1 lymphocyte activation and
consecutive Th1/Th2 balance, 2. insight in mechanisms of modulation of autoimmune disease by
T.spiralis infection, 3. further improvements on the programs of parasitic disease surveillance and
secure food meat production.
Biology
Contact Person
Name
MD, PhD Ljiljana Sofronic-Milosavljevic
Email
[email protected]
Function
Senior research Fellow
Phone
+381 11 619 525
Fax
+381 11 618 724
Website
www.inep.co.yu
Research Team Objectives
Main Fields
Biology
Immunology and Immunohaematology
Epidemiology
Basic studies focus on host-parasite relationship, underlining immunoregulatory mechanisms and
"protective role" of infections in autoimmune and allergic disease ("worm therapy" is gaining
increasing interest in the scientific community). Applied research focuses on continuous improvement
in the diagnosis of helminthic infections enabling their surveillance and control, as well as safe food
(meat) production.
Partners/Interests
researcher
Biology
Immunology and Immunohaematology
Epidemiology
High competence in fields of immunoparasitology and molecular-biology
1.On the first place we are looking for partners in resaerch area that is described under title of
currently running project "Cellular and molecular mechanisms of immune response and immuneregulation in parasitized host" (01.01.2006-31.12.2010); 2.The role of infectious agents (parasite,
helminthes, T.spiralis) in allergy prevention; 3.Echinococcus spp., hydatidosis, immune response.
Institution/University
Name of Institution/University
Institute for the Application of Nuclear Energy "INEP"
Number of Employees
85
Number of Researchers
30
Country
Serbia and Montenegro
Postal Adress
Banatska 31b
11080 Belgrade
Biology
Research Team
Research Team Name
Institute of Biology Bucharest
Individual Researcher
No
Research Team Leader Name
Dr. Madalin Enache
Contact Person
Name
Dr. Madalin Enache
Email
[email protected]
Function
Scientific Director
Phone
+40 21 2239072
Fax
+40 21 2219071
Website
www.ibiol.ro
Research Team Objectives
Main Fields
Biology
Microbiology
Other allied sciences
Investigation of microorganisms from extreme environments (salt & acidophilic) environment;
Bioremediation of polluted soils with heavy metals and hydrocarbons
Partners/Interests
researcher
Microbiology
Biology
Other allied sciences
research in the field of microbiology
microbiology field
Institution/University
Name of Institution/University
Institute of Biology Bucharest
Number of Employees
129
Number of Researchers
102
Country
Romania
Postal Adress
Splaiul Indepnedentei Str. 169
06003 Bucharest
Biology
Research Team
Research Team Name
Laboratory for Biological Research of the Cell (Team:
Biomaterials application in Cell and Tissue Engineering)
Individual Researcher
No
Research Team Leader Name
Professor Stevo Najman
Contact Person
Name
Professor Stevo Najman
Email
[email protected]
Function
Head of Department of Biology with Human Genetics
Phone
++38118226712
Fax
++38118238770
Website
medfak.ni.ac.yu
Research Team Objectives
Main Fields
Biology
Cytology
Surgery
Biomaterials are used in more then 10% of indicated surgical interventions what opens the door to
the new technology and invention of the new biomaterials. Cell therapy, tissue engineering and gene
therapy involved in this process too. Bone skeleton became prototype for tissue engineering. The goal
of our research group is to find out optimal combination of induction signals, osteogenic cells and
matrix which can imitate natural bone tissue. Some reports showed very optimistic results when bone
substitution was used. Osteogenic cells are of mesenchimal origin. Mesenchimal cells (MC) are
multipotential stem cells and could be found in different adult tissues: fat, muscle tissue, trabecular
bone, bone marrow. Bone marrow is the most often taken as source for mesenchimal cells which can
develop in to osteoblasts, myoblasts, adipocytes and chondrocytes. Mesenchimal cells are very small
fraction (0.001%) of bone marrow cells. Both in vitro and in vivo studies showed that growth factor
can induce expansion of mesenchimal cells and their differentiation to osteoblasts. Basic conditions
for artificial matrix is biocompatibility and that it is support for cell attachment, proliferation and
migration. Ideal matrix for orthopedic use should enable formation of bone tissue and its growth,
revascularization, osteointegration with host bone and gradual replacement of artificial matrix with
newly formed bone. Also it should have adequate mechanical properties for simple regenerative
therapy. Designers and inventors of biomaterials get a heavy task to find out chemical properties,
porosity, surficial and physical properties suitable for adequate interaction between cells and
biomaterial. According to our previous results of in vivo and in vitro investigations of different
biocomposite materials like HAp/PLAA composite the one of our aims is to solve the problems of
preparation of adequate tissue matrix and its settlement with MC of bone marrow in intention to
reach satisfying osteogenic potential. Multidiscipline approach to this problem will enable to carry out
all key phases of the investigation: preparation of biomaterial, in vitro phase, in vivo tests on
experimental animals and clinical use of selected matrixes. The preparation of biomaterials will
consist of: creation of different combination of composites, modification of its surface which will
modulate interaction between cells and connective matrix. Models got in cultures of mice bone
marrow stroma cells settled to artificial matrix, will be used as base for same kind of investigations on
human bone marrow stroma cells cultures. In vitro phase usage of biomaterials is capable for
adequate morphology, biochemical, and molecular biology methods for estimation of the cell growth,
adhesion ability and differentiation ability of the cells and enables us to predict the results of
implantation. The suitable type of the cell culture medium and some supplements in medium
including specific growth factors (BNPs) are responsible for induction and differentiation of osteogenic
cells. The final estimation of osteogenic potential will be done after implantation. In vivo
investigations will be carried out on experimental animals (mice, rats, rabbits). According to the aim
of the experiment different kinds of implantation (intraperitoneal or subcutaneous) or filling up of the
bone tissue defect will be used. The animals will be sacrificed periodically and samples of grafts and
surrounding tissues analyzed in intention to make the estimation of the early regeneration period and
of the period of expected complete implant integration with tissue. Different investigation methods
will be used: post mortem examinations, pathohistology, specific staining for matrix tissue, SEM,
TEM, haematological, biochemical, ELISA and RT-PCR tests.
Partners/Interests
researcher
industrial partner
Biology
Biology
Cytology
Surgery
Institution/University
Name of Institution/University
Medical Faculty University of Nish
Number of Employees
435
Number of Researchers
340
Country
Serbia and Montenegro
Postal Adress
Boul. Dr. Z. Djindjic 81
18000 Nis
Biology
Research Team
Research Team Name
Molecular Biology
Individual Researcher
No
Research Team Leader Name
Mrs., PhD Nadja Kokalj Vokač
Contact Person
Name
Mrs., PhD Nadja Kokalj Vokaè
Email
[email protected]
Function
Professor of of Molecular Biology
Phone
+ 386 2 321 10 00
Fax
+386 2 331 23 93
Website
http://www.sb-mb.si/index.php?id=12
Research Team Objectives
Main Fields
Biology
Molecular Biology
Partners/Interests
researcher
industrial partner
Suitable
Biology
1. STUDY OF TELOMER DYNAMICS AND HUMAN TELOMERASE GENE AMPLIFICATIONS IN PREINVASIVE LESIONS AND CANCER Research work in our laboratory of medical genetics is orientated on
the problems of DNA copy numbers in human genome. Using different molecular ( MLPA : Multiplex
Ligation-dependent Probe Amplification) and molecular cytogenetic techniques (FISH : fluorescent in
situ hybridization and CGH : comparative genome hybridization) we are investigating amplifications,
duplications and deletions in the genome related to mental retardation, contiguous genetic diseases,
neuromuscular disorders and cancer. Our major interests are pre-invasive lesions and cancer. In
nearest future we will focus on the problem of telomere dynamics. A reduction of telomeric DNA is
responsible for genetic instability and could be an important factor in causation of predisposition to
developed cancer. The measurement of telomere shortening as an early somatic DNA alteration could
be useful diagnostic tool. Our project is to study the shortening of telomeres in different types of
diseases which predispose to cancer (mielodisplastic diseases, cervical dysplasia). On the other hand
activation of telomerase is also widely observed in human malignancies and is the most common
mechanism through which cancer cells stabilize their telomere size. Amplification of TERC (human
telomerase gene) was observed in various cancers. In our study we will use TERC gene as DNA FISH
probe to detect gains of TERC gene as predictive marker for invasive carcinomas of cervical cancer
and possible marker for other invasive carcinomas. New fused genes as a consequence of
chromosome rearrangements are also known as crucial in cancer development. We are interested in
one such gene TPRSS2 which fuse with ETS family members and play a role in prostate cancer. Its
fusion most likely cause overexpression of ERG or ETV1 gene. The fused protein may also exist in
other epithelial cancers. Combination of FISH and MLPA technique will be used in all our experiments.
Finding significant markers in cancer predisposition and development and application of our results in
diagnostics of cancer is a main goal of our research work.
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Biology
Research Team
Research Team Name
School of Dental Medicine University of Zagreb, Department
of Dental Anthropology
Individual Researcher
No
Research Team Leader Name
Professor Hrvoje Brkic
Research Team Members
Team Member 0
Professor Ivana Cukovic-Bagic [[email protected]]
Team Member 1
DDS, PhD Jelena Dumancic [[email protected]]
Team Member 2
DDS, MD, Assistant Marin Vodanovic [[email protected]r]
Team Member 3
DDS,MD, Assistant Andrej Katalinic []
Team Member 4
DDS, PhD Miroslav Milicevic [[email protected]]
Research Team Projects
Project Name
ANALYSIS OF TEETH IN IDENTIFICATION OF EXHUMED WAR
VICTIMS IN CROATIA
Project Leader
Professor Hrvoje Brkic [[email protected]]
Project Funding Agency
Ministry of Science, Education and Sports
Project Budget
10.000 €
Project Start Date
2002-06-01
Project End Date
2006-05-01
Project Partners
Project Summary
Achievements of civilization in the past decades resulted with a worldwide increase in human
mortality linked to mass disasters. For examples are numerous traffic accidents, especially the air
ones, followed by wars and terrorist attacks of which the worse happened recently in the United
States and resulted with loss of more than 4000 people. In order to act fast in such situations,
forensic experts of all scientific and clinical disciplines need data for fast determination of identities of
human remains. Teeth analysis is one of the basic procedures in the identitfication of unknown
person. The reason lays in the high share of anorganic substance in the composition of teeth, which
makes them the most solid and consequently most resistant part of the human body. Traces of
dentist’s interventions on teeth compared by antemortem dental characteristics such as dental charts,
x-rays, plaster casts and photographs and combined with their natural characteristics, make teeth
unique and easy to be recognized by an eye of forensic expert. Apart from the use of their
morphological recognizability, teeth are also used for different biological and biochemical researches
in the forensic procedures for the determination of identity. The use of teeth and dental tissues in the
identitfication procedures of human remains of war victims is therefore the aim of the research
proposed in this document.Teeth, extracted from human remains, will be analysed through
histological, biological and biochemical procedures for the purpose of the determinaton of victim’s
age, sex and final confirmation of the identity by the analysis of the DNA from the dental cells. The
results of this research will have their practical appliance in the procedures of the identification of
human remains exhumed from the mass graves on the territory of Croatia. On the day of writing this
project proposal it is a fact that still 1430 persons are being considered missing from the 1991 war in
Croatia. It is expected that the gains of the three years work on the proposed project will be
manifold: interdisciplinary approach of experts from different biomedical disciplines in the procedures
of the analysis of teeth and dental tissues, significant contribution in scientific research to Croatian
biomedicine, assistance to Croatian goverment in the procedures of identification of exhumed human
remains of the 1991 war victims.
Project Website
http://zprojekti.mzos.hr/zprojektiold/arh_det
Contact Person
Name
Professor Hrvoje Brkic
Email
[email protected]
Function
Vice Dean for International Cooperation
Phone
+ 385 1 4802 123
Fax
+ 385 1 4802 159
Biology
Website
http://dental.sfzg.hr/~brkic
Research Team Objectives
Main Fields
Biology
Anatomy
Dentristry
Solid dental tissues are one of the longest lasting physical evidence after death. The clinical
application of the human identity determination procedure through the analysis of teeth - after
individual and mass disasters - is based on the scientific achivements and knowledge of all
morphological, biological and biochemical characteristics of the teeth. According to the achieved
results in this research, same methods will be applied for the determination of the identities of the
living persons, as well as for the determination of the identities of the unknown human remains. They
will also be used for the putting together of the dental profile on theosealo-dental samples of the
archaeological material on the territory of the South Eastern Europe since the antique period.
Partners/Interests
researcher
Anatomy
Dentristry
Biology
Dentists, Anthropologists, Forensic experts
Human dentition in forensic and archaelogical researches in South Eastern Europe
Institution/University
Name of Institution/University
School of Dental Medicine
Number of Employees
250
Number of Researchers
150
Country
Croatia
Postal Adress
Gunduliceva 5
10 00 Zagreb
Biology
Research Team
Research Team Name
Section of Neurogenetics, Cytogenetics and Developmental
Genetics Croatian Institute for Brain Research
Individual Researcher
No
Research Team Leader Name
Professor Srecko Gajovic
Research Team Members
Team Member 0
PhD student Anja Baresic [[email protected]]
Team Member 1
MD Vesna Furic [[email protected]]
Team Member 2
PhD Marija Curlin [[email protected]]
Team Member 3
MD, PhD Dinko Mitrecic [[email protected]]
Team Member 4
Assoc. Prof. Tatjana Belovari [[email protected]]
Team Member 5
Professor Ljiljana Kostovic-Knezevic [[email protected]]
Team Member 6
medical ing. Sandra Mavric [[email protected]]
Team Member 7
medical ing. Iris Sumera []
Research Team Projects
Project Name
Gene function in differentiation and plasticity of mouse
central nervous system
Project Leader
Professor Srecko Gajovic [[email protected]]
Project Funding Agency
Ministry of science
Project Budget
50000 €
Project Start Date
2007-01-01
Project End Date
2012-01-01
Project Partners
Belgium/ULB/Lab of neuropathology Italy/ICGEB/Lab of mouse
genetics Germany/U of Hanover/Developmental Biology Unit
Project Summary
Insight in mammalian gene function in vivo will be obtained through phenotype analysis of genetically
modified mice. Molecular basis of differentiation and plasticity of the central nervous system will be
investigated on 6 mouse mutants. Pax3 (Paired box gene 3) function will be assesed in splotch mice,
which exhibit neural tube defects and spina bifida. Noto (Notochord homologue) function will be
asessed in truncate (spontaneous mutation) and NotoGFP mice (knockout), which exhibit partial lack
of notochord and disturbed differentiation of the neural tube. Stam2 (signal transducing adaptor
molecule 2), Nol1 (nucleolar protein 1) and Klf8 (Krueppel like transcription factor 8) function will be
assessed on mice obtained by gene trap method. This method involves random insertion of DNA
vector, which monitors the expression of the mutated gene with lacZ as a marker, and in addition
disturbes the production of the corresponding protein. Stam2 is involved in cell signaling through
endocytic pathway, Nol1 is involved in ribosome biogenesis in nucleolus, and Klf8 is a transcription
factor with unknown function related to mental retardation. In order to elucidate gene function in
differentiation and plasticity of the central nervous system, Pax3 and Noto activity will be revealed in
neuroectoderm differentiation through neurulation, during the closure of the posterior neuropore and
in the pathogenesis of spina bifida. Stam2 function will be investigated in the neurons in relation to
cell signaling connected with endosomal transport of cargo and membranes during differentiation and
plasticity in the brain. Molecular characterization of the mouse mutants and expression pattern
determination of the involved genes will serve as a prerequiste for the phenotype analysis. The
central nervous system phenotype of developing and adult mutant mice will give insight in gene
function in vivo. This approach will be complemented with experiments in vitro, which should disclose
the molecular and cellular basis of the observed changes. This will reveal not only the consequences
of genetic change on the system level, but as well on the cellular level. Therefore the implications of
the proposed reasearch cover genetic basis of human brain diseases, but as well basic understanding
of the gene activity and regulation during differentiation and plasticity in the central nervous system.
Project Website
Contact Person
Name
Professor Srecko Gajovic
Email
[email protected]
Biology
Function
Head of Section
Phone
+385 1 4596 829
Fax
+385 1 4596 942
Website
neurogenetika.hiim.hr
Research Team Objectives
Main Fields
Biology
Genetics
Cytology
Our main aim is to understand gene function in the mouse nervous system using mouse mutants.
Currently we concentrate on Stam2 gene, which is involved in endosome mediated cell signaling,
important for function of synapse, retrograde axonal signalling and dendrite remodelling.
Partners/Interests
researcher
Biology
Genetics
Cytology
Neuroscience, neuroimagining, phenotyping of mouse brain
Signaling pathways in the neurons are in the basis of neurodegeneration and repair. The signaling
pathways interconnection with membrane transport is poorly understood, but could be crucial for
neurons, which exhibit an intense transfer of both membranes and informations.
Institution/University
Name of Institution/University
School of Medicine University of Zagreb
Number of Employees
400
Number of Researchers
200
Country
Croatia
Postal Adress
Salata 3
10000 Zagreb
Biology
Research Team
Research Team Name
University of Osijek School of Medicine DNA Laboratory
Individual Researcher
No
Research Team Leader Name
Dr. Gordan Lauc
Research Team Projects
Project Name
Glycosylation and Human Lectins in Rheumatoid Disease
Project Leader
prof. Yuan C. Lee [[email protected]]
Project Funding Agency
NIH
Project Budget
148000 €
Project Start Date
0000-00-00
Project End Date
0000-00-00
Project Partners
USA / Johns Hopkins University / Biology Department Croatia /
University of Zagreb Faculty of Pharmacy and Biochemistry
Project Summary
Project Name
Glycogold: Exploration of the nature and potential of Glyconano-particles
Project Leader
. J.F.G. Vliegenthart [[email protected]]
Project Funding Agency
FP6
Project Budget
5000000 €
Project Start Date
0000-00-00
Project End Date
0000-00-00
Project Partners
Project Summary
Project Website
Contact Person
Name
Dr. Gordan Lauc
Email
[email protected]
Function
Professor of Biochemistry and Molecular Biology
Phone
+385 31 512 865
Fax
+385 31 505 615
Website
biochem.mefos.hr
Research Team Objectives
Main Fields
Biology
Biochemistry
Genetics
Molecular glycobiology of pathobiochemical processes
Partners/Interests
researcher
Biochemistry
Biology
Genetics
Institution/University
Name of Institution/University
University of Osijek School of Medicine
Number of Employees
100
Number of Researchers
70
Country
Croatia
Postal Adress
J. Huttlera 4
31000 Osijek
Biophysics
Research Team
Research Team Name
Biophysics
Individual Researcher
No
Research Team Leader Name
Mr., PhD Milan Brumen
Contact Person
Name
Mr., PhD Milan Brumen
Email
[email protected]
Function
Professor of Biophysics
Phone
+ 386 2 234 56 01
Fax
+ 386 2 234 56 00
Website
www.uni-mb.si
Research Team Objectives
Main Fields
Biophysics
Partners/Interests
researcher
industrial partner
Suitable
Biophysics
1. BIOPHYSICS OF CALCIUM SIGNALLING AND FORCE GENERATION IN AIRWAY SMOOTH MUSCLE
CELLS We are studying the calcium signaling pathway in airway smooth muscle cells from oscillations
of cytosolic calcium concentration to force generation in muscles. The method applied is mathematical
modeling. In particular, our studies are focused to interactions of calcium with calcium binding
proteins and relation between myosin phosphorylation and force development. Recently we published
the mathematical model of a detailed kinetic scheme describing interactions between Ca2+,
calmodulin (CaM) and myosin light chain kinase (MLCK), yielding eight different aggregates [1]. The
important result of the model is the prediction of Ca2+ dependent active form of MLCK which is in the
model taken as proportional to the concentration of Ca4CaM×MLCK complex. It presents a significant
progress in the modelling of these interactions. Based on this model we intend to extend our
theoretical studies to modelling Ca2+/CaM dependent MLCK activation in the presence of myosin as
the substrate for MLCK whereby phosphorylation and dephosphorylation of myosin is taken into
account. This step in modelling enables the prediction of MLCK activity and, thus, comparison to a
much broader set of available experimental data for MLCK activity and its relation to myosin
phosphorylation. The model will be analyzed by applying the control theory for sensitivity of the main
system variables such as myosin phosphorylation. The emphasis is given to the influence of elevated
total concentration of MLCK, the property of bronchial muscle cells obtained from asthmatic subjects
[2]. The model predictions will be directed to MLCK activation under physiological conditions. The
elevated MLCK should affect the myosin phosphorylation and, hence, the magnitude of force. It is
important also to study the time dependent response of MLCK activation with respect to calcium
oscillations especially in the range of physiologically significant low cytosolic Ca2+ concentrations. It
is desirable to build a link to a laboratory which has interest in collaborating in these topics and could
perform accompanied experiments. In the project proposed other signalling calcium dependent and
independent pathways can be studied from the point of view of their role in activities of MLC kinase
and phosphatase as well as in force generation.
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Biophysics
Research Team
Research Team Name
Center for laser microscopy; Sch of Biology; Univ. of
Belgrade
Individual Researcher
No
Research Team Leader Name
professor Pavle Andjus
Research Team Members
Team Member 0
PhD Biljana Božić [[email protected]]
Team Member 1
B.Sc. Nebojša Jasnić [[email protected]]
Team Member 2
MSc Aleksandar Bajić [[email protected]]
Team Member 3
professor Pavle Andjus [[email protected]]
Research Team Projects
Project Name
Biophysical neuroprofiling on experimental models of
damage and repair in CNS
Project Leader
prof. Pavle Andjus [[email protected]]
Project Funding Agency
Ministry of Science Rep Serbia
Project Budget
87000 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Serbia/Univ. of Belgrade/School of Biology Serbia/Univ. of
Belgrade/ Inst. for Biol. Res. "Siniša Stanković" Serbia/Univ of
Belgrade/School of Medicine Serbia/Univ. of Belgrade/School of
Physical Chemistry Belgium/Universite Libre de Bruxelles/School of
Medicine USA/Mayo Clinic, Rochester
Project Summary
Experimental models of CNS damage will be studied through the employment and development of
modern biophysical techniques -laser scanning confocal microscopy, electrophysiology ( «patchclamp»)and in vivo NMR. These techniques in synergy will be used to reach the basic conditions for
the formation of neurophysiological and biophysical profiles –«neuroprofiles» of cells and tissues in
the state of unbalanced homeostasis.The studies models will include: a) amyotrophic lateral sclerosis
(ALS)-primary cell cultures of neurones and/ or glia treated with purfied fractions of patient CSF and
sera, as well as rats with mutant CuZn superoxide dismutase;b) experimental allergic
encephalomyelitis a model of multiple sclerosis - will also serve for a comparative study (vs. ALS) of
underlying autoimmune mechanisms ; c) transgenic mice deficient in the proteoglycan tenascin - for
the study of molecular mechanisms of synaptic restoration. The expected results will put lihgt on the
role of postsynaptic receptors and glutamate transporters, on the significance of oxidative stress and
calcium homeostasis, on the intercellular connections of neurons and glia, with emphasis to the role
of glia, in the processes of injury, healing, and recovery. Neuroprofiling will be used to define
inflammatory lesions. Neuroprofiles should facilitate a closer definition of mechanisms of disease and
repair, more precise and specific diagnostics, and a more reliable introduction of therapeutic agents.
Project Website
Research Team Projects
Project Name
REINFORCING A CENTRE FOR LASER MICROSCOPY AND CELL
PROFILING FOR REGIONAL NETWORKING
Project Leader
prof. Pavle Andjus [[email protected]]
Project Funding Agency
EC FP6 & Minist. of Sci Rep. of Serbia
Project Budget
500000 €
Project Start Date
2006-06-01
Project End Date
2009-05-31
Project Partners
Serbia/Univ. of Belgrade/ Sch. of Biology Croatia/Univ. of Zagreb/
Sch. of Medicine Italy/Univ. of Turin/Dept. of Neuroscience
Italy/CNR/Inst. of Neuroscience, Pisa Norway/Univ of Oslo/Ctr for
Mol Biol and Neuroscience Slovenia/Univ. of Ljubljana/Sch. of
Medicine
Biophysics
Project Summary
The main objective of the proposal is to improve research capacities of a Western Balkan (WB)
research centre and of WBs in general, for the groundbreaking research field of cell and tissue
imaging in life sciences for health, particularly neurosciences and neurology. The WB centre, which is
the single proposer of this action, will be the Institute for Physiology and Biochemistry, School of
Biology, University of Belgrade with its Centre for laser microscopy. In addition to diverse resources
for neuroscience and physiology, the Institute is equipped with a laser scanning confocal microscope
that will present the core facility for the project research management. The principal objective will be
met through a work plan designed to 1) develop the confocal imaging core platform taking into
account specific scientific directions, i.e. physiology time-series and plasticity. development and
molecular genetics, 2) increase the capacity to participate in the research programmes of the EU and
to improve already existing links with well-established European research centres via intense
networking, 3) strengthen the technical capacity by upgrading and maintenance of equipment and by
provision of key skills, employment of young researchers and exchange visits of scientists, 4) bridge
the gap between theoretical and applied science and serve the socio-economic needs of the country
by supporting medical and pharmaceutical research through interaction with medical institutions, 5)
disseminate knowledge of cell imaging beyond the field of neuroscience and forming a wide regional
database of potential users and collaborators.
Project Website
http://clm.bio.bg.ac.yu
Contact Person
Name
professor Pavle Andjus
Email
[email protected]
Function
vice dean for science
Phone
+381-11-3032356
Fax
+381-11-2638500
Website
bio.bg.ac.yu
Research Team Objectives
Main Fields
Biophysics
Physiology
Cytology
Study of ion transport, synaptic plasticity and repair in experimental models of neurodegenerative
diseases by imaging and electrophysiology
Partners/Interests
researcher
experimental neurologist electrophysiologist expert on
Neurology
Biophysics
Physiology
experimental neurologist electrophysiologist expert on motoneuronal and related diseases and repair
expert on neuroimaging (MRI) on small animals expert on intracellular ion imaging expert on confocal
microscopy
Role of extracellular matrix (ECM) in neuroregeneration and neural plasticity. Glia-Neuron interaction
in brain repair and calcium-signalling. Nanoparticles for neuraltherapy
Institution/University
Name of Institution/University
School of Biology University of Belgrade
Number of Employees
170
Number of Researchers
74
Country
Serbia and Montenegro
Postal Adress
Studentski trg 12
11000 Belgrade
Clinical Chemistry
Research Team
Research Team Name
Department of Medical Biochemistry and Hematology,
Faculty of Pharmacy and Biochemistry, University of Zagreb
Individual Researcher
No
Research Team Leader Name
Prof. dr. sc. Tihana Zanic Grubisic
Research Team Members
Team Member 0
Prof. dr. sc. Ivana Cepelak [[email protected]]
Team Member 1
Prof. dr. sc. Karmela Barisic [[email protected]]
Team Member 2
Assist. Prof. dr. sc Lada Rumora [[email protected]]
Team Member 3
Assist. Prof. dr.sc. Jozsef Petrik [[email protected]]
Team Member 4
Assist.Prof.dr.sc. Roberta Petlevski [[email protected]]
Team Member 5
Research assistant Marija Grdic [[email protected]]
Contact Person
Name
Prof. dr. sc. Tihana Zanic Grubisic
Email
[email protected]
Function
Head of the Department for Medical Biochemistry and
Haematology,
Phone
385 1 46 12 606
Fax
385 1 46 12 716
Website
pharma.hr
Research Team Objectives
Main Fields
Clinical chemistry
Biochemistry
During the last decade, attention was oriented towards mechanisms of the cellular defence in the
development of complications in the chronic diseases, in particular toxic nephropathy and Balkan
endemic nephropathy. Currently, we are studying metabolic complications in COPD and mechanisms
of pulmonary cell destruction through activation of apoptosis. It is anticipated that apoptotic signalling
pathway involves activation of MAPK, heat shock proteins, redox-sensitive transcription factors NFkappaB, activator-protein-1 (AP-1), cytokines IL-6 and IL-8 and other inflammatory mediators. We
plan to select new markers that will be tested in COPD patients classified according to GOLD
standard. Involvement of systemic inflammation, cardiovascular effects, osteoskeletal effects,
metabolism of muscle proteins will be followed in COPD patients by using samples obtained by noninvasive methods – blood serum, cells and expired breathe condensate. The main goal of this study is
to describe optimal combination of relevant biochemical markers that could be used for diagnosis and
progression of COPD, differential diagnosis related to other respiratory diseases, therapy monitoring
and evaluating selected targets that could be used for the therapeutic purposes.
Partners/Interests
researcher
Biochemistry
Clinical chemistry
We seak partners capable of participating in the high throughput analytical analyisis needed for
metabolomic studies. We are also interested in analysis of expired breath condensate, since we only
started with that kind of studies.
We are interested to further continue on folowing metabolic changes, by means of accquiring
metabolomics data in COPD, since there is evidence that metabolic changes in the mucsles are
developing with thw progression of the disease.
Institution/University
Name of Institution/University
Faculty of Pharmacy and Biochemistry, University of Zagreb
Number of Employees
8000
Number of Researchers
2000
Country
Croatia
Postal Adress
A. Kovacica 1
10000 Zagreb
Clinical Microbiology
Research Team
Research Team Name
Department of Virology, Institute of Microbiology and
Immunology, School of Medicine, University of Belgrade
Individual Researcher
No
Research Team Leader Name
Professor Tanja Jovanovic
Research Team Members
Team Member 0
Professor Maja Cupic [[email protected]]
Team Member 1
Teaching assistant Aleksandra Knezevic [[email protected]]
Team Member 2
Teaching assistant Maja Stanojevic [[email protected]]
Team Member 3
Teaching assistant Ivana Lazarevic [[email protected]]
Team Member 4
Professor Tanja Jovanovic [[email protected]]
Research Team Projects
Project Name
Biological Consequences of Viral Genetic Variability
Project Leader
Professor Tanja Jovanovic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Project Summary
Genetic variability is one of the intrinsic properties of viruses, resulting in heterogenecity of viral
genotypes. It is mostly due to point mutations of viral nucleic acid that can affect biological properties
of viruses in a number of ways: by altering viral virulence, by modifying "fitness", by inducing escape
from host immune defences or resistance to antivirals. Primary resistance is an emerging problem in
antiviral therapy, since patients infected with drug resistant virus may experience a less efficient viral
response to therapy. Molecular epidemiology and distribution of viral genotypes, as well as frequency
and nature of antiviral resistance in our population is largely unknown, for the majority of viruses.
Our study includes molecular analysis, genotypic and phenotypic characterization of viruses inducing
persistant infections (herpes viruses - CMV, HSV, EBV etc; human papillomaviruses; HIV), hepatitis
viruses B and C and influenza virus. Thus, our study will provide the firs molecular analysis locally,
giving basic insight in molecular epidemiology of studies viruses and resistance to antivirals in our
population. Our results will contribute to development of strategies for optimal use of antiviral
treatments.
Project Website
Contact Person
Name
professor Tanja Jovanovic
Email
[email protected]
Function
Head of virology department of the Institute of microbiology
Phone
381 11 656 950
Fax
381 11 2685 584
Website
med.bg.ac.yu
Research Team Objectives
Main Fields
Clinical microbiology
Team's research interests: HIV resistance patterns in treated and untreated patients and correlation
to treatment; HBV and HCV genotyping, correlation of genotype to clinical manifestations and
mapping of resistance-associated mutations; HPV prevalence and genotyping; CMV genotyping and
mapping of resistance-associated mutations in immunocompromised hosts and in perinatal infections;
genotypic and phenotypic patterns of HSV resistance
Partners/Interests
researcher
Clinical microbiology
Epidemiology
Clinical Microbiology
Researcher in the field of clinical virology with skills and laboratory equipment for amplification,
quantitation and genetic analysis of viral nucleic acids
First insight in molecular epidemiology and resistance to antivirals in Serbia and other countries in the
region (HIV, HBV, HCV, HPV, herpesviruses, influenza virus) - genotype distribution and specific
mutants of HBV, expanding existig data on HCV heterogeneity, presence and spread of CMV
genotypes, presence and characteristics of HSV resistance to antivirals (particularly acyclovir),
heterogeneity and phylogenetic characteristics of HPV genotypes, HIV resistant patterns in treated
and untreated patients and correlation to treatment
Institution/University
Name of Institution/University
School og Medicine, University of Belgrade
Number of Employees
1000
Number of Researchers
850
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgarde
Cytology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Prof. Zoran Milosavljevic
Research Team Projects
Project Name
Forming the Living skin equvivalent and examination of its
histological, immunohistochemical and biomechanical
characteristics
Project Leader
Prof Zoran Milosavljevic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2002-01-01
Project End Date
2003-12-31
Project Partners
Project Summary
Project was aimed to reveal the cost-effective methodology for reconstructing the living skin
equvivalent in cell culture conditions. Examination of LSE histological and biomechanical
characteristics was also planned.
Project Website
Project Name
Influence of various growth factors, hormones and cytokines
of biological behaviour of the cultivated human
keratinocytes
Project Leader
Prof Zoran Milosavljevic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-08-31
Project End Date
2008-01-01
Project Partners
University Pristina, Medical School Kosovska Mitrovicy
Project Summary
Project is aimed to reveal the effects of examined factors on biological behaviour of the cultivated
human keratinocytes
Project Website
Contact Person
Name
prof zoran milosavljevic
Email
[email protected]
Function
Head of Histology and Embryology Dept
Phone
+ 381 64 124 74 61
Fax
+ 381 34 306 800
Website
www.medf.kg.ac.yu
Research Team Objectives
Main Fields
Cytology
Biology
Cytology
tissue engineering, histology, immunocytochemistry, cell culture
Partners/Interests
researcher
Biology
Pharmacy
Other allied sciences
Skin cultivation expertise, dermatology or plastic surgery specialty
Project should be aimed to reveal cost-effective and biologically safe way to obtain large quantities of
Cytology
cultivated human epidemis or living skin equvivalent in order to improve treatment options for the
patients with large skin deffects
Institution/University
Name of Institution/University
University Kragujevac Medical Faculty
Number of Employees
200
Number of Researchers
100
Country
Serbia and Montenegro
Postal Adress
Svetozara Markovica 69
34000 Kragujevac
Cytology
Research Team
Research Team Name
"Functional morphology of thymus and spleen"
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Novica Milicevic
Research Team Members
Team Member 0
Dr. Milica Labudovic-Borovic [[email protected]]
Team Member 1
Prof. Dr. Zivana Milicevic [[email protected]]
Research Team Projects
Project Name
Homeostatic control of structural integrity and size of
lymphatic organs
Project Leader
Prof. Dr. Novica Milicevic [[email protected]]
Project Funding Agency
Ministry of Science of Republic of Serbia
Project Budget
25.000 €
Project Start Date
2006-10-01
Project End Date
2010-12-31
Project Partners
Germany, University of Lübeck, Institute of Anatomy
Project Summary
Lymphotoxin-beta receptor (LTBR) signaling pathway has a crucial role in biogenesis of peripheral
lymphatic organs (PLO). Its disruption induces a profound disturbance of PLO development: Peyer
patches and lymph nodes are lacking, the splenic white pulp is disorganized. Recently, indications
appeared suggestive of role for LTBR in maintenance of structural integrity of PLO. However, in these
studies the complex methods were used and ambiguous results were obtained: the developmental
and homeostatic mechanisms could not be clearly discerned. Finally, the works dealing with
homeostatic control of PLO size are unusually rare. On the other hand, very recent studies have
shown that LTBR axis also influences the function of central lymphatic organs, i.e., the thymus. This
signaling pathway controls the activity of AIRE transcription factor, which is indispensable for proper
negative selection and suppression of autoimmunity. However, the data on structure of LTBR deficient
thymus are exceptionally few and the identity of thymic AIRE-expressing cells is not indisputably
determined. Therefore, our aim is to study: (A) the role of LTBR axis in maintenance of PLO structure
and control of PLO size in the adult organism using regeneration of splenic implants as a
straightforward and reliable model, (B) the role of LTBR axis in establishing and maintenance of
thymic structure using morpho-functional approach. Flow cytometry, immunocytochemistry and
selected in vitro methods will be used.
Project Website
Contact Person
Name
Prof. Dr. Novica Milicevic
Email
[email protected]
Function
Full Professor
Phone
+381-11-3615-772
Fax
+381-11-3612-567
Website
med.bg.ac.yu
Research Team Objectives
Main Fields
Cytology
Anatomy
Immunology and Immunohaematology
Functional morphology of the organs of the human and rodent immune system. Cellular and
structural organization of the thymus and spleen in normal and pathological conditions: after
application of toxic, cytostatic and immunomodulatory substances, after removal/absence of the
thymus or spleen, as well as in tumor necrosis factor- and lymphotoxin beta receptor-deficient
animals.
Partners/Interests
researcher
Immunology and Immunohaematology
Cytology
Cytology
-animal facility with barrier for breeding of genetically manipulated animals -cytological facilities
(tissue culture, flow cytometry, immunocytochemistry, in situ hybridisation) -microscopical facilities
(light, confocal, EM) -immunological facilities -molecular biology (PCR, western blotting,
immunoprecipitation)
My interest is particularly directed towards a special type of thymic nonlymphocyte cells – thymic
metallophilic macrophages – that we carefully studied for many years (Milićević NM, Milićević Ž.
Thymus cell-cell interactions. Int Rev Cytol 235:1-52, 2004). These cells are strategically positioned
between the cortex and medulla, forming a distinct row in the cortico-medullary zone of the rat
thymus, and show a number of characteristic features. Metallophilic macrophages seem to be
involved in thymocyte maturation: they promptly respond to the damage caused by toxic agents and
their reactivity is neatly coordinated with the subsequent thymus regeneration. Metallophilic
macrophages have overdeveloped specialized endocytic compartments for the processing and
presentation of antigens by major histocompatibility complex (MHC) class II molecules. These cells
undergo dramatic changes, if negative selection is blocked by cyclosporine A. All this suggests that
thymic metallophilic macrophages could be involved in clonal deletion of thymocytes. Therefore, I
would like to investigate this issue using various approaches: i) in vitro isolation of these cells and
testing their capacity to uptake and present antigens, ii) in vivo capability to uptake and present
antigens and iii) their reactivity in animal models of disrupted or enhanced negative selection. In a
broader context, I am interested in all aspects of studies related with the control of thymocytopoiesis
and negative selection in the thymus
Institution/University
Name of Institution/University
Inst. of Histology, Fac. of Medicine, Univ. of Beograd
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Serbia and Montenegro
Postal Adress
Visegradska 26
11000 Beograd
Dentistry
Research Team
Research Team Name
Project recognized from ministry of Science, Croatia:
"influence of prosthodontic appliance to orofacial system
and health"
Individual Researcher
No
Research Team Leader Name
Prof. PhD. Asja Celebic
Research Team Members
Team Member 0
mr.sc. Igor Stipetic []
Team Member 1
Doc.PhD. Danijela Kovacevic []
Team Member 2
mr.sc.dr. Ivan Kovacic [[email protected]]
Team Member 3
Prof.PhD. Senka Mestrovic [[email protected]]
Team Member 4
Mr.sc. Milan Papic [[email protected]]
Team Member 5
PhD. Renata Poljak Guberina [[email protected]]
Team Member 6
Mr.sc.dr. Maja Baucic Bozic [[email protected]]
Team Member 7
Prof.PhD. Jasmina Stipetic [[email protected]]
Team Member 8
Prof.PhD. Ivo Baucic [[email protected]]
Team Member 9
Mr.sc.dr. Nikola Petrièeviæ [[email protected]]
Team Member 10
doc.PhD. Dubravka Knezovic Zlataric [[email protected]]
Team Member 11
PhD. Ivan Kovacic [[email protected]]
Team Member 12
Prof.PhD. Senka Mestrovic [[email protected]]
Team Member 13
PhD. Renata Poljak-Guberina [[email protected]]
Team Member 14
Prof.PhD. Ivo Baucic [[email protected]]
Team Member 15
Higher Lect., Mr.sc. Milan Papic [[email protected]]
Team Member 16
Mr.sc. (Assistant) Maja Baucic-Bozic [[email protected]]
Team Member 17
Doc.PhD. (Ass.Prof.) Dubravka Knezovic Zlataric [[email protected]]
Team Member 18
Prof.PhD. Jasmina Stipetic [[email protected]]
Team Member 19
Mr.sc. (Assistant) Nikola Petricevic [[email protected]]
Team Member 20
Prof.PhD. Asja Celebic [[email protected]]
Contact Person
Name
Prof. PhD. Asja Celebic
Email
[email protected]
Function
Full Professor at the Department for Prosthodontics
Phone
+38514802165
Fax
+38514802159
Website
sfzg.hr
Research Team Objectives
Main Fields
Dentristry
Oral health and prosthodontic appliance
Partners/Interests
Dentristry
Institution/University
Name of Institution/University
School of dental medicine, University of Zagreb
Number of Employees
150
Number of Researchers
60
Country
Croatia
Postal Adress
Gunduliceva 5
10000 Zagreb
Dentistry
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
MSc Nedeljka Ivkovic
Research Team Members
Team Member 0
assistent Irena Mladenovic [[email protected]]
Team Member 1
Senior assistent Nedeljka Ivkovic [[email protected]]
Contact Person
Name
MSc Nedeljka Ivkovic
Email
[email protected]
Function
Senior assistent
Phone
+38765224664
Fax
+38756210171
Website
www.medfak.srbinje.net
Research Team Objectives
Main Fields
Dentristry
Prosthodontics as well as gnatology
Partners/Interests
researcher
Dentristry
Biochemistry
I would like to find partner,researcher, who has also investigeted in field of gnathology, it means,
nature and artificial occlusion, functions and dysfunctions of masticatory system
Temporomandibular disorders (TMD) are loosely defined as an assorted set of clinical conditions,
characterized by pain and dysfunction of the masticatory system. Extensive literature suggests the
disoreds is 1,5-2 times more prevalent in women than in man, and 80%of patient treted for TMD are
women. The severity od symptoms is also related to the age of patients.Pain onset tends to occure
after puberty, and peaks in the reproductive years, with the highest prevalence occuring in women
aged 20-40, and the lowest among children, adolescent, and the elderly.The gender and age
distribution of TMD suggests a possible link between its pathogenesis and female hormonal axis. The
aim of investigation would be to examine the role hormones in TMD
Institution/University
Name of Institution/University
University of East Sarajevo, Faculty of Dentistry
Number of Employees
50
Number of Researchers
20
Country
Bosnia-Herzegovina
Postal Adress
Studentska bb
73300 Foca
Dentistry
Research Team
Research Team Name
Department of Endodontics and restorative Dentistry
Individual Researcher
No
Research Team Leader Name
Prof.dr. Ivica Anic
Research Team Members
Team Member 0
prof.dr. Nada Galic []
Team Member 1
prof.dr. Greta Staudt-Skaljac []
Team Member 2
dr. stom Zoran Karlovic []
Team Member 3
prof.dr. Bozidar Pavelic [[email protected]]
Team Member 4
doc.dr. Silvana Jukic-Krmek [[email protected]]
Team Member 5
dr.stom Josipa Borcic []
Team Member 6
prof.dr. Sanja Segovic [[email protected]]
Team Member 7
prof.dr. Ivana Miletic-Karlovic [[email protected]]
Contact Person
Name
Prof.dr. Ivica Anic
Email
[email protected]
Function
Head of Department
Phone
0038514802116
Fax
0038514802159
Website
sfzg.hr
Research Team Objectives
Main Fields
Dentristry
Clinical microbiology
Epidemiology
Epidemiological testing of appearance of dilacerations, osteosclerotic and radiolucent changes will be
conducted on 2000 orthopantomograms of Zagreb's population patients. The criteria for determining
idiopatic osteosclerotic lesion would be a well defined shadow bigger than three mm of a spherical or
eliptic shape without surrounding radiolucent edge. All the others shadows with specific diagnosis
would be excluded from work. In statistical analysis parametric or nonparametric tests will be applied
depending on the distribution of obtained results. One hundred samples of mandible obtained from
Institue of Anatomy and Institute of Forensic Medicine will be used to study mandibular foramen: the
cavity size, the existence of lingua and antilingua, the distance from the lower edge od ascending
part; the distance according to the vertical from chewing bevels as well as the difference in
constitution and position of left and right side of the jaw. The following will be examined for the
mental foramen: size and position, the distance from the lower edge of the jaw, distance from the
mandibular angle, the distance between protuberantie mentalis and the ascending part of the
mandible and the difference in size and position between left and right size of the jaw. The leakage of
materials for retrograde filling will be examined by the solution for leakage assesment (bovine serum
albumin) with root end resection under the angle of 45 and 90°. While assesing the concentration of
leaked protein molecules (bovine serum albumin) the Bradford method of protein quantification is
applied. In Bradford method the protein reagent is represented by Coomassie Brilliant Blue G dye in
acidic solution. The absorption maximum of Coomassie Brilliant Blue G is moved form 465 nm to 595
nm when binding with a protein. Absorption is directly proportional to the concentration and when
determining it, spectrophotometer with the maximal transmission on 595 nm is used. The activity of
apoptotic genes after the contact with examined materials will be determined by Western Blot
Method. The induction of apoptosis will be observed by determining the number of cells with
characteristic morphological changes using the fluorescent microscope. The fragmentation of DNA (as
the proof of apoptosis induction) will be determined after the isolation of DNA with the standard
method on the agarose gel. As contol samples the same cell cultures and procedures will be used but
instead of the examined materials they will be treated with the physiological solvent. All experiments
will be repeated three times. Mutagenicity will be tested by two standard cytogenetic procedures:
analysis of the structural chromosome aberration and micronucleus test. Both procedures will be
conducted on the cultures of human peripheral blood lymphocite. The antibacterial effect of root canal
filling materials based on silicone, composite, zinc-oxide and epoxy resin will be examined by direct
contact test (DCT). During the endodontic treatment a sample from a root canal will be taken from 40
Dentistry
patients with the primary infection of root canal, clinicallly and rtg diagnosed symptomatic acute and
asymptomatic chronic apical lesions. These samples will be submitted to DNA analysis (PCR
dignostics) at Ruðer Boškoviæ Institute. The patients of both sexes, aged eighteen to sixty-five would
be included. The sample would be taken from single root and multi-rooted teeth. With the upper
molars the sample is taken from palatinal root canal while with the lower molars the sample is taken
from the distal root canal. The teeth will be isolated by coffer-dam, cleaned with 3% solution of
hydro-peroxide, then with 2.5% water solution od natrium hypoclorite and the 5% solution of natritiosulphate. After the trepanation of a pulp chamber, the samples from the root canal will be taken
with K reamer and with two sterile paper points. The points are left in the root canal for a minute and
are put into the sterile tube for transport. The obtained sample will be prepared for PCR identification.
Patients with the rtg diagnostic periapical lesion while being endodontically treated, will have root
canals filled with the preparation based on calcium hydroxide and iontophoresis will be conducted.
After the end of the treatment, after six months and after one year there will be rtg control and by
densitometrics the fomation of soft tissue will be assessed. After the instrumentation of the root
canal, sterilization of samples and artificial inoculation by standardized sort, there will be the
disinfection of canals using RinsoEndo technique, ultrasonic and classical ways of root canal irrigation.
After the experiment, the possibility of surviving and the number of inocular bacterias from root
canals will be observed by microbiological techniques. The ability of releasing calcium and hydroxil
ions from various preparations of calcium hydroxide (ready commercial preparations of various
groups) will be determined by complexometric titration and by measuring pH with pH meter Scott. As
the additional procedure of measuring ions the measuring of solution conductivity will be used. After
the endodontic filling and cementing od glass posts by various cements (zinc-phosphate, composite
and glassionomer cements) and the cyclic load the microlekage of root canal system will be examined
by fluid transport model on 65 human incisors in vitro. In odontogenic cysts the expression of Fragile
Histidine Triad (FHIT), tumor suppressor gene, will be tested on 3p14.2 locus, by
immunohystochemical method. Anti-FHIT policlone antibody will be used as well visualisation reagent
(biotinised antibody and streptavidin conjugated with peroxidas). In establishing the possible loss of
heterozigocity by PCR method, the morphogenic polymorphe markers will be used (D3S1300,
D3S4103). By pathohystologic tests the variability of odontogenic cysts constitution will be examined.
The study in laser application will determine the vibration of teeth which is a consequence of
lightening the tooth with various laser radiation energies. The vibrations during cavity restoration on
the hard dental tissues caused by laser will be determined by Piezoelectric accelerometer, also on the
soft tissues during operation and on the experimental model of hard and soft dental tissues. The
turning moment should be measured as well as the axial/vertical force during root canal
instrumentation by the following techniques: step-back, crown-down pressurreless, balanced force
technique, ProFile and ProTaper. Also, it should be determined which force and turning moment will
cause fissure or vertical fracture of the treated root. The distribution of forces occuring during
instrumentation and filling of root canals on extracted teeth will be tested on the model of upper
premolar by the finite element method. It will also be determined whether the obtained values
leading to the fracture of root wall are backed by simulation on the tooth model. The following should
also be determined: the direction of the strongest force impact on a particular root canal wall,
pressure and tensile strain during instrumentation and root canal filling. The power of force during
instrumentation will be measured by specially constructed device. The influence of the commercial
preparation for bleaching (carbamid peroxide) to the vital pulp will be tested. The treated teeth will
be premolars taken out of orthodontic reasons according to the plan of therapy. After the extraction
the pulp undergoes pathohystological examination. The testings that are conducted on biolgical
material (microorganisms, HeLa cells, fibroblasts) and the research on the material leakage begin in
the first year of the project and continue all throughout the project because of the time requirements.
The measuring of root canal filling materials leakage is repeated after one year. For epidemiological
studies are also a continuation of the previous project and will continue for the first three years of
work. The estimated time for biochemical testings on the loss of heterozygosity of FHIT tumor
suppressor gene with various odontogenic cysts is three years. Physiochemical testings will be
conducted during the whole project (three to five years). The continuous measuring od force that
appears during manual and mehanical instrumentation and the laser work and drills attached to
turbine extensions will be conducted throughout two years. The testing of root canal filling leakage
before and after the preparation of post for nonmetal intracanal restoration will last for about a year
and a half and is the continuation of work from the previous project. The measuring of vibration and
noise is the continuation of the work on the project and is meant to continue in the following two
years. The testing of disinfectant efficiency, iontophoresis and RinoEndo instruments will be
conducted throughtout three years. The measuring of the turning moment and axial force during
instrumentation and the value of forces tested by the finite element method will last for about three
and a half years. The testings in writing Master's thesis paper usually last two to three years while
those having a disertation as a goal last for three to five years. The duration estimate for the testing
of tooth pulp pathohystiological changes under the tooth bleaching materials is two and a half year.
Dentistry
Partners/Interests
researcher
Dentristry
Clinical microbiology
Epidemiology
We are seeking for reseatrches who would like to work or have previous experience in field of
endodontics, periodontology or orthhodontics (materials, biocompatibility, microbiology, imunology,
laser in dentistry).
The appearance of dilacerations, condensing ostitis and idiopatic osteosclerosis of mandible and
maxilla among Zagreb's population in relation to endodontic treatment will be assessed on 1000
orthopantomograms as well as the relation of the changes to age, sex, area and a jaw. The quality of
endodontic treatment and the appearance of pathological changes will be assessed on 1000
orthopantograms of Rijeka's population. The anthropological measurements will be conducted on 100
samples of mandible. When studying the mandibular foramen the following will be observed: foramen
size, the existence of lingua and antilingua, the distance from anterior and posterior edge of ramus;
the distance from the lower edge of ramus and the incision between articular and muscular part,
vertical distance from occlusal surfaces and of dental arch as well as the differences in the
constitution and position of a particular foramen. The following will be examined for the mental
foramen: size and position, the distance from the lower edge of the jaw; distance from the upper
edge-with complete jaws the distance from occlusive surfaces; the distance from protuberantia
mentalis and the ramus of mandible. The study on the effectiveness of iontophoresis will be carried
out on the patients who have symptomatic or asymptomatic necrosis of tooth pulp with periapical
pathosis. With the patients of the control group classical treatment procedure will be conducted while
in the experimental group iontophoresis will be applied. For microbiological testing during endodontic
treatment of the patients with the primary infection of root canals, with clinically and rtg diagnosed
symptomatic acute and asymptomatic chronical periapical lesions, the sample from the root canal will
be taken and submitted to DNA analysis (PCR). The patients of both sexes, aged 18 to 65 will be
included. To determine the effectiveness of bleaching materials there will be an investigation on
healthy teeth meant to be extracted from orthodontic reasons and the written confirmation by the
patients themselves or the patient’s guardian will be required. The bleaching will be conducted using
6% carbamide peroxide for a period of one week according to the manufacturer instructions. The
material will be applied with a brush in such a way that one tooth will be covered while the tooth on
the opposite side of the jaw will be a control tooth. The tooth is extracted for orthodontic reasons
immediately after the end of the therapy. The study results will provide data on the impact of
carbamide peroxide on the tooth pulp. Biocompatibility of root canal filling materials will be examined
by in vitro and in vivo procedures. In vitro procedures will be conducted on standardized cells. In vivo
procedures will be carried out on laboratory rats that will be anaesthetized and the material will be
implanted into subcutaneous tissue. The number, kind and position of inflammatory cells will be
investigated throughout seven and thirty days. The reaction of bone tissue will be tested using the
silicone tubes that will be implanted into the rat's bones (tibia). After 60 days the histological and
hystomorphometric analysis will be done. The endodontic materials will be tested on adheration,
fagocitosis and microbicide activity of macrophage. The sealing ability of the same materials will be
tested by bacterial leakage test and fluid transport model. The investigation will be conducted after
the periods of six months, one year and two years. The clinical studies will include the assessment of
changes on the oral mucous membrane in relation to the homogeneity of restorative and
prosthodontic materials among the senior citizens in Rijeka. The microbiological analysis of clinical
samples from oral cavity will be done. Candida species isolated from clinical material up to the level of
layer will be identified and the resistance of Candida species to dental materials will be determined.
The impact of dental materials to macrophages will be investigated in in vivo conditions on
experimental BALB/c mouses. The immunological reaction of tissue to materials and secretion of
proinflammatory factors in supernatant of primary and continuous cell cultures infected by
characteristic bacterial isolates will be investigated. The level of TNF-alpha and interleukins will be
determined by commercial EIA kit. The persons included in the epidemiological studies of periodontal
will be those above 30 years of age, of both sexes grouped according to age. The first group will
include those with cardiovascular problems and they will fall into three subgroups: with arterial
hypertension, with ischemic heart disease, those suffering from peripheral blood vessel disease.
During the study it will be possible to group them into smaller subgroups (eg. those suffering from
ischemic heart disease could be put into subgroups with angina pectoris and subgroups with
myocardial infarction). The second group will include the patients with lung diseases and conditions.
The third group will include the patients suffering from asthma, especially those under corticosteroid
therapy. Disease duration will be determined for each patient. There will be a clinical check, EKG
findings and laboratory findings. The risk factors will be determined: glucose in blood, lipidogram,
nourishment habits, smoking and the therapy during the last year. The periodontological parameters
will be measured: probing depth in millimeters for each tooth at least on four places; PBI (Papilla
Bleeding Index to determine bleeding and gingival status); recession of gingiva in millimeters from
Dentistry
vestibular and oral side of each tooth. On the basis of lists parameters the loss of attachment for each
tooth will be measured. The degree of inflammatory hyperplasion will be determined with the patients
who have been treated with antihypertension medication from nifedipine group Microbiological flora of
periodontal pocket will be determined by PCR. The five basic periodontopathogenes will be
determined by microDent© kit for each patient. Epidemiological studies on the appearance of dental
abnormalities will be carried out on 2000 orthopantograms of patients from Zagreb. The incidence of
hypodontia, hyperdontia, invagination, impaction of talon cusp, and cone shape of lateral incisors.
Measurements will be carried out on 200 orthopantomograms of Croatian patients aged between 12
and 17 by Lind method (1972). The difference between middle value of Kr/Ko proportion for contra
lateral and antagonist teeth will be studied for the entire sample and also for male and female
patients respectively. The sample in cephalometric X-ray analysis consists of 300 latero-lateral
roentgenograms of patients from Zagreb. They will be grouped according to sex, age and
abnormalities of Class II/1, Class II/2, skeletal open bite and abnormalities of progenic complex. The
same roentgenograms will be analysed manually and digitally. On the basis of study results the
normative of roentgencephalometric variables for mixed and permanent detention will be set as well
as for certain orthodontic abnormalities. The sample of photometric analysis will consist of patients
aged 25-28 without the orthodontic abnormality, proportioned profile and competent lip. They will be
photographed by digital camera. The photos will be calibrated and upon that 14 points on the face, 39
variables, out of which 27 linear and 12 angular, will be marked. Fifty patients aged 12 to 18 will be
chosen to investigate the changes in oral cavity flora, salivation changes as well as changes in the
chemical structure of saliva with patients having fixed appliances. All of them will use the ordinary
tooth paste during therapy and there will be no additional fluoridation. The saliva samples will be
taken from patients before the start of orthodontic therapy and after one, two, three, six, nine and
twelve months after the start as well as three months after the start of retention. Every sample will
be tested for the following parameters: salivary flow, pH, quantity of Str.mutans, quantity of
lactobacilli. To establish the biological bond of metal and tissue the metal ion concentration in the oral
cavity mucous membrane will be measured and biocompatibility of orthodontic appliances will be
determined as well as the possibility of DNA buccal mucous membrane impairment. The cytological
smear of buccal mucous membrane will be taken with a cytological brush after the water irrigation to
remove dead epithelial cells. Geometric and material nonlinearities will be taken into account,
differing from research performed so far. The focus will be on the physically realistic modeling of
dentin and periodontal ligament as a result of their importance in dental applications. Periodontal
ligament will be modeled by viscoelastic Ogden’s model, with necessary parameters obtained from
experimental values. Dentin, consisting of tubule within peritubular part (PTD), and enclosed by
intertubular part (ITD) will be modeled by applying continuum micromechanics approach, employing
Mori-Tanaka principle and defining Eshelby’s tensor. The ideas of micromechanical dentin formulation
by Qin and Swain will be followed. The model enables determination of dentin mechanical properties
by taking into account different volume fractions of collagen, mineral phase and fluid (liquid or gas) in
particular constituents, PTD and ITD. By knowing mechanical properties and volume fractions of
collagen and mineral, by averaging maximum and minimum values obtained by employing Voight and
Reuss models, elasticity and shear module of solid phase at PTD and ITD will be determined. The
implementation of material model into ABAQUS/Standard–Explicit finite element program will be
simplified as Eshelby’s tensor has already being numerically integrated and implemented into UMAT
subroutine, developed for computation of composite properties.
Institution/University
Name of Institution/University
School of Dental Medicine, University of Zagreb
Number of Employees
250
Number of Researchers
150
Country
Croatia
Postal Adress
Gunduliceva 5
10000 Zagreb
Dentistry
Research Team
Research Team Name
Department of Oral Medicine School of Dental Medicine
University of Zagreb and coworkers
Individual Researcher
No
Research Team Leader Name
DDS, MSc, PhD, Full professor Ana Cekic-Arambasin
Research Team Members
Team Member 0
PhD, Full professor Josip Lukac [[email protected]]
Team Member 1
MD Andjelko Vidovic [[email protected]]
Team Member 2
DMD, MSc, PhD Vanja Vucicevic Boras [[email protected]]
Team Member 3
DMD, MSc Danica Vidovic Juras [[email protected]]
Team Member 4
DMD, MSc Vlaho Brailo [[email protected]]
Team Member 5
DMD, MSc, PhD Ivan Alajbeg [[email protected]]
Team Member 6
DMD, MSc, PhD Dolores Biocina-Lukenda [[email protected]]
Contact Person
Name
DDS, MSc, PhD, Full professor Ana Cekic-Arambasin
Email
[email protected]
Function
principal investigator
Phone
0038514802114
Fax
0038514830819
Website
www.sfzg.hr
Research Team Objectives
Main Fields
Dentristry
Immunology and Immunohaematology
Pathology
Oral medicine and pathology
Partners/Interests
researcher
industrial partner.
Dentristry
Immunology and Immunohaematology
Pathology
1. Interest in ideas for this research project. 2. Experience with clinical and/or laboratory research
methods. AND/OR 3. Experience in the field of oral medicine and pathology
SALIVA AS A DIAGNOSTIC FLUID/Saliva is important element of oral and the entire organism health.
The role of many salivary constituents is still unclear and their diagnostic and prognostic potential in
oral and systemic diseases is unknown. Today's technology provides potential for salivary diagnostics
which is equal to the one of the blood. Saliva as a diagnostic fluid has many advantages in
comparison with blood which makes it a diagnostic fluid of the future. AIMS: To establish potential of
saliva in understanding, diagnosing, monitoring and therapeutic evaluation of oral diseases.
HYPOTHESIS: Saliva is susceptible to physiological and/or pathological alterations which are
influenced by aging and disease. It is assumable that diseases, particularly ones with oral
manifestations cause characteristic alterations in salivary flow and/or composition. Therefore, saliva
could be used for explaining so far unknown pathogenic mechanisms of oral diseases, for diagnostic
purposes, for follow up and therapeutic evaluation. EXPECTED RESULTS: To establish reference
ranges in healthy population of all salivary parameters which will be investigated in this project as
markers of the diseases with oral manifestations; to investigate significance of cytokines and
oncogenes in pathogenesis and malignant transformation of oral precancerous lesions; to establish
salivary markers for the follow up of periodontal disease; to confirm altered levels of salivary and/or
serum neuropeptides in BMS patients; to confirm elevated levels of salivary á-amylase as sign of
physiological stress in the etiology of RAU and OLP; to evaluate effect and safety of intraoral electrostimulator of salivary secretion; to investigate therapeutic effect of low level laser in the treatment of
xerostomia. VALIDATION OF THE RESULTS will be possible because all research protocols and
methods will be clearly defined and published and therefore reproducible. SIGNIFICANCE: Completion
of the catalogue of salivary constituents. First determination of salivary cell composition and
leukocyte subpopulations by flow cytometry. Introducing new protocols for the follow up of oral
Dentistry
premalignant lesions and early diagnosis of oral cancer. Obtaining objective markers for clinical
course and therapeutic response of periodontal disease. Establishing whether BMS is local and/or
systemic neuropathy. Defining the role of stress in the etiology of LRP and RAU. Introduction of new
therapeutic options for the treatment of xerostomia.
Institution/University
Name of Institution/University
University of Zagreb
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Croatia
Postal Adress
Gunduliceva 5
10000 Zagreb
Dentistry
Research Team
Research Team Name
Ivone Uhac's research team
Individual Researcher
No
Research Team Leader Name
DDS, PhD Ivone Uhac
Research Team Members
Team Member 0
DDS Vedrana Reljic [[email protected]]
Team Member 1
DDS Suncana Simonic-Kocijan [[email protected]]
Team Member 2
DDS, MS Miranda Muhvic-Urek [[email protected]]
Team Member 3
DDS, PhD Ivone Uhac [[email protected]]
Team Member 4
DDS, PhD Marica Simunovic-Soskic [[email protected]]
Research Team Projects
Project Name
Posttraumatic stress disorder and the function of
stomatognathic system
Project Leader
DDS PhD Ivone Uhac [[email protected]]
Project Funding Agency
MINISTRY OF SCIENCE OF REPUBLIC OF CROATIA
Project Budget
30000 €
Project Start Date
2002-08-20
Project End Date
2006-05-31
Project Partners
CROATIA/UNIVERSITY OF RIJEKA/DEPARTMENT OF
PROSTHODONTICS
Project Summary
In our previous investigations it has been reported that TMD are highly present in persons who were
exposed to war stress (Periniæ R, Uhaè I. Poredbena rasèlamba simptoma TMD-a rijeèke populacije i
prognanika na istom podruèju. Acta stomatol croat 1998;32(suppl.):166; Uhaè et al. The influence of
war stress on signs and simptoms of temporomandibular disorders. J Oral Rehabil–in print). On the
basis of those results appears a need for more detailed investigations of temporomandibular disorders
(TMD) and orofacial pain. The aim of this investigation is to establish the relation between
posttraumatic stress disoder (PTSD) and dysfunctions. The main hypothesis is that in PTSD
prerequisites for the appearance of dysfunction and orofacial pain exist. Because of the disregulation
of catecholamine and serotonin (Southwick 1999), and because the increaded muscle tone (Carlson
1997), we expect primary muscle disorders. Temporomandibular joints will be secondary included.
We expect high presence of orofacial pain because of the disregulation of serotonin (Spivak 1999)
and frequently comorbidity of PTSD and chronic pain (Sharp 2001).All the participants will be
subjected to anamnestical and clinical examination, instrumental, kinesiographic and
electromyographic registration and to the pain objectivisation, using methods whose reliability has
been previously reported (Helkimo 1974; Fricton 1986; Tsolka 1995; Farella 2000). The exameenes
with PTSD with established dysfunction of the stomatognathic system will be classified according to
the diagnostic classification of the American Academy of Orofacial Pain. They will be assembled in four
groups and submitted to different therapy for a 6 mounth period (EMG-biofeedback, stabilisation
intraoral apliances, the combination of two mtehods, and the fourth group without therapy).
Efficiency evaluation of each therapy treatment will be conducted using clinical reexaminatiom,
electromyographic and kinesiographic registration. According to the obteined results we will be able to
extend the pathophisiologic comprehensions of TMD and orofacial pain. The evaluation of each
therapy will sugges a model of simply and cheep prevention and noninvasive conservative therapy of
groups that are under incrised risk of TMD.
Project Website
www.medri.hr
Project Name
Postraumaticstressdisorder and the function of
stomatognathic system
Project Leader
DDS PhD Ivone Uhac [[email protected]]
Project Funding Agency
MINISTRY OF SCIENCE OF CROATIA
Project Budget
30000 €
Project Start Date
2002-08-20
Project End Date
2006-05-31
Dentistry
Project Partners
CROATIA/UNIVERSITY OF RIJEKA/DEPARTMENT OF
PROSTHODONTICS
Project Summary
In our previous investigations it has been reported that TMD are highly present in persons who were
exposed to war stress (Periniæ R, Uhaè I. Poredbena rasèlamba simptoma TMD-a rijeèke populacije i
prognanika na istom podruèju. Acta stomatol croat 1998;32(suppl.):166; Uhaè et al. The influence of
war stress on signs and simptoms of temporomandibular disorders. J Oral Rehabil–in print). On the
basis of those results appears a need for more detailed investigations of temporomandibular disorders
(TMD) and orofacial pain. The aim of this investigation is to establish the relation between
posttraumatic stress disoder (PTSD) and dysfunctions. The main hypothesis is that in PTSD
prerequisites for the appearance of dysfunction and orofacial pain exist. Because of the disregulation
of catecholamine and serotonin (Southwick 1999), and because the increaded muscle tone (Carlson
1997), we expect primary muscle disorders. Temporomandibular joints will be secondary included.
We expect high presence of orofacial pain because of the disregulation of serotonin (Spivak 1999)
and frequently comorbidity of PTSD and chronic pain (Sharp 2001).All the participants will be
subjected to anamnestical and clinical examination, instrumental, kinesiographic and
electromyographic registration and to the pain objectivisation, using methods whose reliability has
been previously reported (Helkimo 1974; Fricton 1986; Tsolka 1995; Farella 2000). The exameenes
with PTSD with established dysfunction of the stomatognathic system will be classified according to
the diagnostic classification of the American Academy of Orofacial Pain. They will be assembled in four
groups and submitted to different therapy for a 6 mounth period (EMG-biofeedback, stabilisation
intraoral apliances, the combination of two mtehods, and the fourth group without therapy).
Efficiency evaluation of each therapy treatment will be conducted using clinical reexaminatiom,
electromyographic and kinesiographic registration. According to the obteined results we will be able to
extend the pathophisiologic comprehensions of TMD and orofacial pain. The evaluation of each
therapy will sugges a model of simply and cheep prevention and noninvasive conservative therapy of
groups that are under incrised risk of TMD.
Project Website
www.medri.hr
Contact Person
Name
DDS, PhD Ivone Uhac
Email
[email protected]
Function
Head of Department of Prosthodontics
Phone
0038551345633
Fax
0038551345630
Website
medri.hr
Research Team Objectives
Main Fields
Dentristry
Psychiatry
Orofacial pain is one of the most common forms of the regional pain. In its acute form it is associated
to tooth or periodontal tissue. The chronic form is mainly associated with temporomandibular disorder
and atypical facial pain. The etiology and pathogenesis of the chronic forms are not fully understood
and to present time uniform therapy does not exist. The hypothesis exists that this conditions are
multicausal and are connected with trauma, stress, parafunctions and systemic disorders that play a
significant roll in their onset. The results of the numerous clinical studies that examined the influence
of various factors on the onset of orofacial pain are not conclusive. In order to assess the influence of
occlusal factors, stress and trauma on orofacial pain we will use animal model to induce and assess
the orofacial pain by one or a combination of etiological causes. The project is divided into two parts:
First part as a clinical research and second as experimental on the animal model. Our hypothesis is
that by gathering more information and knowledge of this conditions a new need for specialists and
interdisciplinary approach will arise together with the need for increased preventive measures and
regular controls. We expect to find that the experimental part will reveal or suggest exact data of the
influence of acute and chronic stress, pathological response to stress (PTSD), and bruxism on
orofacial pain and show the effectiveness of corticosteroids in treatment of orofacial pain.
Partners/Interests
researcher
industrial partner
Dentristry
Psychiatry
Pharmacology
Dentistry
same scientific interests
- examine the influence of the systemic diseases on function of stomatognatic system - examine the
influence of the acute stress on orofacial pain, i.e. on masticatory muscles and TMJ pain - examine
the influence of the chronic stress on orofacial pain, i.e. on masticatory muscles and TMJ pain examine the influence of pathological response to stress on orofacial pain, i.e. on masticatory muscles
and TMJ pain - examine the influence of microtrauma-bruxism on orofacial pain, i.e. on masticatory
muscles and TMJ pain - examine of the combined influence of multiple factors on orofacial pain, i.e.
on masticatory muscles and TMJ pain
Institution/University
Name of Institution/University
Faculty of Medicine - School of Dentistry
Number of Employees
350
Number of Researchers
100
Country
Croatia
Postal Adress
Brace Branchetta 20
51000 Rijeka
Dentistry
Research Team
Research Team Name
prosthodontic team
Individual Researcher
No
Research Team Leader Name
PhD;MSc;Prof. Melita Valentic-Peruzovic
Research Team Members
Team Member 0
prof Mario Cifrek [[email protected]]
Team Member 1
PhD Amir Catic [[email protected]]
Team Member 2
prof. Adnan Catovic [[email protected]]
Team Member 3
assistant Ivica Pelivan [[email protected]]
Team Member 4
MSc Davor Illes [[email protected]]
Team Member 5
PhD Iva Alajbeg [[email protected]]
Research Team Projects
Project Name
Analysis of function and form of stomatognathic system
Project Leader
prof. Melita Valentic-Peruzovic [[email protected]]
Project Funding Agency
Ministery of science and sport
Project Budget
15000 €
Project Start Date
2003-10-01
Project End Date
2006-06-30
Project Partners
Croatia, School of Dental Medicine, University of Zagreb,Prosthetic
Dentistry
Project Summary
electromyography (EMG), gnathology, temporomandibular dysfunction (TMD), Whiplash trauma,
optoelectronic system, echo-acoustics system for the threedimensional localisation, articulators,
bitting appliances, VAS scale, teledentistry
Project Website
www.mzt.hr
Project Name
Gnathology at net, Virtual textbook
Project Leader
prof. Melita Valentic-Peruzovic [[email protected]]
Project Funding Agency
Ministery of science and sport
Project Budget
5000 €
Project Start Date
2001-10-01
Project End Date
2002-10-01
Project Partners
Croatia, School of Dental Medicine, University of Zagreb
Project Summary
Virtual textbook of Gnathology for interactive learning and student on-line seminars
Project Website
http://gnato.sfzg.hr/
Contact Person
Name
PhD;MSc;Prof. Melita Valentic-Peruzovic
Email
[email protected]
Function
University Professor at the School of Dental Medicine
Phone
00385 1 4802 150
Fax
0038514802159
Website
sfzg.hr
Research Team Objectives
Main Fields
Dentristry
Information Technology, Statistics, Documentation
Public health services
The investigation will enable the application of new classification systems for complete and partial
edentulism in epidemiological and clinical investigations in Croatian population as well as the
Dentistry
comparison with international results. Model development for multidisciplinary data connection will
enable the referring of patients to proper diagnostic procedures. Experimental results, as well as
standardization of electromyographic registrations and acoustic analysis of occlusal sounds and TMJ
together with complete functional analysis and three dimensional kinematic model of masticatory
system, match with newest international results on masticatory system biomechanics. Cognitions in
the field of reflexology (especially emg silent period recording) are of the great interest in many
international investigation projects on craniomandibular research. Prosthodontic treatments,
especially when related to dental implants therapy, demand detailed analysis of all available biological
factors for the purpose of harmonic integration in functional unit. Facial 2D and 3D analysis using
modern digital techniques as well as its comparison with masticatory system function, match
thematically and methodologically with international scientific results.
Partners/Interests
researcher
Dentristry
Public health services
Information Technology, Statistics, Documentation
biodynamic research in dentistry Emg or axyiographic research teledentistry
Biodynamics of masticatory system and restorative procedures in dentistry- will be investigated by
using different methods such as: electromyography, gnathosonography, gnathodinamometry, 3Daccelerometry; -implementation of non-invasive objective methods for temporomandibular
dysfunction (TMD) diagnosis and also - the investigation of effects of different prosthodontic
restorations on masticatory system function and dysfunction - Changes in biodynamics and influences
of ageing process on prosthodontic restorations, neuromuscular adaptation and masticatory efficiency
will be investigated. This investigation will enable the implementation of the most contemporary
technological achievements in the field of prosthodontics by way of enabling the postulates for proper
checking of patient’s functional status. The consequences caused by unrecognised organic or
psychological patient’s condition have to be prevented on time. The aim is also to introduce specific
protocols for multidisciplinary clinical cases and provide communication and date transferring between
different clinical centres. This will enable multidisciplinary analysis and distant data transfer –
teledentistry. Our aim is also to provide diagnostic and technological support for establishing an
expert centre for Croatia and, to enable cooperation with European and international expert teams
during the project work.
Institution/University
Name of Institution/University
University of Zagreb, School of Dental Medicine
Number of Employees
120
Number of Researchers
90
Country
Croatia
Postal Adress
Gunduluceva 5
10000 Zagreb
Dentistry
Research Team
Research Team Name
department for prosthetic dentistry, unit for
temporomandibular disorder and orofacial pain
Individual Researcher
No
Research Team Leader Name
DDS, PhD Renata Grzic
Research Team Members
Team Member 0
DDS, MD Vesna Fugosic [[email protected]]
Team Member 1
DDS, MS Vlatka Mikic [[email protected]]
Team Member 0
MD, PhD Aleksandar Smokvina [[email protected]]
Team Member 1
DDS, PhD Daniela Kovacevic Pavicic [[email protected]]
Team Member 0
MD, PhD Tanja Franciskovic [[email protected]]
Team Member 1
DDS, MS Robert Antonic [[email protected]]
Team Member 0
DDS, MS Danko Bakarcic [[email protected]]
Team Member 1
MD, MS Jasna Gobic [[email protected]]
Team Member 0
DDS, PhD Renata Grzic [[email protected]]
Team Member 1
Phd, DMD Daniela Kovacevic Pavicic [[email protected]]
Team Member 0
MSc, DMD Vlatka Mikic [[email protected]]
Team Member 1
MSc, DMD Danko Bakarcic [[email protected]]
Team Member 0
DMD, MD Vesna Fugosic [[email protected]]
Team Member 1
Phd, DMD Zdravko Delic [[email protected]]
Contact Person
Name
DMD, PhD Renata Grzic
Email
[email protected]
Function
VICE DEAN, UNIVERSITY OF MEDICINE, DENTAL STUDIES, RIJEKA
Phone
00385 51 345631
Fax
00385 51 345630
Website
www.medri.hr
Research Team Objectives
Main Fields
Dentristry
Radiology
Internal Medicine
Prosthodontics, Gnathology, Internal Medicine, Nuclear Medicine, Obstetrics and Gynaecology,
Psychiatry, Radiology
Partners/Interests
researcher
Dentristry
Radiology
Internal Medicine
Have interest in this field, Have done some other research on similar or other projects connected to
this theme
Sex hormones, depending on reproductive age of the women, have an influence on the human body,
including stomatognathic system as well. They are one of the factors responsible for maintainance of
health in stomatognathic system; modify existence of chronic pain in the orofacial region, bone
mineral density of viscerocranium, state of oral mucosa and psychological status. Absence of female
sex hormones in menopause can lead to calcium homeostasis disorder, and can cause osteopenia,
even osteoporosis. Although so, in women with dentolous jaws and appropriate masticatory function
the bone loss in mandibula and maxilla is inhibited. In TMD cortisone disbalance caused by stress and
with co-existing psychological disorders, mostly depression, often cause muscle disorders. Thyroid
gland hormones regulate basal metabolism and calcium ion levels in bones and blood. The aim is to
analyze hormonal status of TSH, T4, estradiol and cortisone in women in different phases of
reproductive age and connect them to symptoms in TMD. The aim is to observe longitudinally bone
mineral density in women with natural dentition and different prosthetic appliances, comparing them
Dentistry
to sex hormone levels, hormonal status and bone density in bruxism (increased masticatory forces),
and psychological status on oral health. We expect to determine connection of hormonal and
psychological status on changes in stomatognathic system and explain it better. The role of hormonal
disbalance, emotional and social status and psychological profile compared to symptoms in TMD,
reduction in bone density, changes on oral mucosa and patient's attitude towards dental and
prosthetic health will be also better explained. RIA and IRMA Count-A-Count method will analyze
hormonal status. STAI and Beck depression and anxiety tests will evaluate psychological status. SCL
90, EPQ i PIE personality questionaries will analyze psychological symptoms. MANSA questionary will
test the quality of life. X-ray images will be digitalized. Microdensitometry method will analyze bone
mineral density. RDC/TMD questionary will evaluate signs and symptoms in TMD. The results are
expected to show influence of hormones in primary and secondary disorders, loss of bone structures
in jaws and psychological attitude, comparing them to health of the stomatognathic system. Etiology
of TMD will be better explained and therapeutic procedures will be improved.
Institution/University
Name of Institution/University
University of Medicine, Dental Studies- Rijeka
Number of Employees
350
Number of Researchers
100
Country
Croatia
Postal Adress
Kresimirova 40
51000 Rijeka
Dentistry
Research Team
Research Team Name
Endodontics
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Peter Städtler
Contact Person
Name
Prof. Dr. Peter Städtler
Email
[email protected]
Function
Phone
+43 316 3853440
Fax
Website
http://www.meduni-graz.at
Research Team Objectives
Main Fields
Dentistry
Partners/Interests
Dentistry
Institution/University
Name of Institution/University
University Clinics for Dentistry, Medical University Graz
Number of Employees
Number of Researchers
Country
Austria
Postal Adress
Auenbruggerplatz 12
8036 Graz
Dermatology
Research Team
Research Team Name
Teledermatology
Individual Researcher
No
Research Team Leader Name
H. Peter Soyer, MD
Contact Person
Name
H. Peter Soyer, MD
Email
[email protected]
Function
Phone
+43-316-385-80310
Fax
+43-316-385-4957
Website
http://www.meduni-graz.at
Research Team Objectives
Main Fields
Dermatology
Venereology
Partners/Interests
Dermatology
Venereology
Austrian tourists with skin diseases can get medical advice from University Departments of
Dermatology in Austria using a standard webbrowser and conventional digital camera or cellular
phones. In addition, a bilateral collaboration with Croatian dermatologists will facilitate the
management based on individual needs. In order to get a broad distribution for Austrian citizens
using this service a collaboration with netdoktor.at will be established.
Institution/University
Name of Institution/University
Department of Dermatology, Medical University Graz
Number of Employees
Number of Researchers
Country
Austria
Postal Adress
Auenbruggerplatz 8
8036 Graz
Epidemiology
Research Team
Research Team Name
Institute of Oncology "Prof. Dr. Al. Trestioreanu" Bucharest
Individual Researcher
No
Research Team Leader Name
Mathematician Adela Ratiu
Contact Person
Name
Mathematician Adela Ratiu
Email
[email protected]
Function
Head, Department of Medical Statistics
Phone
+40-21-6875542, +40-788-625933
Fax
+40-21-318 3262
Website
www.iob.ro
Research Team Objectives
Main Fields
Epidemiology
Information Technology, Statistics, Documentation
- cancer registries (hospital-based and population-based) - cancer statistics - cancer databases
Partners/Interests
researcher
Epidemiology
experience in hospital activities
- methods of quality control evaluation in medical reports
Institution/University
Name of Institution/University
Institute of Oncology "Prof. Dr. Al. Trestioreanu" Bucharest
Number of Employees
640
Number of Researchers
50
Country
Romania
Postal Adress
Fundeni Road, 022328 252
02232 Bucharest
Epidemiology
Research Team
Research Team Name
Belgrade MS Epidemiology Group
Individual Researcher
No
Research Team Leader Name
MD, PhD. Tatjana Pekmezovic
Research Team Members
Team Member 0
MD Jelena Kostic [[email protected]]
Team Member 1
MD Nebojsa Stojsavljevic [[email protected]]
Team Member 2
MD, MSc. Darija Kisic [[email protected]]
Team Member 3
MD, M.Sc Irena Dujmovic [[email protected]]
Team Member 4
MD, M.Sc. Sarlota Mesaros [[email protected]]
Team Member 5
MD, PhD. Mirjana Jarebinski [[email protected]]
Team Member 6
MD, PhD. Jelena Drulovic [[email protected]]
Research Team Projects
Project Name
QUALITY OF LIFE IN NEUROLOGICAL DISORDERS: GLOBAL
MEASUREMENT OF EFFECTS OF DISEASES
Project Leader
MD, PhD Tatjana Pekmezovic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Project Summary
The health-related quality of life (HRQL) measures are alternative indicators of the impact of the
disease. In this project studies will be performed to examine: 1) HRQL of selected neurological
patients in order to provide a global measure of disease impact, 2) HRQL as an outcome measure in
treatment interventions, 3) HRQL as prognostic factor and 4) validity of disease-specific HRQL
questionnaires. Additional aims of the project are assessment of the cost of neurological disorders
and analysis of the influence of disease severity and HRQL on the cost, respectively. Those studies
will be conducted in population-based cohorts and hospital-based samples. The expected outcomes of
the project comprise: 1) definition of the determinants of the HRQL in patients with selected
neurological disorders (multiple sclerosis, myasthenia gravis, neurosarcoidosis, and others) in our
population, 2) evaluated of the effects of various forms of treatments and interventions on HRQL in
our patients, 3) assessment of the potential role of HRQL as a prognostic factor for the progression of
certain neurological diseases, 4) existence of validated disease-specific HRQL questionnaires
according to specificities of Serbian language and cultural settlements, 5) estimated incidence and
prevalence of selected neurological disorders in different regions of Serbia, 6) evaluated cost of
selected neurological disorders in Serbia.
Project Website
www.mntr.sr.gov.yu
Contact Person
Name
MD, PhD. Tatjana Pekmezovic
Email
[email protected]
Function
Associate Professor of Epidemiology, School of Medicine
Phone
+381 11 3615768
Fax
+381 11 3615768
Website
med.bg.ac.yu
Research Team Objectives
Main Fields
Epidemiology
Neurology
Public health services
Our main research fields include different aspects of epidemiology of Multiple Sclerosis (MS)such as:
measurement of MS prevalence and incidence in Belgrade (population-based MS Registry established
Epidemiology
in 1996), life quality studies including assessment of treatment efficiency, studies of estimation of
global impact of the disease on the individual as well as estimation of MS cost.
Partners/Interests
researcher
Epidemiology
Neurology
Public health services
-Research experience in both life quality studies and cost evaluation studies in home countries
-To define the determinants (physical, social, emotional, and others) of the health-related quality of
life (HRQL) among patients with selected neurological disorders (multiple sclerosis, myasthenia
gravis, neurosarcoidosis, some others) -To evaluate the effects of various treatments and
interventions (immunomodulating drugs, antidepressants, surgery, physical therapy and
rehabilitation) on HRQL. -To establish the potential prognostic factors for progression of the diseases
associated with HRQL. -To estimate cost of selected neurological disorders with special emphasis on
direct and indirect costs of multiple sclerosis as: 1) one of the most common causes of chronic
disability among young adults and 2) extremely costly disease. -To incorporate HRQL instruments into
the care process.
Institution/University
Name of Institution/University
School of Medicine, University of Belgrade
Number of Employees
1172
Number of Researchers
872
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgrade
Epidemiology
Research Team
Research Team Name
Croatian Adult Health Survey 2003 - Cardiovascular Health
Individual Researcher
No
Research Team Leader Name
Professor Emeritus Silvije Vuletic
Research Team Members
Team Member 0
Assistant Ivana Kolcic [[email protected]]
Team Member 1
Assistant Ozren Polasek [[email protected]]
Team Member 2
Assistant Ognjen Brborovic [[email protected]]
Team Member 3
Assistant Aleksandar Dzakula [[email protected]]
Team Member 4
Assistant Ranko Stevanovic [[email protected]]
Team Member 5
Professor Marija Strnad [[email protected]]
Team Member 6
Assistant Sanja Music Milanovic [[email protected]]
Team Member 7
Professor Davor Ivankovic [[email protected]]
Team Member 8
Professor Josipa Kern [[email protected]]
Research Team Projects
Project Name
2003 Croatian Adulat Health Survey
Project Leader
Professor Emeritus Silvije Vuletic [[email protected]]
Project Funding Agency
WB IBRD-a 4513-0 HR
Project Budget
72000 €
Project Start Date
2002-11-01
Project End Date
2004-12-31
Project Partners
Canadian Agency for International Health
Project Summary
Project. The Health Systems Project targeted the prevention of cardiovascular disease in Croatia. One
important component of the Project is a survey of adults that provides a comprehensive assessment
of the health of Croatians, including their access to and use of health care services, health status, and
determinants of health such as smoking, physical activity, nutrition and alcohol use. The 2001
Croatian Census of Population was used to select a representative sample of households to be
included in this survey. Survey results are representative for six officially defined regions of Croatia.
The survey questionnaire was designed through consultation with many experts from epidemiologists
and public health, drawing on known survey instruments. First results were published in: Béland Y,
Bailie L, and Page J. Statistics Canada, Croatian Ministry of Health and Central Bureau of Statistics: a
joint effort in implementing the 2003 Croatian Adult Health Survey, 2004 Proceedings of the
American Statistical Association Meeting, Survey Research Methods. Toronto, Canada: American
Statistical Association, 2004.; Kern J, Strnad M, Coric T, Vuletic S. Cardiovascular risk factors in
Croatia: struggling to provide the evidence for developing policy recommendations. BMJ. 2005 Jul
23;331(7510):208-10.
Project Website
Contact Person
Name
Professor Emeritus Silvije Vuletic
Email
[email protected]
Function
Adviser Consultant
Phone
+38514590100
Fax
+38514684441
Website
www.snz.hr
Research Team Objectives
Main Fields
Epidemiology
Public health services
Social medicine
Project. The Health Systems Project targeted the prevention of cardiovascular disease in Croatia. One
Epidemiology
important component of the Project is a survey of adults that provides a comprehensive assessment
of the health of Croatians, including their access to and use of health care services, health status, and
determinants of health such as smoking, physical activity, nutrition and alcohol use. The 2001
Croatian Census of Population was used to select a representative sample of households to be
included in this survey. Survey results are representative for six officially defined regions of Croatia.
The survey questionnaire was designed through consultation with many experts from epidemiologists
and public health, drawing on known survey instruments. First results were published in BMJ (Kern J,
Strnad M, Coric T, Vuletic S. Cardiovascular risk factors in Croatia: struggling to provide the evidence
for developing policy recommendations. BMJ. 2005 Jul 23;331(7510):208-10.)
Partners/Interests
researcher
Epidemiology
Public health services
Social medicine
Researcher working on similar problems in other European countries
Regionalism of Cardiovascular Bihevioural Risk Factors The main goals of this research are to
investigate: (1) the occurrence of cardiovascular risks clusters and their regional distribution within
Croatia, (2) evolution of such clusters in individuals and their emergence on a population level, (3)
recognition of public health professionals behaviour concerning the cardiovascular health promotion,
(4) population changes of cardiovascular risk factors and mortality during the five year period, (5) to
develop a new national public health intervention model (NPHI model) based on regional peculiarities
and needs relating to the cardiovascular cluster structure. The purpose of the research is to reduce
prevalence of cardiovascular risk factors in the Croatian population, and, consequently, decrease in
the cardiovascular morbidity and mortality on a population level. The final goal of the project is to
develop regionally specific models of intervention that will be implemented by county public health
institutes, with a final goal of reducing cardiovascular burden of disease. Continuation of Croatian
Adult Health Survey every five years should be the basis for development of national public health
information system for surveillance of CVD in Croatia. This model could also be applied in other
countries, experiencing similar problems.
Institution/University
Name of Institution/University
University of Zagreb, School of Medicine, A.S. School of PH
Number of Employees
700
Number of Researchers
400
Country
Croatia
Postal Adress
Rockefellerova 4
10000 Zagreb
Epidemiology
Research Team
Research Team Name
Epidemiological study in myelodysplastic syndrome
Individual Researcher
No
Research Team Leader Name
Dr. Radu Gologan
Contact Person
Name
Dr., MD, PhD Radu Gologan
Email
[email protected]
Function
Center of Excellence of the International Foundation for MDS
Phone
+40 21 240 2020
Fax
+40 21 240 8843
Website
http://www.mds-foundation.org/mds-centers-excellence.htm
Research Team Objectives
Main Fields
Epidemiology
hematology myelodysplastic syndrome
Partners/Interests
researcher
Epidemiology
Objectives: The extending the registry of the patients with myelodysplastic syndrome existing already
at the Clinic of Hematology, Fundeni Clinical Institute at the national level. Expected results: A better
knowledge of the incidence of the myelodysplastic syndrome in Romania, the relevance of the
possible its regional variations and a better evaluations of the costs of treatments of these patients.
Impact: To report the data to the Romanian and European leading forums. An improvement of the
measures of preventing and treatment of the patients with myelodysplastic syndrome. Contribution to
the EU or regional policy: There is an increasing interest in Europe for the completing of the national
registries of the patients with different diseases. In the field of hematology, there are few European
patients registries concerning the myelodysplastic syndrome EU research: In the field of the
Myelodysplastic Syndrome there are powerful centers of care and research in Europe. An European
organization, namely already exists aiming to support the cooperation between national and regional
centers. European research potential: To compare the data obtained with those from other European
countries and to present them in the frame of Leukemia Net.
Institution/University
Name of Institution/University
Clinic of Hematology, Fundeni Clinical Institute
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Romania
Postal Adress
Sos. Fundeni 258
50096 Bucharest
Epidemiology
Research Team
Research Team Name
School of Medicine Belgrade
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Slavenka Jankovic
Research Team Members
Team Member 0
Prof. Ivana Novakovic [[email protected]]
Team Member 1
Prof. Jelena Marinkovic [[email protected]]
Team Member 2
MD, PhD Danica Bukvic [[email protected]]
Team Member 3
Ass. Dr. Natasa Maksimovic [[email protected]]
Team Member 4
Molecular biologist Nela Maksimovic [[email protected]]
Team Member 5
Ass. Dr. Momcilo Ristanovic [[email protected]]
Team Member 6
Prof. Dr. Ljubica Djukanovic [[email protected]]
Research Team Projects
Project Name
Clinical/epidemiological investigations concerning diseases
of medical and public health importance
Project Leader
Prof. Dr. Slavenka Jankovic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
We search for partners who have an expertise in clinical and fild
investigations of BEN, and who have excellence and opportunity in
pathomorphological analysis.
Project Summary
Balkan endemic nephropathy (BEN) is chronic tubulointerstitial disease of unknown etiology despite to
the numerous studies devoted to investigations of BEN etiology. It was hypothesized that both
genetic and environmental factors are involved in the development of BEN. Pathohystological studies
showed that morphological changes in BEN are non-specific and in the chronic phase they are quite
similar to changes in other chronic interstitial diseases. Several authors described changes on kidneys
arterioles in the early stage of the disease and pointed out to the similarity of BEN and cyclosporin
(CsA) induced nephropathy: multifocal microvascular hyalinosis (cyclosporin arteriolopathy) and areas
of band-like hypocellular interstitial sclerosis with tubular atrophy. Recently, numerous in vitro and in
vivo studies indicated that several fibrogenic cytokines are implicated in the pathogenesis of CsA
nephropathy. Futhermore, it was shown that CsA-induced fibrosis could be independent of renal
hemodynamics and mediated, at least partly, through angiotensin II (Ang II) induction of TGF-beta1
expression. So, Ang II, that is well known factor implicated in fibrosis, could contribute to fibrosis in
CsA nephropathy by inducing TGF-beta1. Morphological findings in BEN nephropathy indicated that
renin-angiotensin-aldosterone system (RAAS) and fibrogenic cytokines might be implicated in the
pathogenessi of the disease. Therefore, the aim of our investigation will be to examine the
involvement of angiotensin-converting enzyme (ACE), angiotensinogen (AGT), Ang II type 1 receptor
(AT1R) and TGF beta gene polymorphisms in predisposition and progression of BEN. Since the mid
1990s, many studies have been devoted to RAAS gene polymorphisms as factors that predispose to
multifactorial renal diseases. The results reported are controversial but several author groups showed
that polymorphism of the ACE gene was associated with renal disease progression. The investigation
(study) will be carried out in collaboration of Institute of Epidemiology and Institute of Biology and
Human Genetics, School of Medicine, University of Belgrade, and Institute of Endemic Nephropathy,
Lazarevac. The coworkers had great experience in field and clinical investigations of patients with BEN
(prof Ljubica Djukanovic, scientific researcher Dr. Danica Bukvic, Prof. Dr. Slavenka Jankovic), and in
genetic analysis (ass. prof. Dr. Ivana Novakovic, Ass. Dr. Momcilo Ristanovic, molecular biologist Nela
Maksimovic). The study will involve the patients with BEN, diagnosed according to criteria proposed
by Danilovic and selected in systematic investigation of inhabitants in BEN affected village in the
Kolubara region and two controls groups – one consisted of patients with kidney diseases other than
BEN and the other consisted of healthy persons. Detailed clinical, ultrasound and biochemical
investigations of patients will enable to analyze relevance of genetic risk factors in the context of
clinical and biochemical variables. DNA study will start by collection of DNA samples from persons
belonging both BEN and control groups. Analysis of selected polymorphisms of ACE, AGT, AT1R and
Epidemiology
TGF beta genes will be based on simple PCR method for deletion/insertion polymorphism, and
PCR/RFLPS method or PCR/ARMS method for single nucleotide polymorphisms (SNPs). Detected allele
frequencies and genotypes will be compared within groups and possible association of gene
polymorphisms with other clinical, ultrasound and biochemical parameters in BEN will be studied. The
studies directed to examination of the role of the RAAS in the expression of TGF-beta1 and other
fibrogenic cytokine as well as matrix proteins in renal tissue of patients with the early phase of BEN
would be reasonable.
Project Website
Contact Person
Name
Prof. Dr. Slavenka Jankovic
Email
[email protected]
Function
Professor
Phone
00381112164794
Fax
00381113615768
Website
http://www.med.bg.ac.yu
Research Team Objectives
Main Fields
Epidemiology
Internal Medicine
Genetics
Our main fields include clinical, epidemiological and genetic investigation of Balkan endemic
nephropathy
Partners/Interests
researcher
Epidemiology
Internal Medicine
Genetics
We search for experts in clinical and field investigation of BEN and partners that have excellence and
opportunity in pathomorphological analysis.
Detailed clinical, ultrasound and biochemical investigations of patients with Balkan endemic
nephropathy will enable to analyze relevance of genetic risk factors in the context of clinical and
biochemical variables. The studies directed to examination of the role of the RAAS in the expression
of TGF-beta1 and other fibrogenic cytokine as well as matrix proteins in renal tissue of patients with
the early phase of BEN would be reasonable.
Institution/University
Name of Institution/University
Institute of Epidemiology, University of Belgrade
Number of Employees
14
Number of Researchers
11
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgrade
Epidemiology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Dr sci. Jadranka DIZDAREVIĆ
Research Team Members
Team Member 0
Dr sci. Jadranka DIZDAREVIĆ
Team Member 1
Fadila Serdarević MD,MPH
Team Member 2
Semir Vranić MD
Team Member 3
Jasmin Ćeranić, MD,MBA
Team Member 4
Goran Stojkanovic, MD
Team Member 5
Mohamed Ardat MD
Team Member 6
Olga Gurjeva, MD, PhD
Contact Person
Name
Dr sci. Jadranka DIZDAREVIĆ
Email
[email protected]
Function
professor, Department of Gynaecology
Phone
+33 663 742 / 743
Fax
+33 203 670
Website
www.mf.unsa.ba
Research Team Objectives
Main Fields
Epidemiology
Low Genital Tract Infections, Screening, Preterm Delivery, South Eastern Europe
Partners/Interests
Is there a need for routine antenatal screening on Low Genital Tract Infections in South
Eastern Europe?
Prevalence of low genital tract infections (LGTI) in South Eastern Europe is unknown. The combined
impact of poverty, a high rate of unemployment, deteriorating economic conditions, decline in
provision of basic services, conflicts and migration has resulted in the presence of increased risk in
this region.
In resource - poor settings, managing of LGTI is challenging in terms of diagnostic accuracy and
appropriate treatment. To date no systematic strategies have been developed for this vulnerable
population in the area of LGTI awareness and prevention.
The proposed investigation will undertake a multi-center multi-stage cluster Primary Health Care
(PHC) based cross-sectional survey, including screening for Low Genital Tract Infections biomarkers,
in order to asses the relationship between most important risk factors, presence of symptoms,
awareness of symptoms and Trichomonas Vaginalis, BV, Chlamidia Trachomatis, Gonorrhea, Candida
Albicans among pregnant women in South Eastern Europe, coming for routine gynecological
examination between 17-24 gestational week.
The aim of this study is to determine the prevalence of lower genital tract infection by analysis of
cervico-vaginal secret and the prevalence of symptoms in pregnant women. We want to explore
whether the routine screening for the most common LGTI is warranted regard to the costs and
potential benefits of screening the asymptomatic LGTI infections that might cause preterm delivery as
a main cause of both fetal and maternal morbidity and mortality.
Institution/University
Name of Institution/University
University of Sarajevo, Faculty of Medicine Sarajevo
Number of Employees
Number of Researchers
Country
Bosnia and Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Genetics
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
DVM, PhD, senior scientist Nela Pivac
Research Team Members
Team Member 0
BSc Martina Deželjin [[email protected]]
Team Member 1
senior scientist Dorotea Muck-Šeler [[email protected]]
Team Member 2
BSc Maja Mustapic [[email protected]]
Contact Person
Name
DVM, PhD, senior scientist Nela Pivac
Email
[email protected]
Function
senior scientist at Rudjer Boskovic Institute
Phone
385 1 4571 207
Fax
385 1 456 1010
Website
www.irb.hr
Research Team Objectives
Main Fields
Genetics
Pharmacology
Psychiatry
biological and genetic basis of posttraumatic stress disorder, acute stress reaction, alcoholism, ADHD
and suicidal behaviour
Partners/Interests
researcher
Psychiatry
Genetics
Pharmacology
collaboration in the research
the research on the psychobiological factors in psychiatric disorders
Institution/University
Name of Institution/University
Rudjer Boskovic Institute
Number of Employees
300
Number of Researchers
400
Country
Croatia
Postal Adress
Bijenicka 54
10000 Zagreb
Genetics
Research Team
Research Team Name
Endocrine oncology and hereditary tumors
Individual Researcher
No
Research Team Leader Name
MD, PhD Svetozar Damjanovic
Research Team Members
Team Member 0
mol. biologist, Bsci Tatjana Perisic []
Team Member 1
mol. biologist, PhD Jadranka Dundjerski []
Team Member 2
mol. biologist, PhD Gordana Matic []
Team Member 3
mol. biologist, Bsci Katarina Mirkovic []
Team Member 4
mol. biologist, Bsci Jelena Nikolic []
Team Member 5
mol. biologist, Bsci Jovana Vignjevic []
Team Member 6
mol. biologist, Bsci Jadranka Antic []
Team Member 7
MD, Msci Bpjana Beleslin []
Team Member 8
MD, Bsci Ivana Bozic []
Team Member 9
MD, Bsci Tanja Isailovic []
Team Member 10
MD, Bsci Bojana Popovic []
Team Member 11
MD, Msci Sanja Ognjanovic []
Team Member 12
MD, PhD Milan Petakov []
Team Member 13
MD, PhD Djuro Macut []
Team Member 14
mol. biologist, Bsci Ivana Elakovic []
Team Member 15
MD, PhD Ivan Paunovic []
Research Team Projects
Project Name
Psychobiology of posttraumatic stress disorder
Project Leader
MD, PhD Eric Vermetten [[email protected]]
Project Funding Agency
EC, FP6
Project Budget
1300000 €
Project Start Date
2004-10-01
Project End Date
2007-10-01
Project Partners
Serbia, Institute for Nuclear Sciences, Belgrade University Serbia,
International Aid Network, Belgrade Serbia, Institute for Biological
Research, Belgrade University, Biochemistry UK, Queen Mary and
Westfield College-University of London, Psychiatry Italy, Universita
Degli Studi di Bari, Psychiatry Croatia, Meical School of Rijeka,
University of Rijeka
Project Summary
It is likely that increased HPA axis sensitivity can be connected with specific glucocorticoid receptor
gene polymorphism. Long term activation of HPA axis in patients with PTSD could be associated with
a) impaired or normal cortisol suppression with lower doses of Dexamethasone, b) central obesity, c)
hypertension and d) inhibitory effects on secretion of sex steroids and growth hormone. Differential
response to low dose of Dexamethasone with hypothetically different pattern of POMC cleavage in
health and illness could be of help in making difference between subtypes of PTSD. Visceral obesity,
insulin resistance and hypertension are major components of metabolic syndrome. Number of
patients with PTSD has the same characteristics and all patients with hypercortisolism (Cushing’s
syndrome). Depression, so common in Cushing’s syndrome can be found as a co-morbid state in
patients with PTSD. PTSD and metabolic syndrome, therefore, may have in common functional
properties of glucocorticoid receptor (GR) due to specific gene BclI polymorphism within the intron
upstream of GR exon 2, which is associated with visceral obesity, hypertension, insulin resistance and
elevated cortisol levels. Thus, we expect to find specific changes in anthropometric measurements of
patients with PTSD and higher incidence of hypertension and obesity.
Project Website
Genetics
Project Name
Psychobiology of posttraumatic stress disorder
Project Leader
MD, PhD Eric Vermetten [[email protected]]
Project Funding Agency
EC, FP6
Project Budget
1300000 €
Project Start Date
2004-10-01
Project End Date
2007-10-01
Project Partners
Serbia, Institute for Nuclear Sciences, Belgrade University Serbia,
International Aid Network, Belgrade Serbia, Institute for Biological
Research, Belgrade University, Biochemistry UK, Queen Mary and
Westfield College-University of London, Psychiatry Italy, Universita
Degli Studi di Bari, Psychiatry Croatia, Meical School of Rijeka,
University of Rijeka
Project Summary
It is likely that increased HPA axis sensitivity can be connected with specific glucocorticoid receptor
gene polymorphism. Long term activation of HPA axis in patients with PTSD could be associated with
a) impaired or normal cortisol suppression with lower doses of Dexamethasone, b) central obesity, c)
hypertension and d) inhibitory effects on secretion of sex steroids and growth hormone. Differential
response to low dose of Dexamethasone with hypothetically different pattern of POMC cleavage in
health and illness could be of help in making difference between subtypes of PTSD. Visceral obesity,
insulin resistance and hypertension are major components of metabolic syndrome. Number of
patients with PTSD has the same characteristics and all patients with hypercortisolism (Cushing’s
syndrome). Depression, so common in Cushing’s syndrome can be found as a co-morbid state in
patients with PTSD. PTSD and metabolic syndrome, therefore, may have in common functional
properties of glucocorticoid receptor (GR) due to specific gene BclI polymorphism within the intron
upstream of GR exon 2, which is associated with visceral obesity, hypertension, insulin resistance and
elevated cortisol levels. Thus, we expect to find specific changes in anthropometric measurements of
patients with PTSD and higher incidence of hypertension and obesity.
Project Name
Psychobiology of posttraumatic stress disorder
Project Leader
MD, PhD Eric Vermetten [[email protected]]
Project Funding Agency
EC, FP6
Project Budget
1300000 €
Project Start Date
2004-10-01
Project End Date
2007-10-01
Project Partners
Serbia, Institute for Nuclear Sciences, Belgrade University Serbia,
International Aid Network, Belgrade Serbia, Institute for Biological
Research, Belgrade University, Biochemistry UK, Queen Mary and
Westfield College-University of London, Psychiatry Italy, Universita
Degli Studi di Bari, Psychiatry Croatia, Meical School of Rijeka,
University of Rijeka
Project Summary
It is likely that increased HPA axis sensitivity can be connected with specific glucocorticoid receptor
gene polymorphism. Long term activation of HPA axis in patients with PTSD could be associated with
a) impaired or normal cortisol suppression with lower doses of Dexamethasone, b) central obesity, c)
hypertension and d) inhibitory effects on secretion of sex steroids and growth hormone. Differential
response to low dose of Dexamethasone with hypothetically different pattern of POMC cleavage in
health and illness could be of help in making difference between subtypes of PTSD. Visceral obesity,
insulin resistance and hypertension are major components of metabolic syndrome. Number of
patients with PTSD has the same characteristics and all patients with hypercortisolism (Cushing’s
syndrome). Depression, so common in Cushing’s syndrome can be found as a co-morbid state in
patients with PTSD. PTSD and metabolic syndrome, therefore, may have in common functional
properties of glucocorticoid receptor (GR) due to specific gene BclI polymorphism within the intron
upstream of GR exon 2, which is associated with visceral obesity, hypertension, insulin resistance and
elevated cortisol levels. Thus, we expect to find specific changes in anthropometric measurements of
patients with PTSD and higher incidence of hypertension and obesity.
Contact Person
Name
MD, PhD Svetozar Damjanovic
Email
[email protected]
Genetics
Function
Professor of internal medicine/Director of Institute
Phone
+381 11 2684177
Fax
+381 11 2685357
Website
med.bg.ac.yu
Research Team Objectives
Main Fields
Genetics
Internal Medicine
We are generally involved in endocrine oncology, mostly in neuroendocrine tumors (NETs) including
hereditary syndromes, multiple endocrine neoplasia type 1 and 2 (MEN 1 and MEN 2). We perform
genetic screening (MENIN gene and RET proto-oncogene) in these patients and their families in a
regular way. Regular screening, in patients with apparently sporadic pheochromocytoma, for known
and new mutations in genes encoding succinate dehydrogenase subunits B, C and D (SDHB, SDHC
and SDHD) is also performed. The role of mutations in VHL and transcriptional activity of VHL, HIF-a,
and Erythropoetin genes in tumorigenesis of clear renal cell carcinoma is another project that we are
interesting in. Adrenal tumors (adenomas) that are incidentally discovered seem to be associated with
insulin resistance. We study therefore the role of glucocorticoid receptor gene polymorphisms (BclI,
N363S and ER22/23EK) in the development of insulin resistance in patients with adrenal adenomas.
We are open for the cooperation in all fields of our work and research.
Partners/Interests
researcher
Genetics
Physiology
Internal Medicine
1. Partner should be able to provide patients. 2. To have facilities to performe genetic analyses or to
be able to send tissue samples to our labs. 3. To enable metabolic and endocrinological assessment of
patients
The Impact of Glucocorticoid Receptor (GR) Gene Polymorphisms N363S, Bcl-I and ER22/23EK on
Insulin Resistance in Patients with Clinically Non-Functioning Adrenal Tumors Clinically inapparent
adrenal masses detected trough imaging for non-adrenal desease, are often refered to as adrenal
incidentaloma, were first described 20 years ago. Despite the rarity of primary adrenal carcinoma,
incidentaly discovered adrenal masses are one of the most prevalent of all tumors in humans. The
prevalence of incidentalomas approches 3% in middle age, and increases to as much as 10% in the
elederly. Adenomas comprise the vest majority of incidental asymptomatic adrenal masses. According
to literature, up to 47% (5-47%) secrete cortisol and it seems that these patients are at increased
risk for the development of metabolic syndrome. The impact of these tumors on health outcome is
undetermined. This should be a prospective study. In patients with established diagnosis of so called
non-functional adrenal tumor, confirmed by proper clinical (endocrine testing) and radiological
evaluation (MRI or CT), the impact of GR genotype on sensitivity to glucococrticoids (GC) and insulin,
and on body composition should be assessed. GCs play important role in the process of differentiation
and proliferation of visceral fat tissue. On the ohter hand, the amount of visceral fat tissue is the key
determinant of insulin resistance. Thus, we expect to find close relationship between GR gene
polimorphisms and specific phenotypes in these patients (body composition and insulin resistance). It
is likely, that we shall be able to develop specific clinical tools for the detection of patients at higher
risk for development of early atherosclerosis. In all patients RFLPs, SSCP and automated sequencing
techniques will be used in genotyping of GR gene in constitutive DNA (blood leukocytes). In those
patients who will undergo surgery, genotyping of GR gene will be performed by using DNA obtained
from normal adrenal-cortex tissue in adjacent to adrenal adenoma and from tumor tissue. In
addition, transcriptional activity (mRNAs) of heat shock protein (Hsp) 70 and 90 genes and GR gene
will be determined by RT PCR in tumor tissue as well as in normal adrenal cortex in adjacent to
tumor. The expression of Hsp70, Hsp90 and GR in normal and tumor tissue will be determined by
western blot. Body composition will be assessed by DEXA and hormonal/metabolic parameters by
ELISA, RIA, IRMA and HPLC. New knowledge could be generated and its application might have some
impact on health policy of every country in EU through novel pharmaceutical approach in the
treatment of, not only adrenal incidentalomas but also of obesity and insulin resistance. Results of
this project might add novel information to the pathogenesis of adrenal tumors and early
atherosclerosis. These can be converted into social and economic benefits through
prevention/retardation of both diseases. To convey this project, collaboration not only between
different specialties in medicine is needed, but it also encompasses collaboration between centers of
excellence all over the EU.
Genetics
Institution/University
Name of Institution/University
Institute of Endocrinology, Medical School of Belgrade
Number of Employees
300
Number of Researchers
45
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 13
11000 Beograd
Genetics
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Associated Professor Visnja Lezaic
Research Team Members
Team Member 0
Associated Professor Visnja Lezaic [[email protected]]
Team Member 1
Retired professor Ljubica Djukanovic [[email protected]]
Team Member 2
M.DD. Ph. D. Milan Stosovic [[email protected]]
Team Member 3
teaching assistant Radomir Naumovic [[email protected]]
Team Member 4
teaching assistant Milan Radovic [[email protected]]
Team Member 5
Associated Professor Sanja Simic-Ogrizovic [[email protected]]
Team Member 6
Associated Professor Dijana Jovanovic [[email protected]]
Research Team Projects
Project Name
KIDNEY DISEASES IN THE ELDERLY: DEVELOPMENT OF
STRATEGY FOR EARLY DETECTION, PREVENTION AND
TREATMENT
Project Leader
Associated Professor Višnja Ležaić [[email protected]]
Project Funding Agency
Ministry of Science and technology of Serbia
Project Budget
20000 €
Project Start Date
2006-01-01
Project End Date
2010-01-01
Project Partners
University Clinical Centre, Institutes of Urology and nephrology,
Department of Nephrology and Biochemistry, University of
Belgrade, School of Medicine, Institutes of Biochemistry and Biology
and Human Genetics, Serbia
Project Summary
A rapid growth in the number of elderly persons requiring dialysis therapy made the investigations of
kidney diseases in the elderly very actual nephrology topic. The aim of the project is to find out the
characteristics of kidney diseases in persons over 65 and to define the strategies for their early
detection, prevention and treatment. The objective of the study comprises epidemiological, functional,
clinical, laboratory investigations of kidney disease in elderly patients and the comparison with
patients younger than 65. The investigations will reveal: 1. Prevalence of kidney disease patients over
65 treated in nephrology units in Serbia and in the primary health care units, 2. The most accurate
method for estimation of global kidney function and the influence of aging, kidney diseases and comorbidity on kidney size and function, 3. Specificity of acute, chronic renal failure, ESRD and
glomerular diseases in the elderly and the most effective methods for their treatment, 4. Frequency,
clinical significance and possibilities for the prevention and therapy of co-morbidity in the elderly
ESRD patients, 5. Mechanism contributing to atherosclerosis, the most prevalent co-morbidity in CRF
6. Association between depression, chronic inflammation, quality of life, morbidity, mortality of ESRD
patients over and below 65. The investigations will allow the definition of the guidelines for the early
detection, prevention and treatment of kidney diseases in the elderly in our country.
Project Website
Contact Person
Name
MD Visnja Lezaic
Email
[email protected]
Function
Chief of the department
Phone
+381 11 3617127
Fax
+381 11 3617127
Website
http://www.med.bg.ac.yu
Genetics
Research Team Objectives
Main Fields
Genetics
Clinical chemistry
Internal Medicine
Premature atherosclerosis and cardiovascular calcifications in patients with chronic renal failure
and/or treated with dialysis was driven by more than one biochemical pathway. Besides patient age,
inflammation and calcium-phosphate disturbances, genes encoding various proteins (involved in the
inflammatory component of atherosclerosis) have major influence The scientific excellence of the
group is proven by the bibliographic entries. Major equipment for biochemical and genetic laboratory
is available.
Partners/Interests
researcher
Genetics
Clinical chemistry
Internal Medicine
We search for researchers/ partners interested in investigations of cardiovascular disturbances in
chronic renal failure patients in predialysis phase or treated with dialysis.
Premature atherosclerosis and cardiovascular calcifications in patients with chronic renal failure
and/or treated with dialysis was driven by more than one biochemical pathway. Besides patient age,
inflammation and calcium-phosphate disturbances, genes encoding various proteins (involved in the
inflammatory component of atherosclerosis) have major influence The scientific excellence of the
group is proven by the bibliographic entries. Major equipment for biochemical and genetic laboratory
is available.
Institution/University
Name of Institution/University
Clinical Centre of Serbia, Institute of Urology and Nephrology
Number of Employees
400
Number of Researchers
40
Country
Serbia and Montenegro
Postal Adress
Pasterova 2
11000 Beograd
Genetics
Research Team
Research Team Name
1. Rudjer Boskovic Institute, Division of Molecular medicine
2. Private clinic for maxillofacial surgery "Bagatin"
Individual Researcher
No
Research Team Leader Name
Assistant Professor, M.D, PhD. ENT speci Jadranka Handzic
Research Team Members
Team Member 0
MSc. Sandra Kraljevic [[email protected]]
Team Member 1
MSc. Mirela Sedic [[email protected]]
Team Member 2
Dipl.ing. Sinisa Bratulic [[email protected]]
Team Member 3
Professor, PhD. M.D. Kresimir Pavelic [[email protected]]
Team Member 4
Mario Bagatin []
Team Member 5
M.D, PhD. Jadranka Handzic []
Team Member 6
M.D. veterinarian Srdan Vucinic []
Contact Person
Name
Assistant Professor, M.D, PhD. ENT specialist Jadranka Handzic
Email
[email protected]
Function
Medical School, University of Zagreb, Faculty
Phone
00 385 1 4552333
Fax
00 385 1 4552333
Website
www.mef.hr
Research Team Objectives
Main Fields
Genetics
Otorhinolaryngology
Functional genomics analyses of craniofacial structures in clinically well defined subgroups of patients
with non-syndromic cleft lip and palate
Partners/Interests
researcher
Genetics
Otorhinolaryngology
Genetics, microarray analyses, non-syndromic cleft lip and palate
Non-syndromic cleft lip and palate are accompanied with developmental changes of the cranial base,
retrognathic maxilla, increased pharyngeal width, smaller middle ear space, changes in petrous
portion of the temporal bone, short and high positioned and clefted hard palate, hypoplastic and
malpositioned cleft muscles and retarded mastoid pneumatisation with low or no tendency for growth
which is in correlation with the severity of cleft lip and palate. The major consequence of these
anatomical abnormalities is an increased risk of conductive hearing loss, caused by otitis media with
effusion. Its long term presence leads to impairment of central auditory hearing as well as cognitive
and behavioral disturbances joined with problems in speaking and social adaptability. In addition,
poor detection of sounds in noisy environments and poor auditory temporal resolution are very
frequent findings. Surgery of cleft lip and palate is mostly focused on reconstruction of cleft structures
and aesthetic point of view with minor or no interest in functional improvement. In most patients,
there is a need for secondary reconstructions and prolonged rehabilitation, which dramatically
increases the cost of the whole procedure. The comprehensive effort in recognizing the etiology of
most, if not all, disturbances through the research of gene polymorphisms and/ or genes activity
should be the ultimative goal in this area of research. So far, there are no many information on
genetic background underlying this disorder. The literature data mostly point out the polygenic
disease which occurs as the consequence of interaction of harmful environmental factors with specific
genetic background. One can conclude that one of the major missleading in searching for the
gene/genes candidates might be lack of understanding of different clinical presentations/phenotypes
in these patients. Hence, detailed study of phenotypes is the first and only valuable base for
understanding the genotype. The analysis of all craniofacial abnormal anatomy features accompanied
to cleft lip and palate and combined with careful re-grouping of patients is an imperative. The results
of functional genetics analyses of the higest quality can answer specific question only if performed on
clinically well selected patients. This is particularly important for DNA polymorphism analyses. Not
only prevention, but also the treatment of cleft lip and palate should be based on multidisciplinary
Genetics
basic and clinical research. This approach should not help only on early recognizing of this disorder,
but will also lead to efficient therapy in routine clinical work which is a guarantee for better health and
social integration. These goals can be accomplished only through comprenhensive genomic and
proteomic research.
Institution/University
Name of Institution/University
Medical School, University of Zagreb
Number of Employees
500
Number of Researchers
300
Country
Croatia
Postal Adress
Salata 4
10000 Zagreb
Genetics
Research Team
Research Team Name
Asthma Genomics
Individual Researcher
No
Research Team Leader Name
DSc Csaba Szalai
Research Team Members
Team Member 0
MSc Ildiko Ungvari [[email protected]]
Team Member 1
MSc Gergely Tolgyesi [[email protected]]
Team Member 2
PhD, MD Adrienne Nagy [[email protected]]
Team Member 3
MSc Marton Keszei [[email protected]]
Research Team Projects
Project Name
Genetic background of Asthma
Project Leader
DSc Csaba Szalai [[email protected]]
Project Funding Agency
OTKA,. ETT
Project Budget
28000 €
Project Start Date
2004-01-01
Project End Date
2008-12-31
Project Partners
Hungary/Semmelweis Univ/DGCI Hungary/Heim Pal Hospital/Mol
Biol Lab Hungary/Hungarian Academy of Sciences/Res group of
Immunogenomics
Project Summary
Asthma genetics and genomics Aim: 1. With the help of large number of asthmatic and healthy
patients and SNP screening methods we would like to identify genes and genetic variations playing
roles in the pathomechanism of and susceptibility to asthma. A Core Facility for high throughput SNP
screening (GenomeLab SNPstream with the capacity of several million SNPs in a day) is available for
us, which provides the possibility to identify unknown genes or genome areas in asthma. These genes
and their protein products are potential biomarkers and drug targets in asthma and provide the
possibility for development of novel diagnostic and therapeutical methods. 2. We are going to set an
in vivo method based on the technique of RNA interference, with which we will be able to study the
role of different genes in an in vivo animal model of asthma. With this method, we will be able to, at
least partly, substitute the expensive and time consuming gene knock out method. With the help of
this method it can be possible to study the functions and roles of genes detected in the SNP
screenings. 3. With the help of the optimized RNAi method, results of gene expression measurements
and SNP screens, it can be possible to assign new therapeutical (or even gene therapeutical) targets
in asthma. Working plan: 1.1 At present in our asthma biobank there are samples and clinical data
from 230 asthmatic children available for us, and we would like to increase this collection by 100 DNA
samples per annum. 1.2. The first genome area we are going to screen is the 11q13. Next we plan to
screen additional genome areas (e.g. 12q14.3-q24.31, 13q14, 14q11.2-q13, 17q11.2) as well as
carry out SNP screenings in candidate genes identified through literature screening. 1.3. The first task
in our RNAi experiments to verify the effectiveness of the intratracheal delivery by fluorescencelabeled non-gene specific control siRNA, and confirm that with this method the siRNA molecule gets
really to the lung as a target organ of the animal. Then with the help of our protocol we make the
mice producing symptoms similar to human asthma, and threat the animals with siRNA specific for
important asthma genes. With different methods (e.g. measurement of airway hyperreactivity, lung
histochemistry, investigation of BAL cells with FACS etc.) we study the effect of RNAi therapy to the
symptoms and parameters of the animals. Among our plans there is a high throughput gene
expression measurement in the Agilent Microarray Core Facility, also in the area of the institute. With
the help of gene expression chips we would like to follow the changes of the gene expression in the
lung of the animals throughout these processes. 1.4. We would like to utilize the results from the
whole genome gene expression microarray experiments in our candidate gene (pathway) association
studies, i.e. we compare the SNP patterns found in asthmatic patients in these genes with those
found in healthy controls.
Project Website
www.dgci.sote.hu
Contact Person
Name
DSc Csaba Szalai
Email
[email protected]
Genetics
Function
PI
Phone
361-210-2930/6502
Fax
361-303-6968
Website
www.dgci.sote.hu
Research Team Objectives
Main Fields
Genetics
Paediatrics
Pharmacology
Aim: 1. With the help of large number of asthmatic and healthy patients and SNP screening methods
we would like to identify genes and genetic variations playing roles in the pathomechanism of and
susceptibility to asthma. A Core Facility for high throughput SNP screening (GenomeLab SNPstream
with the capacity of several million SNPs in a day) is available for us, which provides the possibility to
identify unknown genes or genome areas in asthma. These genes and their protein products are
potential biomarkers and drug targets in asthma and provide the possibility for development of novel
diagnostic and therapeutical methods. 2. We are going to set an in vivo method based on the
technique of RNA interference, with which we will be able to study the role of different genes in an in
vivo animal model of asthma. With this method, we will be able to, at least partly, substitute the
expensive and time consuming gene knock out method. With the help of this method it can be
possible to study the functions and roles of genes detected in the SNP screenings. 3. With the help of
the optimized RNAi method, results of gene expression measurements and SNP screens, it can be
possible to assign new therapeutical (or even gene therapeutical) targets in asthma. Working plan:
1.1 At present in our asthma biobank there are samples and clinical data from 230 asthmatic children
available for us, and we would like to increase this collection by 100 DNA samples per annum. 1.2.
The first genome area we are going to screen is the 11q13. Next we plan to screen additional genome
areas (e.g. 12q14.3-q24.31, 13q14, 14q11.2-q13, 17q11.2) as well as carry out SNP screenings in
candidate genes identified through literature screening. 1.3. The first task in our RNAi experiments to
verify the effectiveness of the intratracheal delivery by fluorescence-labeled non-gene specific control
siRNA, and confirm that with this method the siRNA molecule gets really to the lung as a target organ
of the animal. Then with the help of our protocol we make the mice producing symptoms similar to
human asthma, and threat the animals with siRNA specific for important asthma genes. With different
methods (e.g. measurement of airway hyperreactivity, lung histochemistry, investigation of BAL cells
with FACS etc.) we study the effect of RNAi therapy to the symptoms and parameters of the animals.
Among our plans there is a high throughput gene expression measurement in the Agilent Microarray
Core Facility, also in the area of the institute. With the help of gene expression chips we would like to
follow the changes of the gene expression in the lung of the animals throughout these processes. 1.4.
We would like to utilize the results from the whole genome gene expression microarray experiments
in our candidate gene (pathway) association studies, i.e. we compare the SNP patterns found in
asthmatic patients in these genes with those found in healthy controls.
Partners/Interests
researcher
industrial partner
Genetics
Pharmacology
Paediatrics
We are looking for partner, who can participate in our partial genome screening project in asthma,
with additional asthmatic patients (children, or families), or bioinformatics who would be able to help
us to evaulate or SNP results. Or we are looking for partner, with whom we could cooperate in our
RNAi researches.
Asthma genetics and genomics Aim: 1. With the help of large number of asthmatic and healthy
patients and SNP screening methods we would like to identify genes and genetic variations playing
roles in the pathomechanism of and susceptibility to asthma. A Core Facility for high throughput SNP
screening (GenomeLab SNPstream with the capacity of several million SNPs in a day) is available for
us, which provides the possibility to identify unknown genes or genome areas in asthma. These genes
and their protein products are potential biomarkers and drug targets in asthma and provide the
possibility for development of novel diagnostic and therapeutical methods. 2. We are going to set an
in vivo method based on the technique of RNA interference, with which we will be able to study the
role of different genes in an in vivo animal model of asthma. With this method, we will be able to, at
least partly, substitute the expensive and time consuming gene knock out method. With the help of
this method it can be possible to study the functions and roles of genes detected in the SNP
screenings. 3. With the help of the optimized RNAi method, results of gene expression measurements
and SNP screens, it can be possible to assign new therapeutical (or even gene therapeutical) targets
in asthma. Working plan: 1.1 At present in our asthma biobank there are samples and clinical data
Genetics
from 230 asthmatic children available for us, and we would like to increase this collection by 100 DNA
samples per annum. 1.2. The first genome area we are going to screen is the 11q13. Next we plan to
screen additional genome areas (e.g. 12q14.3-q24.31, 13q14, 14q11.2-q13, 17q11.2) as well as
carry out SNP screenings in candidate genes identified through literature screening. 1.3. The first task
in our RNAi experiments to verify the effectiveness of the intratracheal delivery by fluorescencelabeled non-gene specific control siRNA, and confirm that with this method the siRNA molecule gets
really to the lung as a target organ of the animal. Then with the help of our protocol we make the
mice producing symptoms similar to human asthma, and threat the animals with siRNA specific for
important asthma genes. With different methods (e.g. measurement of airway hyperreactivity, lung
histochemistry, investigation of BAL cells with FACS etc.) we study the effect of RNAi therapy to the
symptoms and parameters of the animals. Among our plans there is a high throughput gene
expression measurement in the Agilent Microarray Core Facility, also in the area of the institute. With
the help of gene expression chips we would like to follow the changes of the gene expression in the
lung of the animals throughout these processes. 1.4. We would like to utilize the results from the
whole genome gene expression microarray experiments in our candidate gene (pathway) association
studies, i.e. we compare the SNP patterns found in asthmatic patients in these genes with those
found in healthy controls.
Institution/University
Name of Institution/University
Semmelweis University Department of Genetics Cell and Immunobi
Number of Employees
35
Number of Researchers
20
Country
Hungary
Postal Adress
Nagyvarad ter 4
1089 Budapest
Genetics
Research Team
Research Team Name
Bone and Soft Tissue Sarcomas & Bone Metastases
Individual Researcher
Yes
Research Team Leader Name
Prof. Dr. Andreas Leithner
Contact Person
Name
Prof. Dr. Andreas Leithner
Email
[email protected]
Function
Assistant Head and Chair of the Department of Orthopaedic Surgery
Phone
+43 316 385 81899
Fax
+43 316 385 4806
Website
www.meduni-graz.at/orthopaedie/
Research Team Objectives
Main Fields
Genetics
Pathology
Surgery
interdisciplinary research on bone and soft tissue sarcomas / bone and soft tissue metastases
Partners/Interests
researcher
Pathology
Surgery
Genetics
cooperation
scoring systems for bone metastases using biological markers
Institution/University
Name of Institution/University
Medical University Graz
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Austria
Postal Adress
Auenbruggerplatz 5
8036 Graz
Genetics
Research Team
Research Team Name
Forensic DNA laboratory, Institute of forensic Medicine
Individual Researcher
No
Research Team Leader Name
PhD Oliver Stojkovic
Research Team Members
Team Member 0
assistant professor Oliver Stojkovic [[email protected]]
Team Member 1
MD Aleksandar Stanojevic [[email protected]]
Team Member 3
DNA analyst Tatjana Varljen [[email protected]]
Research Team Projects
Project Name
Structure of genetic variation of microsatelite loci in ethnic
comunities in Serbia and Montenegro
Project Leader
PhD Oliver Stojkovic [[email protected]]
Project Funding Agency
Ministry of sciences Republic of Serbia
Project Budget
10.000 €
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Serbia and MOntenegro / University of Novi Sad / Institute of
Forensic Medicine Serbia and Montenegro / University of
Kragujevac / Institute of Pathology and Forensic Medicine Serbia
and Montenegro / University of Nis / School of Medicine, Division of
Forensic Medicine
Project Summary
Detailed understanding of spatial and ethnical organization of genetic variability in humans can
provide valuable information for genetic epidemiology (on the reasons of differential incidence of
certain diseases in various ethnical groups), as well as for anthropology and evolutionary biology (on
mechanisms that have shaped evolutionary history of our species, and demographic history of local
populations). In this project, we will genotype more than a 1000 human samples. Sampling will be
based on regional birthplace location and self reported ethnicity. Our previous studies were based on
the unstructured population from Serbia and Montenegro. In this project, we will take into account
the fact of historical and demographic heterogeneity of Serbia and Montenegro general population, so
the observed genetic variability will be partitioned into its ethnical and spatial component, by
population genetic statistics (AMOVA, F statistics, D statistics, gene genealogies). Considering that
our community has recently passed through several consecutive bottleneck episodes, in both world
wars, we will be able to test if these historical events left marks on genetic structure of our
community. Knowledge of geographical and ethnical origin of tested subjects, alone and with regard
to data from other European populations, will allow us to describe and determine patterns of spatial
genetic variation, migration, gene flow, population subdivision and population level medical
associations.
Project Website
www.med.bg.ac.yu
Contact Person
Name
PhD Oliver Stojkovic
Email
[email protected]
Function
Head of DNA laboratory
Phone
+381113617931
Fax
+381-11-3617931
Website
www.med.bg.ac.yu
Research Team Objectives
Main Fields
Genetics
Biology
molecular anthropology, Forensic genetics, DNA typing, human population genetics, ancient DNA,
degraded DNA
Genetics
Partners/Interests
researcher
Genetics
Biology
Access to DNA samples or the ability to collect DNA samples from ethnical groups involved in the
future research.
Population genetics studies in ethnical groups living in the Southern Europe area, such as Roman
(Gypsies) populations
Institution/University
Name of Institution/University
University of Belgrade
Number of Employees
10000
Number of Researchers
5000
Country
Serbia and Montenegro
Postal Adress
Studentski trg 16
11000 Belgrade
Genetics
Research Team
Research Team Name
Genetic epidemiology and pharmacogenomics
Individual Researcher
No
Research Team Leader Name
Dr Dragan Alavantic
Research Team Members
Team Member 0
PhD Dragan Alavantic [[email protected]]
Team Member 1
PhD Olga Jozanov-Stankov [[email protected]]
Team Member 2
PhD Aleksandra Stankovic [[email protected]]
Team Member 3
PhD Maja Zivkovic [[email protected]]
Team Member 4
Mr Sci Tamara Djuric [[email protected]]
Team Member 5
Bs Sci Sanja Mecanin [[email protected]]
Team Member 6
Bs Sci Aleksandar Rakovic [[email protected]]
Team Member 7
Bs Sci Olja Stancic [[email protected]]
Research Team Projects
Project Name
Genetic epidemiology and pharmacogenomics of vascular
diseases
Project Leader
Dr Dragan Alavantic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Project Summary
Research are focused on investigation of primary gene structure in healthy subjects and in groups of
patients with vascular diseases (cardiovascular, cerebrovascular, renovascular) and certain
neurodegenerative diseases. Objects are key genes involved in lipid metabolism, regulation of blood
pressure, extracellular matrix remodeling, inflammation and oxidative stress. The structure and
population specificity of genes for: apolipoproteins (A,B,C,E); LPL, LDLR, CETP, PPaR(a,g), ENPP1,
RAS (ACE,ACE2, AT1R,AT2R,AGT), MMPs, and proteins involved in inflammatory cascade and
oxidative stress (IL-6, MCP-1, IL-1beta, IL-1ra, NOS-3, GPx4,flGST,PON1) by establishing
(1)genotype and allele frequency of the genes in both healthy population and different groups of
patients, (2)haplotype effects as well as risk factors on: gene expression, phenotype, and chosen
therapy. The goal-a new knowledge obtained by investigation of primary gene structure in population
of Serbia, which should be stimulating for introducing pharmacogenetics in clinical practice. Research
goal in the first two years of the project is, by determination of the relationship between primary
structure and expression of the genes, taking into consideration risk factors, to define persons at the
highest risk. Well defined predictive factors should be then made possible for an investigation of
proper diagnostics, prevention and therapy certain groups of patients which suffered from the most
frequent diseases in Serbia
Project Website
mntr.sr.gov.yu
Contact Person
Name
Dr Dragan Alavantic
Email
[email protected]
Function
Head of project
Phone
+381 11 2447485
Fax
+38 11 2447485
Website
vin.bg.ac.yu
Research Team Objectives
Main Fields
Genetics
Epidemiology
Clinical chemistry
Genetics
Gene candidate DNA polymorphisms and their association with vascular diseases, main risk factors
(dislipidaemia, hypertension, diabetes, smoking ..) as well as certain renal and neurodegenerative
disease.. Population (case-control) and family studies, both genotype and allele frequencies, genegene and gene-environment relationship, haplotyping, development of methods for clinical use.
Partners/Interests
researcher
Genetics
Clinical chemistry
Epidemiology
from institutes and clinics
Genetic epidemiology of stroke Genetics of oxidative stress
Institution/University
Name of Institution/University
VINCA Institute of Nuclear Sciences
Number of Employees
750
Number of Researchers
400
Country
Serbia and Montenegro
Postal Adress
POBox 522 522
11001 Belgrade
Genetics
Research Team
Research Team Name
Human genetics and pharmacogenomics
Individual Researcher
Yes
Research Team Leader Name
Assistant Prof., PhD Uroš Potočnik
Research Team Projects
Project Name
Genetic susceptibility to gastrointestinal complex diseases
and pharmacogenomics
Project Leader
Assistant Prof., PhD Uroš Potočnik [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2005-09-01
Project End Date
2008-08-31
Project Partners
1. Slovenia/University of Maribor, Faculty of Electrical Engineering
and Computer Science/Laboratory for system design 2.
Slovenia/University of Ljubljana, Faculty of Medicine/Department
for molecular genetics 3. Slovenia/University of Ljubljana, Faculty
of Medicine/Institute for microbiology 4. Slovenia/Taeching Hospital
Maribor/Medical departments
Project Summary
The aim of our study is to provide new molecular diagnostic markers and molecular targets for novel
drugs design for better prevention and treatment of complex gastrointestinal diseases.
Project Website
http://sicris.izum.si/search/prj.aspx?opt=3&l
Project Name
Molecular genetic and pharmacogenomic markers in
complex gastrointestinal diseases
Project Leader
Assistant Prof., PhD Uroš Potočnik [[email protected]]
Project Funding Agency
ARRS & NIH
Project Budget
25000 €
Project Start Date
2006-03-01
Project End Date
2008-02-28
Project Partners
1. USA/ National Institutes of Health, National Cancer
Institute/Laboratory of genomic diversity 2. Slovenia/University of
Maribor, Faculty of Electrical Engineering and Computer
Science/Laboratory for system design 3. Slovenia/University of
Ljubljana, Faculty of Medicine/Department for molecular genetics
Project Summary
Particularly focus of our study is to provide new molecular targets and molecular diagnostic markers
including Single nucleotide polymorphisms (SNPs), haplotypes and expression profiles, for novel
drugs design for better prevention and treatment of complex gastrointestinal diseases.
Project Website
under construction
Contact Person
Name
Assistant Prof., PhD Uroš Potočnik
Email
[email protected]
Function
Head of Centre for human genetics and pharmacogenomics
Phone
+ 386 2 234 56 01
Fax
+ 386 2 23 45 600
Website
www.mf.uni-mb.si
Research Team Objectives
Main Fields
Genetics
Human genetics and pharmacogenomics
Genetics
Partners/Interests
researcher
industrial partner
Suitable
Genetics
Biochemistry
Microbiology
1. SUSCEPTIBILITY GENES AND PHARMACOGENOMIC MARKERS IN COMPLEX DISEASES The aim of
our study is to identify genes, single nucleotide polymorphisms (SNPs) and haplotypes associated
with complex diseases including both major forms of inflammatory bowel diseases (IBD), ulcerative
colitis and Crohn disease, IBD-associated neoplasia (IBDNs), colorectal cancer (CRC), asthma, aspirin
intolerance, diabetes, cardio-vascular diseases, schizophrenia. We will establish a comprehensive
follow-up patients database and bioinformatic tools for finding statistical correlations between
molecular genetic and clinicopathological data including treatment response. We will also determine
the gene expression profiles in blood leucocytes and colon biopsies taken from IBD patients during
standard treatment with corticosteroids and immunosuppresives and treatment with monoclonal
antibody inhibitor against TNF alfa (Inflaximab) and correlate results with treatment response. We will
use combination of different approaches in our disease association and pharmacogenomic studies
including candidate gene approach, genome-wide haplotype and linkage disequilibrium association
approaches and disease pathway analysis to characterize genetic risk factors predisposing to complex
diseases and treatment response. Our particular focus will be on identification of functional SNPs in
regulatory cis-acting regions and describing their role in differential gene expression and disease
association. We will develop new approach for identification of functional SNPs in regulatory cis-acting
regions based on allele specific expression in lymphoblastoid cell lines from CEPH families and
segregation analysis. The database with genes showing most significant allele specific expression will
be used for our disease association study and will be available on the internet to scientific community
for other disease association studies. Identified SNPs in regulatory cis-acting regions will be used for
identification of transcription factors. Alele specific expression will be compared between cell lines and
different tissues including colon, IBDNs, colon adenomas and colorectal tumors. The alternative splice
variants expressed in different tissues and pathogenic states will be described. We will also describe
molecular alterations, including somatic mutations, methylation status and global gene expression
profile in adenomas from IBDN patients, tumors from CRC patients and biopsies from IBD patients
during treatment. We will correlate genetic data with CRC patients prognosis and survival. We will
functionally characterize genes and proteins most significantly associated with disease. We will
develop new bioinformatic and statistical genetic tools for best candidate gene selection..We will use
oligo-microarrays, quantitative real time PCR (Taqman) and imunohistochemistry for expression
profiling. We will use gel-mobility shift assay for identification of transcription factors associated with
SNPs in regulatory regions.
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Genetics
Research Team
Research Team Name
Laboratory for Human Genetics, INGEB
Individual Researcher
No
Research Team Leader Name
Msc Lejla Kapur
Research Team Members
Team Member 0
Mrs Drazenka Macic [[email protected]]
Team Member 1
Mr Jasmin Ramic [[email protected]]
Team Member 2
Mrs Naida Lojo-Kadric [[email protected]]
Team Member 3
Dr Naris Pojskic [[email protected]]
Team Member 4
MSc Lejla Kapur [[email protected]]
Research Team Projects
Project Name
establishment of research basis for genetic characterization
of common cancers in B&H
Project Leader
prof dr Nermina Obralic [[email protected]]
Project Funding Agency
Ministry of Science, Sarajevo Canton
Project Budget
10000 €
Project Start Date
2004-07-31
Project End Date
2006-07-31
Project Partners
Bosnia and Herzegovina, Institute for Genetic Engineering and
Biotechnology, Sarajevo / Laboratory for Human genetics Bosnia
and Herzegovina, University Clinical Centre, Institute of Oncology
Bosnia and Herzegovina, University Clinical Centre, Institute of
Pathology
Project Summary
Overall aim of this project is to establish research basis and biobank for future molecular-genetic
research of etiology and farmacotherapeutic response in several common cancers in Bosnia and
Herzegovina. Main activities are related to optimization and validization of protocols for collection,
transport, DNA and RNA isolation, preservation and archiving of tumor tissue specimens. All relevant
data for patients (general, clinical, familial and therapeutically-related) are gathered into an electronic
biodatabase that is linked to genomic databank. Complete archive will serve in future genotypephenotype correlation studies. Currently it contains completed data for about a hundred specimens of
cancerous and precancerous tissue from breast, ovary and colon.
Project Website
Project Name
Identification of DNA markers for predisposing factors of
schizophrenia in patients from B&H;
Project Leader
MSc Lejla Kapur [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2004-12-01
Project End Date
2007-12-01
Project Partners
Bosnia and Herzegovina, Institute for Genetic Engineering and
Biotechnology, Sarajevo, Laboratory for Human genetics Bosnia
and Herzegovina, University Clinical Center Sarajevo, Psychiatric
Clinic Bosnia and Herzegovina, Institute for Genetic Engineering
and Biotechnology, Sarajevo, Laboratory for Bioinformatics and
Biostatistics
Project Summary
Case control study was performed to assess allelic, genotype and haplotype analysis of ten genomewide polymorphic markers with shizophrenia in cohort of 104 individuals: age, sex and ethnically
matched. Standard genotyping methodology, PCR-RFLP and microsatelite analysis was used.
Extensive population genetics analysis was performed using genotype an phenotype data.
Project Website
Genetics
Contact Person
Name
Lejla Kapur
Email
[email protected]
Function
Scientific Secretary
Phone
+38733220926,215778
Fax
+38733442891
Website
ingeb.ba
Research Team Objectives
Main Fields
Genetics
Information Technology, Statistics, Documentation
gene expression and structural analysis in common cancers; psychiatric genetics
Partners/Interests
researcher
Genetics
Epidemiology
access to patients expertise in one of listed fields (stages of project): 1. patients recruitment using
operationalised research criteria (for selected disorder) and general, clinical and therapeutic data
collection; 2. DNA, RNA, protein isolation, preservation and storage 3. one of bioogical level analysis
(genomic, transcription or expression) 4. extensive biostatistical analysis of relevant biological data
Regional Network for Genetics of Complex Disorders (Cancers and Neurodegenerative ilnesses)
Institution/University
Name of Institution/University
Institute for Genetic Engineering and Biotechnology
Number of Employees
20
Number of Researchers
16
Country
Bosnia and Herzegovina
Postal Adress
Kemalbegova 10
71000 Sarajevo
Genetics
Research Team
Research Team Name
Molecular basis of atherogenesis research team
Individual Researcher
No
Research Team Leader Name
PhD Goran Ferencak
Research Team Members
Team Member 0
BSc Natalija Marinkovic [[email protected]]
Team Member 1
Assistant Professor Ksenija Vitale [[email protected]]
Team Member 2
PhD Ljubica Vranic Stavljenic []
Team Member 3
PhD Branka Grskovic [[email protected]]
Team Member 4
Professor Ana Stavljenic Rukavina [[email protected]]
Team Member 5
PhD Daria Pasalic [[email protected]]
Team Member 6
PhD Jasna Lenicek-Krleza [[email protected]]
Team Member 7
MSc Ana Bronic [[email protected]]
Team Member 8
PhD Lorena Honovic [[email protected]]
Team Member 9
Assistant Professor Robert Bernat [[email protected]]
Research Team Projects
Project Name
Molecular basis of atherogenesis
Project Leader
PhD Goran Ferencak [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-10-01
Project End Date
2011-10-01
Project Partners
USA/Roche Molecular Systems Inc./Institute for Human Genetics
Project Summary
Atherosclerotic process which underlies pathobiochemical basis of cardiovascular diseases is
determined by a number of environmental and hereditary factors. The recognition of these factors is
crucial for medical intervention in patients, but also for primary prevention. More than one
biochemical pathway is included in the pathogenesis of atherosclerosis. Genes encoding various
proteins within these biochemical pathways are candidate genes for premature atherosclerosis
susceptibility markers. Included in this project research are candidate gene polymorphisms not
studied in previous research no. 108247: genes coding for molecules involved in the inflammatory
component of atherosclerosis and genes which may influence the concentration of triglycerides
(apolipoprotein A5 gene; fatty acid binding protein, FABP-2; toll-like receptor gene, TLR-2; plateletendothelial cell adhesion molecule gene, PECAM-1; interleukin 6 and 12 genes, IL-6 and IL-12;
monocyte chemoattractant protein 1 gene, MCP-1 and gene for its receptor CCR2). The second phase
of the project is dealing with influence of previously genotyped polymorphisms on clinical outcome in
patients after stenting. Previous research has shown that CETP (cholesteryl-ester transfer protein)
gene polymorphisms influence the risk for coronary heart disease and concentrations of HDL and
apolipoprotein A-I. Therefore, in the new reasearch the influence of different concentrations of mildly
and completely oxidized LDL in the presence of antisense CETP-oligonucleotides in a range of
concentrations on CETP gene expression and mass will be studied in HepG2 and mononuclear cells
from peripheral blood in cell culture. Since environmental factors significantly influence
atherogenesis, it is presumed that there is a difference in risk for atherosclerosis development
regarding the present xenobiotics and it will be evaluated in subjects from general population in
Koprivnièko-križevaèka county. If there is a difference in incidence of atherosclerosis between
exposed and non-exposed population, it is possible that polymorphisms in genes involved in
biotransformation of xenobiotics (cytochrome P450 1A1, CYP1A1, and multidrug resistance gene,
MDR1) are associated with different risks for atherosclerosis development.
Project Website
Contact Person
Name
PhD Goran Ferencak
Email
[email protected]
Genetics
Function
Clinical chemistry and laboratory medicine specialist
Phone
+38598411475
Fax
+38514590236
Website
Research Team Objectives
Main Fields
Genetics
Clinical chemistry
Epidemiology
Atherosclerotic process which underlies pathobiochemical basis of cardiovascular diseases is
determined by a number of environmental and hereditary factors. The recognition of these factors is
crucial for medical intervention in patients, but also for primary prevention. More than one
biochemical pathway is included in the pathogenesis of atherosclerosis. Genes encoding various
proteins within these biochemical pathways are candidate genes for premature atherosclerosis
susceptibility markers. Included in this project research are candidate gene polymorphisms not
studied in previous research no. 108247: genes coding for molecules involved in the inflammatory
component of atherosclerosis and genes which may influence the concentration of triglycerides
(apolipoprotein A5 gene; fatty acid binding protein, FABP-2; toll-like receptor gene, TLR-2; plateletendothelial cell adhesion molecule gene, PECAM-1; interleukin 6 and 12 genes, IL-6 and IL-12;
monocyte chemoattractant protein 1 gene, MCP-1 and gene for its receptor CCR2). The second phase
of the project is dealing with influence of previously genotyped polymorphisms on clinical outcome in
patients after stenting. Previous research has shown that CETP (cholesteryl-ester transfer protein)
gene polymorphisms influence the risk for coronary heart disease and concentrations of HDL and
apolipoprotein A-I. Therefore, in the new reasearch the influence of different concentrations of mildly
and completely oxidized LDL in the presence of antisense CETP-oligonucleotides in a range of
concentrations on CETP gene expression and mass will be studied in HepG2 and mononuclear cells
from peripheral blood in cell culture. Since environmental factors significantly influence
atherogenesis, it is presumed that there is a difference in risk for atherosclerosis development
regarding the present xenobiotics and it will be evaluated in subjects from general population in
Koprivnièko-križevaèka county. If there is a difference in incidence of atherosclerosis between
exposed and non-exposed population, it is possible that polymorphisms in genes involved in
biotransformation of xenobiotics (cytochrome P450 1A1, CYP1A1, and multidrug resistance gene,
MDR1) are associated with different risks for atherosclerosis development.
Partners/Interests
researcher
industrial partner
Genetics
Clinical chemistry
Epidemiology
Influence of newly developed drugs on atherogenic mechanisms (in cell culture) Influence of
genotypes in atherosclerosis candidate genes on efficiency of newly developed drugs
Institution/University
Name of Institution/University
Zagreb University School of Medicine
Number of Employees
700
Number of Researchers
100
Country
Croatia
Postal Adress
Salata 3
10000 Zagreb
Genetics
Research Team
Research Team Name
Oral Biology Group
Individual Researcher
No
Research Team Leader Name
PhD, professor Jelena Milasin
Research Team Members
Team Member 0
assistant Gavrilo Brajovic [[email protected]]
Team Member 1
assistant-professor Dusan Pavlica []
Team Member 2
assistant Ana Pucar [[email protected]]
Team Member 3
assistant Branka Popovic [[email protected]]
Contact Person
Name
PhD, professor Jelena Milasin
Email
[email protected]
Function
Vice-dean
Phone
+381 11 2685 288
Fax
+381 11 2685 361
Website
stomf.bg.ac.yu
Research Team Objectives
Main Fields
Genetics
Microbiology
Dentristry
Main research fields: 1) molecular genetics of oral malignancies and premalignant lesions of the head
and neck region; 2) etiopathogenesis of periodontal disease
Partners/Interests
researcher
Microbiology
Genetics
Dentristry
competencies in molecular biology and microscopy (electron and laser)
Establishing the connection between periodontal disease and atherosclerosis. Comparative study of
periodontopathogens present in dental pockets and atherosclerotic vessels in patients undergoing
vascular surgery.
Institution/University
Name of Institution/University
School of Dentistry, University of Belgrade
Number of Employees
440
Number of Researchers
180
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgrade
Genetics
Research Team
Research Team Name
polymorphism in orthopaedics research team
Individual Researcher
No
Research Team Leader Name
Dr. Heimo Clar
Research Team Members
Team Member 0
Ass. Dr. Gerald Gruber [[email protected]]
Team Member 1
Prof. Dr. Andreas Leithner [[email protected]]
Team Member 2
Ass. Dr. Heimo Clar [[email protected]]
Research Team Projects
Project Name
polymorphisms in orthopaedics
Project Leader
dr heimo clar [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-05-01
Project End Date
2010-05-01
Project Partners
Project Summary
creating a genetic data base and doing relevant research in bone and joint metabolism and diseases
Project Website
Contact Person
Name
dr. heimo clar
Email
[email protected]
Function
ass.
Phone
+43 316 385 80484
Fax
+43 316 385 2957
Website
meduni-graz.at
Research Team Objectives
Main Fields
Genetics
Microbiology
Surgery
polymorphisms could be relevant in the understanding and treatment of orthopaedic diseases.
Partners/Interests
researcher
industrial partner
Microbiology
Genetics
Surgery
interest in an cooperation for basic research in bone and joint metabolsm and diseases
finding research partners
Institution/University
Name of Institution/University
department of orthopaedic surgery, medical university of graz
Number of Employees
17
Number of Researchers
15
Country
Austria
Postal Adress
Auenbruggerplatz 5-7
8036 graz
Genetics
Research Team
Research Team Name
Translational Medicine Group
Individual Researcher
No
Research Team Leader Name
dr Oliver Vugrek
Research Team Members
Team Member 0
Dr Oliver Vugrek [[email protected]]
Team Member 1
dipl. ing Robert Beluzic [[email protected]]
Research Team Projects
Project Name
S-Adenosylhomocysteine hydrolase deficiency: Molecular
mechanisms of a new disease in human
Project Leader
Dr Oliver Vugrek [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2007-01-01
Project End Date
2009-12-31
Project Partners
Project Summary
The molecular basis for SAHH deficiency has not yet been resolved. DNA analysis of individuals with
extremely high levels of SAH showed a total of 5 different not yet characterized point mutations in the
SAHH gene: two point mutations, a premature stop codon and a missense mutation in 2 infants (Baric
et al, 2004, 2005), two missense mutations in an adult with severe mental retardation (Buist et al
2006), and two missense mutations in a newborn causing death at the age of 4 month (unpublished
data). Additionaly, we have linked another polymorphism in the SAHH gene (Gellekink et al. 2004) to
three phenotypically yet inconspicuous individuals (unpublished data).To elucidate the impact of these
particular point mutations on the enzymatic activity of SAHH, we constructed expression vectors and
analyzed the biochemical properties of recombinant S-Adenosylhomocysteine hydrolase. The bestcharacterized missense mutation so far is a tyrosine to cysteine exchange (p.Y143C), which
represents a temperature sensitive mutation. Namely, heterologous expression of mutant protein at
permissive temperature results in protein with reduced enzymatic activity compared to wildtype,
whereas expression at restrictive temperature yields in completely nonfunctional protein. Circular
dichroism analysis of the p.Y143C mutant protein showed a significantly reduced unfolding
temperature of 7°C if compared to wildtype protein. Accordingly, dynamic light scattering
experiments showed accellerated aggregation of mutant protein at rising temperature. Native
polyacrylamide gel electrophoresis showed, that mutant p.Y143C SAHH subunits are able to form the
tetrameric complex as is the wildtype enzyme. Nevertheless, the aminoacid exchange of tyrosine to
cysteine probably leads to the loss of a hydrogen bond with glutamic acid 115, which might be
essential for proper subunit folding or substrate binding. The catalytic rates in direction of SAH
hydrolysis or synthesis are decreased to approx. 30% if compared to recombinant wildtype SAHH. Km
values of mutant protein are decreased accordingly. Quantitative binding of co-factor NAD is not
affected in p.Y143C, but there is a dramatic change in the oxidation states, i.e. mutant protein
contains mainly NADH (80%), whereas wildtype protein contains approx. 90% NAD+. The NADH
accumulation indicates that protons involved in the oxidation reactions are leaking from the active
site rather than being retained as is the case for wildtype SAHH.
Project Website
www.irb.hr/korisnici/~ovugrek/home1.htm
Contact Person
Name
dr Oliver Vugrek
Email
[email protected]
Function
Research associate
Phone
+384-1-4560946
Fax
+385-1-4680119
Website
http://www.irb.hr/korisnici/ovugrek/home1.htm
Genetics
Research Team Objectives
Main Fields
Genetics
Biochemistry
Biology
S-adenosylhomocysteine
hydrolase
(SAHH)
catalyzes
the
reversible
hydrolysis
of
Sadenosylhomocysteine (SAH) to adenosine (Ado) and L-homocysteine (Hcy). In eukaryotes, this is
the major route for disposal of the SAH formed as a product of many S-adenosylmethionine (SAM)dependent methyltransferases. Thus, SAH hydrolysis is believed to play a critical role in the regulation
of biological methylation, which on the other hand is important for post-translational modifications.
Accordingly, protein methylation is important for protein-protein interactions, cellular localization and
maturation of heterogeneous ribonucleoproteins and it might be crucial in cellular signaling and viral
replication. Also, methylation is an important part of the histone code that regulates cell type–specific
gene expression programs. Interestingly, aberrant methylation is a widespread phenomenon in
cancer and may be among the earliest changes during oncogenesis.Aberrant methylation processes
might be the reason for the severe pathologic effect of the new genetic disorder of SAHH deficiency in
human. Namely, SAHH deficiency results in an intracellular accumulation of SAH (150-fold) and SAM
(30-fold), causing a significant imbalance in the intracellular SAH/SAM ratio in patients. SAH is a
strong competitive inhibitor of many SAM-dependent methyltransferases. Therefore, the significant
imbalance in the SAM/SAH ratio in SAHH deficient patients might be the reason for other
abnormalities such as myopathy, retarded psychomotor development and signs of mental retardation
(Baric et al, 2004). Further, bioinformatics results from the human genome project show that there
exist a large number of methyltransferases, but their substrates have not been characterised yet.
Methylation may thus be far more important than previously thought, which is consistent with the
severe pathologic effect of SAHH deficiency. The molecular basis for SAHH deficiency has not yet
been resolved. DNA analysis of individuals with extremely high levels of SAH showed a total of 5
different not yet characterized point mutations in the SAHH gene. To elucidate the impact of these
particular point mutations on the enzymatic activity of SAHH, we constructed plasmid expression
vectors for a detailed analysis of the biochemical properties of mutated recombinant SAHH. The
functional analysis will allow conclusions about the involvement of the identified mutations in protein
activity and herein serve as basis for our hypothesis that irregular methylation processes caused by
inactivation of S-Adenosylhomocysteine hydrolase are the reason for the severe pathologic effect of
the new genetic disorder of SAHH deficiency in human.
Partners/Interests
researcher
industrial partner
Biochemistry
Genetics
Clinical chemistry
Anything which suits our research area described under project ideas etc.
We have outlined two major research objectives, which can be divided into a functional genomics
section and a proteomics section. Functional genomics will allow insights on the impact of 5 distinct
point mutations on structure and function of S-adenosylhomocysteine hydrolase. Namely, several
individuals with extremely high levels of SAH share not yet characterized point mutations in the SAHH
gene. The severeness of the disease is variable and causes serious health problems with a possible
lethal outcome if some of the point mutations occur in combination. To elucidate the impact of each
of the point mutations on the enzymatic activity of SAHH, we will produce recombinant protein for
functional analysis. Using plasmid expression vectors and heterologous expression in bacteria we
have identified a temperature sensitive mutation. Accordingly, we will be analysing the remaining 4
point mutations in the SAHH gene to explain the mechanisms which underlie enzyme inactivation. We
have established biochemical assays for measuring enzyme activity and co-factor NAD+/NADH
content. Methods such as circular dichroism analysis and dynamic light shattering will be used for
questioning temperature dependent behaviour of mutant protein. We will perform gene expression
studies on DNA and RNA extracted from the fibroblast cell line obtained from patients. The
thermosensitive nature of one of the mutation allows an experimental design, which uses cultured
cells grown at different temperatures, i.e. permissive and restrictive temperature. DNA microarray
analysis will be performed by using the most comprehensive human genome microarray, which
contains complete coverage for the analysis of over 47,000 transcripts. Data analysis is performed by
computational tools and software provided by the manufacturer of the microarrays. Differences in
expression profiles are catalogued and used to build pathways to cellular processes involving SAHH
and its role in methylation processes or their regulation. The inhibitory effects of elevated intracellular
SAH concentrations on viral mRNA cap-methylating enzymes will be investigated. Replication of
human Cytomegalovirus in cultured fibroblasts will be monitored by the use of GFP labeled
recombinant virus. We will be able to give answers, whether viral replication is affected by SAHH
deficiency, because of irregular methylation processes. This could lead to new insights for
Genetics
mechanisms and necessary cellular factors, which are driving viral replication. Imprinting analysis will
be performed by the bisulfite modification of DNA and subsequent PCR. Resulting PCR products are
either analyzed by DNA sequencing or restriction digestion. The methylation status of the DNA
segment is then determined by comparing the bisulfite treated DNA and untreated DNA. Proteomics
will be monitoring changes at subcellular level such as posttranslational modification of the proteome
with focus on biological methylation. Accordingly, our established model system based on cultured
fibroblasts of patients allows comparison of DNA, RNA and protein modifications of cells cultured
under different growth conditions as mentioned above for the genomics part. Two-dimensional (2-D)
gelelectrophoresis and subsequent mass spectrometry is used to detect proteins with altered
methylation profiles. Accordingly, protein extracts of cultured fibroblasts from a SAHH deficient
patient, which have been grown at different temperatures will be compared and screened for
differences in the proteome. Thus our goal will be the identification of differences in quantity of
methylation sites between permissive and restrictive growth conditions using heavy methyl SILAC
(stable isotope labeling by amino acids in cell culture). Namely, radioactively labeled methyl groups
differ in their molecular weight if compared with nonradioactive methyl groups, which can be resolved
through mass spectrometry. Detection of alteration by either SILAC or mass spectrometry indicates,
that altered proteins are functionally connected through common pathways. Bioinformatics will thus
lead to the uncovering of possibly unknown pathways of cellular processes involved in biological
methylations. Further, identification of proteins with altered methylation will allow conclusions about
the activity of enzymes involved in methylation processes such as methyltransferases. New emerging
technologies such as proteome microarrays will be used to screen for proteins, which have lost their
antigenicity because of loss of post-translational modification such as methyl groups. Also, we will be
using classical western blot detection techniques with methylation-sensitive antibodies, which
recognise methyl groups on the protein surface. Newly identified proteins with altered methylation
profiles will be cloned using recombinant DNA technology and functionally analysed. We expect to
identify yet unknown methyl acceptors, i.e. substrates of methyltransferases, which are involved in
the complex pathogenesis of SAHH deficiency in human.
Institution/University
Name of Institution/University
Institute Rudjer Boskovic
Number of Employees
700
Number of Researchers
300
Country
Croatia
Postal Adress
Bijenicka 54
10000 Zagreb
Immunology and Immunohaematology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
prof. Milan Taradi
Contact Person
Name
prof. Milan Taradi
Email
[email protected]
Function
Professor of Physiology and Immunology
Phone
38514566921
Fax
38514590207
Website
web.mef.hr
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Information Technology, Statistics, Documentation
Biology
Tumour immunology Experimental allergic encephalomyelitis in rats E-learning
Partners/Interests
researcher
Tumour immunology
Immunology and Immunohaematology
Institution/University
Name of Institution/University
University of Zagreb, Faculty of Medicine
Number of Employees
800
Number of Researchers
500
Country
Croatia
Postal Adress
Šalata 3
10000 Zagreb
Immunology and Immunohaematology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
MD, PHD Gordana Leposavic
Research Team Members
Team Member 0
BSc. Nataša Kuštrimović [[email protected]]
Team Member 1
BSc. Katarina Mitić [[email protected]]
Team Member 2
MSc. Ana Rakin [[email protected]]
Team Member 3
BSc. Milica Perišić [[email protected]]
Team Member 4
BSc. Ivan Pilipović [[email protected]]
Team Member 5
MD Katarina Radojević [[email protected]]
Team Member 6
PhD Nevena Arsenović-Ranin [[email protected]]
Team Member 7
MD Vesna Kovačević-Jovanović [[email protected]]
Team Member 8
PhD Mileva Mićić [[email protected]]
Team Member 9
PhD Stanislava Stanojević [[email protected]]
Team Member 10
PhD Duško Kosec [[email protected]]
Team Member 11
PhD Bosiljka Plećaš-Solarović [[email protected]]
Team Member 12
PhD Vesna Vujuć [[email protected]]
Team Member 13
PhD Mirjana Dimitrijević [[email protected]]
Research Team Projects
Project Name
Neuroendocrine immunomodulation: roles for the sympathoadrenomedullary system nomedullary system
Project Leader
Professor of Pathological Physiology Gordana Leposavic
[[email protected]]
Project Funding Agency
Ministry of Science Republic of Serbia
Project Budget
20000 €
Project Start Date
2006-01-01
Project End Date
2010-01-01
Project Partners
Immunology Research Center “Branislav Jankovic”, Institute of
Immunology and Virology, Belgrade, Serbia Department of Medical
Chemistry, School of Medicine, University Belgrade, Belgrade,
Serbia
Project Summary
The sympatho-adrenomedullary (SA) system is thought to play an important part in the modulation of
immune functions, particularly in aging individuals and conditions of stress. This project aims to
enlighten the molecular and cellular mechanisms by which catecholamines (CAs) and neuropeptide Y
(NPY, a co-transmitter in noradrenergic sympathetic nervous fibers) contribute to:1) the maintenance
of immune homeostasis, and 2) the development of age- and stress-related alterations in the immune
functions. The investigations will be focused on role for CAs and NPY in modulation of: a) T-cell
development/ functions and b) macrophage activity in young, adult and aged rats. The study will
encompass pharmacological manipulations of CA action in vivo and well-defined models of chronic
stress. Changes in SA - immune communication during ageing and in models of chronic stress will be
assessed by analyzing the concentrations of CA and NPY in blood and lymphoid organs/ immune cells,
and the expression of their receptors on immune cells. To examine the direct effects of CAs, alone
and in combination with NPY, on the target cells, agonists and antagonists of adrenoceptors (ARs)
and/ or receptors for NPY will be used in thymus organ and lymphocyte/ macrophage cell cultures.
The investigations will be carried out in the absence and presence of glucocorticoid (GC) action to
address the significance of GCs in the effects of CAs and NPY on immune cells in vivo and in vitro.
Contact Person
Name
MD, PHD Gordana Leposavic
Email
[email protected]
Immunology and Immunohaematology
Function
Professor of Pathophysiology
Phone
+381 11 467 465
Fax
+381 11 467 465
Website
www.pharmacy.bg.ac.yu
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Pathology
Pharmacology
Neuroendocrine-immune system interactions, T-cell differentiation/ maturation, macrophage/
neutrophil functions, inflammation, mechanisms of autoimmunity, development and aging of the
immune system
Partners/Interests
researcher
Immunology and Immunohaematology
Pathology
Pharmacology
We are looking for a partner with experience in: a) in studies of the thymocyte selection and
maturation, b) establishing various models of autoimmune diseases c) molecular techniques that will
allow to define the impact of catecholamines / NPY on transcription and protein expression of
multiple, immunologically relevant genes.
I Catecholamines and thymocyte selection/maturation: ad 1. As we have shown that long-lasting
beta-adrenoceptor (AR) blockade in rats affects development of both conventional and regulatory T
cells leading to an intrathymic overrepresentation of the most mature single positive (CD4+8- and
CD4-8+) TCRalpha/betahigh cells and CD4+25+ T regulatory cells (Mol. Cell. Biochem., 285:87-99,
2006), most likely, by influencing the thymocyte selection (possibly via action on Thy-1 surface
expression), we are interested in continuing investigations of relationship between the effects of
catecholamine (CA) and TCR ligand binding in T-cell selection and maturation. ad 2. Having in mind
age-associated changes in the T-cell development, particularly those related to a pronounced
decrease in the thymocyte Thy-1 surface density that we have observed in rats (Exp.
Gerontol.41:574-589, 2006), on the one hand, and those in the sympathetic innervation of the rats
thymus, on the other hand, we would like to undertake further studies on relationship between the
effects of CA and TCR ligand binding in T-cell selection and maturation during the immune system
ageing. ad 3. Since we have observed that intrathymic CA concentration is substantially influenced by
gonadal steroid hormones (Neuroimmunomodulation, 7: 59-67, 2000, and our unpublished results),
we are planning to examine relationship among the effects of CA and TCR ligand binding in T-cell
selection and maturation in respect to sexual dimorphism, i.e. gonadal hormone presence. II
Catecholamines in pathogenesis of autoimmune diseases: As we have demonstrated that long-lasting
beta-AR blockade causes profound changes in susceptibility of DA rats to the induction of EAE, as well
as in the clinical course of disease (Immunol. Letters, 73: 221, 2000), we are interested in
undertaking a study to define the cellular and molecular targets underlining CA role in pathogenesis
of autoimmune diseases. III NPY and catecholamines in modulation of macrophage functions: ad 1. In
a view of our data showing that CAs and NPY, a sympathetic co-transmitter, modulate several
functions of macrophages (Regul. Pept. 2005;124:163-72), we would be interested in studying the
molecular mechanisms underlining these effects along with potential interaction between ARs and Y
receptor subtypes in sympathetic control of macrophage activities. ad 2. We have also shown Y
receptor subtype specific NPY modulation of inflammation and macrophage functions, as well as ageassociated alterations in Y1 receptor signaling (Exp. Gerontol. 2006; in press). As atherosclerosis is
widely prevalent in ageing population, and as monocyte-derived macrophages are involved in
pathogenesis of this disease, we would like to carry on further studies on putative contribution of an
altered NPY signaling to the atherogenesis by analysis the effects of NPY on the expression of various
immunologically relevant genes (e.g. pro-inflammatory cytokines) by macrophages.
Institution/University
Name of Institution/University
Faculty of Pharmacy
Number of Employees
200
Number of Researchers
110
Country
Serbia and Montenegro
Postal Adress
Vojvode Stepe 450
11152 Belgrade
Immunology and Immunohaematology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
MD PhD Dusan Popadic
Research Team Members
Team Member 0
MD PhD Dusan Popadic
Contact Person
Name
Dusan Popadic
Email
[email protected]
Function
Associate professor
Phone
+381 11 2657 258
Fax
+381 11 2657 258
Website
www.med.bg.ac.yu
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Neurology
Autoimmune/Inflammatory diseases; Multiple sclerosis; Experimental allergic encephalomyelitis;
Psoriasis; Cell culture; Flow-cytometry; Gene expression; Real-Time RT PCR
Partners/Interests
researcher
industrial partner
Immunology and Immunohaematology
Neurology
My partner should have similar research or commercial interest and background in manufacturing of
vitamin A and D and polyunsaturated fatty acids compounds or experiance in treatment of
inflammatory diseases with the available medicaments formulated on the analogs of vitamin A and D
or polyunsaturated fatty acids.
To test synthethic analogs of vitamin A and D and polyunsaturated fatty acids on lymphocyte and
keratinocyte biology in terms of potential ability of those substances to modulate natural course of
diseases such are Multiple sclerosis and Psoriasis vulgaris.
Institution/University
Name of Institution/University
Institute of Microbiology and Immunology
Number of Employees
45
Number of Researchers
25
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 1
11000 Belgrade
Immunology and Immunohaematology
Research Team
Research Team Name
Biological effect of dental materials
Individual Researcher
No
Research Team Leader Name
Assistant professor Sonja Pezelj-Ribaric
Research Team Members
Team Member 0
assistant Domagoj Maričić [[email protected]]
Team Member 1
assistant Vlatka Mikić [[email protected]]
Team Member 2
assistant Davor Kuiš [[email protected]]
Team Member 3
assistant Vladimir Ahel [[email protected]]
Team Member 4
assistant Silvio Ferreri [[email protected]]
Team Member 5
assistant Irena Glažar [[email protected]]
Team Member 6
assistant Hrvoje Gazdik [[email protected]]
Team Member 7
assistant Jelena Horvat [[email protected]]
Team Member 8
asisstant professor Renata Gržić [[email protected]]
Team Member 9
assistant professor Ivana Brekalo Pršo [[email protected]]
Contact Person
Name
Assistant professor Sonja Pezelj-Ribaric
Email
[email protected]
Function
Head of department
Phone
++38551633455
Fax
++38551345655
Website
medri.hr
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Clinical microbiology
Dentristry
Null-hypothesis is that there will be no difference between expected and obtained results during
biocompatibility, immunohistochemical, allergological, microbiological and epidemiological analyses.
The problem with composites, prosthodontic and restorative metals, drugs used in treatment of
various pathological changes of oral mucosa, as well as usage of various preparations which can
change their state of matter is that they can act as toxic or allergenic. Therefore the project wishes to
answer: in what way does the tissue respond to new dental materials in in vitro and in vivo
conditions. The assumption is that the raw materials used in production of different purpose dental
materials have already been tested during production stage, and have been declared as
biocompatible, but chemical properties of raw materials as well as newly-formed compounds can be
altered. This way, and during tissue interactions, biocompatibility can be decreased. Therefore it is
very important to run permanent tests on preparations regarding their degree of conversion and
duration of contact with living tissue. The assumption is that results will facilitate choice of dental
materials in clinical work. Lack of epidemiological data on condition of oral mucosa, especially in
population of Rijeka and specifically older population (retired persons) is imposed as one of chief
scientific and clinical problems. We can assume further that epidemiological analyses of oral health
condition and all the changes on oral mucosa will contribute to prevention and more efficient planning
of their clinical recovery. The occurrence of Candida species and subsequent pathological changes in
relation to prosthodontic treatment (especially removable prostheses) and treatment procedures of
oncologic patients are the focus of interest of many scientific investigations. Using microbiological
tests, and by isolation and identification of Candida species in relation to applied materials and
therapy, we can assume that better and faster cure will be enabled, thereby shortening the course of
disease. Proving the adherence ability, macrophage phagocitosis and microbicidal activity in relation
to the tested dental materials are of primary importance for tissue response. Determination of the
levels of pro-inflammatory cytokines using immunological tests can affect the choice of therapeutic
means.
Partners/Interests
researcher
Microbiology
Immunology and Immunohaematology
Pathology
Dentristry
Specialist in clinical microbiology Specialistin oral pathology
Biocompatibility tests using the analysis of tissue reaction after implantation of new materials for root
canal obturation: epoxy-resins, polyketons, silicones, composite resins, calcium-hydroxide and
materials for retrograde obturation: MTA, IRM, Super EBA applied into subcutaneous and bone tissue
of experimental animals. For experimental animals, Wistar rats will be used, of 180 -230 g weight (5
rats) which will be held at the vivarium of Department of pharmacology, Medical Faculty, University of
Rijeka. Rats will be kept in Plexiglas cages in groups of 6-8 on sawdust which is dusted and
autoclaved, and is changed regularly. Temperature of the filtered air, which is under positive
pressure, is 22 ± 2ºC, humidity 55- 60% and illumination of 300 Lux. Rats will be given water ad
libitum and fed using standardized palettes specially designed for laboratory rats. Rats will be
occasionally controlled for appearance of certain pathogens. Before initiation of the research, rats will
be anesthetized using halothane. Anesthesia will be implemented according to the instructions of the
existing immunology protocol (Care and Handling of Laboratory Animals). Afterwards we will implant
the materials into the subcutaneous tissue and animals will be sacrificed using CO2 as regulated by
the Law on Animal Well-being and international standards of the EU: EC Directive 86/609/EEC after 7
and 30 days, respectively. Histological evaluation will be performed by analyzing the number, type
and location of inflammatory cells. Reaction of bone tissue will be evaluated using silicone tubes
which will be implanted into the rats' tibiae. Rats will be sacrificed after 60 days after the
abovementioned protocol, after which we will perform histological and histomorphometrical analyses.
The expected duration of research is three years. b) Influence of the following materials to adherence
ability, phagocitosis and microbicidal activity of macrophages will be determined: epoxy-resins,
polyketons, silicones, composite resins, calcium-hydroxide, and materials for retrograde filling: MTA,
IRM, and Super EBA. Influence of dental materials on macrophages will be tested in in vivo
conditions. As a model, female mice will be used, aged 8-12 weeks. Laboratory mice will be put in
central vivarium of Medical Faculty, University in Rijeka, after standards set by European Union.
Institution/University
Name of Institution/University
Medical faculty
Number of Employees
350
Number of Researchers
100
Country
Croatia
Postal Adress
Brace Brancheta 20
51000 Rijeka
Immunology and Immunohaematology
Research Team
Research Team Name
Institute of Immunobiology and Human Genetics (IIBHG),
Faculty of Medicine, University "Ss Kiril and Metodij",
Skopje, Republic of Macedonia
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Mirko Spiroski
Research Team Members
Team Member 0
MD Jordan Petrov [[email protected]]
Team Member 1
Molecular biologist Olgica Sibinovska [[email protected]]
Team Member 2
MD Eli Spiroska [[email protected]]
Team Member 3
MD Slavica Hristomanova [[email protected]]
Team Member 4
Biotech Olivija Efinska-Mladenovska [[email protected]]
Team Member 5
MD Ana Strezova [[email protected]]
Team Member 6
MD, MSc Aleksandar Petlichkovski [[email protected]]
Team Member 7
MD, MSc Dejan Trajkov [[email protected]]
Research Team Projects
Project Name
Ambiguities Resolution of HLA Genotypes in Macedonian
Population
Project Leader
Prof. Dr. Mirko Spiroski [[email protected]]
Project Funding Agency
INTERNATIONAL CENTRE FOR GENETIC ENGINEERING
Project Budget
36000 €
Project Start Date
2004-01-01
Project End Date
2006-12-31
Project Partners
Institute of Immunobiology and Human Genetics (IIBHG), Faculty
of Medicine, University "Ss Kiril and Metodij", Skopje, Republic of
Macedonia; Macedonian Donor Registry and Bone Marrow
Transplantation Dimitar Efremov, M.D., Ph.D. Associate Professor of
Medicine and Group Leader Clinic for Haematology, Faculty of
Medicine, Vodnjanska 17, 1109 Skopje, Macedonia. present
address: CNR Campus "Adriano Buzzati-Traverso" Via E. Ramarini
32 I-00016 Monterotondo Scalo (Rome), Italy tel: +39-3337199691 fax: +39-06-90091260 E-mail: [email protected]
Project Summary
A total of 580 persons from the Republic of Macedonia divided in the four groups will be DNA-HLA
genotyped: 1. Donors (unrelated healthy persons) of the Macedonian Donor Registry (250); 2.
Patients for bone marrow transplantation and related potential donors (brothers, sisters, and parents)
(50); 3. Patients with rheumatoid arthritis, tuberculosis, cardiomyopaties and other diseases (150);
and 4. Ambiguity resolution of the genotypes (130). Genomic DNA will be isolated from peripheral
blood of individuals according to standard phenol/chlorophorm procedure. Class I HLA genes (A, B,
and C)will be typed by Reverse Line Strip Typing (RLS); Class II HLA gene (DRB!) will be typed by
Sequence Based Typing (SBT). Three different strategies will be used for ambiguities resolution: 1)
Comparison of the genotype with the genotypes of the family members; 2) Use of group specific
primers (GSAP); and 3) Use of sequence specific primers (SSP) for amplification of specific allels.
Data of the DNA-HLA typing will be stored in Excel files specified by the 13th International
Histocompatibility Working Group. Software for Population Genetic data Analysis (Arlequin, ver. 2000)
will be used for statistical analysis of allels, haplotypes, Hardy-Weinberg equilibrium, Pairwise linkage
disequilibrium by Slatkin, Ewens-Watterson tests of selective neutrality, Chakraborty's test of
selective neutrality and other indices. Project objectives are: 1. Creation of Macedonian Donor
Registry (MDR) and connection with the European and World Marrow Donor Registries. 2. HLA typing
of unrelated healthy persons using methods already established in our laboratory: RLS for Class I
HLA-A, -B, and -C typing, and SBT for Class II HLA-DRB1 typing. 3. HLA typing of patients for bone
marrow transplantation and related potential donors (brothers, sisters, and parents). 4. HLA typing of
samples from the patients with rheumatiod arthritis, tuberculosis, cardiomyopathies, and other
diseases associated with HLA allels. 5. Evaluation of allele and genotype ambiguities for Class I and
Class II typings and definition of strategy for their resolution. 6. Introduction and establishment of
Immunology and Immunohaematology
sequencing based methods for typing of Class I HLA -A, -B, and -C loci. 7. Introduction and
establishment of method that uses sequence specific primers (SSP) for resolving the ambiguous
results for Class II typing.
Project Website
www.immunology.edu.mk
Project Name
Molecular Analysis of Cytokine Gene Polymorphisms in
Republic of Macedonia
Project Leader
Prof. Dr. Mirko Spiroski [[email protected]]
Project Funding Agency
Ministry of Education and Sciences, Macedonia
Project Budget
15000 €
Project Start Date
2006-07-01
Project End Date
2009-06-30
Project Partners
Institute of Immunobiology and Human Genetics (IIBHG), Faculty
of Medicine, University "Ss Kiril and Metodij", Skopje, Republic of
Macedonia; Institute of Special Education and Rehabilitation,
Faculty of Philosophy, University "Ss. Kiril and Metodij", Skopje,
Republic of Macedonia; Dental Clinical Center, Department of Oral
Pathology and Periodontology, Faculty of Stomatology, University
"Ss. Kiril and Metodij", Skopje, Republic of Macedonia; Clinic for
Pediatrics, Clinical Center, Faculty of Medicine, University "Ss. Kiril
and Metodija, Skopje, Republic of Macedonia.
Project Summary
Cytokine gene polymorphism of gamma-interferon (IFNg); interleukin (IL) 1 alpha (IL-1a); IL-1 beta
(IL-1b); IL-1 receptor (IL-1R); IL-1R antagonist (IL-1RA); IL-2; IL-4; IL-4 receptor alpha (IL-4Ra);
IL-6; IL-10; IL-12B; TGF beta 1 (TGF-b1); and TNF alpha (TNF-a) will be determined in healthy
population leaving in the Republic of Macedonia (Macedonians, Albanians, Roma); patients with
pulmonary tuberculosis; patients with parodontopathia; patients with atopic dermatitis; patients with
reumatoid arthritis; patients with bronchial asthma; patients with chronic obstructive bronchitis; and
patients with dilated cardiomyopathy. Association of cytokine gene polymorphism with the above
diseases will be analyzed.
Project Website
www.immunology.edu.mk
Project Name
Blood Homocysteine Level and Prevalence of C677T Mutation
of Enzyme Methylentetrahydropholate Reductase (MTHFR)
as a Risk Factors for Blood Vessel Diseases.
Project Leader
Prof. Dr. Slobodanka Dzhekova-Stojkova [[email protected]]
Project Funding Agency
Ministry of Education and Scineces, Macedonia
Project Budget
10000 €
Project Start Date
2003-07-01
Project End Date
2006-06-30
Project Partners
Institute of Medical and Experimental Biochemistry, Faculty of
Medicine, University "Ss Kiril and Metodij", Skopje, Republic of
Macedonia; Institute of Immunobiology and Human Genetics
(IIBHG), Faculty of Medicine, University "Ss Kiril and Metodij",
Skopje, Republic of Macedonia; Institute of Transfuziology, Skopje,
Republic of Macedonia.
Project Summary
Homocysteine (Hcy) is natural amino acid that has attained greater importance in the clinical
investigations. Epidemiological studies performed among general population have suggested
association between the increased level of circulatory Hcy and premature coronary, cerebral and
peripheral atherosclerosis and venous thromboembolism. The aim of this investigation is introduction
and eventual modification of a method for Hcy determination as well as determination of the
reference values for the population in R. Macedonia. The intention is to determine the concentration
of Hcy in patients with coronary artery disease (CAD) and deep venous thrombosis (DVT); to examine
the prevalence of C677T mutation of enzyme methylentetrahydropholate reductase (MTHFR) and to
analyze the correlation of MTHFR C677T genotype with the Hcy values in serum of both healthy
population and patients with the concerned diseases. By accomplishing the outlined aims of the
investigation, for the first time in our country we expect to introduce a method for determination of
Hcy concentration in serum. We expect to obtain significantly higher values for Hcy in the groups of
Immunology and Immunohaematology
patients with CAD and DVT in comparison with the healthy population. By examining the gene of the
enzyme MTHFR, we assume to get information on the prevalence of its C677T mutation in healthy
population and in the examined diseases. In addition to this, we anticipate to have insight in the
correlation between the normal and mutated allele and Hcy level in the serum of both healthy
subjects and examined patients. Using combined vitamin therapy (B6, B12 and folate) reduction of
Hcy concentration could be provoked, thus its determination could be one component in preventing
vascular diseases.
Project Website
www.immunology.edu.mk/
Project Name
HLA-DNA Investigations in the Families with Autism in the
Republic of Macedonia
Project Leader
Assist. Prof. Dragoslav Kopachev [[email protected]]
Project Funding Agency
Ministry of Education and Science, Macedonia
Project Budget
10000 €
Project Start Date
2001-07-01
Project End Date
2004-06-30
Project Partners
Institute of Special Education and Rehabilitation, Faculty of
Philosophy, University "Ss. Kiril and Metodij", Skopje, Republic of
Macedonia; Institute of Immunobiology and Human Genetics
(IIBHG), Faculty of Medicine, University "Ss Kiril and Metodij",
Skopje, Republic of Macedonia
Project Summary
The frequencies of HLA-DNA alleles will be analyzed at the children with autism, their parents, and
their siblings in the Republic of Macedonia who are diagnosed with ICD-10 and DSM-IV criteria. The
research includes 31 autistic persons and 31 health persons. Families who have autistic children will
be registered in special forms and will be stored in data base. The frequencies of HLA-DNA A, B, C,
and D alleles will be determine with high resolutive molecular biologic techniques such as SSOP
(Sequence Specific Oligonucleotide Probes), RLS (Reverse Line Strip), and/or SBT (Sequencing-based
Typing). For establishing the differences in frequencies of HLA-DNA alleles between the health and
autistic children, also between parents and autistic children, and between these children and their
siblings will be made a statistic analysis. The results will be presented and published.
Project Website
http://def.fzf.ukim.edu.mk/
Project Name
Serum Immunoglobulins and Specific Food Allergens in the
Persons with Autism in the Republic of Macedonia
Project Leader
Prof. Ljupcho Ajdinski [[email protected]]
Project Funding Agency
Ministry of Education and Science, Macedonia
Project Budget
10000 €
Project Start Date
2000-07-01
Project End Date
2003-06-30
Project Partners
Institute of Special Education and Rehabilitation, Faculty of
Philosophy, University "Ss. Kiril and Metodij", Skopje, Republic of
Macedonia; Institute of Immunobiology and Human Genetics,
Faculty of Medicine, University "Ss Kiril and Metodij", Skopje,
Republic of Macedonia.
Project Summary
Autism is wide continuum of connected cognitive and neurobehavioral disorders, including three
features: impairments in socialization, impairments in verbal and nonverbal communication, and
restrictive and repetitive patterns of behavior. From 35 persons blood was taken, such as 22 their
brothers/sisters, 27 mothers, and 23 fathers in the period between the July 2000 to the June 2003
year. Ten milliliters of venous blood was drawn from each donor by the standard venipuncture in
vacutaners with EDTA (K3). At the time of blood drawing, none of autistic children were receiving any
medication or antipsychotic drug. Plasma samples were separated by centrifugation and stored at –
200C till the determination. Serum immunoglobulin classes and subclasses are determined
immunonephelometric by automated Dade-Behring Nephelometer Analyzer. Serum specific food
allergens are determined by Pharmacia UniCAP 100 in vitro automated solid phase
immunofluorescence. Plasma concentration of IgG4 subclass immunoglobulin is significantly higher in
persons with autism (p < 0.02). Statistically significant higher plasma concentration of IgG antibodies
against alfa-lactalbumin, beta-lactoglobulin and casein is found in autistic persons compared to their
Immunology and Immunohaematology
parents (p < 0.001). The concentration of IgG gliadin antibodies in autistic persons was found to be
higher compared to their mothers and fathers which is statistically significant (p < 0.04). Plasma
concentration of specific allergic IgA antibodies against beta-lactoglobulin in persons with autism is
significantly higher compared to brothers and sisters (p < 0.05). The level of casein IgA antibodies in
autistic group was significantly higher compared with those of their mothers and fathers (p < 0.01)
and brothers/sisters (p < 0.05). The level of casein and beta-lactoglobulin specific allergic IgE
antibodies in the serum of autistic patients was significantly higher compared with those of their
brothers/sisters and their parents (p < 0.04). The level of total IgE antibodies was statistically
significant higher in autistic persons compared to their mothers (p<0.04) and their brothers/sisters (p
< 0.02). The established immunological and allergological disturbances give more opportunities for
realization of immunodiagnostic, immunotherapy, and starting a diet without allergic products from
the food in Republic of Macedonia. Index terms: autism, specific allergic antibodies,
immunoglobullins, Republic of Macedonia.
Project Website
http://def.fzf.ukim.edu.mk/
Project Name
Determination of Lp(a), HDL, and LDL subclasses in children
population
Project Leader
Prof. Dr. Bojana Todorova [[email protected] ]
Project Funding Agency
Ministry of Education and Science, Macedonia
Project Budget
10000 €
Project Start Date
2000-07-01
Project End Date
2003-06-30
Project Partners
Institute of Medical and Experimental Biochemistry, Faculty of
Medicine, University "Ss Kiril and Metodij", Skopje, Republic of
Macedonia; Institute of Immunobiology and Human Genetics
(IIBHG), Faculty of Medicine, University "Ss Kiril and Metodij",
Skopje, Republic of Macedonia; Clinic for Childrens Disease, Clinical
Center-Skopje, Faculty of Medicine, University "Ss Kiril and
Metodij", Skopje, Republic of Macedonia.
Project Summary
Lipoprotein (a) [Lp(a)] is a plasma particle, similar in size and composition to the LDL, composed of
Apo(a) ¡Vprotein unique for Lp(a) and ApoB-100 linked with one or more disulfide bonds. High plasma
Lp(a) concentrations as well as low molecular weight Apo(a) isoforms are thought to be an
independent risk factor for atherosclerosis development. Low density lipoprotein (LDL) and high
density lipoprotein (HDL) are heterogeneous classes composed of several subclasses which differ in
density, size and composition, and this heterogeneity is thought to be genetically influenced. Small
LDL particles are thought to be more atherogenic than larger LDL subclasses, and their frequency
may depend on plasma lipid and apoprotein levels. The development of atherosclerosis is also afected
by specific pattern of plasma HDL subclasses. The aim of this project was to determine Apo(a)
isoforms and particle size distribution of LDL and HDL subclasses by gradient gel electrophoresis and
immunoblotting technique in both healthy children and children with diabetes mellitus and renal
diseases. In healthy children population in the Republic of Macedonia, the most frequently found were
HMW isoforms (>S4, S4, S4S3,>S4S4) associated with low plasma Lp(a) concentrations. Molecular
mass of the isoforms was in the range of 451-781kDa and the distribution of Lp(a) plasma
concentration (mean „b SD: 11.95 „b 5.97 mg/dL) was shifted towards low concentration in most of
the subjects (98%) having Lp(a) plasma concentration bellow the risk limit of 30 mg/dL. As expected,
there was a statistically significant inverse correlation between Apo(a) isoform size and Lp(a) plasma
level (r=-0.4257, p<0.001). Except statistically significant elevation in plasma Lp(a) concentration in
children with renal diseases, we found no other differences in determined parameters in diabetic and
children with renal diseases in comparison with healthy children. Analysis of LDL phenotype has
shown that in a big percent of healthy children (89%) bigger LDL1 and LDL2 subclasses were
dominant, that is, phenotype A, whereas 11% of the children belonging to phenotype B were
characterized by the presence of small, atherogenic LDL3 and LDL4 subclasses. The distribution of
HDL subclasses showed domination of bigger HDL2b and HDL2a subclasses in 94,5%, whereas only in
5,5% of the children small HDL3a and HDL3b subclasses were dominant. These results have revealed
an antiatherogenic LDL and HDL subclass profile in healthy children in R. Macedonia. Although there
were no differences in the plasma lipid profile in children with insulin dependent diabetes mellitus
compared with healthy children, the frequency of phenotype B was increased (88,5%), and the mean
LDL diameter was smaller (p<0,0001). These changes in LDL subclasses distribution were especially
evident in children with renal diseases where even 95% belonged to atherogenic phenotype B. HDL
subclasses distribution in both patient groups was also shifted towards smaller, atherogenic HDL
subclasses. These findings confirm the fact that in children with diabetes mellitus and renal diseases
Immunology and Immunohaematology
the risk for atherosclerosis development is increased. Determination of LDL and HDL subclasses
distribution and Apo(a) isoforms as well as plasma Lp(a) levels in healthy children population and in
patients, may help in preventing and reduceing the risk for atherosclerosis development.
Project Website
Project Name
Plasma LDL and HDL lipoproteins and their phenotypes
Project Leader
Assist. prof. Sonja Alabakovska [[email protected] ]
Project Funding Agency
Ministry for Education and Science, Macedonia
Project Budget
15000 €
Project Start Date
2006-07-01
Project End Date
2009-06-30
Project Partners
Institute of Medical and Experimental Biochemistry, Faculty of
Medicine, University "Ss Kiril and Metodij", Skopje, Republic of
Macedonia; Institute of Immunobiology and Human Genetics
(IIBHG), Faculty of Medicine, University "Ss Kiril and Metodij",
Skopje, Republic of Macedonia; Clinic for Childrens Disease, Clinical
Center-Skopje, Faculty of Medicine, University "Ss Kiril and
Metodij", Skopje, Republic of Macedonia.
Project Summary
Low density lipoprotein particles (LDL) and high density lipoprotein particles (HDL) are heterogeneous
fractions composed of several subclasses different in density, size and chemical composition. The
lipoprotein profile, characterized by the domination of LDL-1 and LDL-2 subclasses is called LDL
phenotype A and the lipoprotein profile characterized by the domination of LDL-3 and LDL-4
subclasses is called phenotype B. Small LDL particles are thought to be more atherogenic than larger
LDL subclasses and increase of the concentration of sLDL particles (phenotype B) is associated with a
3 times greater risk of heart attack. The development of atherosclerosiss is also afeceted by a specific
pattern of plasma HDL particles subclasses. Small HDL3a, HDL3b and HDL3c subclasses which accept
cholesterol from peripherial cells less efficient, increases the risk of development atherosclerosis. The
data from several studies, as well as our own results proof the role of LDL and HDL subclasses in the
development of atherosclerosis. For further investigartion of the role of LDL and HDl in the
development of CAD we would like to anlyse the association between progression of the coronary
artery lesion (rate of arterial stenosis) and the concnetration of small lipoprotein subclasses.
Althdough it is considered that lipoprotein distribution is geneticaly determined, rescently, there are
more evidence showing the importance of the non-genetic factors such as: high concentration of
plasma triglycerides, low concnetration of HDL cholesterol, diet, age and the hormonal status of the
females. It is well known fact that the risk for atherosclerosis development is higher in males than in
females. Our results as well as literature data show that LDL lipoprotein profile type B is more
frequently found in males than in females. But, rescent investigations sugesst that this picture is
changing in the period of postmenopause in females when plasma concentration of triglyceride and
LDL choletesterol are increased, HDL cholesterol is decresed and lipoprotein subclass distribution
changes towards smaller atherogenic subclasses. Therefore, lately the attention of the investigators is
focused on the effect of hormonal replacement therapy with estrogens in postmenopausal women on
the lipid profile and especially on LDL and HDL subclass distribution. Therefore, second challenge in
this project would be following the effect of the hormeone replacement therapy with estorgens which
may present an excellent model for evaluation of the antiatherogenic influence of the estrogens on
the lipid methabolism. The aim of this project is to determine particle size distribution of LDL and HDL
subclasses phenotype by gradient gel electrophoresis in patients with coronary artery disease and to
follow up the relationship between lipoprotein subclasses and the rate of coronary artery stenosis
determined by selective coronarygraphy as well as the carotid artery intima-media thickness
determined by carotid ultrasonography . Also, phenotypization of the LDL and HDL subclass profile
will be determined in the postmenopausal women before and after the hormpne replacement therapy,
folowing the effect of the therapy on the lipoprotein profile. In both groups of patients correlation will
be performed between lipoprotein phenotype and other lipid and apoprotein parameters determined.
We expect that the results from this study will point the necesserity of the LDL and HDL subclass
distribution determination in each patient. It will help in the diagnose of the subjects with increased
risk of appearance and progression of the atherosclerotic changes of the blood vesels as well as for
determining the respective hipolipidemic therapy and hormone replacement therapy as an prevention.
Project Website
Immunology and Immunohaematology
Contact Person
Name
Prof. Dr. Mirko Spiroski
Email
[email protected]
Function
Director, professor of immunology and human genetics
Phone
389 2 3110556
Fax
389 2 3110558
Website
immunology.edu.mk/
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Genetics
Public health services
- Immunodiagnostcs: Acute phase proteins, Ig classes, Ig subclasses, total and specific IgE
(allergology), autoantibodies, cytokines, flow cytometry. - Immunogenetics: HLA-DNA genotyping
(HLA-A, -B, -C, -DRB1, -DQ), cytokine gen polymorphisms, killer cell immunoglobulin-like receptors
(KIRs) polymorphism. - Human Genetics: cardiovascular disease (CVD) mutations (FV G1691A
(Leiden), FV H1299R (R2), Prothrombin G20210A, Factor XIII V34L, ¥â-Fibrinogen -455 G-A, PAI-1
4G/5G, GPIIIa L33P (HPA-1), MTHFR C677T, MTHFR A1298C, ACE I/D, Apo B R3500Q, Apo
E2/E3/E4), ALAD, ©¬-Globin, Haemochromatosis, FMF, Gaucher Disease, Sugar Intolerance.
Partners/Interests
researcher
Immunology and Immunohaematology
Genetics
Public health services
Immunology, molecular biology, immunogenetics, human genetics
Institution/University
Name of Institution/University
Institute of Immunobiology and Human Genetics (IIBHG)
Number of Employees
10
Number of Researchers
7
Country
Macedonia
Postal Adress
50 Divizija No 6, PO Box 60 6
1109 Skopje
Immunology and Immunohaematology
Research Team
Research Team Name
Interferons
Individual Researcher
No
Research Team Leader Name
Mr., MD Ales Goropevšek
Research Team Members
Team Member 0
Mr. MD, PhD Ivan Krajnc []
Team Member 1
Mrs. PhD Avrelija Cencic []
Contact Person
Name
Mr., MD Ales Goropevšek
Email
[email protected]
Function
researcher
Phone
+ 386 2 321 10 00
Fax
+386 2 331 23 93
Website
www.sb-mb.si
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Interferons
Partners/Interests
researcher
industrial partner
Suitable
Immunology and Immunohaematology
1. INTERFERONS AND CYTOKINES IN CHRONIC INFLAMMATORY DISEASES Almost all current
therapeutic concepts in chronic inflammatory diseases are based on the systemic suppression of
immune functions and are not curative. Identification of cytokines TNF and IFN-α as major factors in
the pathogenesis of diseases such as rheumatoid arthritis and systemic lupus erythematosus (SLE)
represent a substantial improvement in understanding of autoimmune diseases. However, knowledge
of the molecular mechanisms underlying and regulating the production of type I interferons is still
lacking . Professional type I interferon-producing cells also known as plasmacytoid dendritic cell
precursors (pDCs) represent unique subpopulation of dendritic cells. Together with other DCs they
play a master role in the control of immunity and in host-pathogen interactions. The study of DCs and
their subpopulations was limited by the absence of specific markers and their low frequency in human
and mouse tissues and blood . Because pDCs undergo rapid spontaneous apoptosis in culture in vitro
studies are also very limited. We succeeded in establishment of a first novel pDC cell line, which will
give us oportunity to study pDCs in vitro. Phospho-proteomic immune analysis by flow cytometry will
give us biochemical access to rare cell subsets such as those from clinically derived samples or
populations that comprise too few in numbers for conventional biochemical analysis. Studies of
multiple activated signaling pathways (in complex populations of cells) at the single-cell level will be
done using various (TLR) agonists and kinase inhibitors. This will give us insight into mechanisms of
cytokine (IFN-alpha) induction, action and its downstream mediators. Single-cell signal network
analysis can also be used to stratify patients and may be useful for understanding mechanisms of
disease progression, treatment resistance, and development of diagnostic indicators. Understanding
(p)DC biology holds future promise for developing cures for autoimmune diseases, infectious
diseases, and cancer. Since the mid-1990s, an immense number of kinase inhibitors has been
characterised in vitro, and to date several compounds have been advanced into clinical trials. Small
molecular, orally active anti-cytokine agents, which target specific pathways of proinflammatory
cytokines, would offer an attractive alternative also to anti-cytokine biologicals.
Institution/University
Name of Institution/University
General Hospital Maribor
Number of Employees
2400
Number of Researchers
33
Country
Slovenia
Postal Adress
Ljubljanska 5
2000 Maribor
Immunology and Immunohaematology
Research Team
Research Team Name
Laboratory for Imunnoneuroendocrine network research
Medical faculty Belgrade & Medical faculty Foca University of
East Sarajevo
Individual Researcher
No
Research Team Leader Name
M.D., Ph.D., physiologist Vojislav Pantic
Research Team Members
Team Member 0
assistant Dejan Bokonjic []
Team Member 1
assist professor Snezana Medenica []
Team Member 2
assist professor Milan Kulic []
Team Member 3
professor, hystolog Senka Pantic []
Team Member 4
professor, physiolog Vojislav Pantic []
Team Member 5
assistant Milica Kunarac []
Team Member 6
assistant Starovic Suncica []
Team Member 7
assist professor Sinisa Ristic []
Research Team Projects
Project Name
Immunotoxicology of tobacco smoking
Project Leader
assistant professor Sinisa Ristic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2007-01-01
Project End Date
2010-01-01
Project Partners
Project Summary
role of neuroendocrine system in immunotoxicology of tobacco smoking
Project Website
role of vegetative nervous system in immunoregulation
Project Name
full professor vojislav pantic [[email protected]]
Project Leader
Project Funding Agency
€
Project Budget
0001-01-01
Project Start Date
0001-01-01
Project End Date
Project Partners
Project Summary
Project Website
Contact Person
Name
M.D., Ph.D., physiologist, radiologist sinisa ristic
Email
[email protected]
Function
assistant profesor
Phone
00 387 65 44 76 88
Fax
00 387 58 210 007
Website
www.medfak.srbinje.net
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Physiology
Toxicology
Immunology and Immunohaematology
immunoneuroendocrine network research - role of vegetative nervous system and various hormones
in regulation of immune response, immunotoxicology of tobacco smoking
Partners/Interests
researcher
Toxicology
Physiology
Immunology and Immunohaematology
basic researcher in biomedicina
role of indirect mechanism (neuroendocrine system and their signal molecules) in immunotoxicology
of tobacco smoking animal model, cell culture
Institution/University
Name of Institution/University
Medical faculty Foca
Number of Employees
150
Number of Researchers
20
Country
Bosnia and Herzegovina
Postal Adress
Solunskih dobrovoljaca 1
73300 Foca
Immunology and Immunohaematology
Research Team
Research Team Name
Research group in Osteoimmunology/Hematology;
Department of Physiology and Immunology/University of
Zagreb School of Medicine/Zagreb/Croatia
Individual Researcher
Yes
Research Team Leader Name
MD, PhD Danka Grcevic
Research Team Members
Team Member 0
PhD student Vedran Velagic [[email protected]]
Team Member 1
MD, PhD Zrinka Jajic [[email protected]]
Team Member 2
PhD student Anita Lukic [[email protected]]
Team Member 3
MD, PhD Danka Grcevic [[email protected]]
Research Team Projects
Project Name
T- and B-lymphocytes in the regulation of bone metabolism
Project Leader
MD, PhD Danka Grcevic [[email protected]]
Project Funding Agency
Croatian Ministry of Science, Education
Project Budget
50000 €
Project Start Date
2002-07-01
Project End Date
2006-06-31
Project Partners
Croatia/University of Zagreb School of Medicine/Department of
Physiology and Immunology
Project Summary
In addition to their physical proximity, immune and bone systems are also functionally related. T- and
B-lymphocytes produce cytokines and growth factors that may regulate bone cells and bone
remodeling, directly or indirectly. Members of the tumor necrosis factor-related family of molecules
are specifically important: receptor activator of nuclear factor kappa-B (RANK), RANK-ligand (RANKL)
and osteoprotegerin (OPG). Those molecules are essential regulators of the differentiation and
function of both bone and immune cells. RANKL stimulates bone resorption after binding to activation
receptor RANK, whereas OPG acts as decoy receptor, which blocks RANKL/RANK interaction. The aim
of the proposed research is to investigate the role of T- and B-lymphocytes in bone metabolism. Since
lymphocytes may secrete both osteoresorptive and osteoprotective cytokines, we hypothesized that
changes in bone marrow lymphocyte populations would disturb the cytokine balance, and, therefore,
affect the differentiation and function of bone cells. The investigation will be performed through
several phases in vivo and in vitro, on the mouse model: 1. Bone cell differentiation will be followed
after depletion of different lymphocyte populations with monoclonal antibodies, 2. Effect of activated
lymphocytes will be studied in vivo on the inflammatory model (after administration of bacterial
lipopolysaccharide) or after immunization with allogeneic cells, and in vitro after addition of activated
lymphocytes in osteoblast or osteoclast bone marrow cell cultures, 3. Immunosuppressants
cyclosporin A and glucocorticoids will be used in vivo and in vitro to investigate the effect of
immunosuppression on the differentiation and function of bone cells. We expect that depletion,
suppression or activation of lymphocyte populations will affect the bone remodeling, specifically
through changes in RANKL/RANK/OPG system. Those effects will be assessed by the analysis of
osteoclast and osteoblast differentiation, gene expression for regulatory molecules and phenotypic
markers of bone marrow cells. The results of proposed investigation would add to the understanding
of a number of pathological states that develop in bone microenvironment and affect bone
metabolism, but not originated from bone cells. Some examples are posttransplantation osteoporosis,
rheumatoid arthritis, osteomyelitis, periodontitis, certain immunodeficient disorders, chronic viral
infections and hematopoietic malignancies.
Project Website
www.mzos.hr
Project Name
Molecular Interactions between Bone and Bone Marrow
Project Leader
MD, PhD Ana Marusic [[email protected]]
Project Funding Agency
Croatian Ministry of Science, Education
Project Budget
75000 €
Project Start Date
2002-07-01
Project End Date
2006-06-31
Immunology and Immunohaematology
Project Partners
Croatia/Zagreb University School of Medicine/Department of
Anatomy
Project Summary
The project is dealing with the functional interactions between bone and bone marrow using different
in vivo and in vitro models.
Project Website
www.mzos.hr
Project Name
Common progenitor of B-lymphocytes and osteoclasts
Project Leader
MD, PhD Vedran Katavic [[email protected]]
Project Funding Agency
Croatian Ministry of Science, Education
Project Budget
30000 €
Project Start Date
2002-07-01
Project End Date
2006-06-31
Project Partners
Croatia/University of Zagreb School of Medicine/Department of
Anatomy
Project Summary
The project is dealing with the common hematopoietic progenitor cells of B-lymphocytes and
osteoclasts, particularly their characterization and regulatory mechanisms.
Project Website
www.mzos.hr
Project Name
Interactions Between Bone and the Immune System: A role
for Fas-Mediated Apoptosis
Project Leader
MD, PhD Ana Marusic [[email protected]]
Project Funding Agency
The Wellcome Trust Collaborative Research Ini
Project Budget
50000 €
Project Start Date
2004-05-01
Project End Date
2006-04-30
Project Partners
Ana Marusic/Croatia/University of Zagreb School of
Medicine/Department of Anatomy; Collaboration with Prof. Peter
Croucher, Sheffield University, UK
Project Summary
The project is dealing with the importance of the Fas ligand - Fas mediated apoptosis in the regulation
of bone cell differentiation and survival.
Project Website
www.wellcome.ac.uk
Contact Person
Name
MD, PhD Danka Grčević
Email
[email protected]
Function
Assistant Profesor
Phone
385 1 4566 944
Fax
385 1 4590 222
Website
mef.hr
Research Team Objectives
Main Fields
Immunology and Immunohaematology
Biology
Osteoimunology, Bone biology, Immunohematology, Oncology
Partners/Interests
researcher
Immunology and Immunohaematology
Biology
Research experience in the field of Osteoimmunology/Immunohematology/Oncology
We propose a study within the novel field of osteoimmunology, with a specific interest in the
functional interactions between immune and bone systems. The main hypothesis is that the
disturbances, which mainly affect the immune system, cause the changes in the production of
cytokines and growth factors by affected immune cells, with the consequence in the disturbed
Immunology and Immunohaematology
differentiation and functions of bone cells and bone metabolism. We expect that the disturbances
originating mainly in lymphocytes will cause numerous changes in bone cell cultures in vitro an in the
microenvironment of diseases in vivo. This hypothesis will be tested on several models –
experimental mice that will enable us to use different in vivo models, cell lines that are suitable to
study the contribution of certain cell populations and cytokines in carefully controlled and optimal in
vitro conditions, and human samples to finally confirm the important role of the cytokine
microenvironment in the pathogenesis of certain human diseases.
Institution/University
Name of Institution/University
University of Zagreb School of Medicine
Number of Employees
500
Number of Researchers
250
Country
Croatia
Postal Adress
Šalata 3
10000 Zagreb
Information Technology
Research Team
Research Team Name
Image Processing Group
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Sven Loncaric
Contact Person
Name
Prof.dr. Sven Loncaric
Email
[email protected]
Function
Associate Professor of Electrical and Computer Engineering
Phone
+385-1-6129-891
Fax
+385-1-6129-652
Website
http://ipg.zesoi.fer.hr/loncaric
Research Team Objectives
Main Fields
Information Technology, Statistics, Documentation
Image Processing Group has expertise in developing methodology and computer programs for
processing and quantitative analysis of two-dimensional and three-dimensional images and image
sequences (video) obtained by various medical imaging techniques. Such methods can be applied to
any medical image in order to enhance images for human viewing, or for analysis of anatomical or
functional images. Applications are both in diagnostics and in interventions in medicine.
Partners/Interests
researcher
industrial partner
Information Technology, Statistics, Documentation
We seek a medical partner who requires research and development of new methods for analysis of
medical data, which can be in the form of signals, images, or videos.
We seek medical partners requiring our expertise in development of computer-based medical image
analysis methods.
Institution/University
Name of Institution/University
University of Zagreb, Faculty of Electrical Engineering
Number of Employees
250
Number of Researchers
200
Country
Croatia
Postal Adress
Unska 3
10000 Zagreb
Information Technology
Research Team
Research Team Name
Informatics
Individual Researcher
No
Research Team Leader Name
Mr., PhD Dejan Dinevski
Contact Person
Name
Mr., PhD Dejan Dinevski
Email
[email protected]
Function
Assistant Professor of Informatics
Phone
+ 386 2 234 56 01
Fax
+ 386 2 234 56 00
Website
http://www.mf.uni-mb.si/ang/main.htm
Research Team Objectives
Main Fields
Information Technology, Statistics, Documentation
Informatics
Partners/Interests
researcher
industrial partner
Suitable
Information Technology, Statistics, Documentation
1. TECHNOLOGY ENHANCED LEARNING PROCESSES (E-LEARNING AND BEYOND) IN MEDICINE The
quality of learning processes in medicine has an immense potential for improvement trough
implementation of “information and communication technologies” (as one of the priorities of FP7) in
learning and teaching. The learning processes to be considered in this scope are: learning, teaching,
administration, collaboration, construction of knowledge and networking of education institutions in
medicine. E-learning programme of the European commission has presented clear goals in the
development of all the mentioned processes, but their implementation in medicine would require
substantial research actions due to several specific features of the area. Three major research
subjects in this scope would be: 1. Implementation of E-learning standards/recommendations in
medicine 2. Quality of the e-learning resources in medicine 3. Effective implementation of Information
and communication technologies (multimedia, interactivity etc.) into medicine education
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Information Technology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
prof. dr. Uroš Stanič, univ.dipl.ing.el.
Contact Person
Name
prof. dr. Uroš Stanič, univ.dipl.ing.el.
Email
[email protected]
Function
Phone
+386 4 2569 162 / +386 40 207 103
Fax
+386 4 25 69 117
Website
http://www.klinika-golnik.si/
Research Team Objectives
Main Fields
Information Technology, Statistics, Documentation
Cybernetics and systems
Biomedical technology
Telemedicine
Partners/Interests
Telemedicine
Hospital Golnik has strong interest in cooperation in research, development and transfer of good
practice in the field of Telemedicine. The application is needed for 24 hours continuous management
of pulmonary and allergic patients population in Slovenia with possible exstension of network with
neighbouring countries (Austria,Italy, Hungary, Croatia and Western Balkan") and any other
interested EU member state and INCO countries."
Institution/University
Name of Institution/University
University Clinic for Respiratory and Allergic Diseases Golnik
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Golnik 36
4204 Golnik
Internal Medicine
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
MD, PhD Igor Aurer
Research Team Projects
Project Name
Diagnostic and treatment of lymphoma
Project Leader
Prof. Igor Aurer [[email protected]]
Project Funding Agency
Croatian Ministry of Science
Project Budget
75000 €
Project Start Date
2002-09-01
Project End Date
2007-09-01
Project Partners
Project Summary
Lymphoid neoplasms are one of 10 most frequent human tumors and are the only ones of those
whose incidence is growing all over the world. Despite treatment a significant number of patients die
from their disease or its complications. Because of this are improvements in diagnostic and treatment
of lymphoid neoplasms of major scientific and medical importance. The goal of this project is to
improve our knowledge on the usefulness of certain diagnostic and therapeutic approaches as well as
biologic characteristics of their disease in patients with lymphoid neoplasms. Using a comprehensive
team approach we intend to find out: -the prognostic significance of histochemical determination of
proliferative indices; -the usefulness of cytology for the diagnosis and classification of lymphoma in
comparison with the present golden standard, histology; -the usefulness of US in determining the
involvement of cervical and axillary lymph nodes in comparison with the present golden standard,
palpation; -the usefulness of positron emission tomography in determining treatment outcome in
comparison with the present golden standards, CT scanning and palpation; -the usefulness of
monitoring of typical molecular changes in the diagnosis and follow-up of lymphoma; -the usefulness
of monitoring of markers of coagulation and anticoagulation system activation for identification of
patients with high risk of thrombosis and prevention possibilities. In collaboration with international
cooperative groups, specially the EORTC Lymphoma Group, we plan to examine new chemo- and
radiotherapeutic treatment approaches for lymphoid neoplasms. The results of this project can
improve our understanding of the biology of these diseases, the rational use of diagnostic procedures
and treatment outcome in patients with lymphoid neoplasms.
Project Website
Contact Person
Name
MD, PhD Igor Aurer
Email
[email protected]
Function
Associate Professor of Medicine, Consultatnt Hematologist
Phone
385-1-2388-675
Fax
385-1-2421-892
Website
mef.hr
Research Team Objectives
Main Fields
Internal Medicine
Clinical studies in lymphoma. Biology of lymphomas.
Partners/Interests
researcher
Biology
Pathology
Internal Medicine
Abilities to perform similar studies
Clinical and basic studies in lymphoma
Institution/University
Name of Institution/University
Medical School, University of Zagreb
Number of Employees
n.a.
Internal Medicine
Number of Researchers
n.a.
Country
Croatia
Postal Adress
Salata 3
10000 Zagreb
Internal Medicine
Research Team
Research Team Name
Academy of Sciences and Arts of the Republic of
Srpska/department of cardiovascular pathology
Individual Researcher
No
Research Team Leader Name
Associate Professor Dusko Vulic
Research Team Members
Team Member 0
Professor Nikola Kocev [[email protected]]
Team Member 1
Professor Marija Burgic [[email protected]]
Team Member 2
Professor Jelena Marinkovic [[email protected]]
Team Member 3
Assist.Prof Sinisa Ristic [[email protected]]
Team Member 4
Professor Aleksandar Lazarevic [[email protected]]
Team Member 5
Ph.D. Dusko Vulic [[email protected]]
Team Member 6
Academician Drenka Secerov [[email protected]]
Team Member 7
Assis.Prof Snjezana Medenica [[email protected]]
Research Team Projects
Project Name
Education of Medical Doctors and Population in
Implementation of Measures for Cardiovascular Diseases
Prevention in Republic of Srpska
Project Leader
Ph.D. Dusko Vulic [[email protected]]
Project Funding Agency
Health Care Found republic of Srpska
Project Budget
25000 €
Project Start Date
2001-10-20
Project End Date
2002-10-20
Project Partners
Bosnia and Herzegovina/Foundation of health and heart Bosnia and
Herzegovina/University of Banja Luka,School of Medicine Banja
Luka Bosnia and Herzegovina/University of E.Sarajevo,School of
Medicine Foca
Project Summary
Within the realization of the Program for Prevention of Cardiovascular Diseases in the Republika
Srpska,Bosnia and Herzegovina the need for establishing a permanent education of medical doctors
and population in implementation of measures for cardiovascular diseases prevention was
emphasized in the Guidelines and Recommendations of the National Committee. Given the so-far
experience in cooperation with the European Heart Network, European Heart House, World Heart
Federation (WHF), RS Ministry of Health and Social Welfare, in organization of the program for
education of doctors and population a project has been prepared with the aim of providing education
to a group of doctors in primary health care who , as the trainers, perform education of the
population on the local level. This form of education conducted in the local communities by means of
public discussion on health, school classes dedicated to healthy way of life. Education programs for
doctors organized through educational centers (Banja Luka, Prijedor, Doboj, Bijeljina, Srbinje,
Trebinje) from where the activities of the trainers in the local communities coordinated.
Project Website
Project Name
Republic of Srpska Coronary Prevention Study
Project Leader
Ph.D. Dusko Vulic [[email protected]]
Project Funding Agency
Foundation of health and heart
Project Budget
10000 €
Project Start Date
2003-06-20
Project End Date
2003-12-20
Project Partners
Bosnia and Herzegovina/Foundation of health and heart Bosnia and
Herzegovina/Health Care Centres
Project Summary
our study demonstrated high prevalence of modificable risk factors in coronary heart disease.After
Internal Medicine
implementation guidelines we changed therapeutic aproach for treatment patient with CHD.It good
potential for secundary prevention our population in future.:In our study we analyse 125 patient at
2001/2002 , aged between 40 and 71, 20.8% women and 79.2% men. Foreteen percent of patients
smoked cigaretes, 17.6% were overweight (BMI „d 30kg/m2), 63.2% had raised blood pressure
(systolic BP„d 140 and/or diastolic BP „d 90mmHg), 52,8% had raised total plasma cholesterol(total
cholesterol „d 5,5mmol/l) and 27.2% were diabetic.Reported medication at interview
was:antiplatelets drugs 92%,beta-blockers 62,4%,ACE inhibitors 62,4%,lipid-lowering drugs
41,6%,calcium antagonist 16,8%,nitrates 59,4% and antidiabetcs 16,8% two years later after
imlementation New Joint European Guidelines. We compared with healthy group and analised
population atributive risk(PAR) and we got next results:smoking PAR 32,67%,OR 3,09 CI(1.825.24),psychosocial factor PAR 45.83%,OR 4.84,CI(2.77-8.46),hypertension PAR 10,67%,OR
1.43,CI(0.86-2.84),total cholestaerol PAR 8.19%,OR 1.37,CI(0.83-2,26),diabetes 5,2%,OR
1.57,CI(0.86-2.84) and physical activity 22,72%,OR 0.57,CI(0,29-1.11).
Project Website
Contact Person
Name
M.D., Ph.D. Dusko Vulic
Email
[email protected]
Function
assistant professor,
Phone
Fax
Website
Research Team Objectives
Main Fields
Internal Medicine
Anatomy
Public health services
Cardiology,Anatomy,Physiology,Psychology,Statistics
Partners/Interests
researcher
Internal Medicine
Psychiatry
Epidemiology
making network between similar research group
Project:Postraumatic predictors cardiovascular disease at the younge people Cardiovascular disease
(CVD), especially coronary heart disease (CHD) and cerebrovascular disease is one of the most
frequent causes of death in Danube Basin countries and in Russia, as it was published in the last
statistic WHO year-book. After the decrease of CVD mortality rate in 1980-1990 on the territory of ex
Yugoslavia, there was a new trend of increase in Bosnia and Herzegovina, Serbia and Montenegro and
Macedonia. Information show that mortality rate in postwar period was 56% in Republic of SrpskaB&H, 57% in Serbia and Montenegro in relation to all other causes of death. The research on
coronary heart disease risk factors shows high presence of modifiable risk factors (hypertension,
diabetes, hyperlipidaemia and smoking). Taking war into consideration, as well as forced life
conditions in Bosnia and Herzegovina in 1992-1996, young population was especially endangered. As
this population was continuously exposed to stress, inadequate and irregular nutrition, it is normal to
expect a new increase in CVD morbidity and mortality.The hypothesis saying that atherosclerosis
starts in childhood is well known. That's why the Yugoslavian study of precursors of atherosclerosis in
school children was started in 1997. The study involved 5429 children (2675 boys and 2754 girls),
aged 10, in 12 towns in Serbia and 1 town in Republic of Srpska-B&H. The study followed: body
weight, height, waist to hip ratio, skin crease on the arm and scapula, blood pressure, sedimentation
rate, cholesterol, triglycerides, HDL, LDL, blood glucose, hemoglobin, fibrinogen, leucocytes. The
groups are followed for 5 years, with treatment of discovered risk factors. First medical checkups
showed significant presence of risk factors in children, as well as that female children have higher
values of LDL, triglycerides, Apo-B than male children. Previous analyses of risk factors in coronary
heart disease patients showed high incidence of modifiable risk factors, especially smoking,
hypertension, diabetes and hyperlipidaemia (ROSCOPS I 2001). Considering forced life conditions,
psychosocial factors and lifestyle were major factors for high incidence of these risk factors. Psyche
burden induce events that manifest as changes in behavior and habits, and in that way they are in
close relation to physical events. Psychosocial factors must be observed comprehensively, taking into
consideration continuous stress, sudden stress, bad social situation and unemployment that lead to
expressive anxiety, depression and fear. All cited states, which are present in families on this
territory, contributed to bad habits like smoking, alcohol abuse, and unhealthy nutrition. All of these
Internal Medicine
created conditions for faster development of cardiovascular risk factors in young people. That
situation caught many people unprepared to resist or to adjust to those tumultuous times. The study
was done on the territory of Novi Sad – Serbia and it involved more that 1000 patients with
myocardial infarction, 25-64 years old, and it showed that these patients were exposed to stress 4
times more than the control group. Indicators of trends and determinants of CVD development on the
territory of Novi Sad show that there was a great increase of myocardial infarction, starting with
1991, which can be related to stress events that caught us. That's why it is necessary to make
organized studies of young people with taking measures for prevention and acting on risk factors. By
making a uniform system for surveillance of posttraumatic stress disorders and detection of risk
factors it would be possible to act at the right time for prevention of consequences and development
of CVD and other diseases. That is the only way that could stop the increase of morbidity and
mortality of these diseases on the territory of Republic of Srpska. In order to start the system of
continuous surveillance of the most endangered population it is necessary to make a network which
would include health institutions, schools and faculties, which would organize screening of risk factors
with organized work on measures for prevention in schools, faculties and through media. The
research would include a sample of 5000 young people in the age of 15-50 that were exposed to
forced life conditions during war and in postwar period on the territory of Republic of Srpska. This
sample would be followed in 12 centers in Republic of Srpska. The research would be conducted
during medical checkups of high school children and students in the time of admission to the first
year of study, and the following parameters would be checked: body weight, height, body mass
index, waist to hip ratio, blood pressure, EKG, cholesterol, triglycerides, HDL, LDL, blood glucose and
PTSP parameters. Values of selected predictors of atherosclerosis would be measured in risk groups.
After measurement, data would be recorded to the Project website, and processed afterwards. After
the research was conducted and risk groups were defined, preventive measures would be taken
through seminars, tribunes and working with risk groups. Selected parameters would be followed in
risk groups. For realization of all cited activities it is necessary to develop a network of surveillance
centers, to educate personnel, to get all necessary equipment and disposable supplies for surveillance
of defined parameters and processing of data by uniform methodology
Institution/University
Name of Institution/University
Banjaluka, university
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Bosnia and Herzegovina
Postal Adress
11
71000 B Luka
Internal Medicine
Research Team
Research Team Name
Department of Cardiology, Internal Medicine, University
Hospital Dubrava
Individual Researcher
No
Research Team Leader Name
Professor Mijo Bergovec
Research Team Members
Team Member 0
MD, MSc Jozica Sikic Vagic [[email protected]]
Team Member 1
MD, MSc Boris Starcevic [[email protected]]
Team Member 2
MD Hrvoje Vrazic [[email protected]]
Team Member 3
MD, MSc Miroslav Raguz [[email protected]]
Research Team Projects
Project Name
Inflammatory markers in acute coronary sydrome
Project Leader
professor Mijo Bergovec [[email protected]]
Project Funding Agency
Ministry of Science, Education and Sports
Project Budget
20000 €
Project Start Date
2004-02-19
Project End Date
2004-11-03
Project Partners
Croatia / School of public health "Andrija Stampar"
Project Summary
During the last decade, more attention is put towards inflammatory factors as on of the components
of the patogenesis of acute coronary syndrome. Some studies have shown that the serum levels of
some inflammatory markers could be a predictive factor for clinical onset of acute coronary
syndrome, and also for the clinical outcome of ACS. Serum levels were measured for the following
inflammatory markers: interleukin-6 (IL-6), serum amyloid-A (SA-A), high-sensitivity fraction of Creactive protein (hs-CRP), neopterin and fibrinogen - all of which are highly sensitive proteins of acute
inflammatory response, and their serum levels could be potential predictors of extent and outcome of
acute coronary syndrome. the study was designed as a prospective study that will include 300
patients with acute coronary syndrome throughout 2 years since the day of admission to the
Coronary care unit - which will be the day of patient's enrolment into the study. First patients was
enroled on February 19th 2004, and last on November 3rd 2004. Criteria for inclusion were: clear
clinical presentation that undisputably shows acute coronary event, characteristic ECG changes, and
significant rise in cardioselective enzymes (CK-MB and Troponin I). The data analysis is still in
progress and relevant results will be published at and appropriate time.
Project Website
Contact Person
Name
Professor Mijo Bergovec
Email
[email protected]
Function
Head of Cardiology Department, University Hospital Dubrava
Phone
+38598228688
Fax
+3852902700
Website
www.kbd.hr
Research Team Objectives
Main Fields
Internal Medicine
Cardiology (invasive-interventional and non-invasive), Internal medicine
Partners/Interests
researcher
Internal Medicine
Academic partners willing to collaborate in a regional or subregional international research project in
the field of carrdiovascular medicine.
Currently we applied for grants for 2 projects to the Croatian ministry of Science, Education and
Sports, and are waiting for results of the call for proposals. Also, we applied for SSA under EU FP6 for
one project, and are also waiting for result.
Internal Medicine
Institution/University
Name of Institution/University
Medical School and Dental School, University of Zagreb
Number of Employees
200
Number of Researchers
100
Country
Croatia
Postal Adress
Av. G. Suska 6
10000 Zagreb
Internal Medicine
Research Team
Research Team Name
Diabetes and Metabolism Researsch Team, Center for
Metabolic Disordes in Diabetes, Institute for Endocrinology,
School of Medicine, University of Belgrade
Individual Researcher
No
Research Team Leader Name
Professor Nebojsa Lalic
Research Team Members
Team Member 0
MD Jelena Seferovic [[email protected]]
Team Member 1
MD Marija Macesic [[email protected]]
Team Member 2
MD, MSc Natasa Rajkovic [[email protected]]
Team Member 3
MD, MSc Tanja Milicic [[email protected]]
Team Member 4
MD, MSc Ljiljana Lukic [[email protected]]
Team Member 5
Assistant Aleksandra Jotic [[email protected]]
Team Member 6
Assistant Katarina Lalic [[email protected]]
Team Member 7
Professor Miroslava Zamaklar [[email protected]]
Research Team Projects
Project Name
Insulin resistance: the role in the pathogenesis of the onset
and progression of neurodegenerative disorders and
atherosclerotic vascular disease
Project Leader
Professor Nebojsa Lalic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Serbia / Institute for Neurology, School of Medicine, University of
Belgrade Serbia / Institute for Cardiovascular Diseases, School of
Medicine, University of Belgrade
Project Summary
It has been previously suggested that insulin resistance (IR) exerts an important role in the
pathogenesis of both neurodegenerative disorders (NDD) (Alzheimer, Parkinson and Huntington
diseases) and atherosclerotic vascular disease (AVD) (ischemic brain, ischemic heart and peripheral
vascular diseases). However, both differences and similarities have been reported in the pathogenetic
role of IR. In AVD IR induces vascular atherogenesis, while in NDD it potentially exhibits a dual effect:
an inhibition of insulin-mediated intracellular metabolism in the brain and an induction of vascular
atherogenesis. Those IR-mediated effects might be determined by adipocytokine levels. In this
project, the primary objective is a comparative analysis of the relationship between IR and the onset
and progression of both NDD and AVD. The secondary objectives include the evaluation of
relationship between IR and (1) vascular atherogenic risk factors (endothelial dysfunction, glucose
intolerance, dyslipidemias, hypofibrinolysis, inflammatory mediators, total and abdominal obesity and
arterial pressure) in AVD; (2) inhibition of insulin action in the brain in NDD; (3) vascular atherogenic
risk factors in NDD; (4) insulin and adipocytokine (adiponectin, resistin and leptin) levels in NDD; (5)
insulin and adipocytokine levels in AVD. Thus, this research project will provide the design to clarify
the pathogenetic relevance of different mechanisms involving IR detectable in NDD and AVD.
Project Website
www.mntr.sr.gov.yu
Contact Person
Name
Professor Nebojsa Lalic
Email
[email protected]
Function
Head of the Center for Metabolic Disorders in Diabetes
Phone
+381113616317
Fax
+381112685393
Website
http://www.med.bg.ac.yu/
Internal Medicine
Research Team Objectives
Main Fields
Internal Medicine
Immunology and Immunohaematology
Therapeutics
Insulin resistance, insulin secretion impairements, type 2 diabetes, obesity, prevention of type 2
diabetes, chronic vascular complications of diabetes, type 1 diabetes, prediction and prevention of
type 1 diabetes, new oral agents and insulin formulations in diabetes treatment, insulin pumps, islet
and pancreas transplantation.
Partners/Interests
researcher
industrial partner
Internal Medicine
Immunology and Immunohaematology
Therapeutics
Experience in the field of diabetes and metabolism research and compatibility of interest.
Institution/University
Name of Institution/University
School of Medicine, University of Belgrade
Number of Employees
1000
Number of Researchers
850
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgrade
Internal Medicine
Research Team
Research Team Name
Endocrine Obesity
Individual Researcher
No
Research Team Leader Name
Professor Dragan Micic
Research Team Members
Team Member 0
MD Danica Stamenkovic-Pejkovic [[email protected]]
Team Member 1
Ass. Professor Mirjana Sumarac-Dumanovic [[email protected]]
Team Member 2
MD Goran Cvijovic [[email protected]]
Team Member 3
Associate Professor Aleksandra Kendereski [[email protected]]
Team Member 4
Professor Dragan Micic [[email protected]]
Research Team Projects
Project Name
Endocrine regulatory mechanisms in metabolic disorders
Project Leader
Professor Dragan Micic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-01
Project End Date
2010-12-31
Project Partners
Project Summary
The aim of the project is to evaluate model secretion of ghrelin, insulin resistance, adiponectin and
CRP in metabolic disorders such as: obesity, metabolic syndrome X and PCOS. These investigations
will be performed in basal conditions as well after the performance of adequate therapeutic
interventions. These procedures would be performed in regular time intervals: 0.3 and 6 months.
Project Website
www.mntr.sr.gov.yu
Research Team Projects
Project Name
The role of new secretagogues in control of growth hormone
secretion in endocrine disorders
Project Leader
Professor Dragan Micic [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2002-01-01
Project End Date
2005-12-31
Project Partners
Project Summary
During investigation perid we tested the role of new growth hormone secretagogues in different
endocrine disorders and results were published in international journals 1: Popovic V, Pekic S, Micic
D, Damjanovic S, Marikovic J, Simic M, Dieguez C, Casanueva FF. Evaluation of the reproducibility of
the GHRH plus GHRP-6 test of growth hormone reserve in adults. Clin Endocrinol (Oxf). 2004
Feb;60(2):185-91. 2: Micic D, Kendereski A, Sumarac-Dumanovic M, Cvijovic G, Popovic V, Dieguez
C, Casanueva F. Growth hormone response to GHRH + GHRP-6 in type 2 diabetes during euglycemic
and hyperglycemic clamp. Diabetes Res Clin Pract. 2004 Jan;63(1):37-45. 3: Camina JP, Carreira MC,
Micic D, Pombo M, Kelestimur F, Dieguez C, Casanueva FF. Regulation of ghrelin secretion and action.
Endocrine. 2003 Oct;22(1):5-12. Review. 4: Popovic V, Pekic S, Doknic M, Micic D, Damjanovic S,
Zarkovic M, Aimaretti G, Corneli G, Ghigo E, Deiguez C, Casanueva FF. The effectiveness of arginine
+ GHRH test compared with GHRH + GHRP-6 test in diagnosing growth hormone deficiency in adults.
Clin Endocrinol (Oxf). 2003 Aug;59(2):251-7. 5: Popovic V, Miljic D, Micic D, Damjanovic S, Arvat E,
Ghigo E, Dieguez C, Casanueva FF. Ghrelin main action on the regulation of growth hormone release
is exerted at hypothalamic level. J Clin Endocrinol Metab. 2003 Jul;88(7):3450-3. 6: Micic D,
Sumarac-Dumanovic M, Macut Dj, Kendereski A, Zoric S, Popovic V, Cvijovic G, Dieguez C,
Casanueva FF. Growth-hormone response to combined stimulation with GHRH plus GH-releasing
peptide-6 in obese patients with polycystic ovary syndrome before and after short-term fasting. J
Endocrinol Invest. 2003 Apr;26(4):333-40.
Internal Medicine
Project Website
www.mntr.sr.gov.yu
Contact Person
Name
Professor Dragan Micic
Email
[email protected]
Function
Head of the Center for the Metabolic Disorders in Endocrinology
Phone
381 11 2 656 527
Fax
381 2 685 357
Website
med.bg.ac.yu
Research Team Objectives
Main Fields
Internal Medicine
Obesity, PCOS, Metabolic Syndrome, Insulin Resistance, Type 2 Diabetes Mellitus
Partners/Interests
researcher
Endocrinologist, immunologist
Internal Medicine
We are interested in investigation about possible interplay between ghrelin/leptin/adiponectn/visfatin
and Il-cytokine axis. in various autoimune/inflammatory conditions and low grade inflammatory
conditions.
Institution/University
Name of Institution/University
School of Medicine, University of Belgrade
Number of Employees
800
Number of Researchers
500
Country
Serbia and Montenegro
Postal Adress
Dr Subotica 8
11000 Belgrade
Internal Medicine
Research Team
Research Team Name
Infectious agents involved in vessel aging
Individual Researcher
No
Research Team Leader Name
Prof. MD Carmen Ardeleanu
Contact Person
Name
Prof. MD Carmen Ardeleanu
Email
[email protected]
Function
Head of the Pathology Department
Phone
+40 21 3192734
Fax
+40 21 3192734
Website
Research Team Objectives
Main Fields
Internal Medicine
Translating research for human health virus, bacteria, vessels, atheroma, sclerosis, aging
Partners/Interests
researcher
Internal Medicine
Objectives: To identify the potential relationship between infectious (viral and bacterial) agents and
structural changes in normal or pathological vessel aging. Expected results: Quantification of
infectious vascular loading related to structural changes of various cellular types of the
microenvironment. Impact: Affordable prevention and treatment of vascular degenerative diseases
based on antibiotics or immunotherapy. Contribution to the EU or regional policy: The project´s
results will contribute to obtain new knowledges in elucidating the pathogenesis of degenerative
changes of vessels in aging and in preventing vascular pathologic aging by means of antiinfectious
therapy. European research potential: The actual project may constitute a solid base for
improvements of clinical outcomes, with an establishment of standards for normal and pathological
structural modifications of vessels in normal and pathological aging. The results may be converted
into social and economic benefits of prevention and advanced age disease as vascular cerebral or
cardiac diseases. European added value: By creating a multidisciplinary research on vascular aging
the project may become a base of an European centre of excellence through collaborative research.
Institution/University
Name of Institution/University
"Victor Babes" National Institute of Pathology
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Romania
Postal Adress
Spl. Independentei 99-101
50096 Bucharest
Internal Medicine
Research Team
Research Team Name
Internal Medicine
Individual Researcher
No
Research Team Leader Name
Prof. MD, PhD Breda Pecovnik Balon
Research Team Members
Team Member 0
mag. Maksimiljan Gorenjak []
Team Member 1
mag. Robert Ekart []
Team Member 2
Prof Radovan Hojs [[email protected]]
Contact Person
Name
Prof. Breda Pecovnik Balon
Email
[email protected]
Function
Head Dpt
Phone
+38623212484
Fax
+38623312393
Website
uni.mb
Research Team Objectives
Main Fields
Internal Medicine
Atherosclerosis, Hemodialysis patients, renal osteodistrophy
Partners/Interests
researcher
hemodialysis patients with vascular calcifications
Internal Medicine
Radiology
CARDIOVASCULAR CALCIFICATIONS IN PATIENTS ON HEMODIALYSIS Calcification is a widespread
pathologic process in patients with end-stage renal disease. The most frequent and also clinically
most significant are vascular and valvular calcifications. The risk factors are: hypertension, diabetes,
hyperlipidemia, smoking, patient age, duration of hemodialysis treatment, hypercalcemia and
hyperphosphatemia, receiving of Ca-containing P binders, hyperhomocysteinemia and increased
serum fibrinogen, CRP or low albumin levels. Various noninvasive methods have been used to assess
and quantify vascular and valvular calcification. Two methods are used most frequently: electron
beam computed tomography (EBCT) and multisplice spiral CT. Vascular calcification is also associated
with poor outcome in patients with ESRD. It is known that previous myocardial infarction, stenocardia
or known coronary disease is associated with increased vascular calcification. It was found a strong
association between the presence and extent of vascular calcifications and all-cause and
cardiovascular mortality in ESRD patients. However, the fact that inflammation is a protagonist of
vascular calcification has been well proved. The impact of inflammation on osteoporosis and vascular
calcification can explain the correlation between increased vascular calcification and decreased bone
density in dialysis patients. There is also the influence of inhibitors of calcification. One of them is
fetuin-A or alfa2-Heremans-Schmid glycoprotein (AHSG). In patients on dialysis treatment the
concentration of fetuin-A is lower than in individuals of corresponding sex and age without renal
disease. In our research program we will examine hemodialysis patients. We want to demonstrate the
influence of risk factors on vascular calcifications (measured by spiral CT) and bone density
(measured by DEXA and CT). We will also measured biomarkers of vascular calcifications, including
fetuin - A and biochemical markers of inflamation (CRP). We are also interested in prevention of
vascular calcification with calcimimetics, sevelamer or analogues of vitamin D. Biphosphonates may
also affect the development of calcifications by way of decreasing Ca and P concentrations. Statins
can also have a beneficial effect on calcification by decreasing lipid levels.
Institution/University
Name of Institution/University
University Maribor
Number of Employees
400
Number of Researchers
20
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Internal Medicine
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Prof Neđad Mulabegović MD PhD
Contact Person
Name
Neđad Mulabegović MD, PhD
Email
[email protected]
Function
Head of the Institute of Pharmacology, Clinical Pharmacology and
Toxicology
Phone
+33 663 742 / 743
Fax
+33 203 670
Website
www.mf.unsa.ba
Research Team Objectives
Main Fields
Internal Medicine
Partners/Interests
THE IMPACT OF ACE INHIBITORS USE ON MARKERS OF INFLAMMATION AND OXIDATIVE
STRESS IN THE METABOLIC SYNDROME
BACKGROUND: Individuals with 3 or more of the following clinical features: abdominal obesity,
abnormal glucose, low HDL, hypertriglyceridemia and hypertension, have metabolic syndrome.
Findings of recent studies have suggested that proinflammatory and prooxidative processes have a
significant role in the development and progression of cardiovascular diseases. The renin-angiotensin
system (RAS) has a significant role in the pathogenesis of atherosclerosis-related diseases.
Angiotensin II is a central molecule in the RAS, and it has numerous effects on uptake, synthesis and
oxidation of lipids, inflammation, atherosclerotic plaque formation and progression.
OBJECTIVE: To determine whether use of angiotensin-converting enzyme (ACE) inhibitor have impact
on markers of inflammation and oxidative stress in subjects with the metabolic syndrome.
RESEARCH DESIGN AND METHODS: subjects with at least three out of five diagnostic criteria for
metabolic syndrome will be enrolled in the study. Subjects will be randomized in a double-blinded
fashion to either enalapril 5 mg/day or matching placebo for 12 weeks. Before and after 12 weeks of
enalapril treatment, blood pressure, abdominal girth, serum CRP and 8-isoprostane, fasting HDL
cholesterol, triglycerides and glucose will be measured in all subjects. High-sensitivity CRP will be
measured with the use of Dade Behring CardioPhase hs CRP reagent by laser nephelometry. Serum
8-isoprostane will be measured by IBL enzyme immunoassay kit for the quantitative determination of
STAT – 8 –Isoprostane.
EXPECTED RESULTS: Our results could give contribution to the better understanding of actions and
effects of ACE inhibitors in the metabolic syndrome.
Key words: ACE inhibitors, metabolic syndrome, inflammation, oxidative stress
Institution/University
Name of Institution/University
University of Sarajevo, Faculty of Medicine Sarajevo
Number of Employees
Number of Researchers
Country
Bosnia and Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Internal Medicine
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Prof.dr Emina NAKAŠ-IĆINDIĆ, MD, PhD
Contact Person
Name
Prof Emina Nakas-Icindic, MD PhD
Email
[email protected]
Function
Head of the Department of Human Physiology
Phone
+33 663 742 / 743
Fax
+33 203 670
Website
www.mf.unsa.ba
Research Team Objectives
Main Fields
Internal Medicine
Partners/Interests
Activation of Innate and Adaptive Immune Response by Myocardial Ischemia-Reperfusion
Injury in Animal Model
Background: Reperfusion injury, which enhances the ischemic injury, is common for revascularization
process of myocardial tissue not only in myocardial infarction but also as iatrogenic in open-heart
surgery and balloon dilatation (1). This injury to the heart triggers an intense infiltration of white
blood cells and release of their enzymes and oxidative radicals that injure healthy heart cells that
surround the area of injury. (2) The pathophysiology of ischemia-reperfusion injury and the possible
role of innate and acquired immune system have so far not been well characterized. During ischemia
the heart may produce endogenous ligands, which activate the immune system or may serve as
protective inhibiting leucocytes aggregation. In the heart, there is evidence that toll-like receptors
(3) as well as Nuclear Factor kappa-B (4) are activated in chronic heart failure secondary to
myocardial ischemia. Furthermore, both factors are involved in the development of arteriosclerosis,
where a role immune system is apparent (5).
Aim: The aim of this study is to estimate time and place of activation and the role of some particular
components of innate and adaptive immune system in myocardial ischemia-reperfusion injury and
to identify possible endogenous ligands through studies of cardiomyocytes in culture and co cultures
of cardiomyocytes and peripheral blood mononuclear cells (or others, depending on findings above).
Methods and techniques: The experiments will be conducted in vivo (mice), isolated heart and in
tissue cultures. Immunohistochemistry of myocardial tissue will be used to evaluate cell infiltration in
scar tissue and cellular localization, analysis of activation of circulating white blood cell populations
with flow cytometry. Immunoblotting of myocardial protein extracts to evaluate activation of toll-like
and NOD receptors, and mitogen activated protein kinases, as well as studies of gene expression in
extracts of hearts and circulating cells of RNA corresponding to the proteins found. Studies of
cardiomyocytes in culture and co cultures of cardiomyocytes and peripheral blood mononuclear cells
(or others, depending on findings above) are needed to identify some possible endogenous ligands.
After getting good quantity and quality of isolated mouse cardiomyocytes, signal transduction
pathways after different stimuli such as anoxia, cytokine stimulation, and heat shock proteins will be
tested.
Expected results The research on innate and adaptive immune system activation in response to
myocardial ischemia-reperfusion injury in animal model could help to elicit the role of innate and
adaptive immunity in humans in order to maximize tissue recovery and to reduce collateral damage.
References:
1. “A review of leukofiltration in cardiac surgery: the time course of reperfusion injury may facilitate
study design of anti-inflammatory effects”, Hodder Arnold Journals, Perfusion, Volume 17,
Supplement 1, 1 May 2002, pp 53-62.
2. Lindsey M. Et Al. “Matrix-dependent mechanism of neutrophil-mediated release and activation of
matrix metalloproteinase 9 in myocardial ischemia-reperfusion. “, Circulation 103:2181-2187, 2001.
3.Toll-like receptor 4 mediates ischemia/reperfusion injury of the heart Chong et al. J Thorac
Cardiovasc Surg.2004; 128: 170-179
4.Valen G, Yan Z-Q, Hansson GK. Nuclear Factor kappa-B and the heart. Journal of American College
of Cardiology 2001;38;307-314.
5. Hansson GK. Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med
2005;352:1685
Internal Medicine
Institution/University
Name of Institution/University
University of Sarajevo, Faculty of Medicine Sarajevo
Number of Employees
Number of Researchers
Country
Bosnia and Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Internal Medicine
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Dr sci. Elma KUČUKALIĆ-SELIMOVIĆ
Research Team Members
Team Member 0
Beslic S.
Team Member 1
Basic H.
Team Member 2
Beslic N.
Team Member 3
Bilalovic N.
Team Member 4
Secic S.
Team Member 5
Beslija S.
Team Member 6
Milardovic R.
Team Member 7
Kapetanovic E.
Team Member 8
Tatarevic A.
Team Member 9
Hadzimuratovic S.
Team Member 10
Balta E.
Team Member 11
Mahic N.
Team Member 12
Begic A.
Team Member 13
Muftic K.
Contact Person
Name
Dr sci. Elma KUČUKALIĆ-SELIMOVIĆ
Email
[email protected]
Function
docent, Department of Internal Medicine and Clinics of
Propaedeutics
Phone
+33 663 742 / 743
Fax
+33 203 670
Website
www.mf.unsa.ba
Research Team Objectives
Main Fields
Internal Medicine
Partners/Interests
Evaluation of the SLN Biopsy Impact on Incidence and Localisation of the Recurant
Desease in Patients with Breast Cancer
The current standard approach to determine the spread of breast cancer is an ALD (axillary lymph
node dissection), the surgical procedure that carries with it risk of postoperative morbidity. SLN
(sentinel lymph node) biopsy is reliable method in detecting the spread of breast cancer which can
enable ALD to be avoided and that is why this method is considered as important in the management
of breast cancer patients. Crucial parameter concerning the accuracy of SLN biopsy in breast cancer
surgery is the impact of this procedure on the long term clinical outcome of patients.
AIM: To evaluate correlation of both tumour size and tumour type in regard to negative SLN on the
development of the recurrent disease and to define the impact of this procedure on the long-term
clinical outcome of the patients.
PATIENTS AND METHODS:
•
The study would be multidisciplinary, prospective randomised trial
including patients with operable breast carcinoma and clinically negative
axillary lymph
nodes (T 1-2 N0 M0).
•
Patients should be referred for lymph node scintigraphy prior to surgery.
•
On the surgery, on base of the SLN intraoperative frozen section hystopatological result,
operation will be adequately completed.
•
Only SLN negative patients should be included in the trial.
•
Oncologycal treatment will follow the recommended protocol for curtain cases.
Internal Medicine
•
The data should be analysed after 2 and 4 years long follow up.
EXPECTED RESULTS: It is very likely that T1 breast cancer with a negative SLN will not develop
tumour recurrence in proposed follow up period. But, influence of negative SLN on disease free period
is not completely clear and we would like to contribute clarification of this issue which is very
important in sense of predictability of disease outcome. In this regard performance of SLN biopsy
without subsequent axillary clearance requires adequate follow up.
LITERATURE:
1. Mariani G, et al. Radioguided Sentinel Lymph Node Biopsy in Brest Cancer Surgery. Eur J Nucl
Med. 2001; 42; 1198-1213.
2. Veronesi U,Paganelli G, Galimberti V, et al. Sentinel node biopsy to avoid axillary dissection in
breast cancer with clinically negative lymph nodes. Lancet 1997; 349: 1864-1867.
3. Veronesi U, Luini A, Galimberti V, Marchini S, Sacchini V, Rilke F. Extent of metastatic axillary
involvment in 1446 cases of breast cancer. Eur J Surg Oncol. 1990;16:127-133.
4. Djulbegovic B., D.M.Sullivan Decision Making in Oncology Evidence Based Management.
Churchill Livingstone, 1997; 253 –275.
Institution/University
Name of Institution/University
University of Sarajevo, Faculty of Medicine Sarajevo
Number of Employees
Number of Researchers
Country
Bosnia and Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Internal Medicine
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Prof, MD PhD Bakir Mehić
Contact Person
Name
Prof, MD PhD Bakir Mehić
Email
[email protected]
Function
Vice Dean
Phone
+38733444343
Fax
+38733272691
Website
mf.unsa.ba
Research Team Objectives
Main Fields
Internal Medicine
Partners/Interests
THE PRECANCEROUSES OF BRONCHIAL MUCOSA IN SMOKERS AND COPD PATIENTS IN
SOUTHEAST EUROPEAN COUNTRIES
Background: The identification of abnormal or suspicious areas in tracheobronchial tree by
autofluorescence bronchoscopy (AFB) is possible, even the white light bronchoscopy (WLB)
examination stay as a normal.
Bronchial lesions in smokers and patients with different stages of COPD depend from duration and
quantity of the exposition to the risk factors.
Main goals:
To determine the frequency of metaplasia, dysplasia and carcinoma in particular stages of
COPD in Southeast European countries.
To compare the duration of exposure to risk factors with histological picture of metaplasia,
dysplasia and carcinoma in Southeast European countries.
To compare the COPD stages with the frequency of histological picture of metaplasia,
dysplasia and carcinoma.
Methods: Both, WLB and AFB will be performing with the aim of detection of precancerous and
cancerous bronchial lesions in patients with different stages of COPD (by GOLD). The biopsy
specimens will be taken in areas of tracheobronchial tree judged as abnormal or suspicious at WLB
and/or AFB.
Histological data will be comparing with the stage of COPD as well as with duration and quantity of
exposure to risk factors (smoking, professional pollution etc).
Anticipated results: There is very similar prevalence of smoking in Southeast countries. The question
is: are there significant differences between the consequences of smoking in separate country
centers?
Institution/University
Name of Institution/University
Medical Faculty University of Sarajevo
Number of Employees
382
Number of Researchers
104
Country
Bosnia-Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Internal Medicine
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Hellmut Samonigg
Contact Person
Name
Prof. Dr. Hellmut Samonigg
Email
[email protected]
Function
vice dean for strategy and innovation, head of the department of
oncology
Phone
+43 316 385 3115
Fax
+43 316 385 4167
Website
http://www.onkologie-graz.at/
Research Team Objectives
Main Fields
Internal Medicine
Oncology
Cancer
Partners/Interests
Oncology
Cancer
Institution/University
Name of Institution/University
Clinical Department of Oncology, Internal Medicine, Medical
University of Graz
Number of Employees
Number of Researchers
Country
Austria
Postal Adress
Auenbruggerplatz 15
8036 Graz
Microbiology
Research Team
Research Team Name
Department of Microbiology
Individual Researcher
No
Research Team Leader Name
MD, PhD Maja Abram
Research Team Members
Team Member 0
MD, PhD Darinka Vuckovic [[email protected]]
Team Member 1
MD, PhD Tomislav Rukavina [[email protected]]
Team Member 2
MD, PhD Brigita Ticac [[email protected]]
Team Member 3
MD, MSc Marina Bubonja [[email protected]]
Team Member 4
MD, PhD Miljenko Doric [[email protected]]
Team Member 5
Sanit.Eng., PhD Marina Santic [[email protected]]
Team Member 6
MD Roberta Rubesa-Mihaljevic [[email protected]]
Team Member 7
MD Ivana Skrobonja [[email protected]]
Team Member 8
Sanit. Eng. Vanja Marchesi [[email protected]]
Team Member 9
Sanit.Eng, MSc Ivana Gobin [[email protected]]
Team Member 10
MD Mirela Markanovic [ [email protected] ]
Research Team Projects
Project Name
Experimental Listeria monocytogenes infection during
pregnancy
Project Leader
prof., MD, PhD Maja Abram [[email protected]]
Project Funding Agency
Croatian Ministry of Science
Project Budget
10.000 €
Project Start Date
2002-07-01
Project End Date
2006-12-31
Project Partners
Germany, University of Cologne, Dpt. Neuropathology Slovenia,
University of Ljubljana, Institute of Microbiology and Immunology
Project Summary
Investigation of cellular and molecular mechanisms which are of importance for the pathogenesis of
congenital listeriosis; determination of specific modulation in the cytokine/chemokine production at
the systemic and local level; using animal model, recovery from listeriosis, resistance mechanisms,
the fetal and maternal contribution to the cytokine/chemokine milieu are followed.
Project Website
Project Name
Resistance and virulence of Campylobacter
Project Leader
prof. MD, PhD Maja Abram [[email protected]]
Project Funding Agency
Croatian and Slovenian Ministry of Science
Project Budget
2.000 €
Project Start Date
2004-01-01
Project End Date
2005-12-31
Project Partners
Slovenia, University of Ljubljana, Biotechnical Faculty, Dpt.of
Microbiology
Project Summary
In vitro evaluation of viability and virulence of experimentally stressed Campylobacter jejuni; ability
of Campylobacter jejuni to invade and survive in mammalian cells in vitro; Host resistance to primary
and secondary Campylobacter jejuni infections in C57Bl/6 mice.
Project Website
Project Name
Pathogenesis of experimental legionellosis
Project Leader
prof, MD, PhD Miljenko Doric [[email protected]]
Project Funding Agency
Croatian Ministry of Science
Microbiology
Project Budget
15.000 €
Project Start Date
2002-07-01
Project End Date
2006-12-31
Project Partners
Austria, University of Graz, Institute for Hygiene USA, University of
Louisville, Dpt. Microbiology
Project Summary
Using an experimental model on A/J mice histopathology of Legionnaires' disease and the role of
different T cell subsets were analyzed; the differences in the cytopathogenic capacity of different
Legionella species are investigated.
Project Website
Project Name
Antilipopolisacharide immunity during Klebsiella infection
Project Leader
prof, MD, PhD Tomislav Rukavina [[email protected]]
Project Funding Agency
Croatian Ministry of Science
Project Budget
18.000 €
Project Start Date
2002-07-01
Project End Date
2006-12-31
Project Partners
Project Summary
Determination of cell surface receptor signals involved in anti-LPS mediated protection in an animal
model of systemic Klebsiella infection; cytokine pattern associated with the survival of animals
protected with anti-LPS antibodies.
Project Website
Contact Person
Name
MD, PhD Maja Abram
Email
[email protected]
Function
Associate professor
Phone
+385 51 651 172
Fax
+385 51 651 177
Website
medr.hr
Research Team Objectives
Main Fields
Microbiology
Immunology and Immunohaematology
Clinical microbiology
Investigation on the pathogenesis of infectious diseases focusing on particular infectious agents: L.
pneumophila, L. monocytogenes, C. jejuni, K. pneumoniae, F. tularensis; host response to bacterial
pathogens; cellular and molecular mechanisms in host-bacteria relationship; a variety of experimental
approaches from animal model, cell cultures to isolated cells are used.
Partners/Interests
researcher
Microbiology
Immunology and Immunohaematology
Clinical microbiology
- cellular microbiology-a molecular understanding of cellular processes that allow microbes to
compete and survive in their host - genotyping of clinical, enivronmental and food isolates
Continuation in studying the properties of the pathogens associated with infectious diseases which
have a significant impact on public health: legionelloses, listeriosis, tularemia, campylobacteriosis and
gram-negative sepsis; Established experimental models (in vivo mouse infection; cell-cultures) will
serve as the basis for our future studies on the properties of pathogens, as well as, investigation of
the host's susceptibility to infection and the ability of the immune system to control or eliminate the
microorganisms.
Institution/University
Name of Institution/University
Dpt. Microbiology, Medical Faculty, University of Rijeka
Number of Employees
20
Microbiology
Number of Researchers
11
Country
Croatia
Postal Adress
Brace Branchetta 20
51000 Rijeka
Microbiology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
prof. dr. Stanislav Šuškovič, dr.med., spec
Contact Person
Name
prof. dr. Stanislav Šuškovič, dr.med., spec
Email
[email protected]
Function
Phone
+386 4 25 69 383
Fax
+386 4 25 69 117
Website
http://www.klinika-golnik.si/
Research Team Objectives
Main Fields
Microbiology
Immunology
Partners/Interests
Microbiology
Immunology
Epidemiologic data of COPD or asthma are badly needed in Slovenia. We intend to perform relevant
epidemiological studies in cooperation with the experts from the Institute of Public Health of the
Republic of Slovenia.
Institution/University
Name of Institution/University
University Clinic for Respiratory and Allergic Diseases Golnik
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Golnik 36
4204 Golnik
Microbiology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
dr. Peter Korosec
Contact Person
Name
dr. Peter Korosec
Email
[email protected]
Function
Phone
+386 4 25 69 100
Fax
+386 4 25 69 117
Website
http://www.klinika-golnik.si/
Research Team Objectives
Main Fields
Microbiology
Immunology
Partners/Interests
Microbiology
Immunology
We would be pleased to collaborate with those who might be interested in this topic.
Expression of COX-1 and -2 in monocytes of aspirin-int
olerant(AI) patients
The aim of our study is to find out whether it would be possible to identify aspirin-intolerant patients
by measuring the expression of COX 1 and/or 2 in peripheral-blood monocytes, therefore without
clinical provocation.
We incubate samples of AI patients (and controls) venous blood with either 1.nothing added, 2. LPS
added (activation), 3. LPS + aspirin added and 4. only aspirin added. We then lyse erythrocytes and
stain COX-1 and 2 by intracellular staining with marked monoclonal antibodies. Monocytes are also
marked with anti-CD 14 antibodies. Levels of expression are measured by flow cytometry. Our
hypothesis is that COX-1 expression remains invariated in all cases, and that COX-2 expression in
LPS-stimulated (but not unstimulated) monocytes exposed to aspirin in AI patients is significantly
higher than the level of COX-2 expression in healthy controls.
Institution/University
Name of Institution/University
University Clinic for Respiratory and Allergic Diseases Golnik
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Golnik 36
4204 Golnik
Microbiology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
dr. Marc Malovrh Mateja
Research Team Members
Team Member 0
Sabina Skrgat [[email protected]]
Contact Person
Name
dr. Marc Malovrh Mateja
Email
[email protected]
Function
Phone
+386 4 25 69 100
Fax
+386 4 25 69 117
Website
http://www.klinika-golnik.si/
Research Team Objectives
Main Fields
Microbiology
Immunology
Partners/Interests
Microbiology
Immunology
The in vivo and in vitro results propose the involvement of anaphylatoxins and angiogenetic factors in
the pathogenesis of COPD. Our aim is to determine the levels of anaphylatoxins (C5a, C4a, and C3a)
and angiogenetic factors (VEGF, angiogenin, bFGF, IL8, TNF) at the site of inflammation in stable
COPD (concentrating on the correlation of C5a or angiogenetic factors concentrations with the
extensity of emphysema), in exacerbation of a disease and in healthy smokers. We measure the
concentrations of anaphylatoxins and angiogenetic factors in the induced sputum by cytometric bead
array method (CBA).
Institution/University
Name of Institution/University
University Clinic for Respiratory and Allergic Diseases Golnik
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Golnik 36
4204 Golnik
Neurology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Professor, MD, PhD Marin Stancic
Contact Person
Name
Professor, MD, PhD Marin Stancic
Email
[email protected]
Function
Dept. Chief, Universiyt Hospital of Traumatology Zagreb
Phone
+385 1 46 97 139
Fax
+385 1 46 10 365
Website
www.trauma.hr
Research Team Objectives
Main Fields
Neurology
Surgery
Anatomy
1. anatomy and regeneration of peripheral nerves 2. biomechanics of the spine
Partners/Interests
researcher
Anatomy
Surgery
Psychiatry
interested in collaboration in the projects in the indicated scientific fields
anatomy and regeneration of peripheral nerves biomechanics of the spine
Institution/University
Name of Institution/University
University Hospital of Traumatology Zagreb
Number of Employees
452
Number of Researchers
18
Country
Croatia
Postal Adress
Draskoviceva 19
10000 Zagreb
Neurology
Research Team
Research Team Name
Group for Neurodegenerative Disorders (Institute of
Neurology CCS, Medical School Belgrade)
Individual Researcher
No
Research Team Leader Name
Prof Kostić Vladimir
Research Team Members
Team Member 0
Assoc Prof Svetel Marina [[email protected]]
Team Member 1
Assoc Prof Stefanova Elka [[email protected]]
Team Member 2
Assist Prof Dragašević Nataša [[email protected]]
Team Member 3
dr med Petrović Igor [[email protected]]
Team Member 4
Assoc Prof Marković Ivanka [[email protected]]
Team Member 5
Associ Prof Novaković Ivana [[email protected]]
Team Member 6
Researcher Radovanović Saša [[email protected]]
Team Member 7
Junior Researcher Marić Jelena [[email protected]]
Team Member 8
Prof Pekmezović Tanja [[email protected]]
Team Member 9
Prof Kostić Vladimir [[email protected]]
Contact Person
Name
Prof Kostić Vladimir
Email
[email protected]
Function
Head, Institute of Neurology CCS
Phone
381-11-2685596
Fax
381-11-2684577
Website
www.med.fak.bg.ac.yu
Research Team Objectives
Main Fields
Neurology
Genetics
Epidemiology
1. Genetics of movement disorders (Parkinson'disease, dystonia, Wilson's disease, spinocerebellar
ataxia) and genotype/phenotype correlations 2. Non-motor problems in Parkinson's disease and
atypical parkinsonism (MSA, PSP, vascular parkinsonism), particularly depression and dementia 3.
Epidemiology of dystonia, parkinsonism, spinocerebellar ataxia and other neurodegenerative
disorders
Partners/Interests
researcher
Genetics
Physiology
Neurology
1. possibilities for mor advanced genetical investigation and expertise in degenerative disorders
1. Investigation of the possible genetic and biochemical, as well as clinical correlates of non-motor
symptoms in Parkinson's disease and parkinsonism 2. Genotype/phenotype correlation in
neurodegenerative disorders
Institution/University
Name of Institution/University
Medical School University of Belgrade
Number of Employees
900
Number of Researchers
450
Country
Serbia and Montenegro
Postal Adress
ul. dr Subotića 8
11000 Belgrade
Obstetrics and Gynaecology
Research Team
Research Team Name
Reproductive Medicine and Gynecologic Endocrinology
Individual Researcher
No
Research Team Leader Name
Mr. MD, PhD Veljko Vlaisavljevic
Research Team Members
Team Member 0
Mr. MD, PhD Veljko Vlaisavljevic [[email protected]]
Team Member 1
Mr., MD, PhD Borut Kovacic [[email protected]]
Contact Person
Name
Mr. MD, PhD Veljko Vlaisavljeviæ
Email
[email protected]
Function
Head of Department of Reproductive Medicine and Gynecologic
Endoc
Phone
+386 2 321 24 89
Fax
+386 2 331 23 93
Website
http://www.ivf-mb.net
Research Team Objectives
Main Fields
Obstetrics and Gynaecology
Reproductive Medicine and Gynecologic Endocrinology
Partners/Interests
researcher
Suitable
Obstetrics and Gynaecology
Chromosomal abnormalities in embryos from natural and IVM cycles and identification of specific
proteins characteristic for human blastomere and stem cells diferentiation. The cells of early
preimplantation embryos are totipotent (autonomous capability to develop to the embryo). The loss
of some cells from the early embryo does not threats its vitality. This embryo ability is exploited in
many assisted reproductive techniques. In preimplantation genetic diagnosis (PGD) one or two cells
are taken from 8 cell embryo for genetic analysis. In embryo freezing also happens frequently that
some blastomeres do not survive the procedure.. New discoveries on mouse embryos prove, that only
the cells from 2 – 4 cell embryos are totipotent. Later stages have the blastomeres still
predetermined for the diferentiation into extra-embryonic and embryonic cell lines. This was
confirmed by the presence of Oct-4 in the cells, protein which is known only for pluripotent embryonic
cell line.. The embryos obtained by the procedure of in vitro maturation (IVM) of oocytes, have more
nuclear abnormalities and also more frequently arrest in the development than the embryos from
classic in vitro fertilization (IVF) procedure . The frequency of such abnormalities in embryos from
natural IVF cycle is not known. From this reason it prevails the opinion, that before the
embryotransfer these embryos should be screened for aneuploidies by PGD. The knowledge about the
stage in which the blastomeres are still totipotent, should enable, that PGD will be performed before
the cell diferentiation. In such a manner we could be sure that the embryo will not be additionally
affected by the biopsy of blastomeres. Anticipated results : Aim of the study is to ascertain the details
about the frequeny of chromosomal abnormalities in embryos coming from natural and IVM cycles
and ascertain the beginning of human blastomere differentiation The confirmation of OCT-4 protein
also in the human 8-cell embryos would mean, that the blastomeres of 8-cell embryo are still
differentiated. This information could cause the redefinition of the safety of some assisted
reproductive technologies, which exploite individual cells for the diagnosis. Some cells from clinically
not utilized human embryos from an IVF/ICSI programe, which arrest in various stages (2, 4, 8, >8
cell stage), will be analyzed for the expression of genes, responsible for the differentiation or for
pluripotency. The stage in which the human embryo starts to differentiate will be determined. These
informations are important for reproductive medicine, because the embryo stage in which the biopsy
of blastomeres is still acceptable, must be exactly known. The results will be helpfull for the
estimation of the potential of embryonic cells for the derivation of embryonic stem cells.
Institution/University
Name of Institution/University
Maribor Teaching Hospital
Number of Employees
2400
Number of Researchers
33
Obstetrics and Gynaecology
Country
Slovenia
Postal Adress
Ljubljanska 5
2000 Maribor
Other Allied Sciences
Research Team
Research Team Name
Institute of Forensic Medicine and Criminology, Medical
School University of Zagreb, Croatia
Individual Researcher
No
Research Team Leader Name
Professor, MD, PhD Davor Strinovic
Research Team Members
Team Member 0
MD, PhD, Assist.Prof Milovan Kubat [[email protected]]
Team Member 1
PhD, Assoc. Prof. Mario Slaus [[email protected]]
Team Member 2
MD, PhD, Senior Ass. Vedrana Petrovecki [[email protected]]
Team Member 3
MD, MSc, Junior Ass. Davor Mayer [[email protected]]
Contact Person
Name
Professor, MD, PhD Davor Strinovic
Email
[email protected]
Function
Professor of Forensic Medicine
Phone
+385 1 4566 827
Fax
+385 1 4590 221
Website
www.mef.hr
Research Team Objectives
Main Fields
Other allied sciences
Work in the field of Forensic Medicine. Identification process of recovered human remains.
Establishing anthropometric (age, gender, height) and medicolegal (injuries, cause of death)
characteristics of exhumated individuals. Identification of the deceased using "classical" methods in
forensic medicine as well as forensic genotyping (DNA anaysis). Dealing with victims of mass
disasters in civil circumstances (e.g. traffic accidents).
Partners/Interests
researcher
Other allied sciences
Partners of the corresponding profiles.
Projects focused on identification of victims in mass disasters.
Institution/University
Name of Institution/University
Dept of Forensic Medicine, Medical School University of Zagreb
Number of Employees
25
Number of Researchers
6
Country
Croatia
Postal Adress
Salata 11
10000 Zagreb
Pathology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
MD PhD Marin Nola
Research Team Members
Team Member 0
MD Alinda Varnai []
Team Member 1
Prof, MD, PhD, Ivan Damjanov []
Team Member 2
Mr sci Tomièic Ivana []
Team Member 3
MD, Mr sci Blažanovic Anto []
Team Member 4
Mr sci Koraljka Gjadrov []
Team Member 5
Mr sci Mirela Mirt []
Team Member 6
MD, PhD Tvrtko Hudolin []
Team Member 7
Prof, MD, PhD Igor Aurer []
Team Member 8
MD László Pajor []
Team Member 9
MD, PhD Nurija Bilalovic []
Research Team Projects
Project Name
CD 43 - prognostic marker for the outcome of lymphoma
patients
Project Leader
MD, PhD Marin Nola [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-00-00
Project End Date
2011-00-00
Project Partners
Project Summary
CD43 is expressed on the surface of all white blood cells, except on the surface of non-activated
mature B-cells. Simultaneous expression of CD43 and CD20 on B-lymphocytes is considered to be
extremely useful in distinguishing B-cell non-Hodgkin lymphomas from non-neoplastic lymphoid
proliferations. Positive expression of this marker is found in 75-85% of lymphoblastic
lymphomas/leukemias, and some nonlymphoblastic T-cell non-Hodgkin lymphoma such as mycosis
fungoides, non-epidermotropic T-cell lymphoma of the skin, peripheral T-cell lymphoma, and
anaplastic large cell lymphoma. Positive expression of CD43 is also found in 30% of nonlymphoblastic
B-cell, non-Hodgkin lymphomas. That group includes patients with small lymphocytic
lymphoma/chronic lymphocytic leukemia, mantle cell lymphoma, Burkitt lymphoma and diffuse large
B-cell lymphoma. CD43 is positive in 50% of plasmacytomas and multiple myelomas. It is well known
that lymphomas, depending of the mutations, express or lose certain markers on the cell surfaces.
Therefore, expression of CD43 in some types of lymphomas could predict better or poorer outcome of
patients. Second part of the study would investigate connection of certain infections with
development of lymphomas, reaction of patients to therapy an connection of these lymphomas with
CD43. Since a certain number of MALT lymphomas are resistant to therapy and have a tendency of
transformation to diffuse large B-cell lymphoma, it is necessary to recognize that group of patients.
We assume that transformation occurs because of genetic characteristics of certain bacteria, as well
as genetic characteristics of the infected host. Resistance of H. pylori to macrolide antibiotics is very
significant clinically, but there is no research that deals with connection of resistance to macrolides to
MALT lymphomas and DLBCL. Our goal is to establish the occurence of mutations in 23S rRNA region
of H. pylori genome that is responsible for the resistance to macrolides, and possibly use those
mutations as a bacterial marker/marker of MALT lymphomas that undergo transformation to DLBCL.
We would also investigate possible connection between bacterial mutations and Bcl10, MALT1 and
CARMA1 genes of the host. Third part of the study would include flow cytometric and PCR analysis of
DNA, as well as the most common translocations in lymphoid neoplasms, as well as their connection
with CD43 positive as opposed to CD43 negative lymphoid neoplasms.
Project Website
Pathology
Contact Person
Name
MD PhD Marin Nola
Email
[email protected]
Function
Associate Professor
Phone
385-1-2388-089
Fax
385-1-4921-151
Website
none
Research Team Objectives
Main Fields
Pathology
Internal Medicine
hematopathology, hematology, surgical pathology, cytopathology, molecular biology, urology,
Partners/Interests
researcher
industrial partner
Pathology
Internal Medicine
researcher - lymphoma cases stored as paraffin blocks industrial partner - some new antigens for
probe on lymphoma cases
CD43 is expressed on the surface of all white blood cells, except on the surface of non-activated
mature B-cells. Simultaneous expression of CD43 and CD20 on B-lymphocytes is considered to be
extremely useful in distinguishing B-cell non-Hodgkin lymphomas from non-neoplastic lymphoid
proliferations. Positive expression of this marker is found in 75-85% of lymphoblastic
lymphomas/leukemias, and some nonlymphoblastic T-cell non-Hodgkin lymphoma such as mycosis
fungoides, non-epidermotropic T-cell lymphoma of the skin, peripheral T-cell lymphoma, and
anaplastic large cell lymphoma. Positive expression of CD43 is also found in 30% of nonlymphoblastic
B-cell, non-Hodgkin lymphomas. That group includes patients with small lymphocytic
lymphoma/chronic lymphocytic leukemia, mantle cell lymphoma, Burkitt lymphoma and diffuse large
B-cell lymphoma. CD43 is positive in 50% of plasmacytomas and multiple myelomas. It is well known
that lymphomas, depending of the mutations, express or lose certain markers on the cell surfaces.
Therefore, expression of CD43 in some types of lymphomas could predict better or poorer outcome of
patients. Second part of the study would investigate connection of certain infections with
development of lymphomas, reaction of patients to therapy an connection of these lymphomas with
CD43. Since a certain number of MALT lymphomas are resistant to therapy and have a tendency of
transformation to diffuse large B-cell lymphoma, it is necessary to recognize that group of patients.
We assume that transformation occurs because of genetic characteristics of certain bacteria, as well
as genetic characteristics of the infected host. Resistance of H. pylori to macrolide antibiotics is very
significant clinically, but there is no research that deals with connection of resistance to macrolides to
MALT lymphomas and DLBCL. Our goal is to establish the occurence of mutations in 23S rRNA region
of H. pylori genome that is responsible for the resistance to macrolides, and possibly use those
mutations as a bacterial marker/marker of MALT lymphomas that undergo transformation to DLBCL.
We would also investigate possible connection between bacterial mutations and Bcl10, MALT1 and
CARMA1 genes of the host. Third part of the study would include flow cytometric and PCR analysis of
DNA, as well as the most common translocations in lymphoid neoplasms, as well as their connection
with CD43 positive as opposed to CD43 negative lymphoid neoplasms.
Institution/University
Name of Institution/University
Medical University of Zagreb
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Croatia
Postal Adress
Salata 10
10000 Zagreb
Pathology
Research Team
Research Team Name
Improvement of therapeutical approach by molecular
assessment of malignant soft tissue tumors
Individual Researcher
No
Research Team Leader Name
Dr. Cristina Iosif
Contact Person
Name
Dr. Cristina Iosif
Email
[email protected]
Function
Project Director
Phone
+40 21 3192734
Fax
+40 21 3192734
Website
Research Team Objectives
Main Fields
Pathology
soft tissue tumors therapy
Partners/Interests
researcher
Pathology
Malignant soft tissue tumors are rare but usually aggressive and resistant to the classical antitumoral
drugs. The project aims to identify new tumoral or stromal markers as molecular targets for novel
specific therapies. This approach increases the translational character of the basic research.
Objectives: To develop new therapeutic strategies in malignant soft tissue tumors by molecular
characterization of stromal cells and vessels in correlation with activators expression. Expected
results: Improvement of specific antitumor therapy using a new molecular approach. Contribution to
the EU or regional policy: The project will generate new points of view and new approaching policies
about applying novel therapeutic strategies in the management of populational health. The project
may be included in European objective of dynamic and competitive knowledge-based economy.
European research potential: The project realization is ensured by its competitive potential in a
challenging field of molecular based therapies, making possible an excellent translational research
with real economic benefit. European added value: As an optimal research base with multidisciplinary
framework, this project is open to collaborative research with European centers of excellence.
Institution/University
Name of Institution/University
"Victor Babes" National Institute of Pathology
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Romania
Postal Adress
Spl. Independentei 99-101
50096 Bucharest
Pathology
Research Team
Research Team Name
Quantitative analysis and image transmission divission,
Institute of Pathology Medical Faculty University of Zagreb
Individual Researcher
No
Research Team Leader Name
MD, PhD Sven Seiwerth
Research Team Members
Team Member 0
MD Sanja Radeljak [[email protected]]
Team Member 1
MD Vlasta Loncaric []
Team Member 2
MD Natasa Crne []
Team Member 3
MD Luka Brcic [[email protected]]
Team Member 4
MD Lovorka Batelja Vuletic [[email protected]]
Team Member 5
MD, PhD Sven Seiwerth [[email protected]]
Research Team Projects
Project Name
Quantitative analysis and image transmission in pathology
Project Leader
MD, PhD Sven Seiwerth [[email protected]]
Project Funding Agency
Ministry of science, education and sport
Project Budget
25000 €
Project Start Date
2002-10-22
Project End Date
2006-10-22
Project Partners
Project Summary
Pathology is one of the most important diagnostic disciplines giving expertise in everyday routine as
well as in experimental work. Based on pathological diagnosis often additonal chemo or radiation
therapy is planed. Recently, great efforts are made to add more objective elements into the
histopathological diagnosis. This is mostly done by measurements or quantitation (e.g. counting of
positive lymph nods, largest tumor diameter etc.) A further step is made by quantification of
microscopical parameters (nuclear measuremnts, density of nuclei , DNA content etc.). Quantification
on histological slides is stil not widely used and only few parameters are routinely quantitated on this
level. GOAL of this study is to show, on different models, the possibility and usefullness of
morphometrical analysis of histological preparations and to introduce some of them into routine work.
MODELS to be used in this work are: estimation of prognostic walues of immunoreactivity for
cathepsin D, cyclin D1, nm23 in laryngeal cancer tissue, especially with respect to intratumoral
heterogeneity. Flow citometric analysis of the same tissue and LOH analysis will also be performed.
Secondly, morphometric analysis of pigmented skin lesions concerning nuclear parameters and
distribution will be performed using artificial inteligence and machine learning procedures. The studies
are planed as multicentric and international including Zagreb, Slavonski Brod, Amsterdam, Nejmegen,
and Budapest). Thes second part of the project concerns experimental work on animal models of
disease in the study of new peptides and new surgical procedures. This part is closely associated with
other projects of MZT and departements of our University. The third part includes the continuation of
the project on telepathology by establishing a pilot study on telediagnosis on intraoperative frozen
sections. This pilot includes also a quality and feasibility study. The telpathoogy link is proposed
between a county hospital (Ogulin) and the University departement. POTENTIAL BENEFITS: of these
studies is a more precise diagnosis or prognosis of malignant tumors enabling a more individualised
therapeutic approach. The experimental part is supposed to yield some new therapeutic drugs and
intraoperative telediagnosis will certanly increase the quality of treatment in the respective hospital.
In this sence the educational potential of tlepathology shuld not be neglected.
Project Website
www.mzos.hr
Contact Person
Name
MD, PhD Sven Seiwerth
Email
[email protected]
Function
Professor
Phone
+38514566977
Fax
+38514921151
Pathology
Website
www.mef.hr
Research Team Objectives
Main Fields
Pathology
Pharmacology
Quantitative analysis in tumor and experimental pathology. Prognostic factors in tumor pathology.
Angiogenesis. Wound healing. Telepathology.
Partners/Interests
researcher
industrial partner
Pathology
Pharmacology
Information Technology, Statistics, Documentation
Should have knowledge in molecular biology and its methods, applicable in molecular pathology. New
technology in microscopy for testing.
Continuing research in the field of tumors, (especially prognostic factors and tumor heterogeneity)
wound healing and angiogenesis.
Institution/University
Name of Institution/University
University of Zagreb Medical School , Department of Pathology
Number of Employees
45
Number of Researchers
13
Country
Croatia
Postal Adress
Salata 10
10000 Zagreb
Pathology
Research Team
Research Team Name
Translating histogenetic and molecular variability of CD117
in human tumors to the innovative targeted therapies
Individual Researcher
No
Research Team Leader Name
Prof. MD Carmen Ardeleanu
Contact Person
Name
Prof. MD Carmen Ardeleanu
Email
[email protected]
Function
Head of the Pathology Department
Phone
+40 21 3192734
Fax
+40 21 3192734
Website
Research Team Objectives
Main Fields
Pathology
Translating research for human health CD117, genomics, human tumors, targeted therapy
Partners/Interests
researcher
Pathology
Objectives: To improve a targeted antitumor therapy by identifying histogenetic and molecular
variability of CD117 in human tumors. The expected results: A better characterization of human
tumors with alterations in CD117, by means of molecular analysis aiming optimal targeted therapy.
The impact of the project: Optimal and efficient treatment of CD117 defective tumors based on
molecular selection. Contribution to the EU or regional policy: Updating knowledges about the signal
transduction pathways in human tumors and upgrading a molecular diagnosis and targeted therapy,
with impact on public health. European research potential: The project offers an opportunity for
cancer oriented translational research in a developing field of targeted therapy with real social and
economic benefits. The research base offered by the project would furnish successful performances in
translating results in practical medicine. European added value: The project contributes for enhancing
visibility of European research excellence and translating research results for human health.
Institution/University
Name of Institution/University
"Victor Babes" National Institute of Pathology
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Romania
Postal Adress
Spl. Independentei 99-101
50096 Bucharest
Pathology
Research Team
Research Team Name
University Department of Pathology "Ljudevit Jurak"
Individual Researcher
No
Research Team Leader Name
MD Alma Dubravic
Research Team Members
Team Member 0
MD Ante Reljic [[email protected]]
Team Member 1
B.Sc. Zlatko Papes [[email protected]]
Team Member 2
MD Ivana Nola [[email protected]]
Team Member 3
MD Zvonko Kejla [[email protected]]
Team Member 4
B.Sc. Zdenka Silek-Saran [[email protected]]
Team Member 5
MD Jaksa Filipovic-Cugura [[email protected]]
Team Member 6
MD Tomislav Plasaj [[email protected]]
Team Member 7
MD Josip Fajdic [[email protected]]
Team Member 8
MD Jasna Talan-Hranilovic [[email protected]]
Team Member 8
MD Ana Krvavica [[email protected]]
Team Member 10
MD Monika Ulamec [[email protected]]
Team Member 11
MD Marina Kos [[email protected]]
Team Member 12
MD Drinko Balicevic [[email protected]]
Team Member 13
MD Majda Vucic [[email protected]]
Team Member 14
MD Davor Tomas [[email protected]]
Team Member 15
MD Hrvoje Cupic [[email protected]]
Team Member 16
MD Dora Brezovecki-Bidin [[email protected]]
Team Member 17
MD Alma Dubravic [[email protected]]
Team Member 18
MD Bozo Kruslin [[email protected]]
Team Member 19
MD Mladen Belicza [[email protected]]
Team Member 20
MD Marija Strnad [[email protected]]
Research Team Projects
Project Name
Organization and evaluation of pathoanatomical tumour
registry and tumour bank
Project Leader
MD Mladen Belicza [[email protected]]
Project Funding Agency
Ministry of Science, Education and Sports
Project Budget
4055 €
Project Start Date
0000-00-00
Project End Date
0000-00-00
Project Partners
Project Summary
Registries of different diseases, including tumors, play an impontant role in the epidemiology, followup of patients and evaluation of therapy, and represent a basis for scientific research. Evaluation of
such registries showed many failures like inappropriate coding of diagnosis and tumor side and type
leading to misinterpretation of data on the incidence, histologic type and behaviour of tumors.
Technological advances enable investigation of the genome offering new perspectives for diagnosis
and treatment. Such investigationes largely depend on adequacy of the material stored in tumor
tissue banks. Pathologists with their expertise and responsibility for histopathologic diagnosis play a
central role in collection of human tissues, in accordance with medical, legal and ethical standards,
not only for diagnostic purposes, but also for research. The aim of this project is development and
evaluation of the computer tumor registry of biopsy and autopsy data at the Deprtment of Pathology.
Registry will contain crucial clinical and laboratory findings representing the source of data that could
be used by National Registry of Cancer. We will analyse the incidence of tumors. The nomenclature,
classification and coding of diagnoses will be performed according to the International Classification of
the Diseases (MKB 10). In routine work we will use standard histologic, histochemical and
Pathology
immunohistochermical methods, and in situ hybridisation. Tissue samples embedded in paraffin will
be stored during at least 10 years period representing the basis of tumor tissue bank. Certain tumors
will be stored during longer period in deep freezer in liquid nitrogen. Computer-based data coded
according to the MKB 10 will enable us to follow cancer patients and exchange data with other
republic centres and other countries. Tissue tumor bank will provide a permanent source of samples
for additional biomedical resarch.
Project Website
Contact Person
Name
MD Alma Dubravic
Email
[email protected]
Function
Research Assistant
Phone
+385 1 37 87 465
Fax
+385 1 37 87 244
Website
www.kbsm.hr/Jurak/
Research Team Objectives
Main Fields
Pathology
Epidemiology
Information Technology, Statistics, Documentation
Development and evaluation of the computer tumor registry and autopsy data. Analysis of the
incidence of some tumors, particulary of the breast, genitourinary, gastrointestinal, and central
nervous system, thyroid gland, melanomas, and incidental tumors discovered at autopsy.Creating of
tumor tissue bank.
Partners/Interests
researcher
industrial partner
Pathology
Genetics
Surgery
Interests in improving computer-based data project, ideas on use of tumor tissue bank. Interest in
scientific researching of tumor blood vessels, and similar topics conected to tumors behaviour.
Research of changes in blood vessels that supply tumors, and their impact on tumor behaviour
Institution/University
Name of Institution/University
University Department of Pathology "Ljudevit Jurak"
Number of Employees
36
Number of Researchers
15
Country
Croatia
Postal Adress
Vinogradska cesta 29
10000 Zagreb
Pediatrics
Research Team
Research Team Name
Department of Paediatrics
Individual Researcher
No
Research Team Leader Name
Mr. MD, PhD Alojz Gregoric
Contact Person
Name
Mr. MD, PhD Alojz Gregoric
Email
[email protected]
Function
Professor of Paediatrics
Phone
+386 2 321 2416
Fax
+686 2 331 2393
Website
www.sb-mb.si
Research Team Objectives
Main Fields
Pediatrics
Partners/Interests
researcher
Suitable
Paediatrics
ANALYSIS OF CANDIDATE GENES FOR VESICO - URETERIC REFLUX Introduction. Primary vesicoureteric reflux (VUR) is a common urological anomaly in children with an approximate incidence of
1% in newborns. It is a disease of genetic origin and it shows a strong familial association. The VUR
appears to be inherited in an autosomal dominant mode with reduced penetrance but candidate
genes have not been described yet. Data from a genome wide linkage study and from studies on
knock-out mice with VUR phenotype suggest that several other genes if mutated might cause the
development of VUR independently. Such candidate genes causing VUR in mice are genes of
angiotensin II receptor type 2 (AGTR2) and uroplakin 3 (UPK3). In addition, variable clinical
presentations of the disease in affected families suggest that other genes might affect the penetrace
of VUR. Single nucleotide polymorphisms such as C825T in the gene for G protein ©¬3 subunit
(GNB3), L10P in the gene for transforming growt factor ©¬1 (TGFB1) and C2046T in the gene for the
¥á1 chain of collagen type 1 (COL1A1) have biological consequences and they could influence the
development of VUR. Methods. DNA was extracted from venous blood from 85 patients diagnosed
with VUR. Coding exons of genes AGTR2, UPK3 and UPK1B were screened using heteroduplex
analysis and detected nucleotide variations were confirmed with sequencing. Polymorphisms C825T,
L10P and C2046T were detected with the PCR-RFLP method. Results. A sequence variation changing
asparagin to aspartic acid at the codon 137 (N137D) in exon 5 of the UPK1B gene was detected in
4.7% of patients with VUR and in 0.83% of controls (OR = 5.87, 95%Cl 1.54-22.33, p = 0. 017). Also
an overrepresantation of 825T allele in the GNB3 gene was observed in patients when compared to
the control group of healthy individuals (¥ö©÷ = 7.38, p = 0.025, df = 2). Conclusions. Our results
implicated UPK1B gene in the development of VUR and the detected aminoacid substitution N137D
might be a direct cause for the disease. Also the C825T polymorphism from the GNB3 gene might be
involved in the development of the VUR, possibly as a genetic modifier. Genes AGTR2 and UPK3 as
well as polymorphisms L10P from gene TGFB1 and C2046T from gene COL1A1 were not associated
with the development of VUR in our sample of patients. At the moment we are collaborating in this
research project with Medical Faculty Rijeka and Split, but we are interested to spread our research to
other Medical Faculties of Medical Research Initiative South Eastern Europe. The aim of the study is to
analise 300 - 500 children for candidate genes for VUR.
Institution/University
Name of Institution/University
General Hospital Maribor
Number of Employees
2400
Number of Researchers
33
Country
Slovenia
Postal Adress
Ljubljanska 5
2000 Maribor
Pediatrics
Research Team
Research Team Name
Kalmanti Maria Stiakaki Eftichia Martimianaki Georgia
Choumerianou Despina
Individual Researcher
No
Research Team Leader Name
Dr Helen Dimitriou
Research Team Projects
Project Name
Adult mesenchymal stem cells engineering for connective
tissue disorders. From the bench to the bed side.
Project Leader
Prof Christian Jorgensen [[email protected]]
Project Funding Agency
European Union
Project Budget
4500000 €
Project Start Date
2003-01-01
Project End Date
2007-12-31
Project Partners
Project Website
www. genostem.org
Contact Person
Name
Dr Helen Dimitriou
Email
[email protected]
Function
Assistant Professor
Phone
+32810 394674 or +32810 394664
Fax
+32810 394673
Website
http// med uoc.gr
Research Team Objectives
Main Fields
Paediatrics
Biology
Genetics
Hemopoiesis in childhood malignancies · Tissue Culture and Stem cell assays · Bone Marrow Purging
by PDT (Photodynamic treatment) · Evaluation of cord blood as a source of hematopoietic progenitors
for transplantation, · Late effects of chemotherapy · Phase II/III clinical studies in pediatric
malignancies · Stem cells from different sources (BM, PB, CB). Isolation, characterization and ex vivo
expansion · Isolation, characterization, ex vivo expansion and in vitro differentiation of bone marrow
mesenchymal stem cells · Oncogene expression and transformation potential of stem cells · Cell cycle
study and immunophenotype of stem cells. · Study of apoptosis of stem cells.
Partners/Interests
researcher
industrial partner
Paediatrics
Genetics
Biology
Experience in cell biology, molecular techniques, tissue culture techniques, animal model facilities
Ex vivo expansionmolecular characterization differentiation, transformation potential of stem cells
Hemopoiesis in childhood hematological disorders Effect of external stimuli on the apoptotic features
of cells derived from bone marrow of children Late effects of chemotherapy on children treated for
malignancies Photodynamic treatment for bone marrow purging
Institution/University
Name of Institution/University
University of Crete , Medical School
Number of Employees
800
Number of Researchers
600
Country
Greece
Postal Adress
PO Box 2208 0
GR 71 Heraklion, Crete
Pediatrics
Research Team
Research Team Name
South-East Balkan Endemic Nephropathy Group (SEBENG)
Individual Researcher
No
Research Team Leader Name
Professor of pediatrics Amira Peco-Antic
Research Team Members
Team Member 0
MD, PhD Danica Bukvic [[email protected] ]
Team Member 1
Marina Subasic []
Team Member 2
Assistent Danka Pokrajac-Milincic []
Team Member 3
Associated professor Velibor Tasic []
Team Member 4
Professor Marusia Lilova []
Team Member 5
Professor Jasmina Markovic-Lipkovski []
Team Member 6
MD Jelena Maletkovic [[email protected]]
Team Member 7
PhD Ana Gligic [[email protected]]
Team Member 8
MD Dusan Paripovic [[email protected]]
Team Member 9
Professor Amira Peco-Antic [[email protected]]
Contact Person
Name
Professor of pediatrics Amira Peco-Antic
Email
amir[email protected]
Function
Head of Nephrology department, University children
Phone
381 11 2772 591
Fax
381 11 3612858
Website
www.udk.bg.ac.yu/klinika/
Research Team Objectives
Main Fields
Paediatrics
Public health services
Genetics
Progression and prevention of chronic kidney disease in children, hemmorhagic fever with renal
involvment and Balkan endemic nephropathy.
Partners/Interests
researcher
Genetics
Clinical microbiology
Paediatrics
Pediatric and adult nephrologist who is involved in the medical care of patients suffering from Balkan
endemic nephropathy and/or hemmorhagic fever with renal involvment and their pediatric relatives.
Many chronic kidney diseases (CKD) which may lead to terminal renal failure in adults have beginning
in childhood. Predictors of these diseases are only partially known and traditional risk factors such as
hypertension, proteinuria and decreased glomerular filtration rate are in fact, the indicators of
established renal damage. Therefore, the search for more sensitive risk markers continues. The
present study is a prospective cohort study that will employ an extensive array of risk factors
(epidemiological/environmental, genetic and clinical) that can explain genesis of Balkan endemic
nephropathy (BEN). Epidemiological and environmental investigations will include isolation and
characterisation of hantaviruses from the positive animals in endemic regions of BEN (rodent’s lung,
antipodes and other wild and domestic animals) as well as in material (urine, blood) from the BEN
patients and from the patients with hemorrhagic fever with renal involvement. Immune status on
hantaviruses will be investigated in the BEN patients and in their healthy pediatric relatives. Genetic
analysis will be focused on genes RET, GDNF and GFRA-1 which have the critical role in renal
development as demonstrated in knockout mouse models and on the polymorphisms of the reninangiotensin system genes and polymorphisms of the most important detoxicating enzymes genes that
may have a role in BEN development. Clinical part of this study will be concentrated on assessment of
sensitive markers of CKD in the pediatric relatives of BEN patients. Therefore we will examine
glomerular hyperfiltration that is a crucial event for progression of CKD. It will be estimated from the
following: a renal functional reserve (RFR) that will be detected by measurement of the change in
creatinine clearance following a meat-free protein meal in children pre-treated with cimetidin,
Pediatrics
quantitative and qualitative analyses of proteinuria (global, albumin, immunoglobulin G and
SDS/PAGE) pre and after the test of physical exercise, and the maximal bipolar renal measurement
done by real-time ultrasound scanning. To evaluate which antedates, loss of RFR and renal
dysfunction or nondipper type of circadian rhythm of blood pressure (BP) the following examinations
will be done: nocturnal and diurnal BP , nocturnal and diurnal natriuresis and proteinuria and the
relationship of GFR and RFR with night/day rations of BP, urinary sodium and protein excretions. We
hope that our investigation will show that genetic and epidemiological/environmental factors (viruses)
have a role in development of BEN. If we prove that early signs of disease may be found in children
and adolescents from endemic regions it will be very important for early start of renal protection.
Institution/University
Name of Institution/University
University children's Hospital
Number of Employees
150
Number of Researchers
10
Country
Serbia and Montenegro
Postal Adress
Tirsova 10
11000 Belgrade
Pediatrics
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Prof Senka Mesihović-Dinarević MD FESC
Contact Person
Name
Prof Senka Mesihović-Dinarević MD FESC
Email
[email protected]
Function
Head of the Department of Paediatrics
Phone
+33 663 742 / 743
Fax
+33 203 670
Website
www.mf.unsa.ba
Research Team Objectives
Main Fields
Paediatrics
Partners/Interests
RISK FACTORS FOR THE DEVELOPMENT OF ARTERIOSCLEROSIS IN PAEDIATRIC
POPULATION
Lipids are recognized as a significant risk factor in the development of atherosclerotic processes as
well as hypertension. The appearance of clinical manifestations of atherosclerosis comes in
unpredictable periods of life. The investigations which show the initial phase are mostly limited by the
small number of nosological groups in different populations, so we aim to start the investigations on
this topic in the South-East region of Europe. In childhood it is possible to study the pathological
precursors of cardiovascular disease and risk factors which accelerate the development of the
atherosclerotic process. The basic research of lipoproteins, apolipoprotein metabolisms, the biology of
developing atheroma processes in the cell and the role of genetics in creating coronary artery disease
are necessary in the field of preventive cardiology.
The aim of this project would be to investigate the levels of serum lipoproteins and blood pressure in
the paediatric population age 0 do 18 years with suspicion of cardiovascular disease, to evaluate the
correlation between cardiovascular disease other than atherosclerosis and the appearance of
dyslipoproteinaemia in this population.
Patients and methods: the investigations should be carried out at University clinical Centre Sarajevo,
Paediatric clinic, paediatric cardiology department and out patient’s clinic, during the period from 3
years as well as in the other European Paediatric clinic in the region mentioned above. It would
include approximately 300 patients, age 0-18 years with control group. The criteria of the European
Community would be used during random choice of patients and the conditions for including or
excluding patients. Age groups should be formed as: I (0.30 days), II (1-12 months), III (1-4 years),
IV (4-7 years), V ( 7-11 years), VI (11-14 years) and VII (14-18 years). The control group would be
formed from healthy children in relation to age forming 4 subgroups: I (4-7 years), II (7-11 years),
III (11-14 years) and IV (14-18 years). The patients who would be included should satisfy the
following conditions: undisturbed carbohydrates metabolism, orderly functions of thyroid, kidneys and
liver with suspicion of cardiovascular disease. The excluding criteria would be: presence of diabetes
mellitus, hypothyroids, acute disease and reconvaleascency. The parameters in the investigations are
planned to be objective: age, sex, height, weight, blood pressures measurements (including 24 -72
hrs monitoring), pulse, saturation of oxygen, ESR, FBC, urine, glucose level in blood, urine and
creatinin levels, liver enzymes, alkali and acid phosphates, status of lipoproteins (total cholesterol,
triglycerides, LDL cholesterol, LDH cholesterol, apoplipoproteins, ECG; EFCG, echocardiography; and
subjective: history (personal and family), smoking, alcohol in the family, sport activities, practical
works and length time spending in school, type of nutritional habits and calories of intake meals.
Since nutritional intake could affect the status of lipoproteins in serum (during pregnancy, brest
feeding input) so nutritional status of each patients should be noted.
The expectations of this project are: to discover the pathological precursors of cardiovascular disease
and risk factors which could influence the development of the atherosclerotic process in childhood
which represents a wide spectrum of medical investigation, with the aim of delaying the development
of atherosclerotic vascular lesions in the adult population.
Pediatrics
Institution/University
Name of Institution/University
University of Sarajevo, Faculty of Medicine Sarajevo
Number of Employees
Number of Researchers
Country
Bosnia and Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Pharmacology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
MD, PhD, MA Slobodan Jankovic
Research Team Projects
Project Name
Pharmacological analysis of effects of biologically active
substances on smooth muscles from gastrointestinal and
urogenital tract.
Project Leader
Professor Slobodan Jankovic [[email protected]]
Project Funding Agency
Ministry of Science of Republic of Serbia
Project Budget
50.000 €
Project Start Date
2006-01-01
Project End Date
2011-12-31
Project Partners
Project Summary
Investigation of effects and mechanism of action of glutamate on isolated human ureter and rat
colon. The effect is being tried on both spontaneous and electric field-stimulated contractions. Also,
effects of antiepileptics on isolated Fallopian tubes are investigated.
Project Website
Contact Person
Name
MD, PhD, MA Slobodan Jankovic
Email
[email protected]
Function
Professor of pharmacology
Phone
+381641168103
Fax
+38134370073
Website
medf.kg.ac.yu
Research Team Objectives
Main Fields
Pharmacology
Therapeutics
Effects of biologically active substances on tone and spontaneous contractions of isolated smooth
muscle preparations, of both human and animal origine. Special interest in smooth muscles from
human ureters, Fallopian tubes and gastrointestinal tract. Among the substancies studied are
glutamate, antiepileptics, derivatives of cisplatin. The other fields of research are
pharmacoepidemiology, pharmacovigilance and pharmacoeconomy.
Partners/Interests
researcher
Pharmacology
Pharmacologist or physiologist
To investigate effects of antituberculotics on isolated human ureter and Fallopian tubes.
Institution/University
Name of Institution/University
Medical Faculty, University of Kragujevac
Number of Employees
210
Number of Researchers
50
Country
Serbia and Montenegro
Postal Adress
Svetozara Markovica 69
34000 Kragujevac
Pharmacology
Research Team
Research Team Name
Department of Pharmacology / Medical Faculty of the
University of Split / Croatia
Individual Researcher
No
Research Team Leader Name
Professor Mladen Boban
Research Team Members
Team Member 0
Professor Mladen Boban [[email protected]]
Team Member 1
M.D. Jonatan Vukovic [[email protected]]
Team Member 2
M.D. Ivana Mudnic (Music) [[email protected]]
Team Member 3
M.S., M.D. Darko Modun [[email protected]]
Team Member 4
M.D. Ivica Brizic [[email protected] ]
Research Team Projects
Project Name
Extracorporeal heart preservation
Project Leader
Professor Mladen Boban [[email protected]]
Project Funding Agency
Ministry of Science, Republic of Croatia
Project Budget
33000 €
Project Start Date
2002-09-01
Project End Date
2006-06-01
Project Partners
Slovenia / University of Ljubljana Faculty of Medicine / Institute of
Pharmacology and Experimental Toxicology / Laboratory of
Cardiovascular Research / Gorazd Drevensek (head)
Project Summary
The development of methods for prolonged preservation of donor hearts is one of the most important
problems of cardiac transplantation. Cardioplegic arrest and hypothermic storage, the most common
method of donor heart preservation during transport, does not provide adequate cardiac protection
longer than few hours. If hearts could be preserved for at least 20 hours the pool of donor hearts
would be increased, as would electivity of the operation, potential for tissue typing, and anonymity of
donors. Numerous pharmacological interventions on improving extracorporeal heart preservation are
directed toward: reduction of heart metabolic needs during isolation, cellular swelling, application of
precursors for energy - rich intermediates, free radical scavengers, and coronary flow protectors. On
the other side, it has been shown that moderate wine consumption, particularly red wine, provides
beneficial effects on cardiovascular system and human health. It seems that antioxidants from the
polyphenolic wine fraction play crucial role in that process. Some compounds from that group
(catechin, quercetin, resveratrol) have been effective in protecting hearts after ischemia and hypoxia,
inhibiting platelets aggregation, enhancing nitric oxide production, preventing low density lipoproteins
oxidation… Hence, we will investigate protective effects of the standardized wine extract applied in
vitro and as a pre-treatment in vivo, on heart and coronaries subjected to short and long - term
hypothermic isolation. In order to achieve synergistic protective effects, the wine extract will be also
combined with butanedione monoxime, a negative inotropic agent, which significant protective effects
we have proved in these models. Protective effects on the isolated heart will be manifested as a
better improvement of the contractility, coronary flow, oxygen consumption usefulness,
electrophysiological stability, decreased biochemical markers of oxidative stress (lipid peroxides) and
tissue injury (creatine kinase, lactate dehydrogenase and troponin), histological preservation and
enhanced nitric oxide production. Wine extracts protective effects on the isolated coronary rings will
be presented as stronger vasodilatation responses to endothelium dependent (acetilcholine) and
independent (nitroprusside, pinacidil) vasodilators. This research is of relevance for many other
conditions and procedures in cardiology and cardiosurgery, and is also stimulative for healthier
feeding habits.
Project Website
Contact Person
Name
Professor Mladen Boban
Email
[email protected]
Function
Head of Department
Phone
+38521557904
Pharmacology
Fax
+38521465073
Website
www.mefst.hr
Research Team Objectives
Main Fields
Pharmacology
Clinical chemistry
Physiology
Red wine, cardiovascular system, oxidative stress, polyphenolics, uric acid.
Partners/Interests
researcher
industrial partner
Pharmacology
Clinical chemistry
Physiology
We are interested in research partners who are familiar with analytical methods for human biological
samples, like LC MS analysis of plasma. Also we are looking forward to adopt new methods for
evaluation of vascular parameters in humans, especially focusing on estimation of endothelial
function. We are interested in industrial partners who will find their interest in this detailed study on
mechanisms of bilogocal effects of wine and wine-derivate products. There is a great potential for
production of new, improved, and biologically more potent wine products.
Numerous studies have shown that moderate consumption of wine, especially red wine, has beneficial
effects on cardiovascular system. These effects are mainly attributed to wine polyphenolics.
Polyphenolic wine extracts and some polyphenolic compounds (resveratrol, catechin e.g.) are among
others, associated with reduced susceptibility of LDL to oxidation, reduced platelets aggregation,
increased production of nitric oxide (NO) and increase of human plasma antioxidant capacity. Recent
studies have shown that polyphenolics are poorly absorbed and that their plasma concentrations are
too low to explain some biological effects of wine. For instance, an elevation of plasma antioxidant
capacity after red wine intake is significantly related to the acute elevation of plasma urates.
Phenomenon of that urate elevation is polyphenolics-independent and its biological relevance is
unknown. With this study, we want to separate the effects of wine polyphenolics from the effects of
other wine constituents. Our aim is to determine in what extent the effects observed in in vitro
biochemical measurements and isolated organ studies can be linked with the effects in humans after
intake of tested wines and beverages based on that wines.
Institution/University
Name of Institution/University
Medical Faculty of the University of Split
Number of Employees
125
Number of Researchers
80
Country
Croatia
Postal Adress
Soltanska 2
21000 Split
Pharmacology
Research Team
Research Team Name
Drug delivery group (formulation and delivery of
conventional drug molecules and products of biotechnology)
Individual Researcher
No
Research Team Leader Name
professor Katerina Goracinova
Research Team Members
Team Member 0
professor katerina goracinova
[[email protected]]
Team Member 1
assistant professor renata raicki [[email protected]]
Team Member 2
assistant to prof marija glavas [[email protected]]
Team Member 3
PhD student maja simonoska [[email protected]]
Team Member 4
PhD student nikola geskovski [[email protected]]
Research Team Projects
Project Name
INFLUENCE OF BIOPOLYMER INTERACTIONS ON THE DRUG
DELIVERY FROM CHITOSAN-ALGINATE COLLOIDAL CARRIER
SYSTEMS
Project Leader
professor Katerina Goracinova (pharmacy group)
[[email protected]]
Project Funding Agency
NATO Science for peace program
Project Budget
150000 €
Project Start Date
0000-00-00
Project End Date
0000-00-00
Project Partners
Brian Amsden, Queen's University, Kingston, ON, Canada - NATO
Country Project Director Macedonia Aleksandar Andonovski,
Institute of Physics, Faculty of Natural Sciences and Mathematics,
SS. Cyril and Methodious University, Skopje, Republic of Macedonia
- Partner Country Project Director Physics group) Katerina
Goracinova, Faculty of Pharmacy, SS. Cyril and Methodious
University, Skopje, Republic of Macedonia - Partner Country Project
Co-Director / Pharmacy group
Project Summary
Implementation of the knowledge in the development of the drug targeted biopolymer
microparticulate carrier system was done, by which the improvement of the delivery and efficacy of
incorporated drug and decreasing of adverse effects and toxicity was achieved. The technological
process on small laboratory and larger-scale production of the well-defined biopolymer microparticles
will be also established. The product designed is the mucoadhesive microparticulate polysaccharide
drug carrier and polysaccharide based microspheres coated with enterosoluble material
Project Website
http://www.cs.org.mk/web/index.htm
Project Name
PREPARATION AND EVALUATION OF MICROPARTICLES FOR
ORAL CONTROLLED DRUG DELIVERY
Project Leader
professor katerina goracinova
[[email protected]]
Project Funding Agency
TUBITAK and Macedonian Ministry of science
Project Budget
15000 €
Project Start Date
2005-03-02
Project End Date
2008-03-02
Project Partners
Professor Atila Hinchal, University of Haccettepe, Ankara, Turkey
Professor Sema Calis/ University of Hacceteppe, Ankara, Turkey
(co-director from Turkish side)
Project Summary
Intravenous administration of most drugs for colon cancer therapy produces severe systemic side
effects due to their cytotoxic effect on normal cells. Development of site specific oral formulations for
controlled colon-specific delivery would be a novel approach for increasing the drug efficacy and
Pharmacology
lowering the drug side effects. Also lectin mediated micro and nano particulate specific delivery can
combine two functions in anticancer drug delivery systems, being targets to cancer cells and inducing
anticancer effect because in tumor pathology aberrant glycosilation is a common attribute of
neoplastic growth and lectins which selectively and reversibly bind to carbohydrate structures have
demonstrated the ability to induce or control a number of metabolic and proliferative processes.
Preparation, characterization of bioefficient highly specific controlled release DDS for delivery of
different anticancer model substances to the colon will lead to efficient targeted anticancer therapy
with lowering of toxic and side effects of the anticancer drugs.
Project Website
Project Name
IMMOBILIZATION OF BIOACTIVE SUBSTANCES IN
BIODEGRADABLE HYDROGELS OR FUNCTIONALIZED
MICELLS; POSSIBLE APPLICATION AS BIOCATALYSTS OR
DRUG RELEASE SYSTEMS
Project Leader
professor maja cvetkovska [[email protected]]
Project Funding Agency
Mac ministry of Science and BAN
Project Budget
15000 €
Project Start Date
0000-00-00
Project End Date
0000-00-00
Project Partners
professor Hristo Cvetanov, Bulgarian Academy of Science professor
Katerina Goracinova, Faculty of Pharmacy
Project Summary
incorporation of drug molecules in polymeric microparticulate systems for improving delivery and
efficacy
Project Website
Contact Person
Name
professor Katerina Goracinova
Email
[email protected]
Function
head of the institute of pharm techn and biopharmacy
Phone
+38923126024
Fax
+38923123054
Website
www.cs.org.mk/web/index.htm ; www.ukim.edu.mk
Research Team Objectives
Main Fields
Pharmacy
Therapeutics
Other allied sciences
Research team main fields are within the scope of formulation science, dosage form design and
evaluation, targeted delivery, mucoadhesive drug delivery, absorption enhancement. The main areas
of drug delivery problem solving are: 1. Formulation ofn Controlled and Targeted drug delivery
systems 2. Application of New Biomaterials as colloidal drug carriers- microspheres, nanospheres,
liposomes 3. Alternative routes of application of peptides and proteins and their optimal stability and
activity 4. Evaluation of Drug/polymer, protein/polymer interactions The group mission is scientific
research in drug delivery with emphasis on efficaceous treatment (targeted and controlled delivery)/
and or prevention of diseases
Partners/Interests
researcher
industrial partner
research partner
Pharmacy
drug delivery of conventional drug molecules and products of biotechnology
Institution/University
Name of Institution/University
Faculty of Pharmacy; Univ Sts Cyril and Methody
Number of Employees
40
Number of Researchers
20
Country
Macedonia
Pharmacology
Postal Adress
Vodnjanska 17
1000 Skopje
Pharmacology
Research Team
Research Team Name
Pharmacology
Individual Researcher
No
Research Team Leader Name
Mrs., PhD Irena Mlinarić-Rašćan
Contact Person
Name
Mrs., PhD Irena Mlinariè-Rašèan
Email
[email protected]
Function
Asist. Professor of Pharmacology
Phone
+ 386 1 47 69 645
Fax
+386 (0)1 425 80 31
Website
http://www.ffa.uni-lj.si/index.php/eng/content/view/full/43
Research Team Objectives
Main Fields
Pharmacology
Partners/Interests
researcher
industrial partner
Suitable
Pharmacology
1. INNOVATIVE DRUGS –TARGETING MOLECULAR BASIS OF CELL DEATH.APOPTOSIS Serine
proteases have been shown to actively participate in the process of apoptosis in mammalian cells.
The oustanding example is Bortezomib (Velcade®), a reversible inhibitor of the chymotrypsin-like
activity of the proteasome that has been recently approved for treatment of multiple myeloma. Our
previous studies revealed two distinct roles for serine proteases in apoptotic process. Firstly, we have
shown that inhibition of anti-apoptotic serine proteases governs the onset of the caspase-dependant
apoptotic cascade. Secondly, we have also demonstrated the involvement of a serine protease in the
terminal stage of apoptosis by showing that chymotrypsin–like protease activity is required for
internucleosomal DNA fragmentation in apoptotic cells. The overall goal of the proposed study is to
further elucidate the role of serine proteases in molecular mechanisms underlying apoptotic cell
demise, particularly in oligonucleosomal DNA cleavage. We aimed to address these issues by two
parallel strategies. Firstly, we intend to use a direct pharmacogenomic approach to screen newly
synthesized serine protease inhibitors for the potency to induce apoptosis in the absence of
internucleosomal DNA laddering. We are further to use newly-identified inhibitor as a bait to isolate
the interacting molecule, which followed by N-terminal sequencing and cloning of responsible gene
shall lead to the identification of a key molecule. Simultaneously, a reverse pharmacogenomic
approach shall be applied in which selected recombinant serine proteases will be tested for inhibition
with selected inhibitors. Both approaches shall converge at the stage of identification of a serine
protease whose inhibition leads to the desired phenotype, namely to induce caspase dependent
apoptosis and/or interfere with DNA oligonucleosomal laddering. Further functional studies shall
include over-expression and down-regulation of the newly identified gene, in order to prove the
hypothesis. 2. PHARMACOGENTICS: TIOPURINE PHARMACOGENETICS from bench to bedside
Thiopurine pharmacogenomics is the study of how an individual’s genetic inheritance affects the
body’s responses to drugs. The application of pharmacogenomics in the individualized drug therapy
offers the potential to improve drug effectiveness, reduce adverse side effects, and provide costeffective pharmaceutical care. Objective of proposed project encompasses from bench to bedside
concept, emphasizing the need of basic research application in the current clinical practice. The
project will be conducted by interdisciplinary team including a pediatric oncologist, molecular
geneticists and a clinical biochemist and will provide novel concepts for application in the clinic as well
as the importance of linking the first-hand clinical experiences to problem-solving oriented research.
We aim to obtain three levels of knowledge and implement them in daily clinical practice: (1) Genetic
characterization of the patient’s drug profile. Application of the diagnostics based TPMT
polymorphisms and HPLC enzyme activity analysis, developed in our laboratory. (2) Establishment of
novel diagnostic tests by the identification and characterization of other candidate genes, or by using
a genome scale non-candidate approach. (3) Understanding of the molecular mode of action of
thiopurines and the metabolism of key enzymes. Proposed study will contribute to the further
development of pharmacogenomics, a field of study that combines the knowledge emerging from the
Human genome project with classical pharmaceutical disciplines. By implementing the proposed
project we also contribute to common European research area and mobility of researchers.
Pharmacology
Institution/University
Name of Institution/University
Faculty of pharmacy
Number of Employees
113
Number of Researchers
60
Country
Slovenia
Postal Adress
Askerceva 7
1000 Ljubljana
Pharmacology
Research Team
Research Team Name
Pharmacology
Individual Researcher
No
Research Team Leader Name
Professor Milica Prostran
Research Team Members
Team Member 0
Professor Marina Stojanov [[email protected]]
Team Member 1
Associate Professor Branislava Miljkovic
[[email protected]]
Team Member 2
Research Associate Zorica Nesic [[email protected]]
Team Member 3
Research Associate Nevena Divac [[email protected]]
Team Member 4
Research Associate Radan Stojanovic [[email protected]]
Team Member 5
Associate Professor Sonja Vuckovic [[email protected]]
Team Member 6
Associate Professor Zoran Todorovic [[email protected]]
Team Member 7
Professor Milena Pokrajac [[email protected]]
Team Member 8
Professor Ranka Samardzic [[email protected]]
Team Member 9
Professor Milica Prostran [[email protected]]
Team Member 10
Research Assistant Katarina Vucicevic
[[email protected]]
Team Member 11
Senior Res. Fellow Eleonora Dzoljic [[email protected]]
Team Member 12
Research Assistant Marija Petronijevic
[[email protected]]
Contact Person
Name
Professor Milica Prostran
Email
[email protected]
Function
Chair of Postgraduate Study in Pharmacology
Phone
+381-11-2684363
Fax
+381-11-2686025
Website
med.bg.ac.yu
Research Team Objectives
Main Fields
Pharmacology
Internal Medicine
Neurology
Our investigation deals with the modulation of action of inflammatory mediators (e.g. NO, ROS etc.)
in experimental models (e.g. ischemia-reperfusion injury, pain or muscle contractility), as well as in
clinical settings (e.g. ARF or ACS), including the pharmacokinetics of drugs with antiinflammatory
actions.
Partners/Interests
researcher
Pharmacology
Internal Medicine
Neurology
- Basic Medicine reseachers (pathology and biochemistry of inflammation) - Clinicians (neurology,
cardiology, nephrology)
Our project will deal with the modulation of action of inflammatory mediators (e.g. NO, ROS etc.) in
three different experimental models: a) ischemia-reperfusion injury; b) pain; and c) muscle
contractility a) Renal ischemia and reperfusion (I/R) may cause both apoptotic and necrotic renal cell
death leading to acute renal failure (ARF). Pharmacological interventions against ARF, successful in
animal models, have proven to be largely negative in clinical conditions and did not decrease a high
mortality in such patients. Novel therapeutic approach to ARF should involve modulation of
proinflammatory network of events that lead to apoptosis and/or necrosis of kidney cells via
activation of NFkappaB. Acute pretreatment with statins (e.g. simvastatin, pravastatin, atorvastatin
etc.) have protected myocardium and/or kidney in certain models of I/R injury, the mechanism(s) of
such action remaining to be established. Rat I/R injury in vivo model would be used for the
Pharmacology
investigation of the acute protective effects of statins, and the following parameters would be
analyzed: MAP, HR, histopathological score including immunohistochemistry, and selected
biochemical parameters (serum and urine electrolytes, transaminases, and creatinine clearance, i.e.
parameters of glomerular and tubular function). The model used was established in collaboration with
the WHRI, London, UK (e.g. Chatterjee et al. 2004; 2005). b) Evaluation of the antinociceptive and
antiinfammatory effects, as well as effects on the body temperature and other behavioural effects
(i.e. catalepsy, righting reflex, ataxy, etc.) of opioid analgesics (fentanyl and its novel analogues
synthesised on the Faculty of Chemistry, Belgrade), antiepileptics (carbamazepine, oxcarbazepine and
its active metabolite, monohydroxy derivative-MHD and gabapentin), nonsteroidal anti-inflammatory
drugs used in human and veterinary medicine (flunixin meglumine, indomethacin), antidepressants
(amitriptyline, etc), as well as psychodelic agents (e.g. phencyclidine) in experimental animals (adult
rats, neonatal rats and adult mice) is planned. We are planning to evaluate the sites (central and/or
peripheral) and mechanisms of drugs action (the involvement of adrenergic, adenosine, opioidergic,
gabaergic receptors, the inhibition of the enzymes cyclooxigenase, and a role of L-arginine-NO
system), as well as drug-drug interactions - antagonism and synergism (both pharmacodynamic and
pharmacokinetic). Several animal models of acute and chronic pain (inflammatory, postoperative,
visceral and neuropathic pain) will be used, and mechanical, thermal and chemical pain stimuli will be
applied. c) Reactive oxygen and nitrogen species (ROS and RNS, respectively) are known to modulate
contractility of both skeletal and cardiac muscle. ROS and RNS are generated under various
pathological and physiological conditions (e.g. shock, respiratory failure, fatigue etc.). Different
modulators of NO system (NOS inhibitors, L-arginine, BH4), as well as other drugs influencing
inflammatory network (e.g. statins, antiepileptics etc.), will be tested in well established experimental
models regarding skeletal and/or heart muscle contractility. d) Also, a separate series of
investigations will be dedicated to pharmacoepidemiology/pharmacovigilance of antiinflammatory
drugs in clinical and outpatient settings (e.g. statins, NSAIL etc.). Finally, a separate series of
investigations will include pharmacokinetics of centrally acting drugs which may modulate
inflammatory status (e.g. carbamazepine and certain antidepressants). Research goal Taking into
account the presented subject, description and importance of the research (experimental models a-c,
as well as pharmacoepidemiological and pharmacokinetic analysis), the possible research goals will be
the following: a) improvement of glomerular and tubular function in rats subjected to I/R injury after
acute pretreatment with the statins, as well as the development of the appropriate subacute model of
such injury for further investigation; b)evaluation of sites (central and/or peripheral) and mechanisms
of drug action, as well as drug-drug interactions-antagonism and synergism (both pharmacodynamic
and pharmacokinetic) in order to improve pharmacotherapy of pain, especially neuropathic pain; c)
evaluation of possible improvement of respiratory and cardiac muscle failure. In addition,
pharmacoepidemiological as well as pharmacokinetic studies would help to re-establish principles of
rational pharmacotherapy of certain painful-inflammatory conditions.
Institution/University
Name of Institution/University
School of Medicine, University of Belgrade
Number of Employees
7481
Number of Researchers
7481
Country
Serbia and Montenegro
Postal Adress
Dr Subotica-starijeg 1
11129 Belgrade
Physiology
Research Team
Research Team Name
Department of Neuroscience, Split, Croatia
Individual Researcher
No
Research Team Leader Name
MD, PhD; Prof. Zoran Dogas
Research Team Members
Team Member 0
MD Zeljka Roje [[email protected]]
Team Member 1
MD Zavisa Colovic []
Team Member 2
MD, MSc Mladen Carev [[email protected]]
Team Member 3
MD, PhD; Prof. Goran Racic [[email protected]]
Team Member 4
MD, MSc Nenad Karanovic [[email protected]]
Team Member 5
MD Suzana Mladinov [[email protected]]
Team Member 6
BSc, Psychology; MSc Goran Kardum [[email protected]]
Team Member 7
MD Renata Pecotic [[email protected]]
Team Member 8
MD, PhD Maja Valic [[email protected]]
Team Member 9
MD Nikola Poljak []
Research Team Projects
Project Name
Neurotransmitters in the control of breathing
Project Leader
Prof. Zoran Dogas [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2002-01-01
Project End Date
2006-12-01
Project Partners
Project Summary
Breathing is a complex behavior which requires the coordinated activity of several muscle groups and
which must be precisely regulated by the brain via respiratory neurons to control arterial blood gases
and acid-base status within the narrow limits compatible with life. The main objective of the study is
to demonstrate the importance of roles of GABAergic, glutamatergic and serotoninergic receptors in
the process of neurotransmission-regulated action of premotor respiratory neurons, as well as
possible effects of the drugs acting at those receptors on sleep breathing disorders. We expect that
microinjection of GABAA antagonist bicuculline in the vicinity of the respiratory neuron will modulate
its discharge rate pattern as it was described in dogs, and has not been proven in rats (TonkovicCapin, 2001) and also that microinjection of GABAA channel blocker picrotoxin results in qualitatively
different effects (blocking of inhibition during inactive phase of neuronal activity vs. gain modulation,
Ðogaš et al., 1998). All of this would imply the complicated pharmacological regulation of GABA
neurotransmission and complex role of that neurotransmission in the modulation and control of motor
behavior during breathing. We do not expect that the gain modulation is modulated via the
benzodiazepine site which would suggest that the effects of activation of benzodiazepine site, as a
part of GABA receptor complex, are separated from the effects of GABA receptors in the control of
respiratory neurons. Thus, one can expect that benzodiazepine drugs do not effect the sleep
disordered breathing, and research on glutamate and serotonin drugs will demonstrate their roles.
Studying the control of breathing requires an intact brainstem and spinal cord circuitry together with
afferent input from the lungs. There is insufficient information about the central neural circuitry
underlying the reflex control of breathing to generate mathematical or computer models as
substitutes for animal experiments. Tissue culture experiments can not replicate the neural
connections required for the production of breathing. The only way to conduct investigation about
complicated function such as breathing is to use a whole animal model. This research may provide
information that will be helpful in understanding the neuronal control of breathing and
pharmacological treatment of patients with sleep disordered breathing such as opstructive sleep
apnea or prevention of sudden infant death syndrom.
Project Website
http://zprojekti.mzos.hr/zprojektiold/result
Physiology
Contact Person
Name
MD, PhD; Prof. Zoran Dogas
Email
[email protected]
Function
Vice Dean; Dept. of Neuroscience, Chair
Phone
+385 21 557 905
Fax
+385 21 557 955
Website
http://www2.mefst.hr/default.asp?ID=38
Research Team Objectives
Main Fields
Physiology
Anaesthesiology
Otorhinolaryngology
Research team is involved in studies on respiratory control during wakefulness, anesthesia and sleep.
Basic research performed on rats includes electrophysiology, microejection techniques,
neuropharmacology, and neuronal recordings from the respiratory related brain stem neurons.
Human studies are mainly done on breathing disorders during sleep (e.g., sleep apnea), as well as on
cognition and sleep.
Partners/Interests
researcher
industrial partner
Neurology
Psychiatry
Public health services
sleep diagnostics and therapy
Sleep disorders
Institution/University
Name of Institution/University
University of Split School of Medicine
Number of Employees
1500
Number of Researchers
1000
Country
Croatia
Postal Adress
Soltanska 2
21000 Split
Physiology
Research Team
Research Team Name
Laboratory for the physiology of circulation
Individual Researcher
No
Research Team Leader Name
MD, PhD Ines Drenjancevic-Peric
Research Team Members
Team Member 0
MD, PhD Ines Drenjancevic-Peric [[email protected]]
Team Member 1
MD Mario Gros [[email protected]]
Contact Person
Name
MD, PhD Ines Drenjancevic-Peric
Email
[email protected]
Function
Vice Dean for Science School of Medicine University of Osijek
Phone
+385 31 512 882
Fax
+385 31 512 833
Website
www.mefos.hr
Research Team Objectives
Main Fields
Physiology
Immunology and Immunohaematology
Control mechanisms of the blood flow through the tissue, functional and structural adaptation of
microcirculation (resistant arteries and arterioles), genetic factors in the development of
hypertension, interaction of various signalling systems in the cell and their common denominator
which regulates cell function (endothelial and vascular smooth muscle cell) and function of blood
vessels in response to different stimuli; the effect of physiological regulators of vascular function
(vasodilators and vasoconstrictors, oxygen and mediators of arachidonic acid), role of the reninangiotensin system in the control of vascular reactivity, dysfunction of the blood vessels in diabetes
mellitus and other microangiopathy, inflammation, aterosclerosis and sepsis, physiological
mechanisms of hyperbaric oxygen treatment
Partners/Interests
researcher
industrial partner
Physiology
laboratory of physiology, experience in vascular physiology, functional and genetic analysis,
mollecular biology techniques
role of oxygen in vascular control in health and disease
Institution/University
Name of Institution/University
School of Medicine University J. J. Strossmayer Osijek
Number of Employees
300
Number of Researchers
120
Country
Croatia
Postal Adress
J. Huttlera 4
31000 Osijek
Physiology
Research Team
Research Team Name
Neurophysiology Unit
Individual Researcher
No
Research Team Leader Name
Dr Andrea Cavaggioni
Research Team Members
Team Member 0
Dr Carla Mucignat [[email protected]]
Team Member 1
Dr Michela Bondi [[email protected]]
Team Member 2
Dr Alessandro Rubini [[email protected]]
Contact Person
Name
Dr Andrea Cavaggioni
Email
[email protected]
Function
Full professor
Phone
0039 049 827 5314
Fax
0039 049 827 5301
Website
unipd.it
Research Team Objectives
Main Fields
Physiology
Neurophysiology of olfaction Glioblastoma experimental model
Partners/Interests
researcher
Physiology
Endothelium physiology and pathology
Effect of cytokines in endothelium conditioning
Institution/University
Name of Institution/University
University of Padova
Number of Employees
6000
Number of Researchers
4000
Country
Italy
Postal Adress
Via 8 Febbraio 6
35130 Padova
Physiology
Research Team
Research Team Name
Physical Medicine and Rehabilitation
Individual Researcher
No
Research Team Leader Name
Mr. MD, PhD, Zmago Turk
Contact Person
Name
Mr. MD, PhD, Zmago Turk
Email
[email protected]
Function
Asist. Prof. of Physical Medicine and Rehabilitation
Phone
+ 386 2 234 56 01
Fax
+ 386 2 23 45 600
Website
www.mf.uni-mb.si
Research Team Objectives
Main Fields
Physiology
Physiology
Partners/Interests
researcher
industrial partner
Suitable
Physiology
POSSIBILITIES FOR COLLABORATIVE RESEARCH IN THE FIELD OF PHYSICAL THERAPY IN
COOPERATION WITH ASSOCIATED UNIVERSITIES COORDINATED BY MEDICAL UNIVERSITY OF GRAZ
Taking into consideration the rapid aging of Europe's population (24%) as well as in Republic of
Slovenia (20%) and other former Yugoslav republics (18 to 20%) it seems necessary to concentrate
our research efforts on medical fields of geriatrics and gerontology. Therefore we propose the
following projects/studies in the field of geriatrics: • Founding of centers for gerontology as a project
for complete nursing of the elderly, from domestic to institutional health care service. • Particularities
of hospitalization of the elderly. Analysis of specialities of hospitalization of elderly patients, means of
rehabilitation after injuries as a complete medical treatment. • Comparative analysis of life of the
elderly in nursing homes and founding of day health care services. Concerning the field of gerontology
we are currently researching nutrition and recreation habits of the elderly as well as ergonomic
obstacles. Other projects that can be carried out in the field of Physical and Rehabilitation Medicine:
a) Influence of low-frequency magnetic fields on human health (4 research projects already
completed) b) Influence of therapeutic electromagnetic fields on diseases of human locomotor system
and their treatment c) Thermal imaging of the human locomotor system – assessment of
inflammations, temperature variations and consequent diagnosis of diseases d) Treatment and
prolonged rehabilitation of patients with severe head traumas and diseases. CONCLUSION We see the
possibilities for our research in the fields of gerontology and geriatrics, integral treatment and
rehabilitation of human locomotor system, environmental influences on human health (especially
from an ergonomics point of view), physical therapy. Our research team consists of: prof. of Physical
Medicine and Rehabilitation, 3 teaching assistants, 4 doctoral candidates and a research assistant (in
the field of computer science). We offer the aforementioned research projects on behalf of Faculty of
Medicine, University of Maribor.
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Physiology
Research Team
Research Team Name
Physiology
Individual Researcher
No
Research Team Leader Name
Mr. PhD Marjan Rupnik
Contact Person
Name
Mr. PhD Marjan Rupnik
Email
[email protected]
Function
Professor of Physiology
Phone
+386 2 330 5854
Fax
+386 2 330 5853
Website
http://www.mf.uni-mb.si/fizio/rupnik.html
Research Team Objectives
Main Fields
Physiology
Partners/Interests
researcher
industrial partner
Suitable
Physiology
1. FUNCTION OF ENDOCRINE CELLS IN DISEASE AND COMPENSATORY PROCESSES Our main
expertises are electrophysiology (patch-clamp) and optophysiology (wide-field, confocal and twophoton microscopy) on living endocrine tissue slices. The endocrine tissue slice preparation was due
to its complexity rarely used tool to study endocrine function. We have recently established a novel
preparation to study the endocrine function in pancreas, its endocrine part being vital for the control
of the blood glucose and its dysfunction being critical in severe diseases, like diabetes mellitus. Our
preparation is the first, where the hormone (e.g. insulin) release and electrical activity can be
checked in the intact tissue environment with a single cell resolution and where important cellular
characteristics, like ion channels and metabolic activity can be assessed together with the secretory
competence. We expect that the use of genetically modified animals and animals which develop
sickness due to planned inbreeding will give us key information of the defects causing an endocrine
disease and help us develop new methods of treatment and diagnostics. We propose a research
project to define the molecular basis of type-2 diabetes mellitus or similar endocrine disorders. By
applying measurements of secretory competence, electrical activity and metabolism in endocrine cells
in tissue slices by a combination of electro- and optophysiology in whole pancreas slices we are
willing to cooperate in characterization of putative secretory machinery dysfunction and
compensatory processes in endocrine tissues due to specific genetic manipulations.
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Physiology
Research Team
Research Team Name
Prevention of obesity and cardiovascular risk factors in
children
Individual Researcher
Yes
Research Team Leader Name
Prof. dr. Mirjana Pavlović
Research Team Projects
Project Name
Capacity Development in Nutrition in Central and Eastern
Europe
Project Leader
Mirjana Pavlovic, MD, PhD, RPHNutr.
Contact Person
Name
Mirjana Pavlovic, MD, PhD, RPHNutr.
Email
[email protected]
Function
Associate Research Professor, CEE Network Coordinator
Phone
+381 24 548398 / +381 11 303 1997
Fax
+381 24 548398 / +381 11 303 1997
Website
www.imi.bg.ac.org
Research Team Objectives
Main Fields
Physiology
Cardiovascular risk factors
Obesity
Nutrition
Atherosclerosis
Primary prevention
Many reports review the evidence that the precursors of atherosclerosis begin in childhood. The
majority of risk factors are connected with eating habits and life style patterns already adopted in
childhood and youth. Overweight in childhood has been associated with increased risks of
hypertension, adverse lipid profiles, type II diabetes, and early atherosclerosis lesions, as well as
increased risks of adult obesity and obesity related morbidities and mortality in adulthood. The
correlation between risk factors level found in early youth and their later values is the most
important fact for primary prevention CVD in childhood.
The project includes primary prevention program of CVD risk factors integrated in paediatric care of
children. It implies both high risk and population based approaches. At the population level
recommendations will be linked with advice to implementing the Step 1 Diet low in saturated fat and
cholesterol for all children over 2 years of age, with promotion of healthy nutrition and lifestyle and
physical activity programs in preschool childcare centres and schools. Participation of the whole
society is important. Individual level involves assessment and early identification of the children with
obesity, hypertension, sedentary lifestyle and family history for selective screening of lipid
parameters in the course of routine health care. It would be important also to identify positive
aspects to be used in intervention. According to observed risk factors on individual level the
recommendations are based on promotion of physical activity and nutritionally adequate diets with
balanced intake of calories, vitamins and minerals, this as a Step 2 Diet with further reduction in
cholesterol and saturated fat with nutrition professional consultation.
Primary prevention in childhood can be more efficient and cost effective in preventing CVD than in
adulthood. The project includes continuous longitudinal growth status monitoring by anthropometry,
of child obesity through various ages at individual level. It will also imply identifying hypertension,
registering type of lifestyle (including physical activity, and smoking of parents etc.), family history
of health and disease, selective screening of biochemical parameters (lipids mainly) in the course of
routine health care. Individuals will also get professional advice on how to change lifestyle based on
recommendations on physical activity and nutritionally adequate diets with balanced intake of
calories, vitamins and minerals, including reduction in intake of saturated fat.
At the population level recommendations will be linked with advice impacting on lifestyle, also
including physical activity promotion programs in preschool childcare centres and schools.
Participation of the whole society is important. Here the aim is to change nutrition and lifestyle in
whole populations over 2 year, including dietary quality of families, by for example providing
examples of nutritionally adequate diets. It may also entail assessing nutritional quality of menus,
Physiology
education in healthy nutrition of children, parents, cooks, teachers and other actors in the society.
This can be done by using mass-media, engage in celebrations of the World Food Day which takes
place every October, and take the opportunity of other ongoing programmes such as National Month
of Healthy Nutrition for preschool and schoolchildren.
The plan will include assessing the quality of diet; this will be compared to the nutritional needs of
individuals, diet consulting counselling (including individualised menus) and counselling on healthy
lifestyle. Adequate software will be developed in addition to already available softwares.
For using the data from monitoring and evaluation appropriate software applications for data
analysis at the individual and population level is needed. Adequate software will be developed in
addition to already available sofwares.
Towards the end of the project a number of tools for local and national use will be developed. That
may include:
•
•
•
Integrate primary prevention program of CVD risk factors into pediatric care of children,
implying two complementary approaches, "individual-high risk strategy" and a "population based
- health promotion strategy".
Establish a model for primary prevention program of
cardiovascular diseases
in
a
regional/national strategy for primary prevention of the risk factors causing chronic noncommunicable diseases, primarily cardiovascular, complying with the WHO “Global Strategy on
Diet, Physical Activity and Health“.
The project will focus on implementation of a prevention programme, and emphasise dissemination
results in the selected communities, but also share information with other countries in a establishe
Network for Capacity Development in Nutrition in CEE.
•
•
Framework for nutrition education program in curricula ("Health promoting schools").
Broad plans/frameworks for action and suggestions for optimal meals for children in
kindergartens and schools, in cooperation with the food producers (for example: a milk based
product for each child and fruit and vegetable as snacks in every school).
•
Improving the quality and the choice of the meals in school canteens "Healthy school canteens".
•
Raise the issue of responsible marketing of foods, snacks and soft drinks for children.
•
During the implementation of this project
are planning cooperation with relevant State
ministries (health, education and sport, agriculture, science and environment), food industrySMEs, municipalities, media, consumer associations, NGO-s, and the private sectors, with the
aim of initiating a process towards a ’’National Nutrition Action Plan’’ according to the principles
developed by the WHO Regional Office for Europe.
Throughout the project community participation will be actively promoted. That implies engaging the
whole community in promoting healthy lifestyles and adequate nutrition (such as in campaigns,
festivals, workshops, seminars, lectures and public education through the media, press conferences,
Internet communication, video films and brochures).
Partners/Interests
researcher
Cardiovascular risk factors
Obesity
Nutrition
Atherosclerosis
Primary prevention
Objectives:
1. Integrate primary prevention program of CVD risk factors into paediatric care of children,
implying two complementary approaches, "individual-high risk strategy" and a "population based
- health promotion strategy".
2. Establish a model/framework for primary prevention program of cardiovascular diseases in a
regional/national strategy for primary prevention of the risk factors causing chronic noncommunicable diseases, primarily cardiovascular, complying with the WHO “Global Strategy on
Diet, Physical Activity and Health“.
3. Monitoring of the nutritional status in children for early detection of the risk factors for
cardiovascular diseases (obesity, hypertension, hyperlipidemia, positive family history and
inadequate dietary intake and physical activity).
4. Establish a national database on cardiovascular risk factors, dietary patterns and quality,
nutritional status (measured by anthropometry) and level of physical activity in children.
5. Create a process for developing regional/national and local strategies and food and nutrition
policies, complying with the “Nutrition Action Plan” for Europe by WHO.
Expected results
The project will focus on implementation of a prevention programme, emphasising dissemination of
Physiology
results from assessments to the communities involved. However, sharing information with other
countries in a established Network for Capacity Development in Nutrition in CEE would be
important.
In shorter term the project will monitor nutritional status of children in order discover early
overweight and obesity, and the nutritional risk factors on individual and/or at population level for
preventing obesity and cardiovascular risk factors, and for health promotion activities.
In the long term the expected result is to reduce overweight, obesity and cardiovascular risk factors,
and an improved nutritionally quality of diets, and increased physical activity in children assessed
against the criteria of the WHO Global Strategy on Diet, Physical Activity and Health.
Institution/University
Name of Institution/University
Department of Nutrition and Metabolism, Institute for Medical
Research, University of Belgrade
Number of Employees
Number of Researchers
Country
Serbia
Postal Adress
Tadeusa Koscuskog 1, PO Box 102
11000 Belgrade
Physiology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Prof. Dr. Barbara Obermayer-Pietsch
Contact Person
Name
Prof. Dr. Barbara Obermayer-Pietsch
Email
[email protected]
Function
ass. Prof.
Phone
+43 316 385 2934
Fax
+43 316 385 3428
Website
http://www.meduni-graz.at/endo-nuklearmedizin
Research Team Objectives
Main Fields
Physiology
gender-based medicine
Partners/Interests
gender-based medicine
Institution/University
Name of Institution/University
Clinical Department for Endocrinology and Nuclear Medicine,
Medical University of Graz
Number of Employees
Number of Researchers
Country
Austria
Postal Adress
Auenbruggerplatz 15
8036 Graz
Psychiatry
Research Team
Research Team Name
Molecular and biochemical markers of psychiatryc disorders
Individual Researcher
No
Research Team Leader Name
MD PhD Dalibor Karlovic
Research Team Members
Team Member 0
MD Marko Martinac [[email protected]]
Research Team Projects
Project Name
Analize of serum lipids in psychiatryc disorders
Project Leader
MD PhD Dalibor Karloviæ [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2001-11-11
Project End Date
2006-03-11
Project Partners
Project Summary
Project Website
Contact Person
Name
MD PhD Dalibor Karloviæ
Email
[email protected]
Function
Psychiatryst, reearcher
Phone
+38513787345
Fax
+38513787345
Website
[email protected]
Research Team Objectives
Main Fields
Psychiatry
Pharmacology
Clinical chemistry
Main interest in psychopharmacology, clinical laboratory data in psychiatry (especialy lipids
metabolism, endocrinology and molecular diagnostics)
Partners/Interests
researcher
Psychiatry
Polimorphisms of variety genes in schizophrenia, bipolar disorder and depression
Institution/University
Name of Institution/University
University Hospital "Sestre milosrdnice", Dept. of Psychiatry
Number of Employees
10
Number of Researchers
5
Country
Croatia
Postal Adress
Vinogradska cesta 29
10000 Zagreb
Psychiatry
Research Team
Research Team Name
TELEMEDICINE & TELEPSYCHIATRY AMDA INTERNATIONALAMDA RS BA
Individual Researcher
No
Research Team Leader Name
Prof dr sc med Milan Stojakovic
Research Team Members
Team Member 0
Dr sc Zoran Mavija [[email protected]]
Team Member 1
Mr sc Ozren Kordic [[email protected]]
Team Member 2
Prof Dr sc med Zdravko Maric [[email protected]]
Contact Person
Name
Prof dr sc med Milan Stojakovic
Email
[email protected]
Function
president
Phone
++387 51 302 864
Fax
++387 51 302 864
Website
home.blic.net/misos
Research Team Objectives
Main Fields
Psychiatry
Internal Medicine
Information Technology, Statistics, Documentation
RESEARCH ACTIVITIES:TELEMEDICINE.. E-health,mental health, telepsychiatry,e/mail
psychoconsalting,forensic psychiatry
Partners/Interests
researcher
industrial partner
Information Technology, Statistics, Documentation
Psychiatry
Public health services
DESCRIPTION OF REQUESTED PARTNER PROFILE: EXPECT interest for implementation of
TELEMEDICINE.. & interest for implementation of telepsychiatry services in Bosnia.
TELEMEDICINE & telepsychiatry
Institution/University
Name of Institution/University
AMDA international /BA
Number of Employees
5
Number of Researchers
3
Country
Bosnia and Herzegovina
Postal Adress
K PETRA PRVOG 115 115
78000 home.blic.net/misos
Public Health Services
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
MD Aleksandar Dzakula
Contact Person
Name
MD Aleksandar Dzakula
Email
[email protected]
Function
Research Fellow
Phone
+385 91 582 88 92
Fax
+385 1 4590 275
Website
www.snz.hr
Research Team Objectives
Main Fields
Public health services
Social medicine
Epidemiology
Organization of helath care services; health care policy; health care management
Partners/Interests
researcher
Public health services
Social medicine
Epidemiology
Able to analyze health care sector and willing to manage internationla projects
Rationalizing the hospital sector - policy driven research
Institution/University
Name of Institution/University
University of Zagreb - Andrija Stampar School of Public Health
Number of Employees
70
Number of Researchers
50
Country
Croatia
Postal Adress
Rockefellerova 4
10000 Zagreb
Public Health Services
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
senior analyst Marilena Ianculescu
Research Team Projects
Project Name
Informational Centre of Dermathology
Project Leader
senior analyst Marilena Ianculescu [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-15
Project End Date
2008-12-15
Project Partners
"Carol Davila" University of Medicine and Pharmacy, Bucharest,
Romania
Project Summary
The web-based system will become an efficient tool for promoting good health and a healthy lifestyle,
it will facilitate the co-operation between doctor and patient. Expected results: • a web-based modern
system for informing citizens in the dermatovenereology domain having as central point a relational
databases system; • an efficient and multifunctional tool for dissemination of the the
dermatovenereology domain; • increasing the accessibility degree of the quality information
regarding health and prophylaxis; • libraries with primary medical information and medical
publications; • monitoring, educational and evaluation applications.
Project Website
www.cid.ro
Project Name
A Complex Integrated System for Health Education and
Disease Prevention
Project Leader
Prof. dr. eng. mat. Adriana Alexandru [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2005-10-03
Project End Date
2005-09-30
Project Partners
"Carol Davila" University of Medicine and Pharmacy, Bucharest,
Romania Valahia University, Targoviste, Romania S.C. Software ITC
S.A. Net Clip S.R.L.
Project Summary
The proposed project, due to its tackled approach, finds a place among the thematic fields S/T of
CEEX 2005, aiming to improve the capacity of preventing diseases, to facilitate an early diagnosis and
to realize health information space for knowledge discovery. Maintaining a state of good health for the
population, adopting a healthier lifestyle, with special implications on long term in social and
economic plan, can be made with the help of a sanitary culture, and information technologies are an
extraordinary means to promote education in the public health domain. Making a complex integrated
system like this one is a shared responsibility that implies cooperation and creating partnership
among decision-makers in the health domain, health professionals and computer specialists. The
proposed system will contain information organized in an relational database system about
immunization, screening, prophylaxis strategies and methods, risk factors, occupational health,
environmental factors, institutions in the public health field, educational models for different kinds of
communities, a library with medical information and publications, and applications made to facilitate
the dissemination of the analysis from the territory and the collaboration among the institutions
having a role to play in the public health system (experience exchange, training, partnership
proposals, promoting national and international programmes in the public health domain).
Project Website
www.edusan.ro
Contact Person
Name
Marilena Ianculescu
Email
[email protected]
Function
senior analyst
Public Health Services
Phone
+40-744 777967
Fax
+40-21-224 11 21
Website
www.ici.ro
Research Team Objectives
Main Fields
Public health services
Information Technology, Statistics, Documentation
Advanced information technologies for the information society - networks and data base systems in
public health domain Integrated solutions for public services for Health care e-Business
Partners/Interests
industrial partner
Public health services
Information Technology, Statistics, Documentation
public health services IT
Public health education using IT.
Institution/University
Name of Institution/University
National Research Institute for R&D in Informatics
Number of Employees
215
Number of Researchers
124
Country
Romania
Postal Adress
Bd. Maresal Averescu 8-10
01145 Bucharest
Public Health Services
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
PhD Ljiljana Tasic
Research Team Projects
Project Name
Healt promotion and dessisses prevention of women health
Project Leader
professor Ljiljana Tasic [[email protected]]
Project Funding Agency
Pfizer, farmalogist, Shering
Project Budget
14.000 €
Project Start Date
2006-04-01
Project End Date
2007-03-31
Project Partners
Faculty of Pharmacy, department of social pharmacy and Public
Pharmacy Institution of Belgrade
Project Summary
healt promotion in three women population group (young, meadle and old); educate health providers
(pharmacist and doctors) for the skills of health promotion and health life stiles changes; prevent
sexual, cardiovasular, depression and osteoporosis in population of women in Belgrade -urban and
rural area; create web and net platform and partnership with patients, public, media, and health
professionals
Project Website
ww.zdravljezena.org
Contact Person
Name
PhD Ljiljana Tasic
Email
[email protected]
Function
profesor
Phone
+381113970379
Fax
+381113974349
Website
www.pharmacy.bg.ac.yu
Research Team Objectives
Main Fields
Public health services
Information Technology, Statistics, Documentation
Pharmacy
qualty of health care; pharmaceutical care; pharmacoepidemiology and pharmacoeconomy; drug
policy; e-health; e-pharmacy
Partners/Interests
researcher
Information Technology, Statistics, Documentation
Pharmacy
Public health services
experience in public health and drug policy, drug database and e-health
improvement of health and drug public information sources
Institution/University
Name of Institution/University
University of Belgrade,Facultz of Pharmacy
Number of Employees
151
Number of Researchers
266
Country
Serbia and Montenegro
Postal Adress
V.Stepe 450
11221 Belgrade
Public Health Services
Research Team
Research Team Name
Electronic patient records throughout Europe
Individual Researcher
No
Research Team Leader Name
Diana Szedlacsek
Contact Person
Name
Diana Szedlacsek
Email
[email protected]
Phone
+40 21 22 835
Fax
+40 21 22 835
Research Team Objectives
Main Fields
Public health services
Information Technology, Statistics, Documentation
electronic health recordings training of medical doctors European patient care
Partners/Interests
researcher
Public health services
Information Technology, Statistics, Documentation
The purpose of the project aims at: Educating the medical staff in the new member and associate
candidate countries, with the trends for electronic patient records already emerging in some of the EU
states. Performing the first steps towards the analysis of the state-of-the-art in this extreme
fragmented domain and towards the unification of electronic registration systems already existing
throughout Europe – at least at a first administrative step. The expected results are: 1. Increased
willingness of the medical staff to cooperate not only throughout regional areas, but throughout the
whole European domain. 2. Increased ease to consult patients throughout Europe. 3. Increased
efficiency of the administrative (financial, IST, recording) aspects of the medical care. Impact:
Throughout Europe, the problem of electronic patient records is analyzed and steps are performed
slowly enough. Therefore, unification of the efforts in this respect is sought to have a tremendous
impact upon the unified European medical care system. The interests and points of views of the
medical doctors are still restrained to personal and national level. Through the unification of the
recording systems, a wider view is sought for the medical doctors. Contribution to the EU or regional
policy: The proposed project aims at meeting social needs and catalyze the delivery of
European/Regional policy objective(s), by generalizing the electronic recording of patients, by easing
the patient care throughout Europe and by training the medical doctors in using such generalized
systems for patient care. European research potential: Due to numerous attempts to introduce and
generalize an electronic patient recording system, it is to be inferred that the unification of research
efforts throughout Europe – and especially in the new member and in the associate candidate
countries -, is going to mobilize a strong potential for disseminating and converting the results into
social and economical benefits. Since only partial recording systems exist and since they are not by
all means interusable throughout Europe, it is also clear that the proposed project aims at building on
past and current investments and successes – at least at national level – successes which should be
materialized into a European widespread such system. European added value: Since such generalized
patient recording systems are sought to be used throughout Europe, it is also obvious that the project
considers additional public funding to be justified by the externalities and wider benefits from the
research and by the need to attract increased public and private investments. Also, the need for
cooperation between medical, IST, accounting, administrative staff involved in medical care ensures
multidisciplinarity and critical mass of scale and scope. The projects, as it was presented above aims
to overcome fragmentation and unnecessary duplication, lack of connections and of interoperability;
to complement other intergovernmental, national and private actions; to address European level
problems.
Institution/University
Name of Institution/University
SME Bio-Consult service s.r.l.
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Romania
Postal Adress
Sos. Stefan cel Mare 14
20141 Bucharest
Public Health Services
Research Team
Research Team Name
Traditional medical prevention and care throughout Europe
Individual Researcher
No
Research Team Leader Name
Diana Szedlacsek
Contact Person
Name
Diana Szedlacsek
Email
[email protected]
Function
Phone
+40 21 22 835
Fax
+40 21 22 835
Website
Research Team Objectives
Main Fields
Public health services
Social medicine
Partners/Interests
researcher
Public health services
Social medicine
The objectives of the present proposal are: To build on existing European empirical, traditional
medical practices, in order to obtain an efficient system for preventing and combating different types
of diseases (including major ones). To ease the dissemination of healthcare empirical (national or
regional) knowledge systems in order to increase public awareness regarding the necessity to get
more involved into personal and societal medical care. To increase the opportunities for rediscovering traditional, European values in the medical field. To widen the research connected to such
traditional experience in the medical practices, in order to enlarge both medical and public experience
in preventing and combating diseases, as well as to gain new, richer insights into the health sciences.
Expected results: Increased public awareness regarding the necessity for self-care Eased European
and national budgets from unnecessary medical costs, where traditional remedies might do Widened
views in the health sciences research and medical practice fields Widened consciousness of public and
medical staff regarding a common European tradition in the medical practice Increased research into
European traditional practices in the health sciences Translation of national and regional topics in the
field of healthcare Impact: At European level: increased efficiency of both traditional and classical
systems for medical care, improved public and medical consciousness related to traditional valuable
practices, widened experience in health research topics (through widened views), improved European
specific conscious about own values. At regional levels: same as above, plus increased satisfaction
towards promotion of regional values in health care and sciences. At national level: same as above,
plus widened insight into the European practices, applicable at national levels. At personal level:
increased conscience of the European citizenship and of a wider European practice in self-care,
increased self-esteem as European citizen and improved health status through use of specific,
European (therefore, better physiologically adapted) care systems. Contribution to the EU or regional
policy: The EU policy aims at a dynamic and competitive knowledge-based economy, which is to be
reached through the present project – in the health care sector, through obtaining new remedies and
practices destined to improved health, through lowering the burden of classical healthcare by using
traditional, non-conventional, cheap and at-hand practices, products and self-care systems. This view
is currently gaining more and more importance throughout the world and the US and Asian countries
have already made serious progresses in this direction. Due to the above, the aim of sustainable
economic growth is also to be reached through the application of the results of the project. Since the
European/regional policies aim at generating both a European high-quality, specific value intended to
the global exchanges, the present project fits to such objective. Also, the increased awareness about
the traditional practices in healthcare aims at boosting the European research into new, valuable
directions, already undertaken, for example, by Asian countries in their own directions. European
research potential: As concerns the views from the research community and industry, mention should
be made from the fact that the international market is increasingly invaded by North-American
products and Asian traditional remedies and practices, which already form nowadays a coherent
system, even if they are (slightly) divided into more main directions. Therefore, European specific
remedies not only might improve the health status of Europeans (through the use of specific
remedies, adapted to physiologically specific needs), but will boost the research and industry into this
emerging domain of the global market. Past investments are already successful in this field, at
Public Health Services
regional levels and this experience should be shared among European countries in view of its
generalization. Research in this direction is only emerging in Europe, and serious efforts should be
made in order to encompass the advance of other countries and regions, though success in the field
can already be witnessed in Europe. Yet, a stronger impulse must be conveyed to the area, in order
to increase the potential for excellent research and technological development and for disseminating
and converting the results into social and economic benefits. European added value: Current public
support for tradition in general and for traditional remedies and practices in particular, is continuously
increasing, and a best approach would be to meet the increasing needs of the public for knowledge in
this field, by offering sound, correct research-supported data at all necessary levels. It is obvious that
both public and private investments are duly justified in a field where European research and market
are slightly backwards – as compared with other regions and states. Also, such an approach will offer
the opportunity for European centers of excellence through collaborative research, needed for the
high-throughput requested by the field. Such a research domain will require the necessary multidisciplinarity and critical mass, due to the fact that, at present, such remedies and practices are
widely known at national or regional level at most, not at the European level. The involvement of
European research in the above-mentioned direction will enhance visibility of European research
excellence, taking into account the current international trends and efforts.
Institution/University
Name of Institution/University
SME Bio-Consult service s.r.l.
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Romania
Postal Adress
Sos. Stefan cel Mare 14
20141 Bucharest
Radiology
Research Team
Research Team Name
Magnetic Resonance Research Department
Individual Researcher
No
Research Team Leader Name
MD Nicolae Bolog
Research Team Members
Team Member 0
MD Nicolae Bolog [[email protected]]
Team Member 1
MD Irinel Oancea [[email protected]]
Team Member 2
MD Angelica Mangrau [[email protected]]
Team Member 3
Physicist Claudiu Ungureanu [fcla[email protected]]
Contact Person
Name
MD Nicolae Bolog
Email
[email protected]
Function
Head of Radiology and Magnetic Resonance Department
Phone
+40 21 5992300 / 304
Fax
+40 21 5992308 / 304
Website
www.ehmri.ro
Research Team Objectives
Main Fields
Radiology
Information Technology, Statistics, Documentation
Clinical Research in Magnetic Resonance Imaging focused on abdominal and musculoskeletal imaging,
and soft-tissue tumors
Partners/Interests
researcher
Radiology
Information Technology, Statistics, Documentation
special interest in development of new MR technology such as MR elastography and in development of
new contrast agents for MR imaging.
Clinical Research in Magnetic Resonance Imaging focused on abdominal and musculoskeletal imaging
and soft-tissue tumors
Institution/University
Name of Institution/University
Emergency Hospital Bucharest
Number of Employees
2707
Number of Researchers
87
Country
Romania
Postal Adress
Calea Floreasca 8
01446 Bucharest
Radiology
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Prof.dr Lidija LINCEDER
Research Team Members
Team Member 0
Dr sci. Nermina OBRALIĆ
Team Member 1
Dr sci. Šerif BEŠLIĆ
Team Member 2
Mr .sci. dr Nermina KANTARDŽIĆ
Team Member 3
Dr. sci. Hiba BAŠIĆ
Team Member 4
Mr. sci. dr Amela MORNJAKOVIĆ
Team Member 5
Dr Sabina PREVLJAK
Team Member 6
Vegar S.
Team Member 7
Dr Sabina PREVLJAK
Team Member 8
Dr. sci. Semir BEŠLIJA
Team Member 9
Sefic I.
Team Member 10
Mirela Kalamujic
Contact Person
Name
Prof.dr Lidija LINCEDER
Email
[email protected]
Function
Head of the Institute of Radiology and Onkology
Phone
+33 663 742 / 743
Fax
+33 203 670
Website
www.mf.unsa.ba
Research Team Objectives
Main Fields
Radiology
MRI
super paramagnetic iron oxide
lymphangiography
oncology
Partners/Interests
Evaluation of MRI lymphangiography in diagnostic of oncology patients (Super
paramagnetic Iron Oxide enhanced MRI lymphangiography)
Background: Status of regional lymph nodes has important significance for therapy and prognosis of
newly diagnosed cancers. The presence of nodal metastases determinates therapeutic options and it
is crucial to have this information to make decision about therapy.
Further more nodal metastases indicates worse prognosis, and thus is important in follow up of
oncology patients.
Current imaging modalities rely mostly on size and morphologies of the lymph nodes. Metastases can
be present in non enlarged nodes, and not all enlarged nodes are malignant. Super paramagnetic
Iron Oxide enhanced MRI can overcome some of limitations in the existing imaging modalities and
provide much needed staging information before the therapy and in the follow up of the cancer
patients
Aim: To evaluate characterization of lymph nodes in native MRI lymphangiography versus super
paramagnetic iron oxide enhanced MRI lymphangiography in diagnostic of the oncology patients.
Materials: The patients with histology of malignant tumors will undertake MRI imaging before and 24
hours after receiving optimal dose of super paramagnetic iron oxide. Vital signs, serum and urine
levels and eventual adverse events will be monitored. Qualitative nodal architecture, size and signal
intensity changes will be assessed in T1-, T2-, and T2* weighted images in native and enchanted
MRI.
The MR imaging super paramagnetic iron oxide appears to be effective for characterization of lymph
nodes in patient with various primary tumors and it will be very important to check significance and
sensibility of this new imaging method.
Radiology
Institution/University
Name of Institution/University
Medical Faculty of Sarajevo, University of Sarajevo
Number of Employees
Number of Researchers
Country
Bosnia and Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Radiology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Dr. Manuela Aschauer
Contact Person
Name
Dr. Manuela Aschauer
Email
[email protected]
Function
Phone
+43 316 385 3850
Fax
Website
http://www.meduni-graz.at/radiologie/
Research Team Objectives
Main Fields
Radiology
Partners/Interests
Analysis of MR study data and/or joint studies in the field of MRA
Institution/University
Name of Institution/University
Department of Radiology, Medical University of Graz
Number of Employees
Number of Researchers
Country
Austria
Postal Adress
8063 Graz, Auenbruggerplatz 9
8036 Graz, Tel.
Radiology
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Univ.-Prof. Dr.med.univ. Franz Ebner
Contact Person
Name
Prof. Franz Ebner
Email
franz.e[email protected]
Function
Phone
+43 316 385 3271
Fax
Website
http://www.meduni-graz.at/radiologie/
Research Team Objectives
Main Fields
Radiology
Partners/Interests
High-Field MR – experimental or preclinical work with volunteers
Institution/University
Name of Institution/University
Department of Radiology, Medical University of Graz
Number of Employees
Number of Researchers
Country
Austria
Postal Adress
Auenbruggerplatz 9
8036 Graz
Social Medicine
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
MD, MSc, PhD, specialist of pediatrics Aida Mujkic
Contact Person
Name
MD, MSc, PhD, specialist of pediatrics Aida Mujkic
Email
[email protected]
Function
Assistant professor
Phone
+38514554959
Fax
+38514566777
Website
www.snz.hr
Research Team Objectives
Main Fields
Social medicine
Paediatrics
Public health services
The field of social pediatrics which means all the influences on child's health (public health aspect of
family planning, child growth and development, nutrition,social relations towards children, special
interest for child injury prevention)
Partners/Interests
researcher
industrial partner
Paediatrics
Social medicine
Public health services
The researcher: experience in the scientific research and analysing data The industrial partner:
motivation to invent new or change curent products to fulfill safety requirements
The public health project for parents of preschool chidren with the aim to educate them about injury
prevention and safety promotion.
Institution/University
Name of Institution/University
Zagreb University-School of medicine-A. Stampar SPH
Number of Employees
100
Number of Researchers
40
Country
Croatia
Postal Adress
Rockefellerova 4
10000 Zagreb
Surgery
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Professor, MD, PhD Gojko Buljat
Contact Person
Name
Professor, MD, PhD Gojko Buljat
Email
[email protected]
Function
Head, University Hospital of Traumatology Zagreb
Phone
+385 1 46 10 363
Fax
+385 1 46 10 365
Website
www.trauma.hr
Research Team Objectives
Main Fields
Surgery
Anatomy
Neurology
spinal injuries and diseases spinal surgery
Partners/Interests
researcher
industrial partner
Surgery
Radiology
Therapeutics
experince with algorithms and national registers adequate computer programs/databases
spinal injuries and diseases - diagnostic and treatment algorithms
Institution/University
Name of Institution/University
University Hospital of Traumatology Zagreb
Number of Employees
452
Number of Researchers
18
Country
Croatia
Postal Adress
Draskoviceva 19
10000 Zagreb
Surgery
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Dozent Darko Anticevic
Research Team Members
Team Member 0
MD PhD Tomislav Djapic [[email protected]]
Team Member 1
MD Marko Bergovec [[email protected]]
Team Member 2
MD Bostjan Baebler []
Team Member 3
MD PhD Professor Ingeborg Barisic [[email protected]]
Team Member 4
PhD biol. Professor Biserka Nagy [[email protected]]
Team Member 5
PhD biol. Inga Marijanovic [[email protected]]
Team Member 6
MDPhD Professor Slobodan Vukicevic [[email protected]]
Team Member 7
PhD Lovorka Grgurevic [[email protected]]
Contact Person
Name
Dozent Darko Anticevic
Email
[email protected]
Function
pedaitric ortohopedic surgeon, staff member
Phone
481-9911
Fax
2303-119
Website
www.mef.hr
Research Team Objectives
Main Fields
Surgery
Genetics
Paediatrics
Paediatric orthopaedic rare bone and joint diseases; Osteogenesis imperfecta; Skeletal Dysplasias;
Bone regeneration
Partners/Interests
researcher
Genetics
Paediatrics
Surgery
with research and clinical experience with osteogenesis imperfecta
Expression of gene for typ I collagen in patients with osteogenesis imperfecta Genomic and proteomic
analysis of biomarkers in biological fluids of rare bone diseases
Institution/University
Name of Institution/University
Dept Ortho Surg (Ped Ortho Unit), School of Medicine, ZagUniv
Number of Employees
700
Number of Researchers
380
Country
Croatia
Postal Adress
Salata 3
10000 Zagreb
Surgery
Research Team
Research Team Name
Department of Abdominal Surgery I Lymphatic Mapping and
Sentinel Node Biopsy in Digestive System Neoplasms
Individual Researcher
No
Research Team Leader Name
Doc. dr. sc. Matija Horzic
Research Team Members
Team Member 0
Mr. sc. Mario Kopljar [[email protected]]
Team Member 1
Doc. dr. sc. Leonardo Patrlj []
Team Member 2
Mr. sc. Kristijan Cupurdija []
Team Member 3
Dr Djana Vanjak-Bielen []
Team Member 4
Dr. Domagoj Vergles []
Team Member 5
Dr. Zeljko Lackovic []
Contact Person
Name
Mr. sc. Mario Kopljar
Email
[email protected]
Function
Surgery
Phone
+385 91 582 7446
Fax
+385 1 290 3624
Website
www.kbd.hr
Research Team Objectives
Main Fields
Surgery
Anatomy
Genetics
Malignant diseases of the digestive system represent important medical, social and especially public
health issue in Croatia and in almost all countries of the Western civilization. Their most important
feature is high mortality and morbidity of certain types of cancer, which is determined mostly by the
quick dissemination of the primary tumor. Malignant tumors of the digestive system are among the
most common malignant diseases in general, and the incidence of certain digestive tract cancers is
increasing. Broadening of the general knowledge about the growth and dissemination of malignant
tumors of the digestive system represents the basis for their successful surgical treatment. Since
surgery is successful only in the early stages of cancer development, and mortality and morbidity
caused by these tumors and their treatment represent significant medical and social problem, the
research of growth and dissemination of the cancers of the digestive tract are today of great
significance. This planned research therefore aims to broadening the knowledge of routes of
spreading of the digestive tract tumors by intraoperative detection of tumor-infiltrated lymph nodes in
order to enable the removal of potential lymphatic dissemination and therefore increase the radicality
of surgical treatment. Curative treatment of solid malignancies is possible only by surgical resection
of the primary tumor and the removal of all potential routes of lymphatic spread. The most common
site of metastases from the tumors of the digestive tract are lymph nodes. Due to rich lymphatic
drainage of the digestive tract, there is a large number of routes of lymphatic spread from tumors
emerging in digestive tract. Since the knowledge about these routes of lymphatic drainage is
mandatory for achieving adequate surgical radicality, precise intraoperative determination of lymph
node infiltration and routes of lymphatic spread enable focused extension of lymphadenectomy to
those areas that are not included in the standard range of lymphadenectomy, thus significantly
increasing surgical radicality. Also, in the later stage of research, intraoperative detection of lymphatic
flow from the primary tumor may lead to sparing those areas where no lymphatic spread from the
primary tumor was observed. Results of this research should increase the number or truly radical
resections, that should be made evident through the decrease in recurrence rates and increase in
overall and disease free survival.
Partners/Interests
researcher
Surgery
Surgery
Institution/University
Name of Institution/University
University Hospital Dubrava
Number of Employees
n.a.
Number of Researchers
Na.a
Country
Croatia
Postal Adress
Av. G. Suska 6
10000 Zagreb
Surgery
Research Team
Research Team Name
Department of Abdominal Surgery I - Quality of life
measures in surgery
Individual Researcher
No
Research Team Leader Name
Doc. dr. sc. Matija Horzic
Research Team Members
Team Member 0
Dr. Domagoj Vergles []
Team Member 1
Dr. Zeljko Lackovic []
Team Member 2
Dr. Djana Vanjak-Bielen []
Team Member 3
Mr. sc. Kristijan Cupurdija []
Team Member 4
Doc. dr. sc. Leonardo Patrlj []
Team Member 5
Mr. sc. Mario Kopljar [[email protected]]
Contact Person
Name
Mr. sc. Mario Kopljar
Email
[email protected]
Function
Surgery
Phone
+385 91 582 7446
Fax
+385 1 290 3624
Website
www.kbd.hr
Research Team Objectives
Main Fields
Surgery
Public health services
Social medicine
Disease itself, as well as surgical treatment of disease significantly changes the quantity and quality
of life. However, the influence of various diseases and surgical treatment options on the quality of life
remains insufficiently known, especially the impact on three dimensions of the quality of life: physical,
social and psychological health. Analysis of retrieved data will enable the impact of various diseases
and therapeutic options on all three dimensions of the quality of life, not only the quantity of physical
health, and therefore improve the treatment and speed up the recovery. The question of quality of life
is getting more attention in clinical research, as well as in everyday clinical practice. This is especially
related to investigations in surgery, where the number of published papers in this field has increased
20-fold in the last twenty years. The goal of the research of the quality of life is primarily
introductions mechanisms and standards for verifications of success of surgical treatment, and also
definition of those dimensions of quality of life that is most influenced by surgical therapy, in order to
enable targeted interventions for their improvement. Also, the analysis of the answers of treated
patients on standard quality of life questionaires will be used to develop new questionaires, specially
adjusted for particular subgroups of patients. The purpose of this research is improving the quality of
health care, especially in the field of surgery. Monitoring quality of life of operated patients during
treatment and in postoperative period will enable timely recognition of those patients that will benefit
most from changing therapeutic procedures, resulting in afirmation of new procedures and methods
of surgical treatment in addition to improving those already present. The results of this research will
be used for improving the quality of surgical treatment of patients. Disease itself, as well as surgical
treatment of the disease substantially changes the quantity and quality of health. Today, however,
the influence of various diseases and treatment options on the quality of life remains vague,
especially the effect on the three major constinuents of health: physical, social and psychological
health. Analysis of retrieved data will enable determination of the influence of various diseases and
therapeutic procedures on all three dimensions of the quality of life, and not only on the quantity of
physical health, and therefore improve the treatment process and fasten the recovery of patients
after surgery and reintegration in normal life in the community.
Partners/Interests
researcher
Surgery
Surgery
Institution/University
Name of Institution/University
University Hospital Dubrava
Number of Employees
n.a.
Number of Researchers
n.a.
Country
Croatia
Postal Adress
Av. G. Suska 6
10000 Zagreb
Surgery
Research Team
Research Team Name
Department of abdominal surgery, Clinical Hospital Dubrava,
Zagreb
Individual Researcher
No
Research Team Leader Name
Professor, MD, PhD Igor Stipancic
Research Team Members
Team Member 0
Professor Fiorella Biasi []
Team Member 1
MD, B.Sc. Gordana Kaic [[email protected]]
Team Member 2
MD, PhD Dusko Kardum [[email protected]]
Team Member 3
MD, PhD Andelko Korusic [[email protected]]
Team Member 4
MD Milka Ozegovic []
Team Member 5
Sci. ad., PhD Marija Poljak - Blazi [[email protected]]
Team Member 6
As. Prof. MD, PhD Zeljko Busic [[email protected]]
Team Member 7
MD, PhD Drazen Servis [[email protected]]
Team Member 8
MD Valentina Ratkajec [[email protected]]
Research Team Projects
Project Name
Surgical and oxidative stress in colorectal malignancies
Project Leader
Professor, MD, PhD Igor Stipancic [[email protected]]
Project Funding Agency
Ministry of science, Republic of Croatia
Project Budget
15000 €
Project Start Date
2002-08-22
Project End Date
2006-05-31
Project Partners
Croatia / Clinical Hospital Dubrava/ Department of abdominal
surgery Croatia / Clinical Hospital Dubrava/ Surgical biomedical
research lab Croatia / Ruder Boskovic Institute/ Department for
Molecular Medicine Oxidative Croatia / Ruder Boskovic
Institute/Laboratory for Oxidative Stress Austria / Institute fir
Molecular Medicine, Biochemistry and Microbiology in Graz Italy /
Institute for Biological and Medical Research in Torino
Project Summary
Cancer is an illness of continuos oxidative stress, while surgery represents acute local and systemic
oxidative stress. Thus, beside is direct medical usefulness, surgery induces stress response that might
promote or inhibit growth of possibly remaining cancer cells. This involves plays essential role also
inβactivity of cytokines among which TGF colon carcinogenesis. Hence, interference of surgical stress
involving cytokine network and activity of HNE, "second messenger of free radicals", would be of high
importance for the outcome. Project evaluated basic and clinical aspects of surgery in colon
malignancies that might improve monitoring patients' recovery and/or possible recurrence of disease
and reflect on additional therapies. Surgical injury is associated with increased production of reactive
oxygen species and utilization of antioxidant defense systems. Many experimental data are in support
of a damaging role of excess of oxidative reactions, also termed oxidative stress, in systemic stress
responses after surgical injury. Our findings obtained in previous project "Markers of systemic surgical
stress response" have shown that even laparotomic cholecistectomy can induce systemic stress
response that involves oxidative stress as well. Moreover, we have found that in rats surgical
oxidative stress differs between healthy and tumor bearing animals, in particular in the presence of
HO˙ radicals in blood . During oxidative stress lipid peroxidation occurs which leads to production of
reactive aldehydes, among which 4-hydroxynoneal is denoted as “second messenger of free radicals”
which also acts as a growth modifying factor for various types of cells including human carcinoma
cells . Growth modifying effects of HNE seem to be mediated through c-fos activity and interfere with
the effects of cytokines. Thus, HNE acts as a bifunctional growth factor that can stimulate growth of
cells, but also suppress it or even induce programmed cell death (apoptosis) . Among cytokines
relevant for the effects of HNE is TGF ß1, in particular for colon carcinomas. The TGF-beta´s are a
family of cytokines with antiproliferative activity on many cell types. TGFß1 can stimulate proliferation
of mesenchymal cells while stimulating the death of certain epithelial cells. This cytokine plays an
important role in the colon epithelium, not only in the inhibition of cell proliferation, but also in the
regulation of apoptosis. Escape from TGFß1-induced inhibition of proliferation has been observed in
Surgery
many tumor cells and may contribute to loss of growth control. In adenocarcinoma biopsies,
susceptibility to lipid peroxidation processes and TGFß1 expression are below the control. Similarly,
the adducts of HNE with proteins were significantly lower than controls in the plasma from cancer
patients and significantly higher in the plasma from patients with Crohn‘s disease, often regarded to
as precancerous. These findings suggest the existence of an association between oxidative damage
and TGFß1 expression in human intestine, both in case of inflammation and in carcinogenesis. While
monoclonal antibodies specific for HNE revealed the presence of HNE-adducts mostly in tumor
stroma, we also found that TGFß1 inhibits the growth of human CaCo-2 and HT-29 colon cancer cells,
and this cytokine’s action appears to be affected by HNE. Finally, our preliminary findings show that
TGFß1 causes apoptosis in CaCo-2 cells. We evaluated growth features of cultured human
adenocarcinoma and benign tumors (primary cultures) in respect to their sensitivity to HNE and
TGFß1 on one side, and on another monitor parameters of systemic oxidative status in operated
patients to define parameters suitable for improved monitoring of their stress response and
convalescence.
Project Website
www.mzos.hr
Project Name
The role of neutrophils and oxidative stress in colorectal
surgery
Project Leader
Professor, MD, PhD Igor Stipancic [[email protected]]
Project Funding Agency
Ministry of science, Republic of Croatia
Project Budget
10000 €
Project Start Date
2007-01-02
Project End Date
2011-01-02
Project Partners
Croatia / Clinical Hospital Dubrava/ Department of abdominal
surgery Croatia / Clinical Hospital Dubrava/ Surgical biomedical
research lab Croatia / Ruder Boskovic Institute/ Department for
Molecular Medicine Oxidative Croatia / Ruder Boskovic
Institute/Laboratory for Oxidative Stress Italy / Institute for
Biological and Medical Research in Torino
Project Summary
Surgical treatments induce local and systemic changes that involve neuroendocrine and the immune
system functions. Such surgical stress is associated with oxidative stress. Progress in minimal
invasive surgery, in particular laparoscopic surgery, is supported by clinical advantages in comparison
to open surgery and by assumption of lower intensity of surgical stress in thus operated patients. In
previous studies we described that even moderate surgical treatments, such as cholecystectomy, may
induce generalized, i.e. systemic stress response. This includes oxidative stress, which is less
pronounced in case of laparoscopy. It is certain that surgical removal of colorectal carcinomas causes
more severe stress for patients, not only because of the higher extent of surgery but also because
cancer itself causes oxidative stress. Inflammation, which accumulates iron, may have important
impact on the iron metabolism in these patients in particular in case of metastases. It remains to be
evaluated whether inflammation represents epiphenomenon of malignancy or a factor of cancer
progression, in particular because iron acts as pro-oxidant, which can together with reactive oxygen
species produced by inflammatory cells cause oxidative stress. Hence, controlling inflammation could
influence tumor development. Because neutrophils are the most abundant immune cells involved in
inflammation that produce mediators of oxidative stress (products of lipid peroxidation) thus
influencing the growth of other cells and inducing their death, we will study their effects in respect to
the type of surgical treatment of patients with colorectal carcinoma. Our current preclinical studies
indicate that neutrophils have anti-cancer effects in the early stage of tumor development based on
their oxidative burst and consequential oxidative stress. If in patients with colorectal carcinoma
functional activity of inflammatory cells is altered before surgery due to cancer caused immune
suppression, while surgery is followed by further change of the overall immune reactivity, it is an
open question how do we influence inflammatory response to cancer (functional activity of
neutrophils) in patients operated for colorectal carcinomas by open or by laparoscopic surgery? Our
hypothesis is that this is important factor that influences recovery of patients, disease progression
and the overall outcome.
Project Website
www.mzos.hr
Contact Person
Name
Professor Igor Stipancic
Email
[email protected]
Function
Abdominal surgeon
Surgery
Phone
+3851 290 3612
Fax
+2851 290 3626
Website
www.kbd.hr
Research Team Objectives
Main Fields
Surgery
Biochemistry
Other allied sciences
laparoscopic colorectal surgery; minimal invasive surgery; colorectal cancer; surgical stress; oxidative
stress; inflammatory response; granulocytes
Partners/Interests
researcher
industrial partner
Surgery
Biochemistry
Other allied sciences
Academic partners willing to collaborate in a regional and subregional international research project in
the field of colorectal cancer. We look for researchers which field of work is chemiluminiscence, iron
metabolism, oxidative stress, tumor cell lines, cytology and immunohistochemistry.
We plan to include colorectal cancer patients in this project. Only the patients that voluntarily sign
their consent, after being thoroughly informed about the project, will be included. After standardized
preoperative preparation, the patients will undergo surgery. The type of colon resection will be
primarily determined depending on cancer localization and stage, in order to fully meet the oncologic
principles of the resection. If oncologically possible, the patients will choose between laparoscopic or
open colon resection. All the patients will be operated by the project leader, to minimize treatment
variability. The operation, as well as anesthesiological procedures, will be maximally standardized.
The length of operation will be recorded, and later correlated with other findings. After the operation,
postoperative care will be carried out in intensive care unit, if necessary. During postoperative
hospital stay, all non-standard event, such as wound infection, elevated body temperature or other
signs of systemic infection, postoperative bleeding or anastomosis dehiscence, will be recorded, and
later correlated with other results. We will collect blood samples for every patient at 4 times:
preoperatively, 24 hours, 7 days and 28 days after the operation. Blood samples will be collected
from a peripheral vein, using Vacutainer system (Becton Dickinson, Plymouth, U.K.). Two vials
containing EDTA will be collected for blood cell analysis, and two vials without anticoagulant will be
collected for serum analysis. The collected blood will be used for measuring: complete blood count,
neutrophil / lymphocyte ratio, standard biochemical parameters (serum protein and albumin level,
liver transaminases, CRP, transferin, ferritin, bilirubin and other) using routine tests. Peripheral blood
neutrophil activity will be measured using chemiluminescence. From the peripheral blood,
lymphocytes will be separated by spinning blood samples in density gradient. They will be labeled
using monoclonal antibodies and analyzed using flow cytometer, in order to determine CD4+ / CD8+
T cell ratio. Finally, serum will be separated from blood samples, aliquoted in 5 plastic vials and
stored at –80°C. From these samples, we will later determine oxidative stress parameters using
photochemical and immunochemical methods at Rudjer Boskovic Institute, and serum IL-8 and IL-17
levels, using ELISA tests. After the operation, resected part of colon will be fixed in paraphine blocks
and analyzed at our Department for pathology. There, cancer stage will be determined according to
Dukes and TNM classification, as well as cancer infiltration of neutrophils and lymphocytes. Part of the
resected specimen will be immunohistochemically analyzed for IL-8 expression in cancer cells and
oxidative stress mediators. The collected data will be analyzed, and we hope to determine the
influence of colorectal cancer on neutrophil function, prognostic value of neutrophil infiltration of
cancer, and the influence of laparoscopic and open colon resection on postoperative recovery of
neutrophil function. We will analyze patients who developed postoperative complications, in order to
determine possible parameters that would reflect the severity of postoperative complications, or even
serve as early markers for postoperative complications, before the onset of clinical symptoms.
Institution/University
Name of Institution/University
Clinical Hospital Dubrava
Number of Employees
1600
Number of Researchers
600
Country
Croatia
Postal Adress
Avenija Gojka Suska 6
10000 Zagreb
Surgery
Research Team
Research Team Name
Department of Neurosurgery, School of Medicine, Zagreb
Individual Researcher
No
Research Team Leader Name
Professor, MD, PhD Josip Paladino
Research Team Members
Team Member 0
sci.as., MD Dinko Mihaljeviæ [[email protected]]
Team Member 1
psychologist, MSc Valerija Hauptfeld [[email protected]]
Team Member 2
psychologist, PhD Ljiljana Paèiæ-Turk [[email protected]]
Team Member 3
MD, PhD Ante Sekuliæ [[email protected]]
Team Member 4
prim., MD Ante Melada [[email protected]]
Team Member 5
As., MD, MSc Goran Mrak [[email protected]]
Team Member 6
As.prof., MD, PhD Miroslav Vukiæ [[email protected]]
Team Member 7
As.prof., MD, PhD Darko Chudy [[email protected]]
Team Member 8
Professor, MD, PhD Pavle Mikliæ [[email protected]]
Team Member 9
Prof., MD, PhD Josip Paladino [[email protected]]
Team Member 10
sci.as., MD Hrvoje Jednaèak [[email protected]]
Research Team Projects
Project Name
Functional neurosurgery
Project Leader
Professor, MD, PhD Josip Paladino [[email protected]]
Project Funding Agency
Ministry of Science, Republic of Croatia
Project Budget
5000 €
Project Start Date
2006-06-01
Project End Date
2011-06-01
Project Partners
Croatian Institute for Brain Research, Zagreb Department of
Neurology, School of Medicine, Zagreb Department of
Radiology,School of Medicine, Zagreb
Project Summary
Project Website
www.kbc-zagreb.hr
Contact Person
Name
Professor, MD, PhD Josip Paladino
Email
[email protected]
Function
Chief of Department of Neurosurgery, School of Medicine, Zagreb
Phone
+38512363530
Fax
+38512363531
Website
www.kbc-zagreb.hr
Research Team Objectives
Main Fields
Surgery
minimally invasive neurosurgery, cerebrovascular neurosurgery, endoscopic neurosurgery, skull base
surgery, spinal neurosurgery, radiosurgery (Gamma knife), functional morphology of CNS, surgery of
congenital disorders, neurotraumatology
Partners/Interests
researcher
Surgery
Institution/University
Name of Institution/University
University of Zagreb
Number of Employees
7817
Surgery
Number of Researchers
1108
Country
Croatia
Postal Adress
Trg Maršala Tita 14
10000 Zagreb
Surgery
Research Team
Research Team Name
Maxilofacial Surgery
Individual Researcher
No
Research Team Leader Name
Mr. MD, PhD, Danijel Žerdoner
Research Team Members
Team Member 0
Mr. MD, PhD Matjaž Rode []
Contact Person
Name
Mr. MD, PhD, Danijel Žerdoner
Email
[email protected]
Function
Assistant Professor of Maxilofacial Surgery
Phone
+386 3 423 3000
Fax
+ 386 3 423 36 66
Website
http://www.sb-celje.si/index.php?id=15
Research Team Objectives
Main Fields
Surgery
Maxilofacial Surgery
Partners/Interests
researcher
industrial partner
Suitable
Surgery
1. IMAGE CYTOMETRIC EVALUATION ON SMEARS OF ORAL MUCOSA: A POSSIBLE APPROACH FOR
THE EARLY DETECTION OF ORAL CANCERS AND PRECANCERS Cancer is one of the major threats to
public health. In developed countries cancer is the second most common cause of death. It is
noteworthy that sharp increases in the incidence rates of oral and pharyngeal cancers have been
reported for several EU countries. Despite the progress of modern medicine in the treatment of
various cancers, the prognosis of patients with oral cancers is continuously poor. One reason for this
is that the diagnosis usually is achieved late. These tumors are generally slow growing and may not
arouse suspicion until they ulcerate, become secondarily infected, or invade adjacent structures. Up
to 80% oral cancers are not diagnosed on early stage. Therefore, there is an need for new strategies
to detect these cancers early. Currently there is intensive research in the development of molecular
biomarker assays. Alternatively new techniques of cytoanalysis could be used. One of these
approaches is the assessment of structural changes in cell nuclei that derive from the neighborhood
of premalignant or malignant lesions. This phenomenon is known as malignancy- associated changes
in the DNA organisation. The aim of the study is to investigate the possibility of identifying oral
cancers and precancers by nuclear chromatin texture feature analysis of cell nuclei from mucosal
scrapings obtained from clinically alternated oral mucosa by high-resolution image cytometry.
Institution/University
Name of Institution/University
General Hospital Celje
Number of Employees
1613
Number of Researchers
37
Country
Slovenia
Postal Adress
Oblakova 5
3000 Celje
Surgery
Research Team
Research Team Name
Surgery
Individual Researcher
Yes
Research Team Leader Name
Mr. MD, PhD Eldar M. Gadžijev
Contact Person
Name
Mr. MD, PhD Eldar M. Gadžijev
Email
[email protected]
Function
Professor of Surgery
Phone
+386 2 321 12 33
Fax
+ 386 2 331 23 93
Website
http://www.sb-mb.si/index.php?id=kirurska-sluzba
Research Team Objectives
Main Fields
Surgery
Partners/Interests
researcher
industrial partner
Suitable
Surgery
1.ANATOMICAL VARIATIONS OF THE HOLLOW LIVER STRUCTURES AND THEIR SURGICAL
IMPORTANCE After separate studies of the hepatic veins, portal vein system, hepatic artery system
and biliary system performed on corrosive casts of the cadaver livers congruity and variations in the
courses of the elements of portal triad are the matter of research interest in further study.
Differences in the course and branches comparing portal vein system and biliary system on the left
liver have already been studied, but their presence, frequency and importance inside the right liver
have to be searched. When such variations are present they can have important impact on
complications and some postoperative morbidity after liver resections. Results of studies should be
confirmed using 3D CT pictures in patient cases. Another segment of this study is hepatic venovenous anastomoses which could convert blow flow from the region of one hepatic vein into the
region of another after ligating the first one. 2. ABDOMINAL COMPARTMENT SYNDROME AND
MEASUREMENTS PROBLEMS Our studies of reproducible liver injury resulted in evaluation of a
successful system to achieve it on experimental animal. Precisely labeled cross bow shots using
arrows with a ball at the end has given us a possibility to perform a reproducible liver injury. Further
problem to be introduced has been evaluation of the pressure of liver packing and specificity of
abdominal compartment syndrome. Measurement of the pressure of liver packing is not to be
measured like other compartment syndromes. 3. SYMBIOTIC IN PREVENTION OF INFLAMMATORY
RESPONSE AFTER MAYOR SURGERY Study of symbiotic support in diminishing inflammatory response
after mayor surgery is already going on. Further research is planned to be focused on replacement of
antibiotic prophylaxis by symbiotic, leaving antibiotics for treatment when needed. 4. PREVENTION
OF BILE DUCT INJURIES DURING LAPAROSCOPIC CHOLECYSTECTOMY Epidemiologic analysis of the
bile duct injuries in our country has been recently performed. A study is planned introducing
measures with steps and manners during laparoscopic cholecystectomy that should prevent or
diminish bile duct injuries.
Institution/University
Name of Institution/University
University of Maribor - Medical faculty
Number of Employees
30
Number of Researchers
15
Country
Slovenia
Postal Adress
Slomskov trg 15
2000 Maribor
Therapeutics
Research Team
Research Team Name
Centre for Study the Human Motricity-University of Craiova,
FEFS
Individual Researcher
No
Research Team Leader Name
Prof.dr. RUSU LIGIA
Research Team Members
Team Member 0
Prof.dr. Stroe Corneliu Andrei [[email protected]]
Team Member 1
Prof.dr. Dragomir Marcela [[email protected]]
Team Member 2
Prof.dr. Cataneanu Sergiu [[email protected]]
Team Member 3
Prof.dr. Dragomir Marian [[email protected]]
Team Member 4
Prof.dr. Ortanescu Dorina [[email protected]]
Team Member 5
Prof.dr. Avramescu Taina Elena [[email protected]]
Team Member 6
Prof.dr. Ortanescu Corneliu [[email protected]]
Team Member 7
Prof.dr. Danoiu Mircea [[email protected]]
Team Member 8
Prof.dr. Gusti Alice [[email protected]]
Team Member 9
Asist. prof.dr. Calinescu Gheorghe [[email protected]]
Team Member 10
Prof.dr. Rusu Ligia [[email protected]]
Team Member 11
Lecturer Calina Mirela [[email protected]]
Team Member 12
Lecturer Vasilescu Mirela [[email protected]]
Team Member 13
Lecturer Rosulescu Eugenia [[email protected]]
Team Member 14
Lecturer Cernaianu Sorina [[email protected]]
Team Member 15
Lecturer Ilinca Ilona [[email protected]]
Team Member 16
Asist. Dumitrescu Aurora [[email protected]]
Team Member 17
Asist Nanu Costi [[email protected]]
Team Member 18
asist. Zavaleanu Mihaela [[email protected]]
Team Member 19
asist. Munteanu Germina [[email protected]]
Team Member 20
asist. Calinescu Luminita [[email protected]]
Team Member 21
asist. Enescu Denisa [[email protected]]
Team Member 22
lecturer Barbu Mihai Razvan [[email protected]]
Team Member 23
lecturer Ciocanescu Daniel [[email protected]]
Research Team Projects
Project Name
Aquisitions of new skills and practical knowledge for
students in kinetotherapy
Project Leader
Prof.dr. Rinderu Avramescu Taina Elena [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2002-10-01
Project End Date
2003-10-01
Project Partners
University of Craiova, Romania Clinical Hospital Nr. 1 Craiova,
Romania Neurology Hospital Nr. 4 Craiova, Romania Alfa and
Omega Tourisme Agency, Craiova , Romania Hogeschool West
Vlaanderen Kortrjik, Belgium Cnetre for development of human
resources and vocational training, Pyxdia, Greece Totnes European
School, Devon, UK
Project Summary
In this project we promote the role of education and new aquisitions skills of students at
kinetotherapy for increase their theoretical and practical prepare regards the last european standards.
Traget group-13 students and kinetotherapy that go to educational institutions in Europe for 3
months.
Therapeutics
Project Website
http//cis01.central.ucv.ro/en/educatie_fizica
Project Name
Training Centre for Health Care, Prophylactic and
Rehabilitation Services
Project Leader
Prof.dr. Rinderu Avramescu Taina Elena [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2005-10-01
Project End Date
2007-03-01
Project Partners
University of Craiova,Romania Prefecture of Dolj, Romania
University Foundation of Kinetotherapy, Oradea, Romania
University of Oradea, Romania Hogeschool West Vaanderlenden
Kortrjik Belgium Entente,UK SCUE/Studio di consulenza per
l"Unione Europea , Italy Technical University of Crete, Greece
Project Summary
Deveoplment the reserach centre for rehabilitation the disabilities perssons, using the prohylactic
services and alsostudents participation to development the research activity regards the role of
physical activity in rehabilitation. Change from our lecturer and specialists with other specialists form
European country.
Project Website
http//cis01.central.ucv.ro/en/educatie_fizica
Project Name
Alghoritm for implementation the physical activity in
rehabilitation of neuromuscular disorders
Project Leader
Prof.dr. Rusu Ligia [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-03-01
Project End Date
2007-03-01
Project Partners
University of Craiova, Romania Nuerologyc Hospital Craiova,
Romania
Project Summary
It is a national programme for research, that are organise by CNCSIS and Ministery of Education in
Romania. The project research the modalities for introduce the physical activity in rehabilitation
programme for patients with neuromuscular disorders, and create some assesment scale for prevent
thedecrease the health status of these patients and for prevent the muscle weakness and fatigue.
Project Website
http//cis01.central.ucv.ro/en/educatie_fizica
Contact Person
Name
Prof.dr. RUSU LIGIA
Email
[email protected]
Function
Director of Research Centre
Phone
0040251563755
Fax
0040251422090
Website
www.ucv.central.ro
Research Team Objectives
Main Fields
Therapeutics
Physiology
Social medicine
Main fields of our centre is to study the numan body motricity and performance, the role of physical
effort in increase and improvement the health status. We have also study regards the implication of
physical activity in rehabilitation of patients with multiple sclerosis. We made study regards
physiologycal and morphologycal aspects that are the results of physical activity in neuromuscular
disorders and osteoporosis and also fpr prevent the ageing process. We are preocupate for
development the research related kinetic analyse of body movement and how we can increase the
apport of these results in rehabilitation and prevention programme.
Therapeutics
Partners/Interests
researcher
industrial partner
Biophysics
Physiology
Therapeutics
Experience in research of human body, physiology, rehabilitation, morphophysiology in
neuromusuclar disorders, osteoporosis and ageing.
We want to devlopment the research regards movement analyse of human body and the implication
of physical activity in rehabilitation of perssons with chronic disease for increase the health statuts
and so to reduce the social costs. We want to development the study regards ageing and how the
physical activity can improve the life at elderly people. Development the reserch in multiple sclerosis
and the role of physical activity in change the morphophysiologyc aspects in multiple sclerosis.
Institution/University
Name of Institution/University
University of Craiova, Physical Education and Kinetotherapy
Number of Employees
1000
Number of Researchers
150
Country
Romania
Postal Adress
A.I.Cuza 13
1100 Craiova
Therapeutics
Research Team
Research Team Name
Department of Oncology and Nuclear Medicine, Sisters of
Charity University Hospital, Zagreb, Croatia
Individual Researcher
No
Research Team Leader Name
Professor Josip Lukac
Research Team Projects
Project Name
RT-PCR determination of circulating tumor cells in patients
with solid tumors
Project Leader
Prof Josip Lukac [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2006-01-01
Project End Date
2011-12-31
Project Partners
Project Summary
Metastases are the major cause of cancer-related deaths in patients with solid epithelial tumors.
Hematogenous spreading of tumor cells is considered as a crucial step in tumor dissemination.
Therefore, the detection of disseminated tumor cells in peripheral blood might be of clinical
significance with respect to individual patient prognosis and monitoring of therapy. Reverse
transcriptase-polamerase chain reaction (RT-PCR) assay allows determination of molecular tumor
markers expressed in disseminated tumor cells, thus providing a powerfull tool for detection of
disseminated tumor cells with high sensititity and specificity. Colorectal cancer (CRC) is the third
cause of death of cancer patients in EU. Although systematic colonoscopic examinations gratly
contributed to early diagnosis of CRC and allowed earlier surgical interventions, substantial proportion
of patients die from recurrences and/or distant metastases. More reliable prognostic factors would
allow more accurate identification of these increased-risk subgroups. Based on its restricted
expression pattern, cytokeratin 20 (ck20) has been mainly used as a tumor marker to detect tumor
cells in the peripheral blood of patients with CRC but the results regarding its specificity and clinical
usefulness are still conflicting. Some studies showed that ck20 is a reliable marker, not detectable in
the blood of noncancer patients, while others detected it in a proporton of patients with non-tumor
disease so that its sensitivity/specificitiy and the usefulness as a prognostic marker still needs to be
evaluated. Also the evidence about the usefulness of detection of tumor cells in mesenteric blood
taken intraoperatively, as a more reliable predictor of liver metastases is scarce and should also be
evaluated as should be the detection of tumor cells in peripheral blood taken intraoperatively, as a
predictor of tumor disssemination mediated by the surgical procedure itself. Although not among
tumors with the highest incidence, malignant melanoma represents a huge healthcare problem
because its incidence is significantly increasing in the last few decades and because there is still no
available treatment option that would significantly prolong the survival of patients with advancedstage disease. Therefore, it is important to find biological markers which would be predictive
particularly for spreading of metastases and could help to improve the management of patients. The
detection of circulating melanoma cells by RT-PCR is being investigated as a potential prognostic
marker and marker for monitoring response to therapy. The most widely used melanoma-specific
marker in RT-PCR detection of circulating melanoma cells is the expression of tyrosinase gene (Tyr).
The major limitation in clinical use of tyrosinase as a marker is a relatively high number of melanoma
patients with clinically confirmed distant metastases being tyrosinase negative. It has been shown
that determination of Melan-A/MART-1 in addition to tyrosinase enables the RT-PCR detection of
circulating melanoma cells in a larger percentage of melanoma patients. We were first to show that
analysis of microphthalmia-associated transcription factor (MiTF) as an additional marker to
tyrosinase enables the detection of circulating melanoma cells in a larger percentage of melanoma
patients. Therefore, the usefulness of multiple marker detection in increasing of sensitivity needs to
be further elucidated. Although much progress has been made in the treatment of differentiated
thyroid cancer, disease recurrence and metastases may still occur in 20 – 40 % of patients. Since
most of the recurrences can be managed successfully by surgical and radioiodine treatment it is
important to monitor patients after thyroidectomy to detect a recurrence as early as possible.
Measurement of serum thyroglobulin (Tg) protein by immunoassay is commonly used highly specific
test for detecting recurrent or residual thyroid cancer. A major limitation of this test is a presence of
anti-Tg antibodies in 10-20 % of patients, which interferes with serum Tg measurement. There has
therefore been an interest in developing new sensitive assays for thyroid cancer detection that are
not influenced by presence of anti-Tg antibodies. One such approach is detection of circulating thyroid
Therapeutics
cancer cells by RT-PCR. The most widely studied thyroid-specific marker in RT-PCR detection of
circulating thyroid cancer cells is the expression of Tg gene. The prognostic value of circulating
thyroid cancer cells detection by RT-PCR hasn’t been studied so far. We would like to study the
prognostic value of determination of circulating tumor cells by RT-PCR: (i) in peripheral blood prior to
surgical resection of primary tumor and intraoperatively, in mesenteric blood intraoperatively, in
peripheral blood at each follow-up visit for three post-operative years, and/or prior to and following
rasdiotherapy/chemotherapy in colorectal cancer patients (marker: cytokeratin 20); (ii) in peripheral
blood of melanoma patients prior to surgical resection of primary tumor and at each follow-up visit for
three post-operative years and/or prior to and following chemotherapy in those requireing further
treatment (markers: tyrosinase and microphthalmia transcription factor); and (iii) in peripheral blood
of patients with differentiated thyroid cancer at each follow-up visit throughout the first three years
following tumor resection (thyroglobulin as a marker).
Project Website
Contact Person
Name
Professor Josip Lukac
Email
[email protected]
Function
Head, Division of Laboratory Diagnostics
Phone
+3851 3787 228
Fax
+3851 3768 303
Website
www.kbsm.hr
Research Team Objectives
Main Fields
Therapeutics
Therapeutics
Immunology and Immunohaematology
Oncology; tumor markers; circulating tumor cells by RT-PCR; colorectal cancer; melanoma; thyroid
cancer
Partners/Interests
researcher
Therapeutics
Genetics
Immunology and Immunohaematology
Facilities and knowledge in performing PCR and RT-PCR
Applied/clinical research of the usefulness of determination of circulating tumor cells detected by RTPCR as a prognostic factor in patients with solid tumors (particularly colorectal cancer, melanoma,
thyroid cancer and breast cancer).
Institution/University
Name of Institution/University
Sisters of Mercy University Hospital
Number of Employees
2290
Number of Researchers
116
Country
Croatia
Postal Adress
Vinogradska 29
10000 Zagreb
Therapeutics
Research Team
Research Team Name
Ortopaedics
Individual Researcher
Yes
Research Team Leader Name
Mr. MD, PhD Samo Fokter
Contact Person
Name
Mr. MD, PhD Samo Fokter
Email
[email protected]
Function
researcher
Phone
+386 3 423 30 00
Fax
+ 386 3 423 36 66
Website
www.sb-celje.si
Research Team Objectives
Main Fields
Therapeutics
Ortopaedics
Partners/Interests
researcher
industrial partner
Suitable
Therapeutics
Use of bisphosphonates to improve the durability of total hip replacement Total joint replacements
are effective surgical procedures for treating patients with end-stage arthritis. However, this
procedure is associated with an incidence of failure that increases at the rate of approximately 1%
per year postoperatively. Failure is often due to aseptic loosening with bone loss surrounding the
implant. Particularly around total hip arthroplasties (THA), insertion of a stiff metal implant alters the
loading pattern of the surrounding bone. Body weight is transferred along the implant to the femoral
diaphisis, thereby bypassing the proximal femur (stress shielding). This removal of load results in
early periprosthetic bone loss (i.e. 3 to 6 months following THA), which may exceed 30% of the bone
mass as determined by dual-energy X-ray absorptiometry (DXA). If allowed to progress, implant
stability may be compromised resulting in complications such as implant destabilization and
periprosthetic bone fracture. Revision surgeries have a higher rate of local and systemic
complications, requiring longer hospital stays; they have less favorable outcomes, and are more
expensive than the initial surgery. Bisphosphonates effectively inhibit bone resorption and are the
pharmaceutical agents of choice in managing postmenopausal osteoporosis, hypercalcaemia, and
Paget’s disease. Bisphosphonates bind avidly to hydroxyapatite crystals and thus have a strong
affinity for bone mineral. During bone remodeling process, osteoclasts take up the bisphosphonate,
which inhibits the mevalonate pathway. Osteoclasts lose the ruffled border and are inactivated as a
result of apoptosis. Several studies have shown that bisphosphonates, administered systemically,
may be effective in preventing the bone loss associated with stress shielding. Since bisphosphonates
have some side effects like fever, throat or stomach ulcers as well as a low bioavailability, local
delivery with the implant coated with a bisphosphonate would be highly appreciated. The bone in
contact with the implant will be the only part of the skeleton being exposed to the drug. This is
important as patients undergoing THA do not necessarily present osteoporosis. We aim at
determining whether a bisphosphonate locally released from an implant could decrease the
periprosthetic bone loss after THA. The study design will be prospective, randomized, and blind.
Periprosthetic bone mineral density (BMD) will be measured with DXA at total periprosthetic area as
well as at seven Gruen zones or regions of interest. DXA scans will be performed at one week
(baseline), six weeks, three months, six months, and 12 months postoperatively. Mean values of the
demographics, BMD, and baseline-normalized BMD values will be compared between the
bisphosphonate and placebo groups at each time point. For all temporal measurements including BMD
and normalized BMD, mean values will be compared across each time point within a given group. In
that manner, it would be possible to show if bisphosphonate covering of the endoprosthesis could
potentially increase the longevity of the THA and thus decrease the necessity for revisions, reduce
patient suffering, and lessen the costs for the society.
Institution/University
Name of Institution/University
General Hospital Celje
Number of Employees
1613
Number of Researchers
37
Therapeutics
Country
Slovenia
Postal Adress
Oblakova 5
3000 Celje
Therapeutics
Research Team
Research Team Name
Individual Researcher
Yes
Research Team Leader Name
Prof. dr. Meliha Lekić PhD
Contact Person
Name
Prof. dr. Meliha Lekić PhD
Email
[email protected]
Function
Head of the Department of Haematology
Phone
+33 663 742 / 743
Fax
+33 203 670
Website
www.mf.unsa.ba
Research Team Objectives
Main Fields
Therapeutics
Partners/Interests
AN EVALUATION OF OXIDOREDUCTION PROCESSES OF FITTED ORTHOPEDIC IMPLANTS
MADE OF ALLOYED STAINLESS STEEL BY MONITORING THE LEVEL OF ALLOY ELEMENTS IN
BODY FLUIDS
The choice of materials for orthopedic implants depends on their mechanical and chemical
biocompatibility. Biological environment (body fluids) with high concentration of chloride ions and
various complexifying agents stimulate the decomposition of the material, releasing metallic ions in
the surrounding tissues. The ions are transported through the body: they link with proteins, enter the
cells of surrounding tissues and stay at the implant’s place, or they are reduced and become inert.
These processes are exceedingly interesting from the medical point of view, since the research of the
impact of various oxidizing metal conditions helps to explain some illnesses occurring in patients with
fitted orthopedic implants.
Stainless steel is the most susceptible to oxidoreduction processes among orthopedic materials. If
the concentrations of alloy elements exceed normal values, all three main elements – iron, chrome
and nickel – are potential carcinogens for humans. It is well known that nickel can cause toxic and
allergic reactions, resulting in cell destruction. Chrome, which is the highest percentage alloy element
in these alloys (17 to 19%), shows genotoxic characteristics in the form of Cr6+ ion. The stainless
steel implant’s degradation manifests in the higher concentration of iron, chrome and nickel in urine,
lungs, liver, kidneys and spleen. These values can even exceed normal ones ten times.
Hypothesis
Monitoring the physicochemical metal implants corrosion parameters in vitro correlated with
determination of implant’s alloy elements concentration in vivo, it is possible to predict how long
particular metal implants can stay in the body before causing significant disturbances triggered by
degradation processes of implants themselves.
Probably the results will depend from quality and quantity of implant materials in different countries
of South-East Europe.
Research aims
•
Monitor oxidoreductive processes of the fitted orthopedic implants by monitoring the level of
alloy elements of the implant in body fluids.
•
Define types of oxidoreductive processes on implants with various purposes.
•
Define oxidoreductive processes parameters on stainless steel implants.
•
Determine corrosion speed on stainless steel implants in vitro in simulated in vivo conditions.
Participants
Serum and 24 hours urine of patients will be analyzed according to a predetermined plan in
preset time intervals, from the implant’s fitting to its removal. The control group would consist of the
same participants whose blood and one-time urine would be taken immediately before fitting.
Methods applied
Atomic absorption spectrophotometry with graphite furnace cuvette will be used for determination
of alloy metals concentration in body fluids.
Chemical stability of implant materials will be tested in vitro, in simulated physiological conditions
(electrolyte solution, pH, temperature, pressure) by application of electrochemical methods using
potentiostatic and galvanostatic techniques.
Results expected
Obtained results of oxidoreductive processes in implantation materials research could depend from
Therapeutics
quality and quantity of that materials and can be important for orthopedists and casualty surgeons.
Based on them it will be possible to determine how long a certain metal implant can stay in the
body to achieve the full osteosynthetic effect without causing any important risk of possible
intoxication by ions of alloy elements.
Institution/University
Name of Institution/University
University of Sarajevo, Faculty of Medicine Sarajevo
Number of Employees
Number of Researchers
Country
Bosnia and Herzegovina
Postal Adress
Čekaluša 90
71000 Sarajevo
Therapeutics
Research Team
Research Team Name
Individual Researcher
No
Research Team Leader Name
Prof. Željiko Knez
Research Team Members
Team Member 0
Zoran Novak [[email protected]]
Team Member 1
M. Škerget
Contact Person
Name
Prof. Željiko Knez
Email
[email protected]
Function
Phone
+386 2 2294461
Fax
+386 2 2516750
Website
http://atom.uni-mb.si/labs/lab_sep/engindex.htm
Research Team Objectives
Main Fields
Therapeutics
Pharmacology
Partners/Interests
INOVATIVE PROCESSES FOR INOVATIVE DRUGS
Many drugs possess poor water solubility and their bioavailabilities are limited by their dissolution
rates. Several approaches are known to increase the dissolution rate and hence the bioavailability of
such drugs including micronization, formation of solid dispersions, solvates, adsorbates or complexes.
Technologies using supercritical fluids (SCFs) can also be used in this sense. Beside their extractive,
chromatographic and applications in biochemical reactions, SCFs offer interesting applications in
materials processing. In recent years, processing of pharmaceuticals with SCFs, especially with
supercritical carbon dioxide, has received increased attention. Supercritical micronization techniques
are: rapid expansion of a supercritical solution (RESS), gas anti-solvent recrystallisation (GASR), high
pressure crystallization (HPC) and particles from gas saturated solutions (PGSS). The PGSS method
shows some advantages over RESS, GASR and HPC processes. Compared to RESS the consumption of
CO2 is an important parameter being lower by an order of magnitude 103 for PGSS than for RESS.
Since in RESS the substance has to be dissolved in the supercritical gas, sufficient solubility in SC CO2
is the criterion which eliminates many pharmaceutical compounds from the RESS process. In PGSS on
the contrary, the compressible gas is dissolved in the molten compound and the gas saturated liquid
phase is further processed. When compared to GASR advantageously no organic solvent is needed in
the PGSS process.
With the PGSS process micronized drugs and drug composites can be prepared in different way,
which has some advantages over classical methods for micronization of pure drugs and for
drug/carrier solid dispersion preparation, namely fusion methods and solvent processes. In the PGSS
process there is a melt of the drug (and a carrier), saturated with supercritical CO2. Upon expansion
the gas evaporates and therefore this solution is rapidly cooled down and the drug (or drug/carrier)
precipitates in form of microparticles, thereby avoiding the comminution step. Since the cooling is
rapid due to the expansion of CO2 present fine particles with a narrow particle size distribution can be
formed. With the PGSS process, micronized drug or micronized drug/carrier can be obtained in one
step with no organic solvent. The condition for successful micronization with the PGSS process is
sufficient solubility of supercritical CO2 in the melt to achieve the expandable gas saturated solution.
Institution/University
Name of Institution/University
Faculty of Chemistry and Chemical Engineering, University of
Maribor
Number of Employees
Number of Researchers
Country
Slovenia
Postal Adress
Smetanova ul. 17
2000 Maribor
Therapeutics
Research Team
Research Team Name
Evidence-based medicine
Individual Researcher
No
Research Team Leader Name
Univ. Doz. Dr. Andrea Siebenhofer-Kroitzsch
Research Team Members
Team Member 0
OA Dr. Karl Horvath
Team Member 1
Dr. Klaus Jeitler
Contact Person
Name
Univ. Doz. Dr. Andrea Siebenhofer-Kroitzsch
Email
[email protected]
Function
head of the EBM Review Center
Phone
Fax
Website
http://www.meduni-graz.at
Research Team Objectives
Main Fields
Therapeutics
Evidence-based medicine
Partners/Interests
The central function of Review Centers ist the implementations of an systematic overview and metaanalyses of precisely defined medical items and problems. These subsequently represent the basis for
decisions regarding supply in health care, health technology assessments, disease management
programs or guidelines and serve as assistance för diagnostic and therapeutic decisions of attending
physicians.
Additional functions include the procurement of EBM-competences for attending physicians in form of
training courses as well as the further development of methods for the evaluation of surveys and
training of medical students.
Institution/University
Name of Institution/University
EBM-Review Center, Medical University Graz
Number of Employees
Number of Researchers
Country
Austria
Postal Adress
Auenbruggerplatz
8036 Graz
Toxicology
Research Team
Research Team Name
Laboratory of environmental and clinical analytical
toxicology and environmental health problems
Individual Researcher
No
Research Team Leader Name
PhD in analytical chemistry Alina Catrinel Ion
Research Team Members
Team Member 0
PhD student Anton Ficai [[email protected]]
Team Member 1
PhD student Cornelia Dragnea [[email protected]]
Team Member 2
professor Ana Banica [[email protected]]
Team Member 3
professor Ion Ion [[email protected]]
Team Member 4
professor Alina Ion [[email protected], [email protected]]
Contact Person
Name
PhD in analytical chemistry Alina Catrinel Ion
Email
[email protected]
Function
professor
Phone
0723295334
Fax
+440212319492
Website
www.acad.ro
Research Team Objectives
Main Fields
Toxicology
Social medicine
Clinical chemistry
environmental and environmental health problems; metal ion speciation and its applications in human
health; modern trends of metal ions research, speciation, quality, assurance and risk assesement
environmental pathology and exposure to metal ions; nutrition, metal ions and ageing
Partners/Interests
researcher
industrial partner
Toxicology
Information Technology, Statistics, Documentation
Clinical chemistry
researchers and industrial partners in the field of public health
Cu, Cd, Zn, Pb, Al speciation in environmental and clinical samples; study of their multiple effects
over human health
Institution/University
Name of Institution/University
University Politehnica of Bucharest
Number of Employees
5000
Number of Researchers
1600
Country
Romania
Postal Adress
Polizu street 1
78123 Bucharest
Toxicology
Research Team
Research Team Name
Medical faculty Foca University East sarajevo, Bosnia and
Hercegovina
Individual Researcher
No
Research Team Leader Name
associate professor Veljko Maric
Research Team Members
Team Member 0
associate professor, Veljko Maric dean, surgeon []
Team Member 1
assistant professor Stevan Trbojevic vicedean, gastroenterologist []
Team Member 2
professor Slobodan Milovanovic pharmacologist []
Team Member 3
assistant professor Sinisa Ristic physiologist, []
Team Member 4
assistan professor Milan Kulic genetics []
Research Team Projects
Project Name
Balcanic endemic nephroparhy in BiH
Project Leader
dean, associate professor Veljko Maric
[[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2007-01-01
Project End Date
2010-01-01
Project Partners
Project Summary
We should investigate epidemiological, clinical, biochemistry, genetical characteristic of patient with
BEN and their family member.
Project Website
Contact Person
Name
associate professor veljko maric
Email
[email protected]
Function
dean
Phone
0038758 210 420
Fax
0038758 210 007
Website
http://www.mf-foca.com
Research Team Objectives
Main Fields
Toxicology
Internal Medicine
Epidemiology
Balcanic endemic nephropathy in BiH (Biljeljina region)
Partners/Interests
Biology
Institution/University
Name of Institution/University
medical faculty
Number of Employees
70
Number of Researchers
20
Country
Bosnia and Herzegovina
Postal Adress
Solunskih dobrovoljaca 1
73300 Foca
Toxicology
Research Team
Research Team Name
Mineral Metabolism Unit
Individual Researcher
No
Research Team Leader Name
M.D., Ph.D. Martina Piasek
Research Team Members
Team Member 0
M.D., Ph.D., F.C.A. Krista Kostial-Šimonović [[email protected]]
Team Member 1
Ph.D. Maja Blanuša [[email protected]]
Team Member 2
B.Sc. Tatjana Orct [[email protected]]
Team Member 3
B.Sc. Maja Lazarus [[email protected]]
Team Member 4
M.D., Ph.D. Veda Marija Varnai [[email protected]]
Team Member 5
M.Sc. Dijana Jureša [[email protected]]
Team Member 6
Ph.D. Jasna Jurasović [[email protected]]
Team Member 7
M.D., Ph.D. Martina Piasek [[email protected]]
Research Team Projects
Project Name
Exposure, intake and effects of toxic and essential elements
Project Leader
Dr. Martina Piasek [[email protected]]
Project Funding Agency
Croatian Ministry Sci. Edu. Sports
Project Budget
170.000 €
Project Start Date
2002-07-24
Project End Date
2006-12-31
Project Partners
Investigators in Croatia, Institute for Medical Reasaech and
Occupational Health: Veda Marija Varnai Selma Cvijetiæ Avdagiæ
Dijana Jureša Tatjana Orct Maja Lazarus Maja Blanuša Krista Kostial
Collaborators from USA: Jasminka Z. Ilich Velimir Matkoviæ John
W. Laskey
Project Summary
Toxicokinetics of metals (cadmium, lead, mercury) and interactions of toxic and essential elements
(calcium, iron, zinc, copper, selenium); Reproductive and perinatal health effects of metals
(particularly cadmium) and assessment of bone health especially in young in man and animals;
Influence of age, nutrition, and chelation therapy on metal toxicokinetics and health effects in
experimental rats; Testing of novel chelating agents and dietary measures for reducing toxic metal
body burden in experimental rats. Biomonitoring and monitoring of metals and metalloids by analysis
of various elements in biological materials (human and animal), food, water, and soil by atomic
absorption spectrometry, flame and electrothermal AAS (As, Ca, Cd, Cu, Fe, Hg, Mn, P, Pb, Se, Zn).
Project Website
//bib.irb.hr/pregledi?chset=ASCII〈=EN
Project Name
Biomedical research on reproduction and development
(research on placenta and metal exposure)
Project Leader
Professor Davor Ježek [[email protected]]
Project Funding Agency
Project Budget
€
Project Start Date
2003-11-20
Project End Date
2006-12-31
Project Partners
Part of a national collaborative research project at Zagreb University Medical School
Project Website
Contact Person
Name
M.D., Ph.D. Martina Piasek
Email
[email protected]
Function
Scientific Adviser
Toxicology
Phone
++385 1 4673 188
Fax
++385 1 4673 303
Website
http://www.imi.hr/index.php?lang=en
Research Team Objectives
Main Fields
Toxicology
Pharmacology
Public health services
Evaluation of toxicokinetics and health effects of toxic and essential elements: Health effects of toxic
metals (cadmium, lead, mercury, aluminium) and interactions of toxic and essential elements
(calcium, iron, zinc, copper, selenium), particularly reproductive and perinatal effects and bone
health; Influence of age, sex, nutrition, and chelation therapy on metal toxicokinetics and health
effects in experimental rats; Testing of novel chelating agents and dietary measures for reducing
toxic metal body burden in experimental rats. Monitoring and assessing environmental exposures to
metals and metalloids by analysis of various elements in biological materials (human and animal),
food, water, and soil by atomic absorption spectrometry (flame and electrothermal AAS): Ag, Al, As,
Ca, Cd, Cu, Fe, Hg, Mn, P, Pb, Pt, Se, Zn.
Partners/Interests
researcher
Toxicology
Clinical chemistry
Public health services
Experiance in steroid hormone analysis to assay placental tissue steroid hormones.
Assesment of exposure and effects of metals as endccrine disupting chemicals by placental metal
concentrations and placental steroidogenesis in human and experimental rat.
Institution/University
Name of Institution/University
Institute for Medical Research and Occupational Health, Zagreb
Number of Employees
147
Number of Researchers
84
Country
Croatia
Postal Adress
Ksaverska cesta 2
10001 Zagreb