MD ALMAMUN M272 Medical Science Building. One Hospital Drive. Department of Pathology and Anatomical Science School of Medicine University of Missouri-Columbia Columbia, MO 65212 Phone: (573) 884-2093, E-mail: [email protected] Education PhD Student in Pathobiology Area Program; Department of Pathology and Anatomical Science University of Missouri, Columbia. MO. Advisor: Dr. Kristen Taylor Master of Science (M.S) in Animal Science (Reproductive Physiology/Developmental Epigenetics); University of Missouri, Columbia, MO. Advisor: Dr. Rocio M. Rivera Thesis: Size-Dependent Acquisition of Global DNA Methylation in Oocytes is Altered by Hormonal Stimulation. Doctor of Veterinary Medicine (D.V.M.), Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. 2003. Skills Proficient Molecular biology techniques – DNA and RNA isolation, Bisulfite conversion of DNA, PCR, RT-PCR, Acrylamide and agarose gel electrophoresis. DNA, RNA library preparation for NextGene sequencing. Methylated CpG Island Recovery Assay (MIRA) Chromatin Immunoprecipitation (ChIP) assay. Immunohistochemistry and immunofluorescence. Molecular cloning. Cell culture and nucleated cell sorting from umbilical cord blood. Collection of oocytes, one-cell, two-cell, morulla, and blastocyst stage embryos from mice. Transrectal B-mode, color- and power-Doppler mode ultrasonography; monitoring follicular and luteal development, pregnancy, blood flow of ovarian follicles and corpus luteum in bovine and equine. Transvaginal ultrasonography; ovarian follicular aspiration for collection of follicular fluid, granulosa cells and oocytes, follicle ablation, and intrafollicular injection. Rectal palpation (bovine and equine); reproductive status, ovarian activity, uterine and cervical conditions and artificial insemination. Vein and uterine catheterization in bovine and equine. Familiar Flow Cytometry. In vitro mouse embryo culture. Small reproductive surgeries, ovariectomy by flank, and cesarean. Cytological and histological slide evaluation. Semen handling (bovine): collection, evaluation, artificial insemination. Frozen semen techniques (bull). Various laboratory tests in undergraduate study. As a Product Executive Prepared product wise sales budget, marketing and promotional activities and implementing strategies, promotional and training materials for Dairy and Poultry medicine and nutritional products. Provided requires inputs in the process of new product development. Building relationship with the Veterinarian, dairy and poultry farmers. Provided training on medicine and nutritional products to the sales personnel. Application software: MS Office, MS Excel, Power point, SAS, Minitab, Sigma Plot, Adobe Illustrator, etc Work Experience Graduate Research Assistant (Fall 2011-Present). Department of Pathology and Anatomical Science University of Missouri-Columbia. Teaching Assistant (Spring 2011). Course: Survey of Epigenetics. University of Missouri-Columbia Graduate Research Assistant (April 2009- May 2011) Division of Animal Science, University of Missouri-Columbia. Research Assistant (April 2007- March 2009) Eutheria Foundation, 4343 Garfoot Road, Cross Plains, Wisconsin 53528, USA. Research Topics: Relationship between preovulatory follicle wall blood flow and oocyte competence at fertilization and embryo development. Product Executive, Animal Health. (February 2006- February 2007). ACI Limited, ACI Centre, 245 Tejgaon Industrial Area, Dhaka-1208. Bangladesh Customer Services Officer (August 2004-January 2006) ACI Animal Health, 611/A Mehedibag, Shahid Mirza Lane, Chittagonj. Bangladesh . Awards and Scholarships Graduate Research Assistantship (Summer 2009 - Present) at MU. Finalist of Trainee Research Award; Society for the Study of Reproduction. Poster Competition. Milwaukee, WI. July 2010. Graduate student Association Travel Grant (Summer 2010). Division of Animal Science, University of Missouri, Columbia. Travel Award (Summer 2010) from Graduate Professional Council (GPC) at MU District Merit Award 1999, Mymensingh, Bangladesh. Student Stipend from Bangladesh Agricultural University (Sept. 1998- June 2003). Publications Abstracts Almamun, Md. and Rivera RM. 2010. Size-dependent acquisition of global DNA methylation in oocytes is altered by hormonal stimulation. Biol Reprod; 83 (1 Supplement) 312. Invited Chapter Huffman, S.R., Almamun, Md and Rivera, R.M. (2011 - Accepted). Isolation of RNA and DNA from single preimplantation embryos and small number of mammalian oocytes for imprinting studies. Protocol book on Genomic Imprinting. Methods in Molecular Biology (Humana Press, USA). Refereed Journals Almamun Md and Rivera R.M. Size-dependent acquisition of global DNA methylation in mouse oocytes is altered by hormonal stimulation. (In progress). Ginther OJ, Almamun M, Shahiduzzaman AK, Beg MA. 2010. Disruption of the periovulatory LH surge by a transient increase in circulating 17beta-estradiol at the time of ovulation in mares. Anim Reprod Sci. 117(1-2):178-82. Siddiqui MA, Gastal EL, Gastal MO, Almamun M, Beg MA, Ginther OJ. 2009. Relationship of vascular perfusion of the wall of the preovulatory follicle to in vitro fertilisation and embryo development in heifers. Reproduction. 4:689-697. Siddiqui MA, Almamun M, Ginther OJ. 2009. Blood flow in the wall of the preovulatory follicle and its relationship to pregnancy establishment in heifers. Anim Reprod Sci. 113:287-92. Ginther OJ, Almamun M. 2009. Increased frequency of double ovulations after induction of luteolysis with exogenous prostaglandin F2α. Journal of Equine Veterinary Science. 29(7):581-583. Presentations Posters: Md Almamun and Rocio Rivera. Size-dependent acquisition of global DNA methylation in oocytes is altered by hormonal stimulation. Graduate Student Forum Seminar, 2010, Division of Animal Sciences, University of Missouri-Columbia. Society for the Study of Reproduction (SSR) Annual Conference. Milwaukee, WI. July 30 to August 03, 2010. Oral : Size-dependent acquisition of global DNA methylation in oocytes is altered by hormonal stimulation. Joint lab meeting. Division of Animal Sciences, University of Missouri, Columbia. September 2010. Identification of epigenetic alterations in oocytes induced by ovarian hyperstimulation. Joint Lab meeting. Division of Animal Sciences, University of Missouri, Columbia. October 2009. Invited Speaker: Graduate level Epigenetics Course (Animal Science 8415). University of Missouri (May 2011). Title: Size-dependent acquisition of global DNA methylation in oocytes is altered by hormonal stimulation. Species Experience Human tissue samples Cell culture Cattle Horse Mouse Chicken Professional Memberships Society for the Study of Reproduction (SSR) – 2010. Bangladesh Veterinary Association (BVA) - 2005-2007. Languages Bangla English Extracurricular Activities Student member of Graduate student Association, Division of Animal Sciences, University of Missouri, Columbia. 2009 – 2011. President of Bangladesh Student Association, University of Missouri, Columbia. 2010 – Present. Member of Bangladesh Association of Agricultural Scientists in America. 2009-2010. Member of The Vet Executive, Bangladesh. 2006.
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