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A.O. Majolagbe et al / Journal of Environment (2013), Vol. 02, Issue 01, pp. 19-24
ISSN 2049-8373
Research Paper
Concentration and Bioavailability of Iron in Some
Selected Blood-Building Medicinal Plants in Southwest
Nigeria
A.O. Majolagbe1*, V. Kuteyi2, C.T. Onwordi1 and K.A. Yusuf1
1
2
Department of Chemistry, Lagos State University, P.M.B. 1087, Apapa, Lagos, Nigeria
Department of Science Laboratory, Yaba College of Technology, P.M.B. 0001, Yaba, Lagos, Nigeria
*E-Mail: [email protected] ; [email protected]
Abstract
The use of medicinal plants for blood-building in Sub-Saharan Africa is a common practice. However, this practice is being
challenged by the inadequate data on the bioavailability of both nutritional and toxic elements in the plants being used. In
this study, the total iron (Fe) concentration in five (5) medicinal plants parts used by local communities in South Western
Nigeria to treat anaemia: Leaves of Sorghum bicolor (Poroporo Baba), Bark of Magnifera indica (Mango), Telfaria
occidentalis (Ugwu), Basella alba (Amunututu) and leaves of Hibiscus sabdariffa (Zobo) were determined employing
Flame Atomic Absorption Spectrophotometry (FAAS). The level of iron observed in the plants studied, classifies them as
high iron source going by WHO range of 70-200 µg/g. S. bicolor showed the highest concentration of 188.7 µg/g Fe while
leaves of H. sabdariffa showed the least concentration of 69.7 µg/g Fe. Speciation patterns of Fe were also investigated
using four-step Bureau Community of References’ (BCR) selective sequential extraction technique to evaluate the
bioavailability of iron in the plants. The result showed that S. bicolor has the highest bioavailable Fe with a concentration of
121.8 µg/g. The bioavailable Fe in the plants is in the order S. bicolor > Barks of M. indica >B. alba > T. occidentalis >
leaves of H. sabdariffa.
Keywords: Speciation, Iron, Bioavailability, Blood-Building, Medicinal Plants, Spectrophotometer
1. Introduction
The philosophy of link between human life and elements
dates back to the inception of practice of medicine (Cebi et
al, 2011). Various metabolic activities in normal physicalogical processes are known to relate with metal ions, with
specific importance by every element in life system. Iron,
for instance, is a mineral element that plays a crucial role
in the human system. It produces red blood cells (haemoglobin) which carry oxygen throughout the body and stores
in myoglobin, an oxygen-carrying protein in the muscles
that fuel cells growth. Iron is an essential element for man
and animals. It is an essential component of haemoglobin
and facilitates the oxidation of carbohydrates, protein and
fat, thereby controlling body weight, which is very important factor in diabetes (Khan et al, 2008). The use of
medicinal plant to treat various body diseases is gaining
more patronage worldwide which may be attributed to
relative low cost of herbs, low side effects of natural herbs
as compared to synthetic drug (Kirmani et al, 2011) and to
a large extent the increase in awareness and confidence
that medicinal plants contain valuable compound especiallly in Africa. Omolo et al (1997) carried out an investigateon on the total and extractable iron species in eight Eastern
African medicinal plants used traditionally against anemia.
It is reported that, whatever that is taken as food could
cause metabolic disturbance, subject to the allowed upper
and lower limits of trace metals (Prasad, 1976 and Khan et
al, 2008). Both the deficiency and excess of essential
micronutrients and trace of toxic metals may cause serious
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A.O. Majolagbe et al / Journal of Environment (2013), Vol. 02, Issue 01, pp. 19-24
effects on human health (Khan et al, 2008). Excessive
dietary iron is toxic. The excess ferrous iron reacts with
peroxides in the human body, producing free radicals. The
side effects of taking high doses of iron include constipation, nausea, vomiting and stomach pain. Very high doses
of iron can be fatal, particularly in children, a disease
results called hemochromatosis. The excess iron accumulates in the liver, resulting in siderosis and organ damage.
However, iron deficiency anaemia is the most common
form of anaemic conditions in growing children due to
increase in iron demand. The deficiency is also found in
young girls and women due to excessive monthly menstrual bleeding. Other causes are poor digestion, long-term
illness; excessive use of coffee, black tea and the sugar has
been rated as the greatest causes of iron deficiency
anaemia.
Anaemia is characterized by fatigue, shortness of breath,
pale skin, concentration problems, dizziness, a weakened
immune system and energy loss. Other conditi-ons include
hypo-copraemia, hypo-protienemia and dwarf-ism
(Klaassen et al, 1986). Iron deficiency varies greatly with
geographical and socio-economic factors. Infections,
excessive sweating and staple food stuffs like vegetables
or cereals with high bran and phytate content are some of
the factors that account for high anaemic incidence in
developing countries as compared to developed nations
with temperate climatic conditions (Bjorn-Rasmussen,
1974). Regular body activities such as urination, defecateon, sweating and menstruation in women lead to continuous loss of blood. The consequential daily iron loss of body
in adult male is 1.0 mg, while that of female is 0.8 mg
(Mertz, 1987). Conditions like pregnancy and administration of antibiotics also increase the requirement for Iron. In
order to balance this loss, a daily absorption of 2.0 mg of
iron is considered necessary (Anderson et al, 1981). This is
achieved through balanced nutrition; iron fortification or
supplementations supplement.
The history of herbal medicine for amelioration of human
sufferings pre date modern medicine and its continuous
existence till today confirms its validity. Herbal treatments
of ailment are enjoying strong patronage because of their
accessibility and safety, with very few cases of side
reactions or allergy (Rates, 2001). An herb or medicinal
plant is a plant, which in one or more of its organ contains
substances that can be used for therapeutic purposes or
which are precursors for the synthesis of useful drugs.
Worldwide, there is a growing trend for research, development, documentation utilization and promotion of traditional medicine (Abou-Arab & Abou Donia, 2001). The
World Health Organization (WHO) acknowledges the
growing need and potentials of traditional medicine and
continues to encourage and supp-ort research in traditional
medicine with a view to further develop its potentials and
use, especially in developing countries. According to the
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World Health Organization report, almost 80% of people
in marginal communities use only medicinal plants for the
treatment of various diseases (Pirzada et al, 2009). It
further implored countries to fully utilize the positive aspects of traditional medicine to increase access of its citizens
to quality health care services. Traditional medicines have
also become a huge job and wealth creation avenue
worldwide.
Traditional medicines and the use of herbal medicinal
plants have been and will continue to be a strong part of
Nigerian history as well as her culture and beliefs. It is a
fact that over 85% of the population patronizes traditional
practitioners for their health care and other needs
(NNMDA, 2007). In spite of this high patronage, the
products and practices are still highly misunderstood and
mystified. This is largely due to the inadequate and
sometimes lack of information on the composition and the
active ingredients in the plant and toxicity of such
ingredient. Since the toxicity of heavy metals is related to
their existing species, speciation of these cations is increasingly attracting more attentions (Nassef et al, 2006). The
toxicity of heavy metals depends upon the chemical form
of elements (Kirmani et al, 2011). Heavy metals are
dangerous in the form of their cations and are highly toxic
when bonded to the short chains of carbon atoms (Hussain
et al, 2006).
Environmental conditions like type of soil, rainfall,
vicinity of industry and extensive agricultural activity influence the level of bioavailable elements in plants (Konieczyński & Wesołowski, 2007). Unlike in soils, where chemical speciation studies of metals have been extensively
investigated, there still remains little information on metal
speciation in medicinal plants particularly in West African
states. Quite a number of investigators have employed
sequential extraction methods to fractionate heavy metals
in soil and sediments. Speciation studies are carried out by
single or sequential extraction with reagents having different chemical properties. In order to evolve the best
Sequential Extraction Procedure (SEP) for the speciation
metals in medicinal plants, various sequential extraction
procedures were compared (Oyeyiola et al, 2009). The
SEP developed included a modified Tessier’s procedure
carried out in 5-steps, the 3-step original BCR and the four
step modified BCR techniques. The results showed that the
modified BCR and Tessier extract more in the reducible
phase and consequently a decrease in the Oxidizable phase
than the original BCR, hence modified BCR technique was
adopted in this study. Sorghum bicolor (poroporo baba),
Basella alba (Amunututu), Bark of Magnifera indica
(Mango), Telfaria occidentalis (Ugwu) and leaves of
Hibiscus sabdariffa (Zobo) were medicinal plants used in
the study. These medicinal plants are used as blood-builder
with a geographical spread around the three (3) major ethnic groups in Nigeria.
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A.O. Majolagbe et al / Journal of Environment (2013), Vol. 02, Issue 01, pp. 19-24
Though, a number of studies had been carried out on
metals concentration in Nigerian vegetables and medicinal
plants, not much has been reported about speciation pattern
of these cations. This paper is, therefore, aimed at establishing the total concentration of iron in some medicinal
plants used in treatment and management of anaemia in
Nigeria, as well as investigating their speciation pattern.
2. Materials and Methods
2.1. Sampling
Plant samples of Sorghum bicolor (Poroporo baba), Basella alba (Amunututu), Bark of Magnifera indica (Mango),
Leaves of Hibiscus sabdariffa (Zobo) and Telfaria occidentalis (Ugwu) were obtained from the open markets at
Mushin, Igando, Oyingbo, Iju Ishaga and Iyana Iba, all in
Lagos State, South-Western Nigeria. The samples were
identified at Lagos University Herbarium: Sorghum bicolor (LUH 1467A), Basella alba (LUH 1471A), Bark of
Magnifera indica (LUH 1469A), Leaves of Hibiscus
sabdariffa (LUH 1468A) and Telfaria occidentalis (LUH
1470A).
ISSN 2049-8373
having four steps, was employed to speciate the metal load
in the samples into various factions. The steps are as
follows:
Step 1: Exchangeable
40 ml of 0.1 M acetic acid was added to 1.0 g of plant
sample in a 250 ml flat bottom flask. The flask was shaken
for 16 hours at room temperature on a flask shaker at 400
rpm. The solution was filtered and the filtrate was analysed for Iron. The residue was washed with 20 ml of deionised water by shaking for 15 minutes and then filtered. The
supernatant liquid was discarded without any loss of
residue.
Step 2: Reducible
The residue from step 1 was washed and stirred in a 250
ml flask by adding 40 ml of 0.1 M hydroxylamine
hydrochloride solution adjusted to pH 2.0 with nitric acid.
The mixture was shaken for 16 hours at room temperature.
The extract was filtered and analyzed for Iron and the
residue was washed with 20 ml of deionised water.
2.2. Sample Preparation
Step 3: Oxidizable
Composite samples of the various plants were sun-dried
for seven days. The dried samples were crushed with the
aid of pre washed ceramic mortar and pestle and made to
pass through a 2 mm mesh.
To the residue from step 2, 10 ml of 8 M hydrogen
peroxide solution was carefully added in small aliquots to
avoid losses due to violent reaction. The flask was digested
at room temperature for 1 hour with occasional manual
shaking. The flask was placed on a water bath and heated
at 85 oC and evaporated to near dryness. A second aliquot
of 10 ml of hydrogen peroxide was added to the residue
and digestion procedure was repeated. 50 ml of 1.0 M
ammonium acetate solution (adjusted to pH 2 with nitric
acid) was added to the moist residue. The mixture was
shaken and filtered. The filtrate was analyzed for Iron
while the residue was washed with 20 ml of deionised
water.
2.3. Determination of Total Iron
1.0 g of powdered each plant sample was weighed into a
125 ml of Erlenmeyer flask which has been previously
washed with 0.1 M HNO3 and rinsed with distilled water.
4 ml of Perchloric acid, 25 ml concentrated HNO3 and 2
ml concentrated H2SO4 (all chemicals were of analar
grade) were added. The content was mixed and heated,
initially at low heat about 60 oC and then on a hotplate
under an acid fume-hood, the heating continued until dense
white fumes appeared. The mixture was strongly heated
for half a minute afterward. The content was allowed to
cool after heating. 50 ml of distilled water was added after
cooling and boiled further for half a minute on the same
hot plate. The solution was allowed to cool again and
filtered using a Whatman No. 42 filter paper. The solution
was made up to the 100 ml mark with distilled water and
analyzed for iron using 210 VGP Flame Atomic Absorption Spectrophotometer. Each sample was analysed twice to
test it for reproducibility. General laboratory quality assurance measures were observed to prevent sample contamination and instrumental errors.
2.4. Sequential Extraction
The Bureau Community of Reference (BCR) method,
Step 4: Residual
5 ml of distilled water and 12 ml of aqua-regia solution
were added to the remaining residue and evaporated to
near dryness on an electric heater. The procedure was
repeated using 8 ml aqua- regia solution. 20 ml of 0.1 M
nitric acid was then added in small aliquots to the residue
and filtered. The filtrate was analyzed for iron.
3. Results
Table 1 shows detail of the plants analysed including the
names, parts of plant used as well as the medicinal properties of the plants analysed while Table 2 shows the concentration of total iron observed in each of the medicinal
plant investigated as well as in some other plants in
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A.O. Majolagbe et al / Journal of Environment (2013), Vol. 02, Issue 01, pp. 19-24
literature. Table 3 reveals the speciation pattern of iron in
the plants studied and correlation coefficient among the
fractions are given in Table 4. Figure 1 gives the pictorial
representation of percentage fractions in the plants and
Figure 2 gives the dendrogram of the blood building
medicinal plants investigated.
ISSN 2049-8373
Leaves of Hibiscus sabdariffa. Khan et al (2008) suggested
that the high amount of Fe in plants may also be due to the
foliar absorption from the surroundings air.
Konieczyński & Wesołowski (2007) also conducted
research on total and extractable iron in selected herbs
Table 1. Names and Pharmacognostic Features of the Blood Building Plants
Plants Species
Sorghum bicolour
Besella alba
Local Name
Parts Analysed
Medicinal Properties
Poroporo Baba
Amunututu
Stem
Stem
Blood Building
Blood Building
Ugwu
Mango
Ewe Zobo
Leaves
Bark
Leaves
Blood Building
Blood Building, Anti-Malaria
Blood Building
Telfaria occidentalis
Magniffera indica
Hibiscus sabdariffa
Table 2. Total Concentration of Iron in Plant Parts of Present Study
and Other Published Works (µg/g)
Plants Species
Sorghum bicolour
Besella alba
Telfaria occidentalis
Magniffera indica
Hibiscus sabdariffa
Pipper nigrum
Brassica rapa
Trachyspermum ammi
Total Iron
Conc.
189+20
107+7
142+11
167+23
70+4.2
640±7.0
Present study
Present study
Present study
Present study
Present study
Lavilla et al, 1999
8.8±0.01
2486±28
Hashmi et al, 2007
Lavilla et al, 1999
Reference
collected from natural areas in Northern Poland. The total
iron content in medicinal plants in this study compare well
with plants like Brassica rapa (Hashmi et al, 2007) but
lower than Pipper nigrum and Trachyspermum ammi
(Lavilla et al, 1999) as shown in Table 2.
However, the information on the forms (species) of metal
is very important so as to be able to predict the impact of
the metal on human body system. The concentration of
iron in various factions is presented in Table 3. The
bioavailable form of iron in investigated plants revealed
that Sorghum bicolor exhibited high value of 122 µg/g,
compared to Bark of Magnifera indica, Besella alba,
Telfaria occidentalis and Leaves of Hibiscus sabdariffa i.e.
78.8, 68.2, 53.5 and 31.3 µg/g respectively. The bioavaila-
Table 3. Mean Concentration of Iron in Various Fractions (µg/g)
Plant Species
Exchangeable
Reducible
Oxidizable
Residual
Sum of Fractions
Bio-available
Sorghum bicolour
Besella alba
46.6
28.8
41.7
14.5
33.5
24.8
63.7
37.4
186
106
122
68.1
Telfaria occidentalis
Magniffera indica
Hibiscus sabdariffa
7.00
13.2
9.60
42.6
52.8
19.2
3.80
12.5
2.50
85.5
84.0
36.2
139
163
67.5
53.4
78.8
31.3
4. Discussion
The concentrations of iron, given in Table 2, in all the
medicinal plants examined are in the range that classifies
them as high source of iron according to FAO/WHO
(1984) and World Health Organisation (2005). Sorghum
bicolor showed the highest concentration of iron with a
value of 189 µg/g while Hibiscus sabdariffa showed the
least concentration of 70 µg/g. The total iron concentrateons order in the plants is: Sorghum bicolor> Bark of
Magnifera indica> Telfaria occidentalis> Basella alba>
ble fraction of these plants analysed, compared very well
with what was obtained in Poland study (Wesołowski et al,
2007). However, the east African medicinal plants had
very low concentration of bioavailable fractions (Omolo et
al, 1997). Li & Deng (2004) also pointed out that the value
of bioavailable fraction obtained depend on the applied
type of extraction solvent, plant part and the botanical
species of a plant.
The fraction in percentage is presented in Figure 1
Sorghum bicolor: trends in decreasing order of various
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A.O. Majolagbe et al / Journal of Environment (2013), Vol. 02, Issue 01, pp. 19-24
fractions are: Residual> Exchangeable> Reducible> Oxidizable with 65 % of total Fe as bioavailable; Basella alba:
Residual> Exchangeable> Oxidizable> Reducible with
64% as bioavailable; Bark of Magnifera indica: Residual>
Reducible> Exchangeable> Oxidizable with 48 % of total
Fe as bioavailable; Leaves of Hibiscus sabdariffa: Residual > Reducible> Exchangeable> Oxidizable with 47 % of
total Fe as bioavailable and Telfaria occidentalis: Residual
> Reducible> Exchangeable> Oxidizable with 37% of
total Fe as bioavailable. Substantial amounts of Fe are
associated with the residual fractions of all the medicinal
plants examined, an indication that Fe is strongly bonded
in the tissues of the plants. The correlation coeficient
between the fractions as shown in Table 4, reveal that,
there are various sources of what make up total iron concentration. There is a strong positive correlation between
reducible and residual fractions as well as between Oxidizable and exchangeable, an indication of similarity in the
source(s) whiles other fraction reveal negative correlation,
a reflection of uncommon source(s).
ISSN 2049-8373
ble fractions; in addition depletion of substantial amount of
Iron in the plants is associated with the residual fraction.
Under similar conditions, the effectiveness of the various
plants in blood-building based on the bioavailability of
Iron is in the order: Sorghum bicolor> Magnifera indica>
Basella alba> Telfaria occidentalis> Hibiscus sabdariffa.
Table 4. Correlation Coeficiency Between the Fractions Iron Speciated
Speciated Fractions
Exch.
Exchangeable
Reducible
-0.042
Oxidizable
Residual
0.972
-0.232
Red.
0.006
0.933
Oxid.
Res.
Figure 2. Dendrogram of Blood Building Medicinal Plants Investigated
-0.160
It is however recommended that more research work be
conducted in the area of clinical to investigate the rate of
absorption and utilization of Iron in the plants.
Exch.: Exchangeable
Red.: Reducible
Oxid.: Oxidizable
Res.: Residual
5. Conclusion
Figure 1. Percentage of Fractions in Each Plant Analysed
The dendrogram (Figure 2) shows two clusters based on
the strength of similarity; group A (Telfaria occidentalis,
Magnifera indica and Sorghum bicolor and group B
(Basella alba and Hibiscus sabdariffa). Medicinal plants in
group A have higher concentration of residual and reduce-
Based on the findings of this research work, it was
concluded that Sorghum bicolor, Bark of Magnifera indica, Basella alba, Telfaria occidentalis and Leaves of
Hibiscus sabdariffa have high Iron contents and are suitable for use as blood-building herbs. The bulk of the iron is
available to humans and animals. The speciation pattern
revealed that a substantial amount of Iron in the plants is
associated with the residual fraction. The effectiveness of
the various plants investigated, in blood-building is in the
following order: Sorghum bicolor> Bark of Magnifera
indica> Basella alba> Telfaria occidentalis> Leaves of
Hibiscus sabdariffa. This is based on the bioavailability of
iron. It is however recommended that more research work
be conducted in the area of clinical to investigate the rate
of absorption and utilization of iron in the plants.
Acknowledgement
The authors appreciate the effort of Mr. Toms Briggs in
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A.O. Majolagbe et al / Journal of Environment (2013), Vol. 02, Issue 01, pp. 19-24
the identification of plants used in this study.
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ISSN 2049-8373
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