C HRONIC PELVIC

In vivo
CHRONIC PELVIC PAIN INDUCED BY CARRAGEENAN
A MODEL FOR CHRONIC PROSTATITIS / CHRONIC PELVIC PAIN SYNDROME (CP/CPPS)
MODEL
Intraprostatic injection of carrageenan
induces pelvic pain
Effects on the nociceptive threshold (A), the nociceptive
scores (B) and the AUC (C), 3 days post-injection.
A
Nociceptive threshold (g)
Inflammatory chronic prostatitis / chronic pelvic pain syndrome (CP/CPPS), also referred as NIH category IIIA
prostatitis, is characterised by an abacterial inflammation of
the prostate, associated with pelvic pain. It is the most
common type of prostatitis in human.
Inflammatory CP/CPPS is induced by intraprostatic injection of carrageenan; a seaweed polysaccharide which is
widely used to induce inflammatory pain in animal models.
SPECIE
Rat
ns
ns
1.5
1.0
ns= p>0.05, ** p<0.01,
Kruskal-Wallis test. (n=6-16)
Values represent mean ± s.e.m.
0.5
0.0
Naive
MODEL DESCRIPTION
• The pelvic sensitivity to mechanical stimuli is assessed
using von Frey filaments of different forces that are applied to the scrotum area.
• Mechanical stimulations are performed, in conscious animals, at different time points after carrageenan injection
(from 1d to 7d).
• Tested compounds can be administered via various
routes (i.v., i.p., s.c., p.o.).
Nociceptive scores (%)
B
60
Naive
50
Carrageenan
30
***
20
10
0
0.0
0.5
1.0
1.5
ns= p>0.05, *** p<0.001,
Two-way ANOVA with repeated
mesures.(n=6-16)
Values represent mean ± s.e.m.
2.0
C
ns
ns
80
***
***
70
60
50
40
ns= p>0.05, *** p<0.001,
One-way ANOVA. (n=6-16)
Values represent mean ± s.e.m.
30
20
10
0
Naive
Saline
Carrageenan
Intraprostatic injection
Ibuprofen (300 mg/kg, p.o.) and morphine (3 mg/kg, s.c.)
reverse carrageenan-induced pelvic pain.
PARAMETERS EVALUATED
80
• Nociceptive threshold
• Area under the curve (AUC) by plotting nociceptive
scores against von Frey force
SCIENTIFIC PUBLICATIONS
Chen CS et al., Prostate 73: 391-7, 2013
Mosli HA et al., BJUI int. 110: 274-82, 2012
Chen RZ et al., J. Ethnopharmacol. 134: 1018-23, 2011
Radhakrishnan R, Inflammopharmacol. 17: 23-28, 2009
UROsphere is a pre-clinical company specializing in in vitro and in vivo pharmacological
studies for Urogenital and Renal disorders - http://www.urosphere.com
***
***
70
AUC (0.16-2 g)
• Nociceptive scores (%)
•
•
•
•
ns
Saline
40
von Frey forces (g)
AUC (0.16-2 g)
• This model of inflammation-induced pelvic pain is a rapid
and relevant preclinical model to test therapeutic approaches for the treatment of inflammatory CP/CPPS.
Saline Carrageenan
Intraprostatic injection
INTEREST
• This model is characterized by allodynia (decreased
threshold in response to innocuous von Frey forces) and
hyperalgesia (increased nociceptive scores in response
to noxious von Frey forces).
• Compounds that reverse carrageenan effects in this
model include: a non-steroidal anti-inflammatory drugs
(ibuprofen) and an opioid receptor agonist (morphine).
**
**
60
50
40
30
20
10
0
Vehicle
Ibuprofen
Vehicle
Morphine
Carrageenan Intraprostatic injection
*** p<0.001, Unpaired t test. (n=4-7)
Values represent mean ± s.e.m.
© UROsphere 2014. All rights reserved. Non contractual document
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