Newsletter 1. 2015 Kem-en-tec Nordic Competent BioScience since 1987 Improve your NGS - 100 % conversion rate Mirus Transfection reagents - Non Toxic! ELISA - We are the experts - Reagent and kits - Mini workstation and washer - Multilabel readers High Throughput DNA Electrophoresis - without gels! Liquid handling - Easy and Fast Centrifuge/vortexer - For DNA prep work - Patented unique solution Nordic Contact info: Finland +358 9594822 Sweden/ Norway +46 18 149000 Denmark +45 39271777 [email protected] All prices are valid until 30. June 2015. Local currency exclusive VAT and Ex Works. ELISA – Mini workstation ELISA plate washer Set it up with your protocol and leave it to dispense – incubate – wash and finally read your plate. Programmable 8-channel washer with weight logger module for automatic level control Try it - We offer FREE demo in your own lab. Campaign price 30.000,- DKK · 38.500,- SEK 36.000,- NOK · 4050,- € Campaign price For both round and square well plates - 8-well Strips and whole 96 well format 155.000,- DKK · 198.750,- SEK 186.750,- NOK · 20.950,- € Hoefer quality at 25% discount The golden standard in solutions for Electrophoresis - Save 25 % on ALL Hoefer orders. Package example – Request specific quote 2 Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777 Take advantage of our spring offer! ELISA Substrate – ECO-TEK™ - Safe and easy Kem-En-Tec ECO-TEK™ TMB substrates High quality ELISAkits for your research eg Hormones & Steroids Research ELISA Test Kits: Hormones & Steroids Reduce your exposure to carcinogenic reagents Ready-to-use ELISA substrates - So Easy Androstenedione Corticosterone Ordering: (Cat # 4380A) Cortisol 1 litre of TMB ONE ready to use HRP Substrate Estradiol DKK Testosterone SEK NOK Estriol € Progesterone 1.760,-2.260,-2.125,- 240,- Try in your ELISA – Request FREE SAMPLE ECO-TEK™ products are environmentally-friendly components Spring offer Get 10 % on ALL kits Eicosanoids Leukotriene B4 Leukotriene C4 Leukotriene C4/D4/E4 Prostaglandin E2 Prostaglandin E2 (Monoclonal) Prostaglandin F2α 6-keto-Prostaglandin F1α 11β-Prostaglandin F2α 13,14-dihydro-15-ketoProstaglandin F2α Thromboxane B2 11-dehydro-Thromboxane B2 Oxidative Stress Lipoxins Histamine Cytokines Specialty Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com 3 Copenhagen LabMed Booth no 6 · t e d e t s k r komotivvæ Working What’s on display? with Plates? Lo Easy & Fast liquid handling and reading Capillary electrophoresis Fragment Analyzer – the perfect tool for your DNA and RNA analysis Multimodel Microplate Reader HIDEX Sense Molecular biology • Big Touchscreen • Pre-programmed applications • Small footprint • Extremely easy to use • Beta-counting/LSC • 1-1536 wells - ELISA and many other applications See the newest clever gadgets for visualization of DNA See in the video how quick and easy you get your results. The world’s most efﬁcient polymerases Plate handling Experience the Kapa Biosystems solutions ﬁrst hand CyBi®-FeliX CyBi®-SELMA • With 96 and 384 channel pipetting heads • Precise 96 or 384 channel pipetting with the possibility to work in single wells, rows and columns • Interchangeable pipetting heads • Electrophoresis Very small footprint • Semi-automatic 96- or 384-fold pipettor with minimal required space • Easy changing of tips • Remembers height of plates! and Fractionation MW protein fractionator GelCyBio•AG Free8100 – An ideal pre MS analysis 20 years of tool German • RunBlue SDS-PAGE – Precast gels with Qualitysuperior resolution and so strong that they show no tears! • InstantBlue – The 15 min Coomassie – Quick and Easy gel staining See the video ELISA • Crocodile ELISA mini workstation New Tristar² S Multilabel reader with monochromator- great introduction discounts Request – Run your ELISA for you • Plate washers 8 and 96 channel references – Also good for Plate coating ELISA Readers & automation – and•demo! LED based solutions • ELISA Reagents – Ready to use and safe Lab instruments See and try the hot new laboratory instruments from BioSan See the video 4 Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777 October 7-9, 2014 Visit our booth Save money on your transfection reagents Learn tips and tricks from our product specialists Attend a demonstration and have a chance to win prices Ingenio® Electroporation Products See last page for information regarding address and tickets High efficiency electroporation using any electroporator • High efficiency electroporation of hard to transfect cell lines and primary cells • Compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX® • Ideal for plasmid or siRNA electroporation • Save money and reduce research costs while maximizing results Ingenio® Solution Provides Comparable Efficiency on Amaxa® Nucleofector® Device Liquid handling Buy One, Get One For Free* CyBi®-SELMA Semi-automatic 96- or 384-fold pipettor * offer is valid to the end of June 2015, please write NB0415 on order NEW! Liquid handling CyBi®-FeliX Compact pipetting platform for fully automated single- to multichannel liquid handling TransIT-X2® Dynamic Delivery System A novel, polymeric system for efficient delivery of plasmid DNA and/or siRNA/miRNA TransIT-X2® System Enables Superior Gene Expression v.s. Lipofectamine® 2000 in a Variety of Cell Types • Efficient–Exceptional Broad Spectrum DiagnosticsTransfection and research • Versatile–Cutting Edge Delivery of Plasmid kits DNA and siRNA • ELISA • • Novel PCR Technology-Non-Liposomal, Polymeric elivery • D NGS kits Research reagents for • Glycobiology, • Cell biology • Flow cytometry Multilabelreader Most of the news will also be shown at Lab14 in Norway, see last page for more information Sense: Microplate reader with touchscreen – it has ® never been easier TransIT-X2 enables superior or similar Gene Expression in 26 out of 30 cell types compared with Lipofectamine® 2000 using a plasmid DNA encoding luciferase. Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Three different reagent-to-DNA ratios were used. Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com 5 KAPA HYPER PLUS NGS library preparation. Evolved. Bronwen Miller, M Kapa hyperplus – a single-tube ngs library prep workflow integrating enzymatic fragmentation INTRODUCTION Benefits include: Continuous improvements to library • DNA fragmentation and library prep inpreparation 2.5 hours for next-generation sequencing (NGS) to achieve • flexible DNA sample input from 1 ngare - 1 necessary ug • reduced bias andquality. more uniform the highest data One of sequence the crucialcoverage steps within library • PCR-Free workflows preparation is the initial DNA fragmentation, which can be H SE ERPLUS – A SINGLE-TUBE NGS LIBRARY P INTEGRATING ENZYMATIC FRAGMEN IN HIGH YIELDS AND LOW SE accomplished through either mechanical or enzymatic processes. Mechanical methods for DNA fragmentation The KAPA HyperPlus kit provides a streamlined are difﬁcult to scale or automate, and require large investments workflowinstrumentation. that includes fragmentation and libraryfor in expensive Current enzymatic solutions preparation in a typically single tube. Building on industry-leading DNA fragmentation exhibit sequence bias, provide poor control over fragment length distribution, and are solution highly library construction efficiencies, this integrated sensitive to input amount.fragmentation, combines enzymatic similar in quality to Single Tube Fragmentation 10-45 min 30 min End Repair and A-tailing Adapter Ligation Benefits include: 15 min • DNA fragmentation and library prep in 2.5 hours • flexible DNA sample input from 1 ng 1 ug Bead Cleanup 30-min • reduced bias and more uniform sequence coverage The con Figu cons The inpu DNA KAP for b Kets, Beverly Rooyen, Heather Whitehorn, Martin• Ranik, Piet Jones, Adriana Geldart, R with the and convenience To mechanical addressvan theseshearing, challenges, we havespeed developed the KAPA PCR-Free workflows Library Amplification HyperPlus Library Preparation Kit by integrating an enzymatic of tagmentation-based workflows. DNA fragmentation technology with fast and efﬁcient library construction to provide a streamlined, easy-to-automate, single-tube solution for preparing NGS libraries from 1 ng – 1 µg of dsDNA. (optional) 30 min Bead Cleanup 30 min For human exome libraries, the HyperPlus workﬂow show Total time: enzymatic-fragmentation methods and leads to signiﬁcant ~2.5 hourswith high-quality dropout is similar for all three workﬂows Nextera with the FFPE sample. ELDS LEAD TO IMPROVED SULTS TUNABLE AND nversion ofFRAGMENTATION input DNA to adapter-ligated library. This higherIS library lex libraries and improved sequencing metrics, including lower duplicate rates. REPRODUCIBLE 90 Reproducible library fragment size distributions are obtained from a variety of DNA sample inputs. 80 70 AT-dropout Covaris + Hyper Prep 10 Conversion Rate Ranges 8 (%) 40 20 10 0 60 2 50 40 0 30 GC-dropout Figure 1: Fragmentation proﬁles obtained with different DNA inputs. 12 100 ng 10 ng Input Amount 1 ng Conversion rate, defined as % input DNA converted to sequenceable, adapterDuplication Rates Figure 4. HyperPlus exomeultimately capture libraries ligated library, is a key library construction metric, which determines generate fewer duplicate reads than libraries library diversity and quality. Covaris + Hyper Prep HyperPlus produced using Covaris. Roc pre imp 25 6 20 4 15 (%) 1 µg Covaris + Hyper Prep Various input amounts of E.coli gDNA, were processed using the HyperPlus HyperPlus Kit10with fragmentation times of 15 or 30 minutes at 37˚C. Nextera samples After library ampliﬁcation and a single 1X SPRI® bead cleanup, 8 were analyzed using an Agilent High Sensitivity DNA Assay™. (%) 10 0 FFPE gDNA 20 2 6 50 30 Existing solutions for NGS library construction are extremely sensitive to 90 commercial enzymatic fragmentation HyperPlus 6 sample80type and input amount, but the KAPA HyperPlus Covaris + Hyper PrepKit provides reproducible results across a range of inputs. Covaris Nano Prep 4 Library70insert sizes can be adjusted by varying the+ Illumina fragmentation time. % Conversion version of -sheared it or the yperPlus 100% Achieve conversion rate Nextera 100 ibrary HyperPlus Duplicates (%) 60 12 A. Libraries were prepared from 200 ng of high molecular weight FFPE DNA (403s, 414s) or 0 FFPE DNA (204s, 214s) and captured Kem-En-Tec Nordic ·degraded Finland +358 9594822 · Sweden/Norway +46 18 149000 ·gDNA Denmark +45 3927 1777 FFPE with the Roche Nimblegen™ double-capture protocol (Data courtesy of Dr. Brian Walker 10 5 0 DNA Analyzer Analyze 48 96-well plates in one day! -The easiest DNA fragment analysis ever. Place your PCR plate directly from the thermocycler into the ZAG for a complete analysis in 20 minutes. Automated data output which can easily be integrated into a LIMS. ZAG Zero Agarose Gel DNA Analyzer For fewer samples or RNA, we offer The Fragment Analyzer. Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com 7 k ec Ch g ng S! op rin buyi om OB bsh rde start ic.c e o d rd W sy ser an c-no ea u te r ate nfo cre -e – m ke w. w w t ou it y! as –e New revolutionary Centrifuge Meet us! Meet the Worlds first combined centrifuge/vortexer for PCR plates Exhibitions/ Conferences in Nordic 2015 The patented Spin–Mix–Spin technology is intended to spin-down micro volumes of reagents on the well’s bottom (first centrifugation spin), following mixing (mix) and spin-down the reagents again from the walls and cap of the well (second spin). Ideal for sample prep. Denmark April 25-28 ECCMID the 25th European Congress of Clinical Microbiology and Infectious Diseases in Bella Centre May 20 Rigshospitalets Symposia – At Rigshospitalet Patented technology May 27-29 DSKB – Danish society of Clinical Biochemistry Sep 23-24 LabDays – Aarhus, Scandinavian Congress Center at Radisson Blu Sweden May 5-6 SLAS (Society of Laboratory Auto mation & Screening) & Nordic Biology Meeting, Karolinska Solna May 6-7 Clinical Immunology Spring Meeting, Lund Campaign price 9.175,- DKK 11.750,- SEK 11.050,- NOK 1.234,- € (incl rotor for 2 PCR plates) PS: The front page shows the endangered blue-footed Sule from the Galapagos Islands, sponsored of our supplier KAPA Biosystems. 8 Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com June 16-17 ELRIG (European Laboratory & Research Innovation Group), Astra Zeneca Mölndal November NOV2K (Karolinska) Finland March 19 Biocenter Day Oulu March 26-27 Virology Days, Kuopio! Visit us and win nice prizes and knowledge!
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