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Monoclonal Antibody to Progesterone Receptor
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Monoclonal Antibody to Progesterone Receptor
Catalog No : ABX-140A-0.1MG
Contents : Progesterone receptor antibody at 0.2 mg/ml in PBS
with 0.05% BSA and 0.05% sodium azide. Sodium
azide is highly toxic.
Isotype : Mouse IgG1, Kappa
Clone : PR62
Protein G Chromatography was used to purify this
Purification :
progesterone receptor antibody
Species React : Human
Predicted React : Other species not tested
Host : Mouse
Application
IHC (p): use antibody at 1:500
Storage
Store antibody at 4°C, stable for 2 years. For long-term
storage, aliquot and store at -20°C.
Background
The progesterone receptor (also known as NR3C3) is a member of the steroid or nuclear hormone receptor superfamily family of proteins.
Family members are involved in diverse physiological functions including embryonic development, cell differentiation, and homeostasis. The
receptors function by binding to DNA in the nucleus and regulating the transcription of target genes. The progesterone receptor is activated
by its ligand progesterone, which is a steroid hormone involved in the menstrual cycle, pregnancy and embryogenesis. Progesterone
receptor expression status is commonly used as a marker for classifying breast cancers and for predicting prognosis and response to
endocrine therapy. That is, antibody positive versus antibody negative tumors can have potential implications with respect to predicted
outcome, treatment, or disease monitoring. The antibody reacts with the progesterone receptor. Progesterone receptor is expressed as two
major isoforms, PR-A (81 kDa) and PR-B (116 kDa). These isoforms are generated from alternative transcriptional start sites within the
progesterone receptor gene. Both forms are ligand activated by progesterone although they differ in their relative ability to activate target
gene transcription. Antibody to progesterone receptor is widely used to study breast and other progesterone receptor containing tissues such
as the endometrium. Researchers are encouraged to consult the scientific literature for additional information regarding the use of antibody to
measure progesterone receptor as part of assessing breast or other tumor types.
Antigen
A progesterone receptor recombinant protein was used as the immunogen for the antibody. The specific epitope has not been mapped.
Application Notes
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.
Formalin-fixed, paraffin-embedded normal human breast (left) and invasive ductal carcinoma (right) stained with
progesterone receptor antibody (1:500), peroxidase-conjugate and DAB chromogen.
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Monoclonal Antibody to Progesterone Receptor
Reference
1. Cserni G, M Francz, E Kalman, G Kelemen, DC Komjathy, I Kovacs, J Kulka, L Sarkadi, N Udvarhelyi, L Vass, A Voros. Pathol Oncol Res 17:663-668 (2011).
2. Mote P, JF Johnston, T Manninen, P Tuohimaa, CL Clarke. J Clin Pathol 54:624-630 (2001).
3. Mylonas I, J Makovitzky, K Firese, U Jeschke. Acta Histochemica 111:349-359 (2009).
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Fax: 1-858-642-0937
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[email protected]
Research purposes only. Not for diagnostic or in vivo use.
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