David W Reid, PhD Education Research experience

David W Reid, PhD
Research Fellow
Duke-NUS Medical School
National University of Singapore
8 College Rd
Singapore 169857
Email: [email protected]
Tel: +1 541.206.5222
Citizenship: USA
Duke University, Durham, NC
PhD, Biochemistry
Thesis: Segregation of protein synthesis between the cytoplasm and endoplasmic reticulum of
eukaryotic cells
Advisor: Christopher V Nicchitta
University of Oregon, Eugene, OR
BS with Honors in Biology, minor in Biochemistry
Thesis: Identification of Drosophila genes that modify the activity of Helicobacter pylori CagA
Advisor: Karen J Guillemin
Research experience
Research Fellow, Duke-NUS Medical School, National University of Singapore
Advisor: Shirish Shenolikar
Investigated the changes in protein synthesis that are associated with disruptions in
proteostasis, particularly in models relevant to Parkinson’s Disease.
Graduate student, Department of Biochemistry, Duke University
Advisor: Christopher V Nicchitta
Integrated biochemical and computational tools to probe the role of mRNA localization in
translational regulation and cell stress responses
Rotation student, Department of Biochemistry, Duke University
Advisor: Lorena S Beese
Purified and crystallized farnesyltransferases from pathogenic fungi, obtaining highresolution structures
Advisor: Jack D Keene
Developed computational techniques to analyze high-throughput sequencing data for
RNA binding protein footprinting experiments
Research Assistant, Institute of Molecular Biology, University of Oregon
Advisor: Karen J Guillemin
Developed and employed a Drosophila model of Helicobacter pylori pathogenesis,
focusing on the effect of the pathogenicity factor CagA upon cell junction integrity
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David W Reid – Curriculum vitae
Comment on "Principles of ER cotranslational translocation revealed by proximity-specific
ribosome profiling"
The Unfolded Protein Response Triggers Selective mRNA Release from the Endoplasmic
A novel ribosomopathy caused by dysfunction of RPL10 disrupts neurodevelopment and
causes X-linked microcephaly in humans.
De novo translation initiation on membrane-bound ribosomes as a mechanism for
localization of cytosolic protein mRNAs to the endoplasmic reticulum
Multifunctional roles for the protein translocation machinery in RNA anchoring to the
endoplasmic reticulum
Premature translational termination products are rapidly degraded substrates for MHC Class
I presentation
The enduring enigma of nuclear translation
Primary role for endoplasmic reticulum-bound ribosomes in cellular translation identified by
ribosome profiling
Hierarchical regulation of mRNA localization to the endoplasmic reticulum
Integrative regulatory mapping indicates that the RNA-binding protein HuR (ELAVL1) couples
pre-mRNA splicing and mRNA stability
Identification of genetic modifiers of CagA-induced epithelial disruption in Drosophila
Publications: 12 (7 first-author) | Total citations: 341| h-index: 5
Simple and inexpensive ribosome profiling analysis of mRNA translation
Reid DW, Shenolikar S, Nicchitta CV
In revision, Methods
Diversity and selectivity in mRNA Translation on the Endoplasmic Reticulum
Reid DW, Nicchitta CV
Nature Reviews Molecular Cell Biology. 2015 Apr;16(4):221-231
Featured Article, April 2015 issue
Reid DW, Nicchitta CV
Science. 348, 1217
Reid DW, Chen Q, Ling TS, Shenolikar S, Nicchitta CV
Cell. 158(6):1362-74
Featured in Nature Chemical Biology
Brooks SS, Wall AL, Golzio C, Reid DW, Kondyles A, Willer JR, Botti C, Nicchitta CV, Katsanis N, Davis EE
Genetics. 198(2):723-33
Jagannathan S, Reid DW, Cox AH, Nicchitta CV
RNA. 2014(20):1489-98
Jagannathan S, Hsu JC, Reid DW, Chen Q, Thompson WJ, Moseley AM, Nicchitta CV
Journal of Biological Chemistry. 289(37):25907-24
Lacsina JR, Marks OA, Liu X, Reid DW, Jagannathan S, Nicchitta CV
PLoS ONE. 7(12):e51968
Reid DW, Nicchitta CV
Journal of Cell Biology. 197(1):7-9
Reid DW, Nicchitta CV
Journal of Biological Chemistry. 287(8):5518-27.
Featured in F1000
Chen Q*, Jagannathan S*, Reid DW*, Zheng T, Nicchitta CV
Molecular Biology of the Cell. 22(14):2646-58
Mukherjee N, Nusbaum J, Corcoran D, Reid DW, Georgiev S, Hafner M, Ascano M, Tuschl T, Ohler U, Keene JD
Molecular Cell. 43(3):327-39
Reid DW*, Muyskens JB*, Neal JT*, Gaddini GW, Cho LY, Wandler AM, Botham CM, Guillemin K.
Frontiers in Microbiology. 2012(2):24
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David W Reid – Curriculum vitae
Gordon Conference: Translational Machinery in Health and Disease, Ventura, CA
The unfolded protein response triggers selective mRNA release from the ER
Duke-NUS Annual Retreat, Singapore
Selected talk: Release of mRNAs from the ER as a stress response mechanism
Awarded best selected presentation
ASBMB: The Endoplasmic Reticulum in Health and Disease, Warrenton, VA
Selected talk: A primary role for ER-bound ribosomes in cellular protein synthesis
Triangle Center for RNA Biology, Chapel Hill, NC
A primary role for ER-bound ribosomes in cellular protein synthesis
ASCB Annual Meeting, Denver, CO
A primary role for the endoplasmic reticulum in protein synthesis
Barry M Goldwater Scholarship, University of Oregon nominee
William L Martin Scholarship
Dean’s Scholarship, University of Oregon
Volunteering and community involvement
Board of Trustees Member, Duke University, Facilities & Environment
Oversaw University strategy, planning, and operations for construction and
environmental initiatives
Graduate student representative, Duke Committee on Facilities & Environment
Advised the Board of Trustees on construction, transportation, and finances.
Particularly active in alternative transportation
Demonstrator, Science Night, Scroggs Elementary, Carrboro, NC
Demonstrated properties and principles of acids and bases to third through fifth
graders at an annual science night
Advisor, Little Flower High School, Philadelphia, PA
Advised high school students interested in science about education and careers
Member, Durham Bike Co-op, Durham, NC
Helped community members build and repair their own bikes
Executive Board Member, National Intercollegiate Running Club Association
Led club recruitment, marketing, and competition efforts for the national
running club organization, helping the group to double in size
Coordinator, University of Oregon Running Club
Directed fund raising, budgeting, team selection, and travel, culminating in a
national championship
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David W Reid – Curriculum vitae
Technical expertise
Deep sequencing techniques: Ribosome profiling, mRNA-seq, small RNA-seq
Computational analysis: Python, R, LINUX, Bowtie, standard deep sequencing toolsets
Biochemical techniques: Ultracentrifugation, metabolic labelling, cell culture, PCR
Model organisms: Mammalian cell culture (including primary and neurons), fruit fly, zebrafish
Teaching and mentoring
High school student mentor
Hosted and advised a high school student over two summers, introducing her to
biochemical and computational techniques and career choices
Teaching Assistant, Department of Biochemistry, Duke University
Course: Biochemistry II
Advisor: Michael D Been
Designed and presented weekly lectures to reinforce material
Teaching Assistant, Department of Biology, University of Oregon
Course: Microbiology Laboratory
Advisor: Alan J Kelly
Helped students perform and interpret experiments, developed new curricula
Shirish Shenolikar – Professor, Duke-NUS Graduate Medical School
Post-doctoral advisor
Phone: +65.6516.2588
Email: [email protected]
Christopher Nicchitta – Professor of Cell Biology and Biochemistry, Duke University
Graduate advisor
Phone: 919.684.8948
Email: [email protected]
Karen Guillemin – Professor of Biology, University of Oregon
Undergraduate advisor
Phone: 541.346.5999
Email: [email protected]
Jack Keene – Professor of Molecular Genetics and Microbiology, Duke University
Collaborator, thesis committee member
Phone: 919.684.5138
Email: [email protected]
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