SOP DSC 8000 ‐ PERKIN ELMER How to use the DSC 8000 ? ‐ Switch on computer and Log in under your user name. ‐ Login on this link: http://lppc35.epfl.ch/CAE1200/cae.html to switch on the instrument. ‐ Switch on the intracooler. Before starting the analysis the intracooler has to be switched on at least for 1h30! ‐ Open Software Pyris Manager ‐ Double click on DSC 8000 at the top left of the screen. ‐ Open the method Editor. (the window is usually already opened). ‐ Create a method: Sample info: o o o o Initial State: o Sample ID Operator ID Save Data As Browse (save the sample under data and your file ending with _###) Enter Sample Weight. Set initial Values: If you use the autosampler set 30°C. The autosampler cannot be used at a lower temperature (you have to load it manually). Program: enter values and program o Minimum chamber temperature during loading: 30°C. o Maximum temperature with aluminum pans: 600°C. o Add/Modify step, put the initial/final temperature. Heating rate between 5 and 50 will give accurate results. View program: summary of the method ‐ Open File Save Method As… (Folder: C:/Program Files/PerkinElmer/Pyris/methods/yourusername.). ‐ Select the calibration: According to the rate chosen you have to select the adequate calibration. When the Method editor is open the Calibration menu is available. Instrument Calibration Press OK Choose the calibration according to: o Rate o o Material pan Cooling system If the calibration needed doesn’t exist, please contact Cindy Känel. Never modify a calibration yourself. ‐Control Panel: Autosampler Control: This option is usable if you have only one sample to analyze. If you have more samples just go directly on the explanation of the Pyris Player. Autosampler Control: o Set temperature: 30°C o Load Sample from location o Load reference from location. A12 o Load sample lid. L1 o Load reference lid. L3 You can open/close the cover via the autosampler control or you can use the DSC COVER open/close on the instrument. Don’t leave the cover open more than 1 minute to avoid ice on the sample holder. When everything is in place, you can press the button Start/Stop. (When the vertical red line is on the left: off; on the right: on) ‐ Open the Player Editor: 1. Prepare Sample: 1.1: Load Reference A12. Load Lid From L3. 2. Sample Group: 2.1: Sample List. Add Sample 2.1.1: Sample ID 2.1.2: Operator ID 2.1.3: File Name. Browse… 2.1.4: Weight: 18.104.22.168: Use Lid1 22.214.171.124: Return to Tray or Return to Waste 2.1.5: Location Clic on the 1st sample and Copy than Past on the 2nd sample Edit the weight and the location for your sample. 2.2 Data Analysis List: 2.2.1: Clic on it, Add a Step, Choose Display Curve. Use Current Run 2.2.2: Add a step, Calculation options, select Peak Area. ‐ Save the player ‐ Press Start. Data Analysis: Press on the data analysis icon and start the analysis Switch off the instrument: At the end of your measurement, switch off the Intracooler. Raise the sample temperature to 200°C and let the software on. Wait until room temperature to turn off the software. At least 3h.
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